-Independent Manner Nucleus in a Cytokine
... have been implicated in the transcriptional activity of Stat1 and Stat3 (11). Stats exist as monomers in the cytoplasm before receptor activation (1). Cytokine stimulation leads to Jak activation followed by phosphorylation of tyrosine residues in the cytoplasmic part of the cytokine receptor. Phosp ...
... have been implicated in the transcriptional activity of Stat1 and Stat3 (11). Stats exist as monomers in the cytoplasm before receptor activation (1). Cytokine stimulation leads to Jak activation followed by phosphorylation of tyrosine residues in the cytoplasmic part of the cytokine receptor. Phosp ...
Modelling the structural response of an eukaryotic cell in the optical
... Analytic thick shell elastic model for actin cortex: Ample experimental evidence, albeit more indirect and qualitative, shows that out of the three cytoskeletal polymers, actin dominates the cell’s structural response5–8. Hence, in our first approach to model a suspended, spherical fibroblast in the ...
... Analytic thick shell elastic model for actin cortex: Ample experimental evidence, albeit more indirect and qualitative, shows that out of the three cytoskeletal polymers, actin dominates the cell’s structural response5–8. Hence, in our first approach to model a suspended, spherical fibroblast in the ...
CiC3-1a-Mediated Chemotaxis in the Deuterostome Invertebrate
... receptor molecule mediating the chemotactic effect is Gi protein coupled. Immunohistochemical analysis with anti-rCiC3-1aspecific Ab and in situ hybridization experiments with a riboprobe corresponding to the 3ⴕ-terminal sequence of CiC3-1, performed on tunic sections of LPS-injected animals, showed ...
... receptor molecule mediating the chemotactic effect is Gi protein coupled. Immunohistochemical analysis with anti-rCiC3-1aspecific Ab and in situ hybridization experiments with a riboprobe corresponding to the 3ⴕ-terminal sequence of CiC3-1, performed on tunic sections of LPS-injected animals, showed ...
PDF
... complements of progeny cells (Blair and Weisblat, 1982; Blair, 1983). However, cell fate may be less rigidly determined for epidermal cells; following ablation of one ectodermal teloblast (0, P or Q), progeny of the remaining teloblasts cover the territory of the body wall normally populated by prog ...
... complements of progeny cells (Blair and Weisblat, 1982; Blair, 1983). However, cell fate may be less rigidly determined for epidermal cells; following ablation of one ectodermal teloblast (0, P or Q), progeny of the remaining teloblasts cover the territory of the body wall normally populated by prog ...
Autocrine Effects of Fibroblast Growth Factor in Repair of Radiation
... cycle analysis by flow cytometry showed that 91 ±1.13% (mean ±SE of 6 experiments) of the cells were in G0-G| phase, 3.66% ±0.71% in S phase, and 5.33 ±0.66% in G2-M phase. Fig. 1 shows the dose-survival curves generated with these cells. Im mediately after irradiation the cells were seeded on e ...
... cycle analysis by flow cytometry showed that 91 ±1.13% (mean ±SE of 6 experiments) of the cells were in G0-G| phase, 3.66% ±0.71% in S phase, and 5.33 ±0.66% in G2-M phase. Fig. 1 shows the dose-survival curves generated with these cells. Im mediately after irradiation the cells were seeded on e ...
6 December 2009
... recent systematic study of the cleft width formed by HEK293 cells using thin sections prepared for transmission electron microscopy (TEM) by Wrobel et al. (2008) revealed that the cleft width formed between cells and a smooth silicon oxide substrate is not homogeneous, varying from tight attachment ...
... recent systematic study of the cleft width formed by HEK293 cells using thin sections prepared for transmission electron microscopy (TEM) by Wrobel et al. (2008) revealed that the cleft width formed between cells and a smooth silicon oxide substrate is not homogeneous, varying from tight attachment ...
Overexpression of a truncated cyclin B gene arrests Dictyostelium
... Total RNA was extracted from cells by the acid guanidinium thiocyanate method (Chomczynski and Sacchi, 1987). In order to reduce the hybridization background caused by the presence of the high copy number of the transgene in the transformants, the RNA preparations were resuspended in 500 µl of RES-1 ...
... Total RNA was extracted from cells by the acid guanidinium thiocyanate method (Chomczynski and Sacchi, 1987). In order to reduce the hybridization background caused by the presence of the high copy number of the transgene in the transformants, the RNA preparations were resuspended in 500 µl of RES-1 ...
Multiple Exocytotic Markers Accumulate at the Sites of Perifungal
... to a number of punctate cytoplasmic bodies (arrows), and a weaker signal along the plasma membrane (black arrowheads), fluorescence mainly accumulated along the newly laid cell walls (double arrow) of meristematic cells (A) and in the root hair tip (double arrows) of trichoblasts (B), indicating the ...
... to a number of punctate cytoplasmic bodies (arrows), and a weaker signal along the plasma membrane (black arrowheads), fluorescence mainly accumulated along the newly laid cell walls (double arrow) of meristematic cells (A) and in the root hair tip (double arrows) of trichoblasts (B), indicating the ...
