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Journal of Phycology
Journal of Phycology

... filtration onto GF/F (Whatman, Florham Park, NJ, USA) filters, by centrifugation, or by addition of aqueous samples directly to the extraction solvents. Lipids were extracted using a modified Bligh and Dyer procedure (Bligh and Dyer 1959). Briefly, filters or pellets were immediately placed in 10:5: ...
LvNotch positions the ectoderm-endoderm boundary
LvNotch positions the ectoderm-endoderm boundary

... ectoderm (D. R. Sherwood, PhD thesis, Duke University, Durham, NC, 1997; Logan et al., 1999), suggesting a possible later function in regulating the position of the ectodermendoderm boundary. The sea urchin homolog of the Notch receptor, LvNotch, may also have a role in mediating the position of the ...
Nodal mutant eXtraembryonic ENdoderm (XEN
Nodal mutant eXtraembryonic ENdoderm (XEN

... doi: 10.1242/bio.2012038 ...
NG2 glial cells integrate synaptic input in global and dendritic
NG2 glial cells integrate synaptic input in global and dendritic

... synaptic currents originates in dendrites and its integration is difficult to study with somatic ...
Chemical Microscopy Applied to Biological Systems
Chemical Microscopy Applied to Biological Systems

... stands out the most for its definition of excitation volume, which occurs only at regions where the photon density is high; this makes the technique less susceptible to out-of-focus emission, resulting in both higher resolution and signal-to-noise ratio (5). Sánchez and Gratton used two-photon fluo ...
Light-Dependent Intracellular Positioning of Mitochondria in
Light-Dependent Intracellular Positioning of Mitochondria in

... generated from the photochemical reactions in chloroplasts (Raghavendra and Padmasree 2003, Noctor et al. 2007, Noguchi and Yoshida 2008). The well-known intimate association or co-localization of mitochondria with chloroplasts and peroxisomes may be a prerequisite for such interactions (Frederick a ...
by Bone Marrow Mesenchymal  Stem  Cell Secretions 2011
by Bone Marrow Mesenchymal Stem Cell Secretions 2011

... Doctor of Philosophy in Biomedical Engineering ...
A-new-precipitation-technique-provides-evidence-for-the
A-new-precipitation-technique-provides-evidence-for-the

... negative fixed charge of the cell wall. Cell walls provide mechanical strength to the plant, as well as functioning as a porous network involved in a diverse range of passive transport processes (gas, water, nutrient ions, assimilates). The porous network consists of intermicrofibrillar and intermic ...
1 Electrophysiology and pacemaker function of the - AJP
1 Electrophysiology and pacemaker function of the - AJP

... canine hearts the pacemaker cells are more concentrated in the proximity of the sinus node artery at the junction of the superior vena cava and the right atrium (12, 94, 99). Studies on the cytological organization of the SAN have revealed a structure composed of a dense array of connective tissue i ...
ARVO 2015 Annual Meeting Abstracts 164 Cornea endothelium
ARVO 2015 Annual Meeting Abstracts 164 Cornea endothelium

... Medical Sciences, Doshisha University, Kyotanabe, Japan. Purpose: Human corneal endothelial cells (HCECs) have poor proliferative ability under in vitro culture conditions. The tendency to enter into cell senescence or phase transition (epithelial-mesenchymal transition (EMT), cell senescence, and f ...
Localization of the Wilms` tumour protein WT1 in avian embryos
Localization of the Wilms` tumour protein WT1 in avian embryos

... were then rehydrated in TRIS-phosphate-buffered saline (TPBS) and the endogenous peroxidase activity was quenched by incubation for 30 min with 3% hydrogen peroxide in TPBS. After washing, non-specific binding sites were saturated for 30 min with 16% sheep serum, 1% bovine serum albumin and 0.5% Tri ...
A Role in Migration for the v 1 Integrin Expressed on
A Role in Migration for the v 1 Integrin Expressed on

... Figure 2. The relationship between differentiation and migration in oligodendrocyte precursor cells. Cells were resuspended at high density in agarose, as described in Materials and Methods, and then plated as small drops onto poly-DL-ornithine-coated plastic and grown in defined medium alone, witho ...
Pathogenicity and Commensalism Recognition Contribute to Fungal
Pathogenicity and Commensalism Recognition Contribute to Fungal

