HAUSP, a deubiquitinating enzyme for p53, is polyubiquitinated
... regions between nucleosomes. As expected, rHAUSP-transfected HeLa cells died completely. We observed extracted genomic DNA from apoptotic HeLa cells induced by rHAUSP and rHAUSP (C224S), visualized as DNA ladders, indicating that these cells are apoptotic (data not shown). We also investigated the e ...
... regions between nucleosomes. As expected, rHAUSP-transfected HeLa cells died completely. We observed extracted genomic DNA from apoptotic HeLa cells induced by rHAUSP and rHAUSP (C224S), visualized as DNA ladders, indicating that these cells are apoptotic (data not shown). We also investigated the e ...
Drug-Induced Aplastic Anaemia and Agranulocytosis Incidence and
... through committedstt:m cells, into erythropoiesis, granulomonocytopoiesis,megakaryocytopoiesisand B-celllymphopoiesis. The system is under the control of humoral regulators, of which the best-defined is erythropoietin (Fried 1979;Stohlman 1968), and short-range cellular regulators, including particu ...
... through committedstt:m cells, into erythropoiesis, granulomonocytopoiesis,megakaryocytopoiesisand B-celllymphopoiesis. The system is under the control of humoral regulators, of which the best-defined is erythropoietin (Fried 1979;Stohlman 1968), and short-range cellular regulators, including particu ...
PDF
... cells, we utilized the signal sequence trap (SST) method, which can efficiently isolate genes encoding a protein with a signal sequence (Kojima and Kitamura, 1999; Watanabe et al., 2007). As there are several protocols for generating oval cells in rats and mice, the characteristics of oval cells mig ...
... cells, we utilized the signal sequence trap (SST) method, which can efficiently isolate genes encoding a protein with a signal sequence (Kojima and Kitamura, 1999; Watanabe et al., 2007). As there are several protocols for generating oval cells in rats and mice, the characteristics of oval cells mig ...
Phosphorylation of eIF2α in response to 26S proteasome inhibition
... 2 or 4 h profoundly decreased protein synthesis [9]. Similarly, Mimnaugh et al. [10] have also shown that the proteasome inhibitor, lactacystin, decreased the synthesis of most cellular proteins, while it specifically induced the synthesis of stress proteins Hsp72 (heat-shock protein 72) and Hsp90 i ...
... 2 or 4 h profoundly decreased protein synthesis [9]. Similarly, Mimnaugh et al. [10] have also shown that the proteasome inhibitor, lactacystin, decreased the synthesis of most cellular proteins, while it specifically induced the synthesis of stress proteins Hsp72 (heat-shock protein 72) and Hsp90 i ...
The Arabidopsis Exocyst Complex Is Involved in
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
RNA SHAPE analysis in living cells
... RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2′-hydroxyl acylation analyzed by primer extension (SHA ...
... RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2′-hydroxyl acylation analyzed by primer extension (SHA ...
Expression of a Nondegradable Cyclin B1 Affects
... (A) and (C) Appearance of 20-d-old seedlings germinated on plant agar deriving from lines CycBMut6-I. C, cotyledon; L, leaf. (B), (D), and (E) Environmental scanning electron micrographs showing abnormalities in cotyledon of weakly affected offsprings from line CycB1Mut6-I (B) and in the epidermal c ...
... (A) and (C) Appearance of 20-d-old seedlings germinated on plant agar deriving from lines CycBMut6-I. C, cotyledon; L, leaf. (B), (D), and (E) Environmental scanning electron micrographs showing abnormalities in cotyledon of weakly affected offsprings from line CycB1Mut6-I (B) and in the epidermal c ...
Direct Engagement of Inhibitory Fc Receptor Microbial Immune
... forms of Fc␥RII (Fc␥RIIA and Fc␥RIIB, products of two separate genes). Fc␥RI, Fc␥RIIA, and Fc␥RIII are activating receptors associated with ITAM (15), whereas Fc␥RIIB is an inhibitory receptor that has an ITIM in its cytoplasmic tail (16). Many immunosuppressive signals described for Fc␥RIIB such as ...
... forms of Fc␥RII (Fc␥RIIA and Fc␥RIIB, products of two separate genes). Fc␥RI, Fc␥RIIA, and Fc␥RIII are activating receptors associated with ITAM (15), whereas Fc␥RIIB is an inhibitory receptor that has an ITIM in its cytoplasmic tail (16). Many immunosuppressive signals described for Fc␥RIIB such as ...
Ex vivo processing for maturation of Arabidopsis
... RcCysEP is synthesized as a pre-pro-enzyme and is co-translationally transferred into the ER, where the presequence is removed. The pro-enzyme is transported from the ER to the cytoplasm of the senescing cells in the form of ER-derived ribosome studded ricinosomes. The final stage of PCD is characte ...
... RcCysEP is synthesized as a pre-pro-enzyme and is co-translationally transferred into the ER, where the presequence is removed. The pro-enzyme is transported from the ER to the cytoplasm of the senescing cells in the form of ER-derived ribosome studded ricinosomes. The final stage of PCD is characte ...
