Diazonamide A and a Synthetic Structural Analog
... equivalent amount of dimethyl sulfoxide [the drug solvent, final concentration 0.01% (v/v)] was added; after another 24 h of growth, cells were harvested by trypsinization. The cells were washed three times with cold phosphate-buffered saline and fixed in ethanol at 0°C. Subsequently the cells were ...
... equivalent amount of dimethyl sulfoxide [the drug solvent, final concentration 0.01% (v/v)] was added; after another 24 h of growth, cells were harvested by trypsinization. The cells were washed three times with cold phosphate-buffered saline and fixed in ethanol at 0°C. Subsequently the cells were ...
Modeling leaf venation patterns Anne-Gaelle Rolland
... enough information may now be available to model these patterns in a realistic way. In this context, we have been working on biologically realistic models of auxin transport and venation formation, and plan to incoporate growth in the models in order to generate venation patterns on a developing lea ...
... enough information may now be available to model these patterns in a realistic way. In this context, we have been working on biologically realistic models of auxin transport and venation formation, and plan to incoporate growth in the models in order to generate venation patterns on a developing lea ...
05 October 2000
... incompatible pollen tubes growing in vivo, we note that genotype-independent accumulation of S-RNases in pollen tubes germinated in vitro in solutions of purified SRNases has been previously reported14. However, in these studies, both pollen-tube RNA degradation14 and growth arrest15 were genotype-i ...
... incompatible pollen tubes growing in vivo, we note that genotype-independent accumulation of S-RNases in pollen tubes germinated in vitro in solutions of purified SRNases has been previously reported14. However, in these studies, both pollen-tube RNA degradation14 and growth arrest15 were genotype-i ...
Reshaping You: Options for Breast Reconstruction
... breast that has been surgically removed. This is done using an implant or tissue from another part of your body. The goal of reconstruction is to make breasts look natural and balanced when you are wearing clothing. Can I have breast reconstruction? You may be able to have breast reconstruction, if ...
... breast that has been surgically removed. This is done using an implant or tissue from another part of your body. The goal of reconstruction is to make breasts look natural and balanced when you are wearing clothing. Can I have breast reconstruction? You may be able to have breast reconstruction, if ...
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... t Number of disaggregated cells showing FITC-Con A binding pattern. NP = n o n polar, P = polar, NS = not scorable. A cell was designated as polar if FITC-Con A fluorescence was restricted to 7 5 % or less of the cell surface (see Reeve & Ziomek, 1980). Numbers in parentheses represent additional ce ...
... t Number of disaggregated cells showing FITC-Con A binding pattern. NP = n o n polar, P = polar, NS = not scorable. A cell was designated as polar if FITC-Con A fluorescence was restricted to 7 5 % or less of the cell surface (see Reeve & Ziomek, 1980). Numbers in parentheses represent additional ce ...
Cellular Biology - Circulation Research
... Thoracic aorta was placed in endothelial basal medium-2 as described,25 periaortic tissue was carefully removed, and then the aorta was cleaned and sliced into 1 mm-long rings. Rings were then embedded in liquid collagen gel in 48-well plates (BD Biosciences), incubated at 37°C for 1 hour to polymer ...
... Thoracic aorta was placed in endothelial basal medium-2 as described,25 periaortic tissue was carefully removed, and then the aorta was cleaned and sliced into 1 mm-long rings. Rings were then embedded in liquid collagen gel in 48-well plates (BD Biosciences), incubated at 37°C for 1 hour to polymer ...
Regulated Expression of Vimentin cDNA in Cells in the Presence
... into more peripheral areas of the cytoplasm. Similarly, A1bers and Fuchs (1989) transiently expressed a modified keratin cDNA in epithelial cells and reported that the modified keratin protein was detected initially in filaments at a perinuclear location and then progressively along the cytoplasmic ...
... into more peripheral areas of the cytoplasm. Similarly, A1bers and Fuchs (1989) transiently expressed a modified keratin cDNA in epithelial cells and reported that the modified keratin protein was detected initially in filaments at a perinuclear location and then progressively along the cytoplasmic ...
Innate response to human cytomegalovirus pathogenesis of atherosclerosis
... enterica-infection of MΦ it has been shown that IFN-γ promotes intracellular killing of the bacteria and that response is defective in MΦ from patients with IFN-γ receptor deficiency [23, 24]. Coxiella burnetii is an intracellular bacterium that causes the Q fever, an infective endocarditis. Patient ...
