Autophagy and pancreatitis
... conditions of intact vs. blocked lysosomal degradation, also indicate efficient basal autophagy in mouse pancreatic acinar cells (Jia et al., unpublished observations). One could speculate that because the exocrine pancreas has a very high rate of protein synthesis, it might have a greater need to r ...
... conditions of intact vs. blocked lysosomal degradation, also indicate efficient basal autophagy in mouse pancreatic acinar cells (Jia et al., unpublished observations). One could speculate that because the exocrine pancreas has a very high rate of protein synthesis, it might have a greater need to r ...
Optical mapping of atrioventricular node reveals a - AJP
... AV node preparation. New Zealand White rabbits (2.0–3.0 kg) were anesthetized with pentobarbital sodium (Nembutal; 35 mg/kg) by intravenous injection in an ear vein. The chest was opened, and heparin (200 U) was injected in the inferior vena cava. After a few minutes, the heart was removed and perfu ...
... AV node preparation. New Zealand White rabbits (2.0–3.0 kg) were anesthetized with pentobarbital sodium (Nembutal; 35 mg/kg) by intravenous injection in an ear vein. The chest was opened, and heparin (200 U) was injected in the inferior vena cava. After a few minutes, the heart was removed and perfu ...
Optical mapping of atrioventricular node reveals
... AV node preparation. New Zealand White rabbits (2.0–3.0 kg) were anesthetized with pentobarbital sodium (Nembutal; 35 mg/kg) by intravenous injection in an ear vein. The chest was opened, and heparin (200 U) was injected in the inferior vena cava. After a few minutes, the heart was removed and perfu ...
... AV node preparation. New Zealand White rabbits (2.0–3.0 kg) were anesthetized with pentobarbital sodium (Nembutal; 35 mg/kg) by intravenous injection in an ear vein. The chest was opened, and heparin (200 U) was injected in the inferior vena cava. After a few minutes, the heart was removed and perfu ...
Activation of ryanodine receptors induces calcium influx in a
... responses to thapsigargin, the most potent inhibitor of the family of intracellular Ca2+ pump proteins known as the SERCA (sarcoplasmic/endoplasmic reticulum calcium ATPase) enzymes [5,6]. Indeed, the most widely accepted pharmacological paradigm for activating Ca2+ influx pathways employs thapsigar ...
... responses to thapsigargin, the most potent inhibitor of the family of intracellular Ca2+ pump proteins known as the SERCA (sarcoplasmic/endoplasmic reticulum calcium ATPase) enzymes [5,6]. Indeed, the most widely accepted pharmacological paradigm for activating Ca2+ influx pathways employs thapsigar ...
Exocytosis acts as a modulator of the ILT4
... were expressed on neutrophils. The myelomonoblast PLB-985 cell line is considered as an appropriate model to generate neutrophil-like cells upon differentiation with Dimethylformamide (DMF) or Dimethylsulfoxide (DMSO) treatment. As shown in Fig. 1C, most of undifferentiated PLB-985 cells did not exp ...
... were expressed on neutrophils. The myelomonoblast PLB-985 cell line is considered as an appropriate model to generate neutrophil-like cells upon differentiation with Dimethylformamide (DMF) or Dimethylsulfoxide (DMSO) treatment. As shown in Fig. 1C, most of undifferentiated PLB-985 cells did not exp ...
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as
... characterized. A widely accepted model for ER-to-Golgi transport is based on the sequential action of COPII and COPI coat complexes. The COPII complex assembles by the ordered recruitment of cytosolic components on the ER membrane. Here, we have visualized two early components of the COPII machinery ...
... characterized. A widely accepted model for ER-to-Golgi transport is based on the sequential action of COPII and COPI coat complexes. The COPII complex assembles by the ordered recruitment of cytosolic components on the ER membrane. Here, we have visualized two early components of the COPII machinery ...
articles - Saleh Lab
... indicated that dsRNA is taken up by an active process involving receptor-mediated endocytosis. Pharmacological inhibition of endocytic pathways disrupted exogenous dsRNA entry and the induction of gene silencing. This dsRNA uptake mechanism seems to be evolutionarily conserved, as knockdown of ortho ...
... indicated that dsRNA is taken up by an active process involving receptor-mediated endocytosis. Pharmacological inhibition of endocytic pathways disrupted exogenous dsRNA entry and the induction of gene silencing. This dsRNA uptake mechanism seems to be evolutionarily conserved, as knockdown of ortho ...
PDF
... large amount of unglycosylated protein for structural studies, glycosylation-deficient mutants were generated in which Asn residues at positions 91, 94 and 99 were either replaced with Gln (Gly−), Asp (Gly− D), or all three Asn were deleted (Gly− ⌬). The biochemical properties of the mutant protein, ...
