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... To investigate the cause of the perinatal lethality observed in the Ets1-deficient mice, we performed histological analysis on twelve E16.5 to postnatal day (P) 0 Ets1–/– animals (Fig. 1). Eleven of the twelve animals examined showed a membranous ventricular septal defect, which was likely to contri ...
... To investigate the cause of the perinatal lethality observed in the Ets1-deficient mice, we performed histological analysis on twelve E16.5 to postnatal day (P) 0 Ets1–/– animals (Fig. 1). Eleven of the twelve animals examined showed a membranous ventricular septal defect, which was likely to contri ...
Passive transfer of anti-herpes simplex virus type 2
... excised and incubated in 5 mmol/1 ethylenediaminetetraacetic acid (EDTA) for 20 min in 5% carbon dioxide at 37°C. Using forceps the epithelial sheets were lifted off the stromal-endothelial portions of the cornea and incubated with 0.1% trypsin for 5 min in 5% carbon dioxide at 37°C. The epithelial ...
... excised and incubated in 5 mmol/1 ethylenediaminetetraacetic acid (EDTA) for 20 min in 5% carbon dioxide at 37°C. Using forceps the epithelial sheets were lifted off the stromal-endothelial portions of the cornea and incubated with 0.1% trypsin for 5 min in 5% carbon dioxide at 37°C. The epithelial ...
The viability assessment of Microcystis aeruginosa cells after co-culturing
... used to assess the viability of Microcystis spp. cells after deliberate co-culturing with a predatory bacterium, Bacillus mycoides B16. Under static conditions, B. mycoides had a lytic effect on Microcystis cells that resulted in a significant (p = 0.0000) population decline of 97% in six days. In co ...
... used to assess the viability of Microcystis spp. cells after deliberate co-culturing with a predatory bacterium, Bacillus mycoides B16. Under static conditions, B. mycoides had a lytic effect on Microcystis cells that resulted in a significant (p = 0.0000) population decline of 97% in six days. In co ...
Nitric Oxide Acts as an Antioxidant and Delays Programmed Cell
... Nitric oxide (NO) is a freely diffusible, gaseous free radical and an important signaling molecule in animals. In plants, NO influences aspects of growth and development, and can affect plant responses to stress. In some cases, the effects of NO are the result of its interaction with reactive oxygen ...
... Nitric oxide (NO) is a freely diffusible, gaseous free radical and an important signaling molecule in animals. In plants, NO influences aspects of growth and development, and can affect plant responses to stress. In some cases, the effects of NO are the result of its interaction with reactive oxygen ...
Cellular source and molecular form of TNF specify
... were immunized with i.p. SRBCs and spleen sections analyzed on day 8. B-TNF KO mice display reduced GCs, lack of polarized B-cell follicles, and disruption of FDC networks. Note that some FDC- and PNA-positive cells are present in the spleens of B-TNF KO mice (shown by arrows), while no FDCs and no ...
... were immunized with i.p. SRBCs and spleen sections analyzed on day 8. B-TNF KO mice display reduced GCs, lack of polarized B-cell follicles, and disruption of FDC networks. Note that some FDC- and PNA-positive cells are present in the spleens of B-TNF KO mice (shown by arrows), while no FDCs and no ...
Photosynthesis Research
... slide surfaces have caused about 15 grana to become sufficiently detached as to form a loop extending away from the main body of the chloroplast. Six grana in the loop are very conspicuous, and two arrows point to fine threads connecting grana just at the limit of resolution of the phase microscope. T ...
... slide surfaces have caused about 15 grana to become sufficiently detached as to form a loop extending away from the main body of the chloroplast. Six grana in the loop are very conspicuous, and two arrows point to fine threads connecting grana just at the limit of resolution of the phase microscope. T ...
Comparison of the Leukocyte differentiation
... 2 channels have different patterns due to the differences in the reagents used as well as differences in the hardware and software. In particular, the WDF channel differentiates between lymphocytes and monocytes and enhances the separation capacity, thereby distinguishing it from the DIFF channel. I ...
... 2 channels have different patterns due to the differences in the reagents used as well as differences in the hardware and software. In particular, the WDF channel differentiates between lymphocytes and monocytes and enhances the separation capacity, thereby distinguishing it from the DIFF channel. I ...
Competition with pathogens in mucosal colonization
... pathogens from the vaginal mucosa. Multiple components of the bacterial cell surface seem to participate in the adherence of the strains to vaginal epithelial cells. In L. acidophilus and L. gasseri, adherence involved proteins and carbohydrate (possibly a glycoprotein), while L. jensenii adherence ...
... pathogens from the vaginal mucosa. Multiple components of the bacterial cell surface seem to participate in the adherence of the strains to vaginal epithelial cells. In L. acidophilus and L. gasseri, adherence involved proteins and carbohydrate (possibly a glycoprotein), while L. jensenii adherence ...
