Comparison of test tube, gel column agglutination and solid
... taken from frozen stock which may have impacted results. However, the freeze/thaw manipulation of these samples did not appear to diminish reactivity in gel. With drug-treated cells, the findings obtained using IgG gel cards were comparable to the tube results, similar to the original work by Salama ...
... taken from frozen stock which may have impacted results. However, the freeze/thaw manipulation of these samples did not appear to diminish reactivity in gel. With drug-treated cells, the findings obtained using IgG gel cards were comparable to the tube results, similar to the original work by Salama ...
Annual Report 2014 The Kidney Project
... children’s health, and a faculty affiliate of the California Institute for Quantitative Biosciences (QB3). Dr. Roy earned a B.S. degree, magna cum laude, with general honors for triple majors in Physics, Mathematics (special honors), and Computer Science from Mount Union College in Alliance, Ohio. I ...
... children’s health, and a faculty affiliate of the California Institute for Quantitative Biosciences (QB3). Dr. Roy earned a B.S. degree, magna cum laude, with general honors for triple majors in Physics, Mathematics (special honors), and Computer Science from Mount Union College in Alliance, Ohio. I ...
Document
... iGenetics Regulation of cell division in normal cells Try iactivity in chapter 10 online – tracking down the cause of cancer ...
... iGenetics Regulation of cell division in normal cells Try iactivity in chapter 10 online – tracking down the cause of cancer ...
A New Model of the Human Atrial Myocyte with Variable T
... Atrial tissues were obtained from 16 consenting patients in sinus rhythm undergoing cardiac surgery. Procedures and experiments involving human atrial cells were approved by the West of Scotland Research Ethics Service (REC: 99MC002). Tissues were fixed in 2% paraformaldehyde then cryoprotected over ...
... Atrial tissues were obtained from 16 consenting patients in sinus rhythm undergoing cardiac surgery. Procedures and experiments involving human atrial cells were approved by the West of Scotland Research Ethics Service (REC: 99MC002). Tissues were fixed in 2% paraformaldehyde then cryoprotected over ...
7-2 Eukaryotic Cell Structure
... There are two types of ER—rough and smooth. The portion of the ER involved in protein synthesis is called rough endoplasmic reticulum, or rough ER. Ribosomes are found on the surface of rough ER. Rough ER is abundant in cells that produce large amounts of protein for export. ...
... There are two types of ER—rough and smooth. The portion of the ER involved in protein synthesis is called rough endoplasmic reticulum, or rough ER. Ribosomes are found on the surface of rough ER. Rough ER is abundant in cells that produce large amounts of protein for export. ...
The constant beat: cardiomyocytes adapt their forces by equal
... elasticity are still under debate since other studies indicate a complete loss of myofibrils on soft 1 kPa substrates (Bajaj et al., 2010), an elevated number of beating myocytes after maturation on very soft substrates (below 1 kPa) (Shapira-Schweitzer and Seliktar, 2007) or increasing force values ...
... elasticity are still under debate since other studies indicate a complete loss of myofibrils on soft 1 kPa substrates (Bajaj et al., 2010), an elevated number of beating myocytes after maturation on very soft substrates (below 1 kPa) (Shapira-Schweitzer and Seliktar, 2007) or increasing force values ...
Indication of use, mechanism of action, chemical structures
... Indication of use The potential applications of AONs are numerous because mRNA is ubiquitous and more readily accessible to manipulation than DNA. Currently, numerous AONs are being evaluated in clinical trials for treating cancer, inflammation (allergic, autoimmune and some other inflammatory disea ...
... Indication of use The potential applications of AONs are numerous because mRNA is ubiquitous and more readily accessible to manipulation than DNA. Currently, numerous AONs are being evaluated in clinical trials for treating cancer, inflammation (allergic, autoimmune and some other inflammatory disea ...
Microtubule cortical array organization and plant cell morphogenesis
... If microtubules are not generally polymerized into an ordered array [21–24], how is interphase organization created? The prevalent alternative theories have been that microtubules of diverse orientations and positions are selectively stabilized or that polymers are moved by motor activities from one ...