... large amount of unglycosylated protein for structural studies, glycosylation-deficient mutants were generated in which Asn residues at positions 91, 94 and 99 were either replaced with Gln (Gly−), Asp (Gly− D), or all three Asn were deleted (Gly− ⌬). The biochemical properties of the mutant protein, ...
Naturally Occurring Ligand Isoforms Receptor Binding and Function
... We used a comparative approach to identify the fetal liver tyrosine kinase 3 (flt3) ligand structure required for binding and function. Two conserved bovine flt3 ligand isoforms, which differ in a defined region within the extracellular domain, were identified and shown to be uniformly transcribed i ...
... We used a comparative approach to identify the fetal liver tyrosine kinase 3 (flt3) ligand structure required for binding and function. Two conserved bovine flt3 ligand isoforms, which differ in a defined region within the extracellular domain, were identified and shown to be uniformly transcribed i ...
Association of FMRP with Ribosomal Precursor Particles in the
... since in cells from fragile X patients, lacking FMRP, a normal transport of ribosomes from the nucleus to the cytoplasm occurs. On the other hand, the RNA binding properties of FMRP and the in vitro association with ribosomes led to different proposals for a theoretical function for FMRP. One possib ...
... since in cells from fragile X patients, lacking FMRP, a normal transport of ribosomes from the nucleus to the cytoplasm occurs. On the other hand, the RNA binding properties of FMRP and the in vitro association with ribosomes led to different proposals for a theoretical function for FMRP. One possib ...
refinement of an in vitro cell model for cytochrome p450 induction
... overlaid with ice-cold phosphate-buffered saline containing 5 mM EDTA (4 ml/plate). Cells and gel were then removed with a cell scraper, transferred to 15-ml tubes, and placed on ice for approximately 45 min to solubilize the Matrigel. Cells were resuspended by repeated pipetting until cells separat ...
... overlaid with ice-cold phosphate-buffered saline containing 5 mM EDTA (4 ml/plate). Cells and gel were then removed with a cell scraper, transferred to 15-ml tubes, and placed on ice for approximately 45 min to solubilize the Matrigel. Cells were resuspended by repeated pipetting until cells separat ...
Production and consumption of nitrous oxide in nitrate
... (Molstad et al., 2007). During this process, the medium was stirred at 950 r.p.m. to ensure sufficient gas exchange between the liquid and the gas phase. Excess pressure was relieved using a water-filled syringe. The vials were incubated at a constant temperature of 37 uC in a robotized incubation s ...
... (Molstad et al., 2007). During this process, the medium was stirred at 950 r.p.m. to ensure sufficient gas exchange between the liquid and the gas phase. Excess pressure was relieved using a water-filled syringe. The vials were incubated at a constant temperature of 37 uC in a robotized incubation s ...
Cooperative Regulation of Cell Polarity and Growth by Drosophila
... Cells in epithelial sheets are characterized by columnar or cuboidal shape, strong cell-cell adhesion, and pronounced apicobasal polarity. However, tumors of epithelial origin lose these characteristics as they progress from benign growth to malignant carcinoma, and this loss is associated with poor ...
... Cells in epithelial sheets are characterized by columnar or cuboidal shape, strong cell-cell adhesion, and pronounced apicobasal polarity. However, tumors of epithelial origin lose these characteristics as they progress from benign growth to malignant carcinoma, and this loss is associated with poor ...
Glycoproteins with Type Common and Type Specific Antigenic Sites
... i.e. cross reacting, and type specific antigenic sites involved in the interaction of virus with neutralizing antibody (Sim & Watson, t 973). These antigenic sites have been characterized further in that an antiserum has been raised in rabbits to the type common Band II antigen of an HSV I (HFEM) vi ...
... i.e. cross reacting, and type specific antigenic sites involved in the interaction of virus with neutralizing antibody (Sim & Watson, t 973). These antigenic sites have been characterized further in that an antiserum has been raised in rabbits to the type common Band II antigen of an HSV I (HFEM) vi ...
Nanoscale Architecture of Endoplasmic Reticulum Export Sites and
... The quality of a final electron tomogram is determined primarily by the quality of the initial specimen preservation. The membranous organelles of the secretory pathway are highly dynamic cellular structures that undergo changes in organization in the time range of seconds and even fractions of seco ...
... The quality of a final electron tomogram is determined primarily by the quality of the initial specimen preservation. The membranous organelles of the secretory pathway are highly dynamic cellular structures that undergo changes in organization in the time range of seconds and even fractions of seco ...
Specific Organization of Golgi Apparatus in Plant Cells
... Abstract—Microtubules, actin filaments, and Golgi apparatus are connected both directly and indirectly, but it is manifested differently depending on the cell organization and specialization, and these connections are considered in many original studies and reviews. In this review we would like to d ...
... Abstract—Microtubules, actin filaments, and Golgi apparatus are connected both directly and indirectly, but it is manifested differently depending on the cell organization and specialization, and these connections are considered in many original studies and reviews. In this review we would like to d ...
