Protein kinase Ca activation by RET: evidence for a negative
... GPI-anchored protein named GFRa. The existence of four different GFRa proteins (GFRa1, GFRa2, GFRa3 and GFRa4) gives different ligands the specificity of binding to RET-GFRa complex. The GFRa receptors are linked to the cell membrane via glycosyl-phosphatidylinositol (GPI)-anchors. This signaling com ...
... GPI-anchored protein named GFRa. The existence of four different GFRa proteins (GFRa1, GFRa2, GFRa3 and GFRa4) gives different ligands the specificity of binding to RET-GFRa complex. The GFRa receptors are linked to the cell membrane via glycosyl-phosphatidylinositol (GPI)-anchors. This signaling com ...
Pap Smears - The Brookside Associates
... Mild dysplasia is not a permanent feature once it occurs. It can come and go, being present on a woman's cervix (and Pap smear) at one time and not another. This happens because the HPV virus that is a pre-requisite for these changes can lie dormant within the cervical skin cells. Normally held in c ...
... Mild dysplasia is not a permanent feature once it occurs. It can come and go, being present on a woman's cervix (and Pap smear) at one time and not another. This happens because the HPV virus that is a pre-requisite for these changes can lie dormant within the cervical skin cells. Normally held in c ...
ARVO 2015 Annual Meeting Abstracts 164 Cornea endothelium
... this study was to evaluate the ability of a synthetic glucorticoïd, dexamethasone (Dex), to block EndMT of cultured HCEC. Methods: HCEC (n=4 populations, passage 2) with a mixed phenotype were passaged and grown in a proliferation medium containing Dex (1 mM). The medium was changed for a maturation ...
... this study was to evaluate the ability of a synthetic glucorticoïd, dexamethasone (Dex), to block EndMT of cultured HCEC. Methods: HCEC (n=4 populations, passage 2) with a mixed phenotype were passaged and grown in a proliferation medium containing Dex (1 mM). The medium was changed for a maturation ...
KINGDOM PROTISTA
... Protozoa live just about anywhere that is moist, on both land and sea, but in this lab we will primarily be looking at protozoa that live in fresh water environments. Protozoa that live in fresh water face a problem; compared to the surrounding water the high concentration of solute in their cells c ...
... Protozoa live just about anywhere that is moist, on both land and sea, but in this lab we will primarily be looking at protozoa that live in fresh water environments. Protozoa that live in fresh water face a problem; compared to the surrounding water the high concentration of solute in their cells c ...
Identification of Primary Lysosomes in Human
... in one or two cisternae, lent to GERL (Golgi, endoplasmic some22), although some of the other ...
... in one or two cisternae, lent to GERL (Golgi, endoplasmic some22), although some of the other ...
Golgi clusters and vesicles mediate mitotic inheritance
... showed that clusters could be visualized at all stages of mitosis. At no stage were they observed to disappear. These experiments were carried out using a HeLa cell line stably transfected with GFP attached to the Golgi retention signal of N-acetylglucosaminyltransferase I (yielding NAGFP). Clusters ...
... showed that clusters could be visualized at all stages of mitosis. At no stage were they observed to disappear. These experiments were carried out using a HeLa cell line stably transfected with GFP attached to the Golgi retention signal of N-acetylglucosaminyltransferase I (yielding NAGFP). Clusters ...
Introduction to Microbiology
... Production of alcohol, making of cheese and yoghurt, and retting of flax are some of the processes mankind has harnessed since prehistoric times. Micro-organisms are also responsible for breaking down organic matter in nature. Antony van Leeuwenhoelk (1632 - 1723 ) first discovered the microbial wor ...
... Production of alcohol, making of cheese and yoghurt, and retting of flax are some of the processes mankind has harnessed since prehistoric times. Micro-organisms are also responsible for breaking down organic matter in nature. Antony van Leeuwenhoelk (1632 - 1723 ) first discovered the microbial wor ...
SYBR Green Cells-to-CT™ Control Kit
... fast cycling conditions, respectively) with user-supplied primer sets targeting genes of interest. The SYBR Green Cells-to-CT Control Kit is designed for use with the Power SYBR Green and Fast SYBR Green Cells-to-CT Kits. It includes Xeno RNA Control, a synthetic RNA transcript with a unique sequenc ...
... fast cycling conditions, respectively) with user-supplied primer sets targeting genes of interest. The SYBR Green Cells-to-CT Control Kit is designed for use with the Power SYBR Green and Fast SYBR Green Cells-to-CT Kits. It includes Xeno RNA Control, a synthetic RNA transcript with a unique sequenc ...