MCDB 4650 Class 19 Patterning of the Limb
... Of the following, which experiment best tests the hypothesis that cells in the limb bud have a pre-specified fate? a. Remove AER, see what cells types are made b. Mark distal cells in an early limb bud and see if they become distal cells in the limb c. Remove factors required for proliferation; see ...
... Of the following, which experiment best tests the hypothesis that cells in the limb bud have a pre-specified fate? a. Remove AER, see what cells types are made b. Mark distal cells in an early limb bud and see if they become distal cells in the limb c. Remove factors required for proliferation; see ...
IDEXX Urine Sediment Guide
... How to perform a dry prep/line smear Performing a dry prep or line smear is an extremely cost-effective means of confirming the presence or absence of bacteria, of differentiating between cocci and short rods, and for characterizing various cellular elements in the urine sample. ...
... How to perform a dry prep/line smear Performing a dry prep or line smear is an extremely cost-effective means of confirming the presence or absence of bacteria, of differentiating between cocci and short rods, and for characterizing various cellular elements in the urine sample. ...
Phloem loading and unloading - HAL
... dyes into the mesophyll cells has shown in several herbaceous species that the dye actually entered the veins but gave no clear demonstration of dye entry into the companion cell-sieve tube complex itself. The data presented above shows that osmotic gradients may be found between cells connected by ...
... dyes into the mesophyll cells has shown in several herbaceous species that the dye actually entered the veins but gave no clear demonstration of dye entry into the companion cell-sieve tube complex itself. The data presented above shows that osmotic gradients may be found between cells connected by ...
CycD1, a Putative G1 Cyclin from Antirrhinum majus
... using soluble GFP under the CaMV35S promoter gave strong fluorescence in both cytosol and nuclei (Figures 1C and 1F). We were unable to find any mitotic cells containing a detectable GFP:CycD1 in our transient experiments, so to study the dynamics of localization during the cell cycle, we made stabl ...
... using soluble GFP under the CaMV35S promoter gave strong fluorescence in both cytosol and nuclei (Figures 1C and 1F). We were unable to find any mitotic cells containing a detectable GFP:CycD1 in our transient experiments, so to study the dynamics of localization during the cell cycle, we made stabl ...
CycD1, a Putative G1 Cyclin from Antirrhinum majus, Accelerates
... using soluble GFP under the CaMV35S promoter gave strong fluorescence in both cytosol and nuclei (Figures 1C and 1F). We were unable to find any mitotic cells containing a detectable GFP:CycD1 in our transient experiments, so to study the dynamics of localization during the cell cycle, we made stabl ...
... using soluble GFP under the CaMV35S promoter gave strong fluorescence in both cytosol and nuclei (Figures 1C and 1F). We were unable to find any mitotic cells containing a detectable GFP:CycD1 in our transient experiments, so to study the dynamics of localization during the cell cycle, we made stabl ...
Interferon-α (IFN-α) suppresses HTLV-1 gene
... Background: Human T-cell leukemia virus type-1 (HTLV-1) is the causative retrovirus of adult T-cell leukemia/ lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1 gene expression is maintained at low levels in vivo by unknown mechanisms. A combination therap ...
... Background: Human T-cell leukemia virus type-1 (HTLV-1) is the causative retrovirus of adult T-cell leukemia/ lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1 gene expression is maintained at low levels in vivo by unknown mechanisms. A combination therap ...
molecular mechanisms of mechanoperception in plants
... protein complexes that provide a pathway for the movement of ions from one side of the membrane to the other. Ligand- and voltage-gated ion channels have been studied for many years in plant systems, most notably in guard cell signal transduction (reviewed in Hedrich, 2012; Ward et al., 2009). Thoug ...
... protein complexes that provide a pathway for the movement of ions from one side of the membrane to the other. Ligand- and voltage-gated ion channels have been studied for many years in plant systems, most notably in guard cell signal transduction (reviewed in Hedrich, 2012; Ward et al., 2009). Thoug ...
REST/NRSF TARGET GENES IN NEURONAL AND BETA CELLS
... among the thousands of putative targets found within each whole genome.20 A comparative analysis of the NRSEs between species using a profile-based approach has refined the number of NRSE sites.12 Thus, 895 NRSE sites conserved in human, mouse, rat, and dog (with an estimated false-positive rate of ...
... among the thousands of putative targets found within each whole genome.20 A comparative analysis of the NRSEs between species using a profile-based approach has refined the number of NRSE sites.12 Thus, 895 NRSE sites conserved in human, mouse, rat, and dog (with an estimated false-positive rate of ...
The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
Fulltext PDF - Indian Academy of Sciences
... Characterization of the ppGpp0 strain showed it to filament upon nutritional down shift, suggesting a role for (p)ppGpp in the process of cell division (Xiao et al. 1991). Insights of its role in cell division came from the studies carried out in the D’Ari lab to understand the function of PBP2 (pen ...
... Characterization of the ppGpp0 strain showed it to filament upon nutritional down shift, suggesting a role for (p)ppGpp in the process of cell division (Xiao et al. 1991). Insights of its role in cell division came from the studies carried out in the D’Ari lab to understand the function of PBP2 (pen ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.