Interaction of Cationic Peptides with Lipoteichoic Acid and Gram
... Compounds with antiendotoxin properties have been extensively studied for their potential as therapeutic agents for sepsis attributable to gram-negative bacteria. However, with the increasing incidence of grampositive sepsis, there is interest in identifying compounds with a broad spectrum of action ...
... Compounds with antiendotoxin properties have been extensively studied for their potential as therapeutic agents for sepsis attributable to gram-negative bacteria. However, with the increasing incidence of grampositive sepsis, there is interest in identifying compounds with a broad spectrum of action ...
Autoregulation of Actin Synthesis by Physiological
... Modulation of osmotic effects by C2 toxin and phalloidin After primary culture for 18h, hepatocytes were incubated with normotonic or hypotonic medium with or without phalloidin or C2 toxin, respectively. Under normotonic conditions, phalloidin dramatically decreased cellular G-actin by 70% after in ...
... Modulation of osmotic effects by C2 toxin and phalloidin After primary culture for 18h, hepatocytes were incubated with normotonic or hypotonic medium with or without phalloidin or C2 toxin, respectively. Under normotonic conditions, phalloidin dramatically decreased cellular G-actin by 70% after in ...
Actin machinery: pushing the envelope Gary G Borisy* and Tatyana
... lamellipodial and filopodial protrusion. These sheet-like and spike-like processes, respectively, represent two different components of protrusive activity of crawling cells. Thus, the rocketing motion may be significant not only for understanding an apparently novel system of intracellular transpor ...
... lamellipodial and filopodial protrusion. These sheet-like and spike-like processes, respectively, represent two different components of protrusive activity of crawling cells. Thus, the rocketing motion may be significant not only for understanding an apparently novel system of intracellular transpor ...
pH-Sensitive Core-Shell Nanoparticles for Intracellular Drug Delivery
... 5.3.1 OVA Delivery in Dendritic Cells ..............................................................115 5.3.2 Functional Test of Cytosolic OVA Delivery: Priming of OVA-Specific CD8+ T Cells by Nanoparticle-treated DCs .......................................................118 5.4 Cytosolic Delivery ...
... 5.3.1 OVA Delivery in Dendritic Cells ..............................................................115 5.3.2 Functional Test of Cytosolic OVA Delivery: Priming of OVA-Specific CD8+ T Cells by Nanoparticle-treated DCs .......................................................118 5.4 Cytosolic Delivery ...
Isoprenoid biosynthesis in bacterial pathogens
... pathway leads to downregulation of primary metabolism genes, and upregulation of virulence factors and cell wall biosynthetic genes. The authors suggest that this dramatic shift in the transcriptome may be indicative of an altered metabolic state, where growth is halted and cells are primed for defe ...
... pathway leads to downregulation of primary metabolism genes, and upregulation of virulence factors and cell wall biosynthetic genes. The authors suggest that this dramatic shift in the transcriptome may be indicative of an altered metabolic state, where growth is halted and cells are primed for defe ...
A bi-functional siRNA construct induces RNA
... of the 32P-labelled probe will vary depending upon siRNA mismatch tolerance and also upon competing total cellular RNA sequences. In contrast, crook siRNA quantification is both direct and represents a constant for different siRNAs since detection is reliant upon the DNA component of the crook siRNA ...
... of the 32P-labelled probe will vary depending upon siRNA mismatch tolerance and also upon competing total cellular RNA sequences. In contrast, crook siRNA quantification is both direct and represents a constant for different siRNAs since detection is reliant upon the DNA component of the crook siRNA ...
TAL1occupancy_WuRevised_v16 - bx.psu.edu
... (Kassouf et al. 2008). These results show that direct binding to DNA is dispensable for some TAL1 functions in primitive erythropoiesis. Furthermore, a motif search on TAL1 binding sites in human proerythroblasts revealed that E-boxes are absent from over one-fifth of the sites. Indeed, GATA motifs ...
... (Kassouf et al. 2008). These results show that direct binding to DNA is dispensable for some TAL1 functions in primitive erythropoiesis. Furthermore, a motif search on TAL1 binding sites in human proerythroblasts revealed that E-boxes are absent from over one-fifth of the sites. Indeed, GATA motifs ...
Fetal Endoderm Primarily Holds the Temporal and Positional
... digestive enzyme activities in isografts of fetal intestinal implants, that the timing of intestinal development is primarily directed by an autonomous program (Ferguson et al., 1973; Kendall et al., 1979; Montgomery et al., 1981), Yet, modulation of this program may occur by extrinsic factors such ...
... digestive enzyme activities in isografts of fetal intestinal implants, that the timing of intestinal development is primarily directed by an autonomous program (Ferguson et al., 1973; Kendall et al., 1979; Montgomery et al., 1981), Yet, modulation of this program may occur by extrinsic factors such ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.