The plant formin AtFH4 interacts with both actin and microtubules
... sedimented with microtubules, whereas AtFH4D6 remained in the supernatant. Fragment AtFH4D4 noticeably decreased the proportion of tubulin remaining in the supernatant (Fig. 2C; supplementary material Fig. S2). To test its bundling potential, AtFH4D4 was mixed with taxol-stabilised and Oregon-Greenl ...
... sedimented with microtubules, whereas AtFH4D6 remained in the supernatant. Fragment AtFH4D4 noticeably decreased the proportion of tubulin remaining in the supernatant (Fig. 2C; supplementary material Fig. S2). To test its bundling potential, AtFH4D4 was mixed with taxol-stabilised and Oregon-Greenl ...
NIH Public Access
... extrinsic signals are interpreted through an intrinsic mechanism that either commits the progenitor cell to the mitotic cell cycle or lead to exit from the cell cycle in order to differentiate. The endocrine cells that differentiate from progenitor cells are postmitotic, and direct lineage tracing a ...
... extrinsic signals are interpreted through an intrinsic mechanism that either commits the progenitor cell to the mitotic cell cycle or lead to exit from the cell cycle in order to differentiate. The endocrine cells that differentiate from progenitor cells are postmitotic, and direct lineage tracing a ...
Physcomitrella Cyclin-Dependent Kinase A Links
... and exhibit endoreduplication and thus resemble differentiated cells (Gaamouche et al., 2010). These results could be taken to mean that RBR and CDKA regulate both cell division and other cellular characteristics, but on the other hand, cellular characteristics could have been affected as a conseque ...
... and exhibit endoreduplication and thus resemble differentiated cells (Gaamouche et al., 2010). These results could be taken to mean that RBR and CDKA regulate both cell division and other cellular characteristics, but on the other hand, cellular characteristics could have been affected as a conseque ...
The ~53 Proto-Oncogene Can Act as a Suppressor of Transformation
... of the cellular retinoblastoma (Rb) gene (DeCaprio et al., 1988; Whyte et al., 1988). Deletion or inactivation of the Rb gene predisposes individuals to the development of human malignant retinoblastoma (Friend et al., 1986; Lee et al., 1987), and abnormalities in the Rb locus have recently been det ...
... of the cellular retinoblastoma (Rb) gene (DeCaprio et al., 1988; Whyte et al., 1988). Deletion or inactivation of the Rb gene predisposes individuals to the development of human malignant retinoblastoma (Friend et al., 1986; Lee et al., 1987), and abnormalities in the Rb locus have recently been det ...
Induction of the Stem-like Cell Regulator CD44
... In this article, we found that inhibitors of Rho-associated protein kinase (ROCK) or of actomyosin cytokinesis markedly facilitated the formation of spheroids from primary colon cancers, and revealed that the CD44high cells in the spheroids share common characteristics with CSCs. Unexpectedly, a fra ...
... In this article, we found that inhibitors of Rho-associated protein kinase (ROCK) or of actomyosin cytokinesis markedly facilitated the formation of spheroids from primary colon cancers, and revealed that the CD44high cells in the spheroids share common characteristics with CSCs. Unexpectedly, a fra ...
Human CHMP6, a myristoylated ESCRT-III protein, interacts directly
... [8–10]. At the endosome, ubiquitinated proteins are first recognized by Vps27, and are then transferred to recruited ESCRT-I (Vps23, Vps28 and Vps37) during MVB sorting [11,12]. ESCRT-I is then thought to activate functions of ESCRT-II (Vps22, Vps25 and Vps36) in the recruitment and oligomerization ...
... [8–10]. At the endosome, ubiquitinated proteins are first recognized by Vps27, and are then transferred to recruited ESCRT-I (Vps23, Vps28 and Vps37) during MVB sorting [11,12]. ESCRT-I is then thought to activate functions of ESCRT-II (Vps22, Vps25 and Vps36) in the recruitment and oligomerization ...
Mob4 plays a role in spindle focusing in Drosophila S2 cells
... centrosomes, as occurs in meiotic cells or in cells with disrupted centrosomes (Heald et al., 1996; Hinchcliffe et al., 2001; Khodjakov et al., 2000; Mahoney et al., 2006; Megraw et al., 2001). The process of spindle formation in somatic cells begins typically with the duplication and separation of ...
... centrosomes, as occurs in meiotic cells or in cells with disrupted centrosomes (Heald et al., 1996; Hinchcliffe et al., 2001; Khodjakov et al., 2000; Mahoney et al., 2006; Megraw et al., 2001). The process of spindle formation in somatic cells begins typically with the duplication and separation of ...
Inertly labeled monoclonal antibodies for PET and optical imaging
... Antibody-based therapy has become one of the most important and successful therapeutic modalities to treat cancer. The first application was just 20 years ago, but the concepts are much older. Antibody-based therapy is actually one of the oldest treatments for cancer patients. Back in 1895, two Fren ...