Comprehensive Translational Assessment of Human Induced
... blocks L-type calcium current, ranolazine blocks late sodium current) (Johannesen et al. 2014). This has been the driving factor for the development of a new Comprehensive in vitro Proarrhythmia Assay (CiPA) (Colatsky et al. 2016; Fermini et al. 2016; Sager et al. 2014). CiPA calls for a three-prong ...
... blocks L-type calcium current, ranolazine blocks late sodium current) (Johannesen et al. 2014). This has been the driving factor for the development of a new Comprehensive in vitro Proarrhythmia Assay (CiPA) (Colatsky et al. 2016; Fermini et al. 2016; Sager et al. 2014). CiPA calls for a three-prong ...
role of potassium in human and animal nutrition 2
... Ability to loose an extra electron and acquire an over all +VE charge than to gain one and acquire a negative charge. ...
... Ability to loose an extra electron and acquire an over all +VE charge than to gain one and acquire a negative charge. ...
Rapid Structural Changes and Acidification of Guard Cell Vacuoles
... We hypothesized that PtdIns(3,5)P2 plays an important role in the regulation of vacuolar acidification in guard cells, since this lipid has been reported to be essential for the acidification of yeast vacuoles (Gary et al., 1998). To test this idea, we examined the effect of a PI3P5K inhibitor (PIKfyv ...
... We hypothesized that PtdIns(3,5)P2 plays an important role in the regulation of vacuolar acidification in guard cells, since this lipid has been reported to be essential for the acidification of yeast vacuoles (Gary et al., 1998). To test this idea, we examined the effect of a PI3P5K inhibitor (PIKfyv ...
The Type II Arabidopsis Formin14 Interacts with Microtubules and
... cage around the spindle, and very few filaments remained in the spindle (Figure 3A). In addition, the microfilament band in control cells was thinner than the microtubule band in phragmoplasts (Figure 3B). Interestingly, in cells overexpressing AFH14-GFP, microfilaments codistributed with AFH14-GFP ...
... cage around the spindle, and very few filaments remained in the spindle (Figure 3A). In addition, the microfilament band in control cells was thinner than the microtubule band in phragmoplasts (Figure 3B). Interestingly, in cells overexpressing AFH14-GFP, microfilaments codistributed with AFH14-GFP ...
Anti-cancer properties of low-molecular
... Anti-cancer properties of tinzaparin and other LMWH A series of coordinated steps are pivotal for cancer development and metastasis, including proliferation of cancer cells, invasion into surrounding tissue, avoiding attack from the immune system, angiogenesis, and metastatic spread (34). The publis ...
... Anti-cancer properties of tinzaparin and other LMWH A series of coordinated steps are pivotal for cancer development and metastasis, including proliferation of cancer cells, invasion into surrounding tissue, avoiding attack from the immune system, angiogenesis, and metastatic spread (34). The publis ...
Liu_umd_0117E_10287 - University of Maryland Libraries
... His true passion for science has impressed me. What he has done sets up the great example leading me through my future endeavors. Also, I would like to thank the members of my dissertation committee: Kenneth Frauwirth, Wenxia Song, David Mosser, and Sibal Samal. I appreciate all of your suggestions, ...
... His true passion for science has impressed me. What he has done sets up the great example leading me through my future endeavors. Also, I would like to thank the members of my dissertation committee: Kenneth Frauwirth, Wenxia Song, David Mosser, and Sibal Samal. I appreciate all of your suggestions, ...
Glucose-Regulated Anaplerosis and Cataplerosis in
... 0.07% bovine serum albumin (BSA) (Fraction V, fatty acid free from Sigma), and subsequently preincubated in the same KRBH medium for a period of 30 min at 37°C. Cells were then incubated with various concentrations of glucose and test substances. Some experiments were carried out in the presence of ...
... 0.07% bovine serum albumin (BSA) (Fraction V, fatty acid free from Sigma), and subsequently preincubated in the same KRBH medium for a period of 30 min at 37°C. Cells were then incubated with various concentrations of glucose and test substances. Some experiments were carried out in the presence of ...
Functional Role of the Fast Transient Outward K Current IA in
... monitored continuously during all recordings. Only cells in which these parameters remained constant were analyzed further. Peak outward K ⫹ currents in individual cells were defined as the maximum value of the outward K ⫹ currents evoked during (300 ms or 6 s) depolarizing voltage steps. Plateau (s ...
... monitored continuously during all recordings. Only cells in which these parameters remained constant were analyzed further. Peak outward K ⫹ currents in individual cells were defined as the maximum value of the outward K ⫹ currents evoked during (300 ms or 6 s) depolarizing voltage steps. Plateau (s ...
