Viability and Cytotoxicity Assay Kits for Diverse Cell Types
... LIVE/DEAD Fixable Dead Cell Stain Kits The LIVE/DEAD Fixable Dead Cell Stain Kits (three of which were formerly named LIVE/DEAD Reduced Biohazard Cell Viability Kits #2, #3 and #4) (L23101, L23102, L23105, L34955) employ an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow ...
... LIVE/DEAD Fixable Dead Cell Stain Kits The LIVE/DEAD Fixable Dead Cell Stain Kits (three of which were formerly named LIVE/DEAD Reduced Biohazard Cell Viability Kits #2, #3 and #4) (L23101, L23102, L23105, L34955) employ an amine-reactive fluorescent dye to evaluate mammalian cell viability by flow ...
Ionic and Osmotic Effects of NaCl-Induced
... High-salt stress is a major environmental factor that limits plant growth and productivity (Boyer, 1982). The detrimental effects of high concentrations of salt on plants can be observed at the whole-plant level as the death of plants and/or decreases in productivity. Reductions in plant growth due ...
... High-salt stress is a major environmental factor that limits plant growth and productivity (Boyer, 1982). The detrimental effects of high concentrations of salt on plants can be observed at the whole-plant level as the death of plants and/or decreases in productivity. Reductions in plant growth due ...
Cell wall synthesis is necessary for membrane dynamics during
... peptide capable of polymerizing could serve a similar role. Bulge formation in these mutants therefore offers a useful system to examine candidate force-generating mechanisms responsible for membrane movements in bacteria. Most bacteria contain peptidoglycan, a rigid polymer built from disaccharide ...
... peptide capable of polymerizing could serve a similar role. Bulge formation in these mutants therefore offers a useful system to examine candidate force-generating mechanisms responsible for membrane movements in bacteria. Most bacteria contain peptidoglycan, a rigid polymer built from disaccharide ...
Production of Antimicrobial Metabolites by Bacillus subtilis
... Production of Antimicrobial Metabolites by Bacillus subtilis Immobilized in Polyacrylamide Gel M. Awais*, A. Pervez, Asim Yaqub and M.M. Shah Life Sciences Services (MA, AP, AY), and Biotechnology Programme (MMS), Department of Environmental Sciences, COMSATS Institute of Information Technology, Abb ...
... Production of Antimicrobial Metabolites by Bacillus subtilis Immobilized in Polyacrylamide Gel M. Awais*, A. Pervez, Asim Yaqub and M.M. Shah Life Sciences Services (MA, AP, AY), and Biotechnology Programme (MMS), Department of Environmental Sciences, COMSATS Institute of Information Technology, Abb ...
Targeting Membrane-Bound Viral RNA Synthesis Reveals
... activity after virus entry during an early step of the viral life cycle. Specifically, the formation of double membrane vesicles (DMVs), a hallmark of coronavirus replication, was greatly impaired upon K22 treatment accompanied by near-complete inhibition of viral RNA synthesis. K22-resistant viruse ...
... activity after virus entry during an early step of the viral life cycle. Specifically, the formation of double membrane vesicles (DMVs), a hallmark of coronavirus replication, was greatly impaired upon K22 treatment accompanied by near-complete inhibition of viral RNA synthesis. K22-resistant viruse ...
Function Antigens but with Altered Cytokine Secretion Cells To
... MHC II molecules in endosomal compartments of immature DCs. Immature DCs also express receptors for pathogen-associated compounds (e.g., TLR ligands). Encounter of TLR ligands activates DCs and triggers a maturation program characterized by: 1) transient upregulation of macrocytosis/phagocytosis fol ...
... MHC II molecules in endosomal compartments of immature DCs. Immature DCs also express receptors for pathogen-associated compounds (e.g., TLR ligands). Encounter of TLR ligands activates DCs and triggers a maturation program characterized by: 1) transient upregulation of macrocytosis/phagocytosis fol ...
Head and Neck Procedure Guide
... these deeper tissues. As a consequence of the attachment of four to six stays to deeper fascial planes and muscle, the surgeon creates a wide, yet shallow, surgical field. Deeper tissues are elevated into the surgical field. In placing the stays deeper in the neck, the retraction provided not only e ...
... these deeper tissues. As a consequence of the attachment of four to six stays to deeper fascial planes and muscle, the surgeon creates a wide, yet shallow, surgical field. Deeper tissues are elevated into the surgical field. In placing the stays deeper in the neck, the retraction provided not only e ...
Enzymes for Cell Dissociation and Lysis - Sigma
... have been shown to be less effective at digesting various types of collagen or mammalian tissue when compared to a mixture of both forms of the enzyme. Obviously the combination of true collagenase and the different native proteases, clostripain and aminopeptidases that have evolved in nature assist ...
... have been shown to be less effective at digesting various types of collagen or mammalian tissue when compared to a mixture of both forms of the enzyme. Obviously the combination of true collagenase and the different native proteases, clostripain and aminopeptidases that have evolved in nature assist ...
