International Society for Biological Therapy of Cancer 23rd Annual
... of cells to the clinically relevant immunosuppressive drug mycophenolate mofetil (MMF, a prodrug of mycophenolic acid, MPA). Studies using Daudi and Sup-B15 human B-lineage tumor cells, OKT3stimulated peripheral blood mononuclear cell derived human T cells and established human T-cell lines demonstr ...
... of cells to the clinically relevant immunosuppressive drug mycophenolate mofetil (MMF, a prodrug of mycophenolic acid, MPA). Studies using Daudi and Sup-B15 human B-lineage tumor cells, OKT3stimulated peripheral blood mononuclear cell derived human T cells and established human T-cell lines demonstr ...
Disruption of Zebrafish Somite Development by Pharmacologic
... been demonstrated. Studies of Hsp90 function during vertebrate embryogenesis are complicated by several factors. These include the multigene nature of the vertebrate hsp90 family, including the presence of several pseudogenes (Gupta, 1995), and the fact that Hsp90 probably plays multiple roles at di ...
... been demonstrated. Studies of Hsp90 function during vertebrate embryogenesis are complicated by several factors. These include the multigene nature of the vertebrate hsp90 family, including the presence of several pseudogenes (Gupta, 1995), and the fact that Hsp90 probably plays multiple roles at di ...
Transcription Factor Positive Regulatory Domain 4 (PRDM4) recruits Protein Arginine
... added overnight at 4oC. The rest of the stainin g was as described above. Coverslips were mounted in DAKO mounting medium (DAKO). The following secondary antibodies were used : goat anti-mouse Alexa 488, goat anti-rabbit Alexa 568, goat anti-mouse Alexa 647 (Invitrogen, 1:1000), goat anti-rat Alexa ...
... added overnight at 4oC. The rest of the stainin g was as described above. Coverslips were mounted in DAKO mounting medium (DAKO). The following secondary antibodies were used : goat anti-mouse Alexa 488, goat anti-rabbit Alexa 568, goat anti-mouse Alexa 647 (Invitrogen, 1:1000), goat anti-rat Alexa ...
Transduction Cascade In Myeloid Cells: A Novel Cytokine Signal
... bcl-2 in B cells is dependent on this promoter element and phosphorylation of the associated trans-activating factor CREB, although participation of other upstream regulatory elements was also required. GM-CSF-induced CREB phosphorylation is independent of activation of the cAMP/GMP-dependent kinase ...
... bcl-2 in B cells is dependent on this promoter element and phosphorylation of the associated trans-activating factor CREB, although participation of other upstream regulatory elements was also required. GM-CSF-induced CREB phosphorylation is independent of activation of the cAMP/GMP-dependent kinase ...
the fine-tuning of the endomembrane system
... 2002; Cooper, 2007; Jones et al., 2007; Oldroyd et al., 2011; Bapaume & Reinhardt, 2012; Oldroyd, 2013). However the study of symbiosis is not limited to the tools of genetics. Symbiosis is based on the fine-tuning of host cell and bacteria that ensure the co-existence and mutual benefit. Such proce ...
... 2002; Cooper, 2007; Jones et al., 2007; Oldroyd et al., 2011; Bapaume & Reinhardt, 2012; Oldroyd, 2013). However the study of symbiosis is not limited to the tools of genetics. Symbiosis is based on the fine-tuning of host cell and bacteria that ensure the co-existence and mutual benefit. Such proce ...
Hormonal regulation of stem cell maintenance in roots
... but was instead associated with the loss of PIN auxin carrier accumulation (Lucas et al., 2011). Since PIN genes are not direct targets of SHR (Levesque et al., 2006), PIN protein abundance must be regulated by SHR at the post-transcriptional level (Lucas et al., 2011). It has been demonstrated th ...
... but was instead associated with the loss of PIN auxin carrier accumulation (Lucas et al., 2011). Since PIN genes are not direct targets of SHR (Levesque et al., 2006), PIN protein abundance must be regulated by SHR at the post-transcriptional level (Lucas et al., 2011). It has been demonstrated th ...
Amphibian gastrulation: history and evolution of a 125 year
... especially interested in embryological comparisons between different phyla, inasmuch as they shared similar embryonic stages. This was considered to be evidence for phylogenetic relationships between them. The early similarities between otherwise different animals were thus believed to prove their r ...
... especially interested in embryological comparisons between different phyla, inasmuch as they shared similar embryonic stages. This was considered to be evidence for phylogenetic relationships between them. The early similarities between otherwise different animals were thus believed to prove their r ...
