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pdf, 1.5 MB - The Nebenführ Lab
pdf, 1.5 MB - The Nebenführ Lab

... the targeting domain of mammalian sialyltransferase (Boevink et al., 1998). Golgi stack movement may also provide the mechanism responsible for their dispersed distribution throughout the cytoplasm. At the same time, this movement imposes additional constraints on the possible mechanisms for transpo ...
Actin-dependent vacuolar occupancy of the cell determines auxin
Actin-dependent vacuolar occupancy of the cell determines auxin

... seemingly leads to the formation of multiple small luminal vacuoles (15), and several studies previously have addressed the mechanisms of vacuolar fragmentation (16, 18, 23–25). Accordingly, actin and its motor protein myosin could contribute the force required for auxin-dependent vacuolar fission a ...
The influence of chronic stress on T cell immunity
The influence of chronic stress on T cell immunity

... system (CNS) through a complex, interacting network of nerves, hormones, and neuropeptides. This interaction is thought to serve as a mechanism to fine-tune many immune responses, for instance to prevent the body from harmful excessive immune responses. CNSimmune interactions also play an important ...
Non-Targeted and Targeted Protein Movement
Non-Targeted and Targeted Protein Movement

... the first true leaves on the plant, older than those scored as region B. In addition, the distance traveled (no. of cells away) from the transfected cell was less for 2⫻GFP. Furthermore, triple GFP, 3⫻GFP (81 kD) was unable to move through plasmodesmata, irrespective of leaf age (Fig. 3, E and F). T ...
Polar auxin transport and patterning
Polar auxin transport and patterning

... inhibition that could explain the geometrically highly ordered positioning of primordia. Recently, a series of simulations that derive shoot meristem maxima from polar auxin transport properties has been published in an attempt to explain phyllotaxis (de Reuille et al. 2006; Jonsson et al. 2006; Smi ...
Membrane Bistability in Olfactory Bulb Mitral Cells
Membrane Bistability in Olfactory Bulb Mitral Cells

... detailed study of mitral cell electrophysiology. Recent investigations using rodent brain slices have elucidated neurotransmission between the ON and mitral cells (Ennis et al., 1996; Aroniadou-Anderjaska et al., 1997; Ciombor et al., 1999) and between mitral cell lateral dendrites and granule cells ...
Targeting of Salmonella typhimurium to Vesicles Containing
Targeting of Salmonella typhimurium to Vesicles Containing

... transport newly synthesized soluble enzymes containing M6P from the TGN to late endosomes via clathrin-coated vesicles (von Figura and Hasilik, 1986; Kornfeld, 1987, 1992; Kornfeld and MeUman, 1989). Many intracellular pathogens interact with the prelysosomal and lysosomal compartments of phagocytic ...
FEMS Microbiology Ecology.
FEMS Microbiology Ecology.

... bacterium of 3-month-old ‘Picual’, nursery-propagated olives (Mercado-Blanco et al., 2004), as well as able to colonize and persist in roots of 18–24-month-old ‘Cornicabra’, ‘Hojiblanca’, ‘Lechı́n’, ‘Manzanilla’ and ‘Picual’ olives (E. Tejedor-González, unpublished data). In addition, it has been d ...
Mochii - Salamander Genome Project
Mochii - Salamander Genome Project

... Nerve-dependent regeneration is well documented in the limbs of both urodeles and anurans, and in the tails of urodeles (Goldfarb 1909; Dinsmore & Mescher 1998). Studies of the nerve-derived soluble factors have resulted in the identification of neurotrophic factors or growth factors. Ablation of th ...
Primary cilia of human endothelial cells disassemble under laminar
Primary cilia of human endothelial cells disassemble under laminar

... To confirm the identification of IFT-71 as an IFT protein in Chlamydomonas, we prepared a polyclonal antibody to his-tagged IFT-71 (hisIFT-71Ab) and used the antibody to analyze flagellar extracts and whole cells. The sedimentation profile of IFT-71 from a 35S-labeled flagellar extract, as revealed ...
Tracheary Element Differentiation
Tracheary Element Differentiation

... of vessel development referred to in this review. TEs are only one of several xylem cell types. They are easily identified by their characteristic patterned SCW, which is confined to the lateral sides of the cell and excluded from the ends. This SCW is essential to resist the negative pressures genera ...
459 Resources
459 Resources

... • In mitochondria, the process of cellular respiration combines food molecules with oxygen. This series of chemical reactions releases energy for the cell's activities. • The energy in light is captured and stored in food molecules during the process of photosynthesis. Plants, algae, and some bacter ...
Maternal Torso-Like Coordinates Tissue Folding During
Maternal Torso-Like Coordinates Tissue Folding During