... enterica-infection of MΦ it has been shown that IFN-γ promotes intracellular killing of the bacteria and that response is defective in MΦ from patients with IFN-γ receptor deficiency [23, 24]. Coxiella burnetii is an intracellular bacterium that causes the Q fever, an infective endocarditis. Patient ...
mast cells and basophils
... there are clear differences between mucosal and connective tissue mast cells and there are comparatively few circulating basophils. In addition, two distinct mast cell deficient strains of mouse have been identified, and have made an invaluable contribution to elucidating the key role of stem cell f ...
... there are clear differences between mucosal and connective tissue mast cells and there are comparatively few circulating basophils. In addition, two distinct mast cell deficient strains of mouse have been identified, and have made an invaluable contribution to elucidating the key role of stem cell f ...
Bone marrow contribution to skeletal muscle: A physiological
... LaBarge and Blau, 2002; Sherwood et al., 2004). However, these experiments have all used bone marrow transplantation, which is highly invasive, and associated ...
... LaBarge and Blau, 2002; Sherwood et al., 2004). However, these experiments have all used bone marrow transplantation, which is highly invasive, and associated ...
Inflamation of maxillary sinus
... Sharp and chronic odontogenic sinusitis of upperjawal sinus. Arthritis and arthrosis of tympanomaxillar joint. Sharp and chronic ...
... Sharp and chronic odontogenic sinusitis of upperjawal sinus. Arthritis and arthrosis of tympanomaxillar joint. Sharp and chronic ...
PHAGOLYSOSOME FORMATION IN NORMAL AND
... adherence to measured uptake . Monolayers were harvested by rinsing five times with warm PBS and the cell layers digested with 0.5 ml of 0.1 N NaOH solution . Assay for protein was carried out on 0.1-ml portions using the method of Lowry et al . (15) with crystalline egg white lysozyme as the standa ...
... adherence to measured uptake . Monolayers were harvested by rinsing five times with warm PBS and the cell layers digested with 0.5 ml of 0.1 N NaOH solution . Assay for protein was carried out on 0.1-ml portions using the method of Lowry et al . (15) with crystalline egg white lysozyme as the standa ...
Directional Gravity Sensing in Gravitropism
... tip without damaging root growth. Such decapped roots lose the gravitropic response, but the responsiveness is recovered following the regeneration of a new root cap (2). In addition, genetic manipulation to remove the Arabidopsis root cap—via root cap–specific expression of diphtheria toxin A, which ...
... tip without damaging root growth. Such decapped roots lose the gravitropic response, but the responsiveness is recovered following the regeneration of a new root cap (2). In addition, genetic manipulation to remove the Arabidopsis root cap—via root cap–specific expression of diphtheria toxin A, which ...
Every B3 Past Paper Question
... Most calves are produced by allowing selected animals to breed naturally. However, some farmers use the cloning method described in the stages above to produce calves from selected animals. Suggest one advantage and one disadvantage of producing calves using this cloning method. ...
... Most calves are produced by allowing selected animals to breed naturally. However, some farmers use the cloning method described in the stages above to produce calves from selected animals. Suggest one advantage and one disadvantage of producing calves using this cloning method. ...
Localization of TGN38 to the trans-Golgi Network
... HE Golgi complex plays a central role in the intracellular transport, processing and sorting of proteins in the secretory pathway. Morphological and functional studies have established that the Golgi complex of higher eukaryotes is organized into at least three contiguous but distinct regions: the c ...
... HE Golgi complex plays a central role in the intracellular transport, processing and sorting of proteins in the secretory pathway. Morphological and functional studies have established that the Golgi complex of higher eukaryotes is organized into at least three contiguous but distinct regions: the c ...
Preface - Clinical Virology Network
... mesenchymal stem cells, cytotoxic T-lymphocytes, T-regulatory cells, tumour derived cells. **Although islet cells are processed (isolated and purified) they are subject to time constraints akin to organs ***Limbal stem cells, keratinocytes and embryonic stem cells are usually cultured and expanded i ...
... mesenchymal stem cells, cytotoxic T-lymphocytes, T-regulatory cells, tumour derived cells. **Although islet cells are processed (isolated and purified) they are subject to time constraints akin to organs ***Limbal stem cells, keratinocytes and embryonic stem cells are usually cultured and expanded i ...
Alterations in Physical Integrity
... Wound edges do not approximate. Wound is left open until it becomes filled by scar tissue. Chance of infection is greater.Inflammatory phase is often chronic Wound filled with granulation tissue (a form of connective tissue that has a more abundant blood supply than collagen. Scarring is greater. ...