... and part of the normal flora (4). In addition to Candida albicans, several strains of the S. cerevisiae species have been isolated from immunocompromised individuals, such as cystic fibrosis or AIDS patients (5, 6). These findings are not in conflict with the concept that S. cerevisiae is “generally ...
Two Cortical Circuits Control Propagating Waves in Visual Cortex
Two Cortical Circuits Control Propagating Waves in Visual Cortex

... classes of neurons can be directly compared because the activities are expressed as fractions, but it should be remembered that the total numbers of neurons in each class are quite different. Thus, 70% of the subpial cell population is a smaller number (31 cells) than 30% of the pyramidal cell popul ...
An Equatorial Contractile Mechanism Drives Cell Elongation but not
An Equatorial Contractile Mechanism Drives Cell Elongation but not

... Ivonne M. Sehring., Bo Dong.¤, Elsa Denker, Punit Bhattachan, Wei Deng, Birthe T. Mathiesen, Di Jiang* Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway ...
T cell metabolism drives immunity
T cell metabolism drives immunity

... gun, pathogen-derived or inflammatory signals drive T cells out of quiescence, resulting in rapid modulation of gene expression and the acquisition of new functions.These changes range from increased production of cytokines and cytolytic molecules to the ability to undergo cell division and migratio ...
The first cell fate specification event in mouse development
The first cell fate specification event in mouse development

... Upstream  of  Cdx-­‐2   4.  Discussion   ...
Light Modulates the Biosynthesis and
Light Modulates the Biosynthesis and

... carboxysome numbers. The light dependence of carboxysome content was further substantiated by transmission electron microscopy results of wild-type Synechococcus cells (Fig. 2B; Supplemental Fig. S3). The numbers and positioning of carboxysomes in the cell were statistically analyzed based on the co ...
Cell Communication and Signaling
Cell Communication and Signaling

... yet unknown host cell proteins [13,21], as well as paxillin, an integrin-associated scaffold protein [22]. However, the functional importance of these findings for host cell entry and which integrin maybe involved in this signaling remained unclear. CadF and FlpA also seems to be involved in the act ...
Parvalbumin-Expressing Inhibitory Interneurons in Auditory Cortex
Parvalbumin-Expressing Inhibitory Interneurons in Auditory Cortex

... Introduction The responses of neurons in the auditory cortex are powerfully shaped by the relative strength and timing of excitatory and inhibitory synaptic inputs. Cortical inhibition is provided by local GABAergic interneurons, which comprise ⬃20% of the cortical population (Xu et al., 2010; but s ...
PDF
PDF

... an older environment (or vice versa) did not change cell fate acquisition or the schedule of expression of cell-specific markers (Watanabe and Raff, 1990; Belliveau and Cepko, 1999; Belliveau et al., 2000; Rapaport et al., 2001). Clonal density cell culture demonstrated that RPCs from embryonic day ...
Defining retinal progenitor cell competence in
Defining retinal progenitor cell competence in

... an older environment (or vice versa) did not change cell fate acquisition or the schedule of expression of cell-specific markers (Watanabe and Raff, 1990; Belliveau and Cepko, 1999; Belliveau et al., 2000; Rapaport et al., 2001). Clonal density cell culture demonstrated that RPCs from embryonic day ...
INTRODUCCIÓN
INTRODUCCIÓN

... Eicosanoids, including prostaglandins (PGs), leukotrienes (LTs) and lipoxins (LXs) are small fatty acid molecules derived from polyunsaturated fatty acids released from the cell membrane phospholipids, mainly araquidonic acid. Prostaglandins play a significant role in the progress of the immune resp ...
HGF and TGFβ1 differently influenced Wwox regulatory function on
HGF and TGFβ1 differently influenced Wwox regulatory function on

... from squamous cell carcinoma [13]. Although EMT seems a transient process, present at least only at the invasive front of carcinomas, while MET being the driving force for metastatic colonization [7], the molecular regulators that promote MET transition, critical for the formation of bone metastasis ...
05_Microb_biofilm_I_2014
05_Microb_biofilm_I_2014

... Microbiology lead astray – 2 The whole microbiology has been mislead by efforts to examine and investigate pure cultures of planktonically growing cells only, whereas the natural microbial growth is in the form of biofilm ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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