A Scientific, Policy and Economic Analysis
... they are capable of developing into virtually any tissue type. In the human, the blastocyst stage lasts for five or six days before there is implantation in the uterine wall, and for another few days thereafter. If the blastocyst does not implant in the uterine wall, it does not undergo further de ...
... they are capable of developing into virtually any tissue type. In the human, the blastocyst stage lasts for five or six days before there is implantation in the uterine wall, and for another few days thereafter. If the blastocyst does not implant in the uterine wall, it does not undergo further de ...
12Macromolecular
... early-headfold embryo, amnion, and chorion surrounded by the VYS. Two conceptuses were placed in a 30-ml serum bottle containing 4 ml medium, incubated for 2 hr, then treated with TPA or DMSO, and cultured up to 28 additional hr. The culture medium consisted of 80% rat serum, which was immediately c ...
... early-headfold embryo, amnion, and chorion surrounded by the VYS. Two conceptuses were placed in a 30-ml serum bottle containing 4 ml medium, incubated for 2 hr, then treated with TPA or DMSO, and cultured up to 28 additional hr. The culture medium consisted of 80% rat serum, which was immediately c ...
Apoptotic cell clearance: basic biology and therapeutic potential
... by fibroblasts in the absence of macrophages2. In fact, the formation of plasma membrane blebs (a common morphological feature of apoptosis) is required for the generation of smaller apoptotic cell fragments (known as apoptotic bodies). In multiple cell types, activation of RHO-associated protein ki ...
... by fibroblasts in the absence of macrophages2. In fact, the formation of plasma membrane blebs (a common morphological feature of apoptosis) is required for the generation of smaller apoptotic cell fragments (known as apoptotic bodies). In multiple cell types, activation of RHO-associated protein ki ...
The Putative Arabidopsis Arp2/3 Complex
... The evolutionarily conserved Arp2/3 complex has been shown to activate actin nucleation and branching in several eukaryotes, but its biological functions are not well understood in multicellular organisms. The model plant Arabidopsis provides many advantages for genetic dissection of the function of ...
... The evolutionarily conserved Arp2/3 complex has been shown to activate actin nucleation and branching in several eukaryotes, but its biological functions are not well understood in multicellular organisms. The model plant Arabidopsis provides many advantages for genetic dissection of the function of ...
Decontamination of the BD FACSAria System
... toxic effects on cell metabolism or responses. A cell sorter is often used by several operators (eg, in a flow core lab) for the analysis and sorting of a variety of samples including, but not limited to, humanor animal-derived blood products and cultured cells. These samples might be contaminated w ...
... toxic effects on cell metabolism or responses. A cell sorter is often used by several operators (eg, in a flow core lab) for the analysis and sorting of a variety of samples including, but not limited to, humanor animal-derived blood products and cultured cells. These samples might be contaminated w ...
Vesicular stomatitis virus infects resident cells of the central nervous
... 24 h p.i. microglia produced significant levels of both IL-6 and TNFα, cytokines known to be produced at high levels during viral encephalitis (Nuovo et al., 2005; Rempel et al., 2005), Maximal cytokine responses in microglia were observed at viral MOIBHK of 0.1 and 1 at 12 and 24 h p.i., respectivel ...
... 24 h p.i. microglia produced significant levels of both IL-6 and TNFα, cytokines known to be produced at high levels during viral encephalitis (Nuovo et al., 2005; Rempel et al., 2005), Maximal cytokine responses in microglia were observed at viral MOIBHK of 0.1 and 1 at 12 and 24 h p.i., respectivel ...
Identification of Gating Modes in Single Native Na Channels From
... Capacitive transients were compensated for carefully via compensation circuits containing two exponentials. Leak and residual capacitive currents were removed by subtracting averaged blank traces, which were formed exclusively from traces in the neighborhood of the actual sweep. None of the patches ...
... Capacitive transients were compensated for carefully via compensation circuits containing two exponentials. Leak and residual capacitive currents were removed by subtracting averaged blank traces, which were formed exclusively from traces in the neighborhood of the actual sweep. None of the patches ...
K-ras modulates the cell cycle via both positive and negative
... S phase of the cell cycle (Adams and Kaelin, 1995; DeGregori et al., 1995). Cyclin A and the B-type cyclins (cyclins B1 and B2), in complexes with CDK2 and CDC2, respectively, are essential for S-phase progression and for G2/M phase transition (reviewed in Pines and Hunter, 1992). The mammalian cell ...
... S phase of the cell cycle (Adams and Kaelin, 1995; DeGregori et al., 1995). Cyclin A and the B-type cyclins (cyclins B1 and B2), in complexes with CDK2 and CDC2, respectively, are essential for S-phase progression and for G2/M phase transition (reviewed in Pines and Hunter, 1992). The mammalian cell ...
Exine dehiscing induces rape microspore polarity
... broken but still partially covered the microspore. Such EDMs could develop into embryos with well-developed suspensors (Tian and Sun, 2003; Tang et al., 2006). Further studies indicated that the first division of the EDMs, unlike that in intact microspores, could be asymmetric. It was also reported ...