Lipid-like materials for low-dose, in vivo gene silencing Please share
... 2001 (2), considerable effort has been directed towards their therapeutic application in humans (3). The most significant challenges to delivery include the relatively large size (∼13 kDa) and negative charge of siRNA molecules as well as their susceptibility to enzymatic degradation in vivo (4, 5). ...
... 2001 (2), considerable effort has been directed towards their therapeutic application in humans (3). The most significant challenges to delivery include the relatively large size (∼13 kDa) and negative charge of siRNA molecules as well as their susceptibility to enzymatic degradation in vivo (4, 5). ...
Coers, J, Bernstein-Hanley, I, Grotsky, D
... stained with a mouse mAb against the C. trachomatis major outer membrane protein (MOMP; Accurate Chemical and Scientific). For C. muridarum infections, cells were stained with a mouse mAb to Chlamydia LPS (RDI-Fitzgerald) or a mouse monoclonal anti-MOMP Ab specific for C. muridarum (29). An affinity ...
... stained with a mouse mAb against the C. trachomatis major outer membrane protein (MOMP; Accurate Chemical and Scientific). For C. muridarum infections, cells were stained with a mouse mAb to Chlamydia LPS (RDI-Fitzgerald) or a mouse monoclonal anti-MOMP Ab specific for C. muridarum (29). An affinity ...
Lead detected
... The distribution of lead in root tips of Dianthus carthusianorum was compared in populations from a zinc-lead waste heap in Bolesław near Olkusz and from a natural stand in the Botanical Garden in Lublin. The analyses were made at two developmental stages: seedlings (after 8 days of incubation in 5 ...
... The distribution of lead in root tips of Dianthus carthusianorum was compared in populations from a zinc-lead waste heap in Bolesław near Olkusz and from a natural stand in the Botanical Garden in Lublin. The analyses were made at two developmental stages: seedlings (after 8 days of incubation in 5 ...
Spindle pole body-anchored Kar3 drives the nucleus
... Kar1 of the yeast SPB. We show that this module underlies congression-specific MT organization. It anchors MT minus ends and, via Kar3 associated with the mating-specific Cik1 light chain, exerts the forces necessary for congression on MTs emanating from the SPB of the mating partner’s nucleus. This ...
... Kar1 of the yeast SPB. We show that this module underlies congression-specific MT organization. It anchors MT minus ends and, via Kar3 associated with the mating-specific Cik1 light chain, exerts the forces necessary for congression on MTs emanating from the SPB of the mating partner’s nucleus. This ...
Annals of Microbiology
... increased in size and the host cell wall lysed as a result, ending up as a very thin layer similar to a membrane (Fig. 3E). The intercellular material disappeared and the space was totally occupied by bacteria. Tomato cells adjacent to the invaded spaces appeared to lack contents and to be dead, whe ...
... increased in size and the host cell wall lysed as a result, ending up as a very thin layer similar to a membrane (Fig. 3E). The intercellular material disappeared and the space was totally occupied by bacteria. Tomato cells adjacent to the invaded spaces appeared to lack contents and to be dead, whe ...
In vitro and in vivo structure and activity relationship analysis of
... on angiogenesis remains unclear. Only two recent studies touched on the antiangiogenesis effect of polymethoxylated flavones and flavanones, focused mainly on nobiletin [20, 21]. It remain unclear that the relationship between chemical structural diversity of this subclass of flavonoids and mechanis ...
... on angiogenesis remains unclear. Only two recent studies touched on the antiangiogenesis effect of polymethoxylated flavones and flavanones, focused mainly on nobiletin [20, 21]. It remain unclear that the relationship between chemical structural diversity of this subclass of flavonoids and mechanis ...
Mechanisms of plant spindle formation
... When the nuclear envelope breaks down (preceding mitosis or meiosis), the compartmentalization is lost, but a gradient is formed: with RanGTP enriched in the vicinity of the chromosomes and RanGDP concentrated at the cell periphery. This gradient is thought to be critical for mitotic spindle assembl ...
... When the nuclear envelope breaks down (preceding mitosis or meiosis), the compartmentalization is lost, but a gradient is formed: with RanGTP enriched in the vicinity of the chromosomes and RanGDP concentrated at the cell periphery. This gradient is thought to be critical for mitotic spindle assembl ...
Signaling tip growth in plants Zhenbiao Yang
... tubes into bulbous tubes (H Li and Z Yang, unpublished data). These results indicate that Rop controls not only the establishment of cell polarity but also tip growth, as does CDC42 in tip-growing fission yeast [8]. The bulbous phenotype is analogous to tip swelling accompanied by non-localized intr ...