BUFADIENOLIDES AND THEIR MEDICINAL UTILITY: A REVIEW Review Article
... hypertension, syndromes in which they are considered capable of causing a vascular leak, interfering with cellular proliferation, and inhibiting cellular maturation [54]. The cytotoxic evaluation showed that all natural bufadienolides and their derivatives exhibited moderate to strong activity again ...
... hypertension, syndromes in which they are considered capable of causing a vascular leak, interfering with cellular proliferation, and inhibiting cellular maturation [54]. The cytotoxic evaluation showed that all natural bufadienolides and their derivatives exhibited moderate to strong activity again ...
A Scientific, Policy and Economic Analysis
... research use and none being useful for clinical application because they have come into contact with mouse feeder cells. Experts predict that, at most, only four or five additional eligible lines will become available in the future. In order to develop this field of research, it is necessary to enab ...
... research use and none being useful for clinical application because they have come into contact with mouse feeder cells. Experts predict that, at most, only four or five additional eligible lines will become available in the future. In order to develop this field of research, it is necessary to enab ...
Signaling networks that regulate muscle development: Lessons from
... Fig. 1. Schematic illustrations of zebrafish muscle development. (a) Slow and fast muscle precursors occupy a distinct region in the marginal zone at the gastrula stage. Magenta dots indicate the locations of slow muscle or muscle pioneer cell precursors. Green dots indicate the locations of fast mu ...
... Fig. 1. Schematic illustrations of zebrafish muscle development. (a) Slow and fast muscle precursors occupy a distinct region in the marginal zone at the gastrula stage. Magenta dots indicate the locations of slow muscle or muscle pioneer cell precursors. Green dots indicate the locations of fast mu ...
Requirement for ß-Catenin in Anterior
... The anterior-posterior axis of the mouse embryo becomes explicit morphologically at embryonic day (E)1 6.5, when the first mesoderm forms in the primitive streak region at the posterior side of the embryo. However, recent experiments show that anterior-posterior polarity is established at least 1 d ...
... The anterior-posterior axis of the mouse embryo becomes explicit morphologically at embryonic day (E)1 6.5, when the first mesoderm forms in the primitive streak region at the posterior side of the embryo. However, recent experiments show that anterior-posterior polarity is established at least 1 d ...
Platelet granule exocytosis: a comparison with chromaffin cells
... has also been described (Morgenstern et al., 1987). SNAREs are localized on platelet membranes in a manner to support fusion of granules with OCS, plasma membrane, or other granules (Feng et al., 2002). Despite the morphological differences between exocytosis in platelets and chromaffin cells, simil ...
... has also been described (Morgenstern et al., 1987). SNAREs are localized on platelet membranes in a manner to support fusion of granules with OCS, plasma membrane, or other granules (Feng et al., 2002). Despite the morphological differences between exocytosis in platelets and chromaffin cells, simil ...
Thrombopoietin expands hematopoietic stem cells after
... such as ADP, collagen, or thrombin (9, 10). Based on results using partially purified preparations of the hormone, previous reports concluded that TPO is a specific growth factor for the MK and platelet lineage (11, 12). However, several lines of evidence using recombinant protein now indicate that ...
... such as ADP, collagen, or thrombin (9, 10). Based on results using partially purified preparations of the hormone, previous reports concluded that TPO is a specific growth factor for the MK and platelet lineage (11, 12). However, several lines of evidence using recombinant protein now indicate that ...
Dynamics of the slowing segmentation clock reveal
... the basis of most somitogenesis models today (Lewis et al., 2009; Aulehla and Pourquié, 2010; Pourquié, 2011; Oates et al., 2012). Through experimental and theoretical data across a wide breadth of organisms, the periodicity and timing of somite formation is now thought to be controlled by a molecul ...
... the basis of most somitogenesis models today (Lewis et al., 2009; Aulehla and Pourquié, 2010; Pourquié, 2011; Oates et al., 2012). Through experimental and theoretical data across a wide breadth of organisms, the periodicity and timing of somite formation is now thought to be controlled by a molecul ...
Calcium Activation of ERK Mediated by Calmodulin Kinase I
... Ras/Rap family of small G proteins that in turn trigger a complex cascade of protein kinases terminating in activation of the MAP kinase family including the ERKs (17–20). The response of the ERK pathway to various stimuli can be cell type-specific and/or -dependent on regulation of different subcel ...
... Ras/Rap family of small G proteins that in turn trigger a complex cascade of protein kinases terminating in activation of the MAP kinase family including the ERKs (17–20). The response of the ERK pathway to various stimuli can be cell type-specific and/or -dependent on regulation of different subcel ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.