... Wound edges do not approximate. Wound is left open until it becomes filled by scar tissue. Chance of infection is greater.Inflammatory phase is often chronic Wound filled with granulation tissue (a form of connective tissue that has a more abundant blood supply than collagen. Scarring is greater. ...
GSK-3 Promotes Cell Survival, Growth, and PAX3 Levels in Human
... and parallels essential functions found in developing melanocytes (13–16). Like GSK-3, the transcription factor PAX3 is involved in both melanocyte development and melanoma. PAX3 is expressed in melanocyte precursors and drives lineage specificity by regulating the expression of genes critical for me ...
... and parallels essential functions found in developing melanocytes (13–16). Like GSK-3, the transcription factor PAX3 is involved in both melanocyte development and melanoma. PAX3 is expressed in melanocyte precursors and drives lineage specificity by regulating the expression of genes critical for me ...
Virus-induced hepatocellular carcinomas cause antigen
... because of their high immunogenicity (1, 2). It remains unclear why surveillance occasionally fails, e.g., against hepatitis B virus–associated (HBV-associated) or hepatitis C virus–associated (HCV-associated) hepatocellular carcinoma (HCC). Possible reasons could be an initial failure to induce eff ...
... because of their high immunogenicity (1, 2). It remains unclear why surveillance occasionally fails, e.g., against hepatitis B virus–associated (HBV-associated) or hepatitis C virus–associated (HCV-associated) hepatocellular carcinoma (HCC). Possible reasons could be an initial failure to induce eff ...
the morphogenetic function of a vestigial organ in the chick
... fig. 6. It thus becomes clear that in the Anura also the pronephric duct plays a large part in the initial induction of mesonephros development. The morphogenetic function of the chick pronephric duct is therefore one of some phylogenetic antiquity. At the same time, both Anura' and birds show some ...
... fig. 6. It thus becomes clear that in the Anura also the pronephric duct plays a large part in the initial induction of mesonephros development. The morphogenetic function of the chick pronephric duct is therefore one of some phylogenetic antiquity. At the same time, both Anura' and birds show some ...
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... initial site of entry, replicate initially in mucosal epithelial cells of the upper respiratory tract, and cause respiratory disease. Only EHV-1, however, can efficiently infect the cells of lymphoid tissues, peripheral blood mononuclear cells (PBMC), and endothelial cells (EC) of blood vessels in t ...
... initial site of entry, replicate initially in mucosal epithelial cells of the upper respiratory tract, and cause respiratory disease. Only EHV-1, however, can efficiently infect the cells of lymphoid tissues, peripheral blood mononuclear cells (PBMC), and endothelial cells (EC) of blood vessels in t ...
Arachidonic acid inhibits the store-operated Ca2+ current in rat liver
... the open channel directly or modulates the mechanism by which I SOC is slowly inactivated [31]. Addition of 10 µM AA to the internal solution containing 20 µM Ins(1,4,5)P3 had no effect on the development or amplitude of I SOC (Figure 2C). Under conditions used in the experiments with H4IIE cells de ...
... the open channel directly or modulates the mechanism by which I SOC is slowly inactivated [31]. Addition of 10 µM AA to the internal solution containing 20 µM Ins(1,4,5)P3 had no effect on the development or amplitude of I SOC (Figure 2C). Under conditions used in the experiments with H4IIE cells de ...
Mechanism of Phage-induced Lysis in Pneumococci
... Expression of PAL activity in Dp-1 infected cultures of the autolysin-defective mutant The PAL activity was fully expressed in cwl cultures treated with 5-fluorodeoxyuridine in spite of the fact that the titre of viable progeny phage was suppressed by about 65% (Fig. 4). Addition of streptomycin to ...
... Expression of PAL activity in Dp-1 infected cultures of the autolysin-defective mutant The PAL activity was fully expressed in cwl cultures treated with 5-fluorodeoxyuridine in spite of the fact that the titre of viable progeny phage was suppressed by about 65% (Fig. 4). Addition of streptomycin to ...
Macromolecular biophysics of the plant cell wall: Concepts and
... their native state, without prior fractionation or solubilisation. In addition, there are ways in which two of these techniques, vibrational and NMR spectroscopy, can be used to probe internal stress-strain relationships more or less directly at the molecular level. In principle, this approach, unli ...
... their native state, without prior fractionation or solubilisation. In addition, there are ways in which two of these techniques, vibrational and NMR spectroscopy, can be used to probe internal stress-strain relationships more or less directly at the molecular level. In principle, this approach, unli ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.