... broken but still partially covered the microspore. Such EDMs could develop into embryos with well-developed suspensors (Tian and Sun, 2003; Tang et al., 2006). Further studies indicated that the first division of the EDMs, unlike that in intact microspores, could be asymmetric. It was also reported ...
PDF - The Journal of Immunology
... the Cre-Lox system (16) and studied their response to the inflammatory assaults caused by LPS-induced endotoxemia. The rationale for using the LPS-induced endotoxemia model is based on the fact that LPS stimulates macrophages to produce numerous proinflammatory cytokines and, most importantly, it is ...
... the Cre-Lox system (16) and studied their response to the inflammatory assaults caused by LPS-induced endotoxemia. The rationale for using the LPS-induced endotoxemia model is based on the fact that LPS stimulates macrophages to produce numerous proinflammatory cytokines and, most importantly, it is ...
Inflammation Protein L13a in Macrophages Resolves An
... the Cre-Lox system (16) and studied their response to the inflammatory assaults caused by LPS-induced endotoxemia. The rationale for using the LPS-induced endotoxemia model is based on the fact that LPS stimulates macrophages to produce numerous proinflammatory cytokines and, most importantly, it is ...
... the Cre-Lox system (16) and studied their response to the inflammatory assaults caused by LPS-induced endotoxemia. The rationale for using the LPS-induced endotoxemia model is based on the fact that LPS stimulates macrophages to produce numerous proinflammatory cytokines and, most importantly, it is ...
Basic Fibroblast Growth Factor in Atria and Ventricles of the
... increased two- to fourfold in the presence of extracts compared with untreated controls. The apparent concentration of mitogenic activity is higher in the atrial extracts (compared with ventricular ones) in all species tested (chicken, rat, bovine, and sheep). Furthermore, extracts from chicken hear ...
... increased two- to fourfold in the presence of extracts compared with untreated controls. The apparent concentration of mitogenic activity is higher in the atrial extracts (compared with ventricular ones) in all species tested (chicken, rat, bovine, and sheep). Furthermore, extracts from chicken hear ...
The control of sexual identity in the Drosophila
... when XY germ cells are present in females (as discussed above), indicating that Sxl mutant XX germ cells are masculinized. Indeed, male-specific gene expression is observed in XX germ cells that lack Sxl (Staab et al., 1996; Wei et al., 1994), and Sxl functions to promote female germ cell developmen ...
... when XY germ cells are present in females (as discussed above), indicating that Sxl mutant XX germ cells are masculinized. Indeed, male-specific gene expression is observed in XX germ cells that lack Sxl (Staab et al., 1996; Wei et al., 1994), and Sxl functions to promote female germ cell developmen ...
Movement and Remodeling of the Endoplasmic
... latter, the formal proof for the mode of action of plant myosin tail domains remains elusive (Sparkes et al., 2008; Avisar et al., 2009). This fusion, along with other class XI tail domain fusions, does not collocate to these organelles, is present in motile puncta, and is diffuse throughout the cyt ...
... latter, the formal proof for the mode of action of plant myosin tail domains remains elusive (Sparkes et al., 2008; Avisar et al., 2009). This fusion, along with other class XI tail domain fusions, does not collocate to these organelles, is present in motile puncta, and is diffuse throughout the cyt ...
Mechanics and Modeling of Plant Cell Growth
... reversible (elastic) deformation. The relationship between irreversible and elastic cell wall deformation is explicit in the augmented growth equations (equations 1 to 4) and the interplay can be recovered from their solutions for different cases, e.g. pressure relaxations [11,16] and step changes i ...
... reversible (elastic) deformation. The relationship between irreversible and elastic cell wall deformation is explicit in the augmented growth equations (equations 1 to 4) and the interplay can be recovered from their solutions for different cases, e.g. pressure relaxations [11,16] and step changes i ...
Inflammation Protein L13a in Macrophages Resolves An
... the Cre-Lox system (16) and studied their response to the inflammatory assaults caused by LPS-induced endotoxemia. The rationale for using the LPS-induced endotoxemia model is based on the fact that LPS stimulates macrophages to produce numerous proinflammatory cytokines and, most importantly, it is ...
... the Cre-Lox system (16) and studied their response to the inflammatory assaults caused by LPS-induced endotoxemia. The rationale for using the LPS-induced endotoxemia model is based on the fact that LPS stimulates macrophages to produce numerous proinflammatory cytokines and, most importantly, it is ...
PDF
... Therefore, Emc functions to sequester bHLH proteins away from downstream target genes. Mutations within emc have documented growth defects in both the developing wing and eye (García-Alonso and García-Bellido, 1988; de Celis et al., 1995; Baonza and GarcíaBellido, 1999; Baonza et al., 2000; Bhattach ...
... Therefore, Emc functions to sequester bHLH proteins away from downstream target genes. Mutations within emc have documented growth defects in both the developing wing and eye (García-Alonso and García-Bellido, 1988; de Celis et al., 1995; Baonza and GarcíaBellido, 1999; Baonza et al., 2000; Bhattach ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.