Planar cell polarity signalling regulates cell adhesion properties in
... progenitor cell specification followed by a morphogenetic phase that gradually integrates progenitor cells into a functional supracellular structure (Wolpert, 2002). Tight coordination of adhesion, shape changes, polarisation and epithelialisation of progenitor cells is central to this process (Brya ...
... progenitor cell specification followed by a morphogenetic phase that gradually integrates progenitor cells into a functional supracellular structure (Wolpert, 2002). Tight coordination of adhesion, shape changes, polarisation and epithelialisation of progenitor cells is central to this process (Brya ...
Chloroplasts in living cells and the string-of
... slide surfaces have caused about 15 grana to become sufficiently detached as to form a loop extending away from the main body of the chloroplast. Six grana in the loop are very conspicuous, and two arrows point to fine threads connecting grana just at the limit of resolution of the phase microscope. ...
... slide surfaces have caused about 15 grana to become sufficiently detached as to form a loop extending away from the main body of the chloroplast. Six grana in the loop are very conspicuous, and two arrows point to fine threads connecting grana just at the limit of resolution of the phase microscope. ...
Functional Utrastructure of Genlisea (Lentibulariaceae) Digestive
... in Pinguicula, Utricularia and Genlisea, revealing structural and topographical differences and similarities, can shed light on the evolution of the carnivorous syndrome in Lentibulariaceae. It is well known that chemical fixation of biological material often fails to rapidly stabilize all cell comp ...
... in Pinguicula, Utricularia and Genlisea, revealing structural and topographical differences and similarities, can shed light on the evolution of the carnivorous syndrome in Lentibulariaceae. It is well known that chemical fixation of biological material often fails to rapidly stabilize all cell comp ...
ABSTRACT SUSTAINED DELIVERY AND PHARMACODYNAMICS OF AN INTEGRIN
... therapies. Development of novel therapeutic agents provides effective treatment for ocular disorders. However, treatment of many posterior segment diseases like agerelated macular degeneration (AMD) and diabetic retinopathy (DRP) is far from satisfactory due to the limited availability of novel ther ...
... therapies. Development of novel therapeutic agents provides effective treatment for ocular disorders. However, treatment of many posterior segment diseases like agerelated macular degeneration (AMD) and diabetic retinopathy (DRP) is far from satisfactory due to the limited availability of novel ther ...
A c a d
... coronally and is capable of both necrosis and some regeneration by cementoblasts. Both the periodontal ligament and gingiva anchor fibres into the cementum. There are basically two varieties of cementum distinguished on the basis of the presence or absence of cells within it and the origin of the co ...
... coronally and is capable of both necrosis and some regeneration by cementoblasts. Both the periodontal ligament and gingiva anchor fibres into the cementum. There are basically two varieties of cementum distinguished on the basis of the presence or absence of cells within it and the origin of the co ...
Mapping the route from naive pluripotency to lineage specification
... controlled conditional genetic perturbations and real-time reporters of gene expression and signalling pathway activity. These types of studies are limited by poor accessibility of peri- and postimplantation stages in utero, sub-optimal development of early postimplantation embryos ex vivo and low c ...
... controlled conditional genetic perturbations and real-time reporters of gene expression and signalling pathway activity. These types of studies are limited by poor accessibility of peri- and postimplantation stages in utero, sub-optimal development of early postimplantation embryos ex vivo and low c ...
Subthreshold High-Frequency Electrical Field
... PPA, Colbe, Germany), and 1% penicillin–streptomycin (P-4458; Sigma-Aldrich, Steinheim, Germany). Myocytes were purified from fibroblasts by passage through a Percoll gradient (P-1644; Sigma-Aldrich). Myocytes were plated onto six-well dishes (140685; Nunc, Schwerte, Germany) at a density of 3 × 105 ...
... PPA, Colbe, Germany), and 1% penicillin–streptomycin (P-4458; Sigma-Aldrich, Steinheim, Germany). Myocytes were purified from fibroblasts by passage through a Percoll gradient (P-1644; Sigma-Aldrich). Myocytes were plated onto six-well dishes (140685; Nunc, Schwerte, Germany) at a density of 3 × 105 ...
MESOPHYLL STRUCTURE AND CHLOROPLAST DENSITY IN
... layer of palisade cells which commonly are not cylindrical but rather oval or conical. This is not the case in ginseng. Oxalis acetosella L., considered the most shade-tolerant species among the herbaceous plants, is similar to P. ginseng in number of plastids per leaf volume (Tab. 2). Another sciop ...
... layer of palisade cells which commonly are not cylindrical but rather oval or conical. This is not the case in ginseng. Oxalis acetosella L., considered the most shade-tolerant species among the herbaceous plants, is similar to P. ginseng in number of plastids per leaf volume (Tab. 2). Another sciop ...
Molecular and Cellular Mechanisms Whereby the Apical Ectodermal
... outward from the body wall, differences along each axis can be observed which allow for normal function of the vertebrate limb: the proximal bone structure is much different than the distal bone structure, the formation of tendons on the dorsal side of the autopod compared to the ventral side allow ...
