Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast.
... Microtubules are cytoskeletal structures that function in eukaryotes to segregate chromosomes during cell division, position organelles, organize the cytoplasm, and provide form and motility to cilia and flagella. A central property of microtubules is that they are dynamic; at steady state, individu ...
... Microtubules are cytoskeletal structures that function in eukaryotes to segregate chromosomes during cell division, position organelles, organize the cytoplasm, and provide form and motility to cilia and flagella. A central property of microtubules is that they are dynamic; at steady state, individu ...
Cell wall synthesis is necessary for membrane dynamics during
... peptide capable of polymerizing could serve a similar role. Bulge formation in these mutants therefore offers a useful system to examine candidate force-generating mechanisms responsible for membrane movements in bacteria. Most bacteria contain peptidoglycan, a rigid polymer built from disaccharide ...
... peptide capable of polymerizing could serve a similar role. Bulge formation in these mutants therefore offers a useful system to examine candidate force-generating mechanisms responsible for membrane movements in bacteria. Most bacteria contain peptidoglycan, a rigid polymer built from disaccharide ...
Preface The plant cell cycle in context
... these core regulators are now revealing more of the interplay with plant specific factors (Blomme et al., 2014, this volume). Cloning and sequence analysis thus showed that plants (and Arabidopsis is used here as the example) contain two main types of CDK involved in primary control of the mitotic c ...
... these core regulators are now revealing more of the interplay with plant specific factors (Blomme et al., 2014, this volume). Cloning and sequence analysis thus showed that plants (and Arabidopsis is used here as the example) contain two main types of CDK involved in primary control of the mitotic c ...
Mitochondrial alternative oxidase acts to dampen the generation of
... 1999, Parsons et al. 1999). This method was used to determine the rate of generation of H2O2 by wt and AS8 cells after 5 days growth in low P medium and during the period of stimulated respiration immediately following P resupply. After 5 days growth in low P medium, wt cells displayed a rate of H2O ...
... 1999, Parsons et al. 1999). This method was used to determine the rate of generation of H2O2 by wt and AS8 cells after 5 days growth in low P medium and during the period of stimulated respiration immediately following P resupply. After 5 days growth in low P medium, wt cells displayed a rate of H2O ...
Hypersensitivity of Cockayne`s Syndrome Cells
... going high rates of transcription. These areas of the genome have also been shown to repair UV-induced DNA damage at a much faster rate than nontranscribed or inactive genes. Though as yet there is no evidence that Topo I or Topo II is directly involved in nucleotide excision repair, it is possible ...
... going high rates of transcription. These areas of the genome have also been shown to repair UV-induced DNA damage at a much faster rate than nontranscribed or inactive genes. Though as yet there is no evidence that Topo I or Topo II is directly involved in nucleotide excision repair, it is possible ...
Hemodynamic disorders p.1 - Patho
... 11 Kidney infarct 11a Early phase of the myocardial infarct 12 Lung infarct 10 Cancer cell embolism 290 Thrombus ...
... 11 Kidney infarct 11a Early phase of the myocardial infarct 12 Lung infarct 10 Cancer cell embolism 290 Thrombus ...
Amniotic membrane modulates innate immune response inhibiting
... Upon ligand binding, dsRNA receptors activate by one hand interferon-regulating factors-3 and -7 (IRF-3 and -7), which promote transcription of type I interferons (IFN) such as IFN-a and IFN- ...
... Upon ligand binding, dsRNA receptors activate by one hand interferon-regulating factors-3 and -7 (IRF-3 and -7), which promote transcription of type I interferons (IFN) such as IFN-a and IFN- ...
Repairing the Damaged Plasma Membrane of the
... parts to a mitochondrion. They are: the outer mitochondrial membrane the intermembrane space (the space between the outer and inner membranes) the inner mitochondrial membrane the cristae space (formed by infoldings of the inner membrane) the matrix (space within the inner membrane) The mitochondria ...
... parts to a mitochondrion. They are: the outer mitochondrial membrane the intermembrane space (the space between the outer and inner membranes) the inner mitochondrial membrane the cristae space (formed by infoldings of the inner membrane) the matrix (space within the inner membrane) The mitochondria ...
1 L-Leucine improves the anemia and
... rps19 morphants raised in egg water showed a profound anemia compared to control morphants (Figure 1 B,F,J compared to D,H and L). rps19 morphants treated with 100mM LLeucine showed a striking improvement in hemoglobinization at 4 dpf compared to those raised in egg water alone (Figure 1E, I, M comp ...
