Mechanism of Phage-induced Lysis in Pneumococci
... Expression of PAL activity in Dp-1 infected cultures of the autolysin-defective mutant The PAL activity was fully expressed in cwl cultures treated with 5-fluorodeoxyuridine in spite of the fact that the titre of viable progeny phage was suppressed by about 65% (Fig. 4). Addition of streptomycin to ...
... Expression of PAL activity in Dp-1 infected cultures of the autolysin-defective mutant The PAL activity was fully expressed in cwl cultures treated with 5-fluorodeoxyuridine in spite of the fact that the titre of viable progeny phage was suppressed by about 65% (Fig. 4). Addition of streptomycin to ...
ATM and - Cancer Research
... on IR-induced p53 and Rad17 phosphorylation by immunofluorescence using phospho-specific antibodies (anti-p53Ser15 and antiRad17Ser645) and observed similar levels of phosphorylated p53 and Rad17 in control and drug-treated control cells but impaired phosphorylation in AT1BR (data not shown). Taken ...
... on IR-induced p53 and Rad17 phosphorylation by immunofluorescence using phospho-specific antibodies (anti-p53Ser15 and antiRad17Ser645) and observed similar levels of phosphorylated p53 and Rad17 in control and drug-treated control cells but impaired phosphorylation in AT1BR (data not shown). Taken ...
Chapter 3 Cells and Tissues
... Every human cell has a designated function— some help maintain the cell; others regulate life processes Specialized functions of a cell depend on number and types of organelles ...
... Every human cell has a designated function— some help maintain the cell; others regulate life processes Specialized functions of a cell depend on number and types of organelles ...
Different Roles for Simple-Cell and Complex
... all model behavior by decreasing the background inputs to complex cells (we kept these identical to all cortical cells for simplicity) or by including inhibitory chemical synapses between complex cells (Tamas et al., 1997, 1998; Gibson et al., 1999; Galarreta and Hestrin, 2002) or lowering the stren ...
... all model behavior by decreasing the background inputs to complex cells (we kept these identical to all cortical cells for simplicity) or by including inhibitory chemical synapses between complex cells (Tamas et al., 1997, 1998; Gibson et al., 1999; Galarreta and Hestrin, 2002) or lowering the stren ...
Open Access - Scientific Research Publishing
... Usually, plant cells have relatively rigid cell walls that are tightly joined to the adjacent cell walls maintaining the tissue integrity. The growing plant cells have been assumed to be hyper-rectangles with a defined aspect ratio of the longest side to the shortest side. The well-described develop ...
... Usually, plant cells have relatively rigid cell walls that are tightly joined to the adjacent cell walls maintaining the tissue integrity. The growing plant cells have been assumed to be hyper-rectangles with a defined aspect ratio of the longest side to the shortest side. The well-described develop ...
Glial Cell Inhibition of Neurons by Release of ATP
... reduced the firing rate of those neurons that displayed spontaneous spike activity. The inhibition was abolished by the A1 adenosine receptor antagonist DPCPX (8-cyclopentyl-1,3-dipropylxanthine) (10 nM) and was reduced by the ecto-ATPase inhibitor ARL-67156 (6-N,N-diethyl-D-,␥-dibromomethyleneATP) ...
... reduced the firing rate of those neurons that displayed spontaneous spike activity. The inhibition was abolished by the A1 adenosine receptor antagonist DPCPX (8-cyclopentyl-1,3-dipropylxanthine) (10 nM) and was reduced by the ecto-ATPase inhibitor ARL-67156 (6-N,N-diethyl-D-,␥-dibromomethyleneATP) ...
Platelet Adhesion to Exposed Endothelial Cell Extracellular Matrixes
... morphometrically evaluated, von Willebrand factor (vWF), fibronectin (FN), and 13-hydroxy9-cis,ll-
... morphometrically evaluated, von Willebrand factor (vWF), fibronectin (FN), and 13-hydroxy9-cis,ll-
Liver cytokine production and ICAM
... bile and serum of patients with sepsis, measurement of circulating ICAM-1 facilitates the identification of those patients with the highest risk of developing liver dysfunction (43). These data thus support the hypothesis that adhesion molecules are essential for producing inflammatory liver injury. ...
