DNA asymmetry in stem cells – immortal