... propose that Tsl regulates extracellular Fog activity in order to synchronise cell shape changes and coordinate ventral morphogenesis in Drosophila. Identifying the Tslmediated event that is common to both terminal patterning and morphogenesis will be valuable for our understanding of the extracellu ...
Intravenously-Delivered Mesenchymal Stem Cells: Systemic Anti
Intravenously-Delivered Mesenchymal Stem Cells: Systemic Anti

... administration of stem cells. If true, then repeated injections over time would be necessary for a sustained therapeutic effect—a situation in which the intravenous route of administration would have a huge advantage over catheter-based delivery strategies. We tested these concepts in studies on mur ...
Repetitive and Prolonged Omega-3 Fatty Acid
Repetitive and Prolonged Omega-3 Fatty Acid

... Traumatic brain injury (TBI) is one of the most disabling clinical conditions that could lead to neurocognitive disorders in survivors. Our group and others previously reported that prophylactic enrichment of dietary omega-3 polyunsaturated fatty acids (n-3 PUFAs) markedly ameliorate cognitive defic ...
machen2006
machen2006

... sputum from both adult (149) and pediatric (148) CF patients have shown many (⬎40) bacterial species that have not been previously identified in CF. Most of these bacteria were metabolically active, indicating that they could potentially play a role in pathogenesis. It therefore seems possible that ...
Plant Cell
Plant Cell

... et al., 2014). In support of this hypothesis is a recent study demonstrating that the calcium dependent protein kinase 5 (CPK5) is important for the propagation of the ROS wave in plants (Romeis and Herde, 2014). ROS generated in the apoplast by an RBOHD protein of a neighboring cell (as part of the ...
Ultrastructural immunocytochemical localization of
Ultrastructural immunocytochemical localization of

... and the subsequent wash was performed as in the PAP method. Embryos were then incubated in biotinylated antirabbit IgG (Vector Laboratories Inc.), diluted 1:5 with PBS + g.s., for 1 h. After washing with PBS + g.s. for 1 h with two changes, the specimens were immersed in ferritin-congugated avidin D ...
Protruding Vulva Mutants Identify Novel Loci and Wnt
Protruding Vulva Mutants Identify Novel Loci and Wnt

... extracellular signaling pathways are known to function during this process. To identify novel loci acting in vulval development, we carried out a genetic screen for mutants having a protruding-vulva (Pvl) mutant phenotype. Here we report the initial genetic characterization of several novel loci: ba ...
Gastrulation: Making and Shaping Germ Layers
Gastrulation: Making and Shaping Germ Layers

... their subsequent patterning and diversification of cell fates along the embryonic axes. These processes occur very early during development when most embryos consist of a relatively small number of morphologically similar cells arranged in simple structures, such as cell balls or sheets, which can be ...
Podosome-type adhesions and focal adhesions, so alike yet so
Podosome-type adhesions and focal adhesions, so alike yet so

... sites so close in their composition can be structurally and functionally so different. The underlying question is how their respective physiological or pathological roles are related to their distinct organization. ...
Extrinsic Factors Influence the Expression of Voltage
Extrinsic Factors Influence the Expression of Voltage

... coverslips coated with rat tail collagen and laminin (50 &ml; courtesy of Dr. S. Carbonetto, McGill University). The cultures were maintained for 2-4 weeks at 37°C in 95% air, 5% CO, and fed every 3-l d with the growth media described above. In some experiments, recordings were done on acutely disso ...
Tendon development and musculoskeletal assembly: emerging
Tendon development and musculoskeletal assembly: emerging

... connectivity lies, within the TPCs or within the muscles themselves? Chick-quail chimera studies suggests that, at least for cranial and limb muscles, the TPCs and tendon matrix determine the pattern of attachments (Kardon, 1998; Kieny and Chevallier, 1979; Noden, 1988). Do muscles and the myomatrix ...
300 - Association of Surgical Technologists
300 - Association of Surgical Technologists

... severance between the healthy and necrotic tis­ microorganisms that cause tissue to swell and sue, ultimately resulting in the gangrenous tissue emit a fetid smell. Wet gangrene usually develops falling off if it is not surgically removed. rapidly due to blockage of venous and/or arterial If the blo ...
HIV-1 Evolves into a Nonsyncytium-Inducing Virus upon Prolonged
HIV-1 Evolves into a Nonsyncytium-Inducing Virus upon Prolonged

... Passage of HIV-1 in cell lines will affect the biological properties of the virus. Cell culture will select certain phenotypes from the quasispecies population. For example, positive selection pressure will favor variants that are better able to bind to and enter the target cells, and negative press ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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