... rps19 morphants raised in egg water showed a profound anemia compared to control morphants (Figure 1 B,F,J compared to D,H and L). rps19 morphants treated with 100mM LLeucine showed a striking improvement in hemoglobinization at 4 dpf compared to those raised in egg water alone (Figure 1E, I, M comp ...
Inhibition of L-Fucose Incorporation into
... dryness under vacuum at 30°,redissolved in 1 ml of water, and centrifuged at 33,000 x g for 20 min to remove ethanolsoluble but water-insoluble material. Descending Whatman No. 3MM paper chromatography was carried out with 95% ethanol:1.0 M ammonium acetate (7:3, v/v) for 9 hr. Airdried paper strip ...
... dryness under vacuum at 30°,redissolved in 1 ml of water, and centrifuged at 33,000 x g for 20 min to remove ethanolsoluble but water-insoluble material. Descending Whatman No. 3MM paper chromatography was carried out with 95% ethanol:1.0 M ammonium acetate (7:3, v/v) for 9 hr. Airdried paper strip ...
TR Worksheets
... Xylem vessels are hollow—their end walls break to form a tube for easy flow of water and dissolved mineral ions. Vessels can be up to several metres long and are strengthened with lignin (in spiral, ring or net patterns), making them impermeable to water and giving them support. Pits in the walls en ...
... Xylem vessels are hollow—their end walls break to form a tube for easy flow of water and dissolved mineral ions. Vessels can be up to several metres long and are strengthened with lignin (in spiral, ring or net patterns), making them impermeable to water and giving them support. Pits in the walls en ...
Cell cycle: Checkpoint proteins and kinetochores
... timing of a normal cell cycle? Taylor and McKeon [7] investigated this question by examining the effect of overexpressing the mBub1 amino-terminal domain on the timing of the cell cycle. They found that the overexpression had no effect on the timing of G1, S or G2 phases of the cell cycle, but delay ...
... timing of a normal cell cycle? Taylor and McKeon [7] investigated this question by examining the effect of overexpressing the mBub1 amino-terminal domain on the timing of the cell cycle. They found that the overexpression had no effect on the timing of G1, S or G2 phases of the cell cycle, but delay ...
PDF - The Journal of Immunology
... presented at elevated levels and in an inflammatory milieu (5, 6). Glucosylceramide (GlcCer) with a 24:1 N-acyl chain paired with a d18:1 sphingosine base is one such endogenous CD1d-presented iNKT cell Ag (7, 8). Expression of this endogenous glycolipid Ag can be increased by innate pattern recogni ...
... presented at elevated levels and in an inflammatory milieu (5, 6). Glucosylceramide (GlcCer) with a 24:1 N-acyl chain paired with a d18:1 sphingosine base is one such endogenous CD1d-presented iNKT cell Ag (7, 8). Expression of this endogenous glycolipid Ag can be increased by innate pattern recogni ...
Maturing reticulocytes internalize plasma membrane
... state of therapeutic erythrocyte products generated ex vivo from diverse stem cell sources.9-11 In the present study, we developed and characterized a robust ex vivo culture system that replicates human erythropoiesis using peripheral blood CD34⫹ cells isolated from the mononuclear cell fraction dis ...
... state of therapeutic erythrocyte products generated ex vivo from diverse stem cell sources.9-11 In the present study, we developed and characterized a robust ex vivo culture system that replicates human erythropoiesis using peripheral blood CD34⫹ cells isolated from the mononuclear cell fraction dis ...
Roles of vaccinia virus EEV-specific proteins in intracellular actin tail
... syncytium formation after low pH shock was examined (Fig. 6). BS-C-1 cells infected with WR, ∆A34R or ∆A36R developed the usual cytopathic effect and did not fuse if incubated at pH 7 (Fig. 6 a–c). However, if these cultures were briefly exposed to pH 5, cells infected with WR and ∆A36R fused (Fig. ...
... syncytium formation after low pH shock was examined (Fig. 6). BS-C-1 cells infected with WR, ∆A34R or ∆A36R developed the usual cytopathic effect and did not fuse if incubated at pH 7 (Fig. 6 a–c). However, if these cultures were briefly exposed to pH 5, cells infected with WR and ∆A36R fused (Fig. ...