... Antibody-based therapy has become one of the most important and successful therapeutic modalities to treat cancer. The first application was just 20 years ago, but the concepts are much older. Antibody-based therapy is actually one of the oldest treatments for cancer patients. Back in 1895, two Fren ...
Modulating progenitor accumulation attenuates lung angiogenesis in a mouse model of asthma
... ABSTRACT: Asthmatic responses are associated with the lung homing of bone marrow (BM)derived progenitors implicated as effectors of disease pathology. Studies have shown that increases in lung extracted vascular endothelial progenitor cells (VEPCs) correlate with airway angiogenesis and declining lu ...
... ABSTRACT: Asthmatic responses are associated with the lung homing of bone marrow (BM)derived progenitors implicated as effectors of disease pathology. Studies have shown that increases in lung extracted vascular endothelial progenitor cells (VEPCs) correlate with airway angiogenesis and declining lu ...
Localization of Green Fluorescent Protein Fusions
... tobacco (Nicotiana tabacum) cells demonstrated that BP-80 and its homologs were predominantly concentrated on the lytic PVCs at steady state (Li et al., 2002). These results indicate that VSRs can be used as markers to define PVCs and that VSR proteins recycle back to Golgi briefly for cargo selecti ...
... tobacco (Nicotiana tabacum) cells demonstrated that BP-80 and its homologs were predominantly concentrated on the lytic PVCs at steady state (Li et al., 2002). These results indicate that VSRs can be used as markers to define PVCs and that VSR proteins recycle back to Golgi briefly for cargo selecti ...
Immunology Stack - University of Arizona
... (azoles). Other unique compounds are also targeted but these are the big hitters. ...
... (azoles). Other unique compounds are also targeted but these are the big hitters. ...
Restricted, canonical, stereotyped and convergent immunoglobulin
... that is, responses that induce essentially identical immunoglobulins from different mice, despite the massive potential diversity of VDJ rearrangement. For example, B-cell responses of C57BL/6 mice to NP select for canonical antibodies encoded by V186.2 recombined to DFI 16.1 D segment and the JH1 J ...
... that is, responses that induce essentially identical immunoglobulins from different mice, despite the massive potential diversity of VDJ rearrangement. For example, B-cell responses of C57BL/6 mice to NP select for canonical antibodies encoded by V186.2 recombined to DFI 16.1 D segment and the JH1 J ...
Augmin Plays a Critical Role in Organizing the
... to interact via the WD40 repeat protein NEDD1/GCP-WD (Zhu et al., 2008; Uehara et al., 2009). Among the eight subunits of the augmin complex, fly dim g-tubulin 4 (Dgt4) and human augminlike complex, subunit 8 (HAUS8)/HICE1 are MT-associated proteins (MAPs) and are likely responsible for establishing ...
... to interact via the WD40 repeat protein NEDD1/GCP-WD (Zhu et al., 2008; Uehara et al., 2009). Among the eight subunits of the augmin complex, fly dim g-tubulin 4 (Dgt4) and human augminlike complex, subunit 8 (HAUS8)/HICE1 are MT-associated proteins (MAPs) and are likely responsible for establishing ...
PDF
... cultures made no detectable difference to their outgrowth. Dermomyotomes were occasionally affected by the addition of avian transferrin. This tissue, which is of an epithelial nature, always maintained its integrity in the absence of transferrin, allowing very few individual cells to escape the epi ...
... cultures made no detectable difference to their outgrowth. Dermomyotomes were occasionally affected by the addition of avian transferrin. This tissue, which is of an epithelial nature, always maintained its integrity in the absence of transferrin, allowing very few individual cells to escape the epi ...
A Vacuolar Processing Enzyme, dVPE, Is Involved in Seed Coat
... disrupted organelles and cellular materials from the dead cells formed large aggregates. Cell layers that had lost their contents were compressed, resulting in folded cell walls (Figures 6C and 6D). Subsequently, cells in the ii3 layer started to shrink, so that the ii3 layer became compressed like ...
... disrupted organelles and cellular materials from the dead cells formed large aggregates. Cell layers that had lost their contents were compressed, resulting in folded cell walls (Figures 6C and 6D). Subsequently, cells in the ii3 layer started to shrink, so that the ii3 layer became compressed like ...
Mitochondrial alternative oxidase acts to dampen the generation of
... 1999, Parsons et al. 1999). This method was used to determine the rate of generation of H2O2 by wt and AS8 cells after 5 days growth in low P medium and during the period of stimulated respiration immediately following P resupply. After 5 days growth in low P medium, wt cells displayed a rate of H2O ...
... 1999, Parsons et al. 1999). This method was used to determine the rate of generation of H2O2 by wt and AS8 cells after 5 days growth in low P medium and during the period of stimulated respiration immediately following P resupply. After 5 days growth in low P medium, wt cells displayed a rate of H2O ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.