... If microtubules are not generally polymerized into an ordered array [21–24], how is interphase organization created? The prevalent alternative theories have been that microtubules of diverse orientations and positions are selectively stabilized or that polymers are moved by motor activities from one ...
Mediation of Clathrin-Dependent Trafficking during
... and division of the GMC facilitates the microscopy-based identification of nonlethal Arabidopsis mutants that disrupt cytokinesis, including cytokinesis defective1 (cyd1; Yang et al., 1999), stomatal cytokinesis defective1 (scd1; Falbel et al., 2003), and scd2 (see below). In the temperature-sensitiv ...
... and division of the GMC facilitates the microscopy-based identification of nonlethal Arabidopsis mutants that disrupt cytokinesis, including cytokinesis defective1 (cyd1; Yang et al., 1999), stomatal cytokinesis defective1 (scd1; Falbel et al., 2003), and scd2 (see below). In the temperature-sensitiv ...
The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
Cells are Either Prokaryotic or Eukaryotic
... between plant cells, the plasmodesmata, allow for the movement of materials among cells. Thanks to the plasmodesmata channels, the cytoplasm of one cell is continuous with the cytoplasm of the next; the plant as a whole can be thought of as having a single complement of continuous ...
... between plant cells, the plasmodesmata, allow for the movement of materials among cells. Thanks to the plasmodesmata channels, the cytoplasm of one cell is continuous with the cytoplasm of the next; the plant as a whole can be thought of as having a single complement of continuous ...
The posterior section of the chick`s area pellucida
... (1990) claimed that the posterior cellular contribution was derived from the germ wall margin (GWM), or as they called it “the deep portion of the marginal zone”. All these conflicting ideas were probably partly due to the fact that most of the above cited papers have based their conclusions on obse ...
... (1990) claimed that the posterior cellular contribution was derived from the germ wall margin (GWM), or as they called it “the deep portion of the marginal zone”. All these conflicting ideas were probably partly due to the fact that most of the above cited papers have based their conclusions on obse ...
Use of the BacTiter-Glo™ Microbial Cell Viability Assay to Study
... Similarly, RLU that result from the BacTiter-Glo™ Assay can be converted to bacterial cell numbers with a bacterial calibration curve. To obtain this curve, a bacterial suspension of an E. coli wild-type strain that had been grown for 38 hours without aeration in tryptone broth (TB; 1% tryptone, 0.5 ...
... Similarly, RLU that result from the BacTiter-Glo™ Assay can be converted to bacterial cell numbers with a bacterial calibration curve. To obtain this curve, a bacterial suspension of an E. coli wild-type strain that had been grown for 38 hours without aeration in tryptone broth (TB; 1% tryptone, 0.5 ...
Leukemia 2012 Paula
... gene that leads to CML is formed in some people and not in others. However, in a small number of patients, CML is caused by exposure to very high doses of radiation. This effect has been most carefully studied in the survivors of the atomicbomb blast in Japan; their risk of developing leukemia was s ...
... gene that leads to CML is formed in some people and not in others. However, in a small number of patients, CML is caused by exposure to very high doses of radiation. This effect has been most carefully studied in the survivors of the atomicbomb blast in Japan; their risk of developing leukemia was s ...
Lecture 25
... 1) Cells tuned for direction of motion (more broadly tuned than V1 cells. 2) Cells tuned for speed. (Some cells specifically tuned for speed. Not dependent on spatial frequency). 3) Large receptive field sizes. (Some are 100x bigger than V1 receptive fields). They range from 1-2deg in diameter in th ...
... 1) Cells tuned for direction of motion (more broadly tuned than V1 cells. 2) Cells tuned for speed. (Some cells specifically tuned for speed. Not dependent on spatial frequency). 3) Large receptive field sizes. (Some are 100x bigger than V1 receptive fields). They range from 1-2deg in diameter in th ...
Bases of clinical immunology
... 24. Children with a genetic deficiency of C6 have A. decreased resistance to viral infections B. increased hypersensitivity reactions C. increased frequency of cancer D. *decreased resistance to Neisseria bacteremia E. none of above 25. Cells - natural killer cells are A. B cells that can kill witho ...