Developmental neurobiology of hydra, a model animal of cnidarians1
... Introduction to the nerve net of hydra: unique features of the diffuse nervous system The freshwater coelenterate hydra has a simple body plan. Like all coelenterates, it is a diploblastic animal: it is composed of two layers of epithelial cells, the endoderm and the ectoderm. The apical end is the ...
... Introduction to the nerve net of hydra: unique features of the diffuse nervous system The freshwater coelenterate hydra has a simple body plan. Like all coelenterates, it is a diploblastic animal: it is composed of two layers of epithelial cells, the endoderm and the ectoderm. The apical end is the ...
The Relationship between Intracellular Free Iron and Cell Injury in
... ([Ca 2⫹]i), but early studies established its sensitivity to other ions (Grynkiewicz et al., 1985); e.g., fura-2 is very sensitive to Zn 2⫹ and can be used to monitor small changes in [Zn 2⫹]i in neurons (Cheng and Reynolds, 1998). Cations, such as Ca 2⫹ and Zn 2⫹, alter the excitation spectrum of f ...
... ([Ca 2⫹]i), but early studies established its sensitivity to other ions (Grynkiewicz et al., 1985); e.g., fura-2 is very sensitive to Zn 2⫹ and can be used to monitor small changes in [Zn 2⫹]i in neurons (Cheng and Reynolds, 1998). Cations, such as Ca 2⫹ and Zn 2⫹, alter the excitation spectrum of f ...
The membrane form of the DNA repair protein Ku interacts at the cell
... tumor cells (Prabhakar et al, 1990; Dalziel et al, 1992; Ginis et al, 1995; Ginis and Faller, 2000; Lynch et al, 2001). Furthermore, the localization of Ku in membrane lipid rafts of mammalian cells has been recently shown (Lucero et al, 2003). The expression of Ku outside of the nucleus highly sugg ...
... tumor cells (Prabhakar et al, 1990; Dalziel et al, 1992; Ginis et al, 1995; Ginis and Faller, 2000; Lynch et al, 2001). Furthermore, the localization of Ku in membrane lipid rafts of mammalian cells has been recently shown (Lucero et al, 2003). The expression of Ku outside of the nucleus highly sugg ...
PDF
... the lateral ventricle of P1 mice, most of the ependymal cells were immature and had non-polarized, short cilia (Fig. 3A), but by P7 the ependymal cells were mature and bore long, polarized cilia (Fig. 3B). To determine whether ciliary beating in immature and mature ependymal cells correlated with ba ...
... the lateral ventricle of P1 mice, most of the ependymal cells were immature and had non-polarized, short cilia (Fig. 3A), but by P7 the ependymal cells were mature and bore long, polarized cilia (Fig. 3B). To determine whether ciliary beating in immature and mature ependymal cells correlated with ba ...
The transcription factor Th-POK negatively regulates
... ceramide (␣GalCer), a potent agonist for their semi-invariant TCR. Similar populations of ␣GalCer-CD1d–reactive T lymphocytes are found in other mammalian species, and in fact, iNKT cells from humans and mice can cross-react with ␣GalCer presented by the opposite species, demonstrating a high degree ...
... ceramide (␣GalCer), a potent agonist for their semi-invariant TCR. Similar populations of ␣GalCer-CD1d–reactive T lymphocytes are found in other mammalian species, and in fact, iNKT cells from humans and mice can cross-react with ␣GalCer presented by the opposite species, demonstrating a high degree ...
Probes for Multiplexed Detection of GFP
... tools for investigating respiration, mitosis, apoptosis, multidrug resistance, substrate degradation and detoxification, intracellular transport and sorting and more. Moreover, unlike antibodies, these fluorescent probes can be used to investigate organelle structure and activity in live cells with ...
... tools for investigating respiration, mitosis, apoptosis, multidrug resistance, substrate degradation and detoxification, intracellular transport and sorting and more. Moreover, unlike antibodies, these fluorescent probes can be used to investigate organelle structure and activity in live cells with ...
The cell cycle and pluripotency
... Geminin (HUGO approved symbol GMNN) is down-regulated in trophoblast giant cells and the ablation of geminin is sufficient to cause commitment of pluripotent inner cell mass cells to the trophoblast lineage [33]. In addition to its role in preventing endoreplication, geminin is present in G1 in mESC ...
... Geminin (HUGO approved symbol GMNN) is down-regulated in trophoblast giant cells and the ablation of geminin is sufficient to cause commitment of pluripotent inner cell mass cells to the trophoblast lineage [33]. In addition to its role in preventing endoreplication, geminin is present in G1 in mESC ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.