... tubes into bulbous tubes (H Li and Z Yang, unpublished data). These results indicate that Rop controls not only the establishment of cell polarity but also tip growth, as does CDC42 in tip-growing fission yeast [8]. The bulbous phenotype is analogous to tip swelling accompanied by non-localized intr ...
Spatial and temporal regulation of DNA
... degraded into sugars and other small molecules which can be readily taken up by the embryo. In this way, both scutellum and aleurone provide the machinery to obtain the required nutrients for the embryo. This process is controlled in time in such a way that the young plant can reach the stage of sel ...
... degraded into sugars and other small molecules which can be readily taken up by the embryo. In this way, both scutellum and aleurone provide the machinery to obtain the required nutrients for the embryo. This process is controlled in time in such a way that the young plant can reach the stage of sel ...
Mitogen-activated protein kinase kinase 1
... 2004), we used siRNA-mediated knockdown of expression. Using a siRNA duplex targeting the 3⬘ untranslated region, we observed ⬃95% knockdown of a stably expressed HAtagged version of MEK1 in HeLa cells by immunofluorescence (our unpublished data) and immunoblotting (Figure 1A). To confirm knockdown ...
... 2004), we used siRNA-mediated knockdown of expression. Using a siRNA duplex targeting the 3⬘ untranslated region, we observed ⬃95% knockdown of a stably expressed HAtagged version of MEK1 in HeLa cells by immunofluorescence (our unpublished data) and immunoblotting (Figure 1A). To confirm knockdown ...
THE EFFECT OF METAL BASED COMPLEXES ON THE IN VITRO.
... State, South Africa, has developed unique Rhodium - Ferrocene complexes that have not been previously tested in any biological system. New ferrocenecontaining betadiketones have been created from different ferrocenoic acids. The ...
... State, South Africa, has developed unique Rhodium - Ferrocene complexes that have not been previously tested in any biological system. New ferrocenecontaining betadiketones have been created from different ferrocenoic acids. The ...
The role of lymphatics in renal inflammation
... blood vascular system. It is composed of blunt-ended capillaries (30–80 μm in diameter) devoid of a basement membrane or pericytes (Figure 1, [5]). They consist of single-layered ‘oak leaf’-shaped partly overlapping cells, which are interconnected via discontinuously arranged ‘button-like’ junctions ...
... blood vascular system. It is composed of blunt-ended capillaries (30–80 μm in diameter) devoid of a basement membrane or pericytes (Figure 1, [5]). They consist of single-layered ‘oak leaf’-shaped partly overlapping cells, which are interconnected via discontinuously arranged ‘button-like’ junctions ...
Pathology of Genetically Engineered Mouse
... These models use a variety of approaches to target the expression of mutant or endogenous genes in specific cellular compartments. It should therefore not be surprising that a broad spectrum of pathologic changes develop in these models. Some of these changes histologically mimic human disease, wher ...
... These models use a variety of approaches to target the expression of mutant or endogenous genes in specific cellular compartments. It should therefore not be surprising that a broad spectrum of pathologic changes develop in these models. Some of these changes histologically mimic human disease, wher ...
Effects of cyclophosphamide treatment before
... After treatment during the preimplantation period they inspected rabbit embryos during organogenesis. When cyclophosphamide (CPA) (40 mg/kg intravenously) was given to pregnant rabbits before implantation, a high percentage of deformed fetuses was found on days 11, 17 and 30 p. c. (post-coitum), sug ...
... After treatment during the preimplantation period they inspected rabbit embryos during organogenesis. When cyclophosphamide (CPA) (40 mg/kg intravenously) was given to pregnant rabbits before implantation, a high percentage of deformed fetuses was found on days 11, 17 and 30 p. c. (post-coitum), sug ...
Identification of Pexl3p, a Peroxisomal Membrane Receptor for the
... netic approaches (for review see Rachubinski and Subramani, 1995). By analogy to the signal recognition- and targeting systems of other cellular membranes, binding of PTS1 and PTS2 to PTS1R and PTS2R is presumably followed by targeting of PTS1R and PTS2R to the peroxisomal membrane. The cognate pero ...
... netic approaches (for review see Rachubinski and Subramani, 1995). By analogy to the signal recognition- and targeting systems of other cellular membranes, binding of PTS1 and PTS2 to PTS1R and PTS2R is presumably followed by targeting of PTS1R and PTS2R to the peroxisomal membrane. The cognate pero ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.