... 24. Children with a genetic deficiency of C6 have A. decreased resistance to viral infections B. increased hypersensitivity reactions C. increased frequency of cancer D. *decreased resistance to Neisseria bacteremia E. none of above 25. Cells - natural killer cells are A. B cells that can kill witho ...
SepF, a novel FtsZ-interacting protein required for a late step in cell
... test whether the ylm mutant had increased sensitivity to FtsZ levels, the ylm deletion was introduced into cells in which ftsZ expression is under control of the IPTG-inducible Pspac promoter. Although low IPTG concentrations resulted in elongated cells, the presence of an ylm deletion did not subst ...
... test whether the ylm mutant had increased sensitivity to FtsZ levels, the ylm deletion was introduced into cells in which ftsZ expression is under control of the IPTG-inducible Pspac promoter. Although low IPTG concentrations resulted in elongated cells, the presence of an ylm deletion did not subst ...
Asymmetric Cell Divisions: Zygotes of Fucoid Algae as a
... pronucleus migrates to the egg pronucleus utilizing microtubules (Swope and Kropf 1993), and the zygote secretes a cell wall (Quatrano 1982) and an adhesive that attaches it firmly to the rock (Hable and Kropf 1998). Once attached, the young zygote monitors its environment for positional information. ...
... pronucleus migrates to the egg pronucleus utilizing microtubules (Swope and Kropf 1993), and the zygote secretes a cell wall (Quatrano 1982) and an adhesive that attaches it firmly to the rock (Hable and Kropf 1998). Once attached, the young zygote monitors its environment for positional information. ...
Cytoplasmic streaming enables the distribution of molecules
... yield Pe∼100. Of course, for the transport of larger species, with correspondingly smaller diffusion constants, Pe will be even larger (Goldstein et al. 2008; van de Meent et al. 2008). In this flow-dominated regime, the transport and mixing of molecular species can be very complex, involving the fo ...
... yield Pe∼100. Of course, for the transport of larger species, with correspondingly smaller diffusion constants, Pe will be even larger (Goldstein et al. 2008; van de Meent et al. 2008). In this flow-dominated regime, the transport and mixing of molecular species can be very complex, involving the fo ...
Hydrogen peroxide-induced changes in intracellular pH of
... of guard cells challenged with H2O2 (Fig 4 and Fig 5), which are coincident with the epidermal bioassay (Fig 2 and Fig 3). These results imply that the alkalinization of the cytosol may be accomplished via a H+ efflux from cytosol to vacuole through vacuolar H+-ATPase. Proton gradient across tonopla ...
... of guard cells challenged with H2O2 (Fig 4 and Fig 5), which are coincident with the epidermal bioassay (Fig 2 and Fig 3). These results imply that the alkalinization of the cytosol may be accomplished via a H+ efflux from cytosol to vacuole through vacuolar H+-ATPase. Proton gradient across tonopla ...
Cytoplasmic streaming enables the distribution of molecules
... yield Pe∼100. Of course, for the transport of larger species, with correspondingly smaller diffusion constants, Pe will be even larger (Goldstein et al. 2008; van de Meent et al. 2008). In this flow-dominated regime, the transport and mixing of molecular species can be very complex, involving the fo ...
... yield Pe∼100. Of course, for the transport of larger species, with correspondingly smaller diffusion constants, Pe will be even larger (Goldstein et al. 2008; van de Meent et al. 2008). In this flow-dominated regime, the transport and mixing of molecular species can be very complex, involving the fo ...
PDF
... Growing buds of 3-4 mm in length were extirpated from the parental animal. They were incubated in Millipore-filtered sea water (MFSW) containing 0.1 ^jg, 0.3 ^g, 1/igor lO^gml" 1 of TC-14 and/or 5mM galactose, and were allowed to develop for two days in the presence of streptomycin (0.5xlO- 4 gUmr') ...
... Growing buds of 3-4 mm in length were extirpated from the parental animal. They were incubated in Millipore-filtered sea water (MFSW) containing 0.1 ^jg, 0.3 ^g, 1/igor lO^gml" 1 of TC-14 and/or 5mM galactose, and were allowed to develop for two days in the presence of streptomycin (0.5xlO- 4 gUmr') ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.