... bile and serum of patients with sepsis, measurement of circulating ICAM-1 facilitates the identification of those patients with the highest risk of developing liver dysfunction (43). These data thus support the hypothesis that adhesion molecules are essential for producing inflammatory liver injury. ...
Antibodies in plantscience
... Applications of antibody/antigen detection systems in plant biochemistry/molecular ...
... Applications of antibody/antigen detection systems in plant biochemistry/molecular ...
DNA Polymerase Regulates Cisplatin
... platinated by treatment with cisplatin before transfection. By comparing luciferase expression in the uninjured 9N58 and 6I cells it is possible to assess what fraction of host cell luciferase expression is due to specifically polymerase -mediated adduct bypass as opposed to translesional synthesis ...
... platinated by treatment with cisplatin before transfection. By comparing luciferase expression in the uninjured 9N58 and 6I cells it is possible to assess what fraction of host cell luciferase expression is due to specifically polymerase -mediated adduct bypass as opposed to translesional synthesis ...
Biochemistry of bone
... maintenance of the skeleton – modeling and remodeling healing of the skeleton (soft-tissue tissue trauma, fracture, response to neoplasma, infection) ...
... maintenance of the skeleton – modeling and remodeling healing of the skeleton (soft-tissue tissue trauma, fracture, response to neoplasma, infection) ...
Ion-channel blocker sensitivity of voltage-gated
... positively charged amino acid residues, each of which is followed by two hydrophobic residues; and the segment acts as a voltage sensor. The yeast Saccharomyces cerevisiae has one orthologue, termed Cch1, of the pore-forming a1 subunit of mammalian VGCCs (Fischer et al., 1997; Paidhungat & Garrett, ...
... positively charged amino acid residues, each of which is followed by two hydrophobic residues; and the segment acts as a voltage sensor. The yeast Saccharomyces cerevisiae has one orthologue, termed Cch1, of the pore-forming a1 subunit of mammalian VGCCs (Fischer et al., 1997; Paidhungat & Garrett, ...
Induction of wound-periderm-like tissue in
... (T6), anticlinal and periclinal divisions occupied more continuous regions and, as of the tenth day (T10), the wound-periderm began to be formed (Fig. 4D). The tissue constitution of the leaf blade (Fig. 4E) is similar to that of the petiole, with phellogen (Fig. 4E, ellipse), phellem (Fig. 3B, star ...
... (T6), anticlinal and periclinal divisions occupied more continuous regions and, as of the tenth day (T10), the wound-periderm began to be formed (Fig. 4D). The tissue constitution of the leaf blade (Fig. 4E) is similar to that of the petiole, with phellogen (Fig. 4E, ellipse), phellem (Fig. 3B, star ...
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... Over the years it was reported repeatedly that also protein hormones, cytokines, and growth factors were found intracellularly, usually in the nucleus. Many of these claims were based on data obtained with immunofluorescence or cell fractionation, approaches that can easily give misleading results, ...
... Over the years it was reported repeatedly that also protein hormones, cytokines, and growth factors were found intracellularly, usually in the nucleus. Many of these claims were based on data obtained with immunofluorescence or cell fractionation, approaches that can easily give misleading results, ...
Control of pathfinding by the avian trunk neural crest
... the sclerotome has dispersed. Finally, other neural crest cells may enter the sclerotome by migrating along the ventral root motor fibres that also course through the anterior somite (see below). Some of the crest cells migrate between the neural tube and somite opposite the posterior half of a somi ...
... the sclerotome has dispersed. Finally, other neural crest cells may enter the sclerotome by migrating along the ventral root motor fibres that also course through the anterior somite (see below). Some of the crest cells migrate between the neural tube and somite opposite the posterior half of a somi ...
Examination of the role of signal transduction and oxidative stress in
... MATERIALS AND METHODS RESULTS DISCUSSION ...
... MATERIALS AND METHODS RESULTS DISCUSSION ...
A Novel Recombinant Plasma Membrane
... referred to as pmeLUC) is shown in Figure 1A. This chimeric protein, thanks to the folate receptor leader sequence, is targeted to the plasma membrane and detects ATP in the extracellular milieu close to the cell surface (Figure 1B). Immunofluorescence and FACS analysis of cells transfected with thi ...