... outward from the body wall, differences along each axis can be observed which allow for normal function of the vertebrate limb: the proximal bone structure is much different than the distal bone structure, the formation of tendons on the dorsal side of the autopod compared to the ventral side allow ...
Formation of Enzymes of Autotrophic Metabolism
... not allowed to drop below 10 kPa (0.1 atm) during growth on organic substrates and was kept between 2 and 8 kPa during autotrophic growth with H, and CO,. The 0, content of the exit gas of the fermenter was determined with a paramagnetic 0, analyser (Magnos 2T; Hartmann & Braun, Frankfurt, F.R.G.), ...
... not allowed to drop below 10 kPa (0.1 atm) during growth on organic substrates and was kept between 2 and 8 kPa during autotrophic growth with H, and CO,. The 0, content of the exit gas of the fermenter was determined with a paramagnetic 0, analyser (Magnos 2T; Hartmann & Braun, Frankfurt, F.R.G.), ...
The carbohydrates ofPhaeocystisand their degradation in the
... produced by colonial Phaeocystis cells. In all plants photosynthetic rates exceed metabolic demands when irradiance is optimal. The excess energy thus harvested is stored mainly as glucan. This glucan can be used as a respiratory substrate to maintain growth when irradiance is suboptimal, for exampl ...
... produced by colonial Phaeocystis cells. In all plants photosynthetic rates exceed metabolic demands when irradiance is optimal. The excess energy thus harvested is stored mainly as glucan. This glucan can be used as a respiratory substrate to maintain growth when irradiance is suboptimal, for exampl ...
Apical constriction
... constriction is also associated with the extrusion of apoptotic or delaminating cells (Toyama et al., 2008; Slattum et al., 2009; Marinari et al., 2012) and wound contraction and healing (Davidson et al., 2002; Antunes et al., 2013). Thus, apical constriction remodels epithelia in a variety of ways ...
... constriction is also associated with the extrusion of apoptotic or delaminating cells (Toyama et al., 2008; Slattum et al., 2009; Marinari et al., 2012) and wound contraction and healing (Davidson et al., 2002; Antunes et al., 2013). Thus, apical constriction remodels epithelia in a variety of ways ...
Apical constriction: themes and variations on a cellular mechanism
... constriction is also associated with the extrusion of apoptotic or delaminating cells (Toyama et al., 2008; Slattum et al., 2009; Marinari et al., 2012) and wound contraction and healing (Davidson et al., 2002; Antunes et al., 2013). Thus, apical constriction remodels epithelia in a variety of ways ...
... constriction is also associated with the extrusion of apoptotic or delaminating cells (Toyama et al., 2008; Slattum et al., 2009; Marinari et al., 2012) and wound contraction and healing (Davidson et al., 2002; Antunes et al., 2013). Thus, apical constriction remodels epithelia in a variety of ways ...
CML cells actively evade host immune surveillance
... The development of tyrosine kinase inhibitors (TKIs) to target BCR-ABL kinase has revolutionized the management of chronic phase CML with many patients now predicted to have a normal life expectancy.1, 2 Remission is maintained by continuous administration of TKI and assessed by quantification of BC ...
... The development of tyrosine kinase inhibitors (TKIs) to target BCR-ABL kinase has revolutionized the management of chronic phase CML with many patients now predicted to have a normal life expectancy.1, 2 Remission is maintained by continuous administration of TKI and assessed by quantification of BC ...
Efficient Procedure and Methods to Determine Critical
... named electro-chemotherapy [5-7] and gene electro-transfer [8,9], respectively. In the past decade, non-thermal irreversible electroporation for the ablation of solid tumors has emerged as a new medical application of electroporation technology [3, 10]. In the food and pharmaceutical application, el ...
... named electro-chemotherapy [5-7] and gene electro-transfer [8,9], respectively. In the past decade, non-thermal irreversible electroporation for the ablation of solid tumors has emerged as a new medical application of electroporation technology [3, 10]. In the food and pharmaceutical application, el ...
pdf, 1.5 MB - The Nebenführ Lab
... the targeting domain of mammalian sialyltransferase (Boevink et al., 1998). Golgi stack movement may also provide the mechanism responsible for their dispersed distribution throughout the cytoplasm. At the same time, this movement imposes additional constraints on the possible mechanisms for transpo ...
... the targeting domain of mammalian sialyltransferase (Boevink et al., 1998). Golgi stack movement may also provide the mechanism responsible for their dispersed distribution throughout the cytoplasm. At the same time, this movement imposes additional constraints on the possible mechanisms for transpo ...
The influence of chronic stress on T cell immunity
... system (CNS) through a complex, interacting network of nerves, hormones, and neuropeptides. This interaction is thought to serve as a mechanism to fine-tune many immune responses, for instance to prevent the body from harmful excessive immune responses. CNSimmune interactions also play an important ...
... system (CNS) through a complex, interacting network of nerves, hormones, and neuropeptides. This interaction is thought to serve as a mechanism to fine-tune many immune responses, for instance to prevent the body from harmful excessive immune responses. CNSimmune interactions also play an important ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.