... referred to as pmeLUC) is shown in Figure 1A. This chimeric protein, thanks to the folate receptor leader sequence, is targeted to the plasma membrane and detects ATP in the extracellular milieu close to the cell surface (Figure 1B). Immunofluorescence and FACS analysis of cells transfected with thi ...
Long-Distance Axonal Regeneration in the Transected Adult Rat
... 1991; Bovolenta et al., 1992; Giulian, 1993; Schwab et al., 1993). Although not all adult neurons exhibit the same regenerative potential, most of them regenerate their lesioned axons if the appropriate conditions are provided. Injured axons are able to grow for long distances through pieces of peri ...
... 1991; Bovolenta et al., 1992; Giulian, 1993; Schwab et al., 1993). Although not all adult neurons exhibit the same regenerative potential, most of them regenerate their lesioned axons if the appropriate conditions are provided. Injured axons are able to grow for long distances through pieces of peri ...
Final published version - Discovery
... scale and configuration of cell populations required for tissue selforganisation and generation (Baillie-Johnson et al., 2014; van den Brink et al., 2014). The use of NMps derived from human pluripotent cells in this context might also advance tissue engineering for therapeutic purposes. For example ...
... scale and configuration of cell populations required for tissue selforganisation and generation (Baillie-Johnson et al., 2014; van den Brink et al., 2014). The use of NMps derived from human pluripotent cells in this context might also advance tissue engineering for therapeutic purposes. For example ...
Host Immune Modulation Mycobacterial Capsular Polysaccharides
... escapes its containment and causes active disease (2, 3). The interaction between M. tuberculosis and the host immune system is very complex (4, 5). An important question is how the bacillus survives its hostile environment, that is, the intracellular compartments of the macrophage, and thereby is a ...
... escapes its containment and causes active disease (2, 3). The interaction between M. tuberculosis and the host immune system is very complex (4, 5). An important question is how the bacillus survives its hostile environment, that is, the intracellular compartments of the macrophage, and thereby is a ...
analysis of the significance of a periodic, cell size
... The 3 diploid strains of different mean sizes all double their rate of poIy(A)+mRNA synthesis at points in their cell cycles when they have very similar protein contents per cell (Fraser & Nurse, 19786). Similarly, the 2 haploid strains have similar protein contents per cell when they double their r ...
... The 3 diploid strains of different mean sizes all double their rate of poIy(A)+mRNA synthesis at points in their cell cycles when they have very similar protein contents per cell (Fraser & Nurse, 19786). Similarly, the 2 haploid strains have similar protein contents per cell when they double their r ...
YSK1 is activated by the Golgi matrix protein GM130 and plays a
... Phosphorylation at the equivalent position of the T-loop in many other kinases is an important determinant for their activation (Russo et al., 1996). Mutation of the T-loop threonine to alanine to give YSK1T174A resulted in a form of the kinase that showed no activity toward MBP in the presence or a ...
... Phosphorylation at the equivalent position of the T-loop in many other kinases is an important determinant for their activation (Russo et al., 1996). Mutation of the T-loop threonine to alanine to give YSK1T174A resulted in a form of the kinase that showed no activity toward MBP in the presence or a ...
Individual-based modelling of growth and migration of Salmonella
... remains unclear. It was generally believed that the majority of vertical contaminations occurred in the albumen (Humphrey et al., 1991), but recently there has been increasing evidence for contamination of the yolk (Gast et al., 2002), particularly its membrane (Gast and Holt, 2001b). Upon horizonta ...
... remains unclear. It was generally believed that the majority of vertical contaminations occurred in the albumen (Humphrey et al., 1991), but recently there has been increasing evidence for contamination of the yolk (Gast et al., 2002), particularly its membrane (Gast and Holt, 2001b). Upon horizonta ...
Japanese encephalitis virus replication is negatively regulated by
... China, and India.30 JEV is a neurotropic virus and clinical manifestations of the disease range from febrile syndromes to multifocal CNS disorders and death.30 In the present study we have explored the relevance of autophagy and the role of the crucial autophagy protein LC3 in the context of JEV rep ...
... China, and India.30 JEV is a neurotropic virus and clinical manifestations of the disease range from febrile syndromes to multifocal CNS disorders and death.30 In the present study we have explored the relevance of autophagy and the role of the crucial autophagy protein LC3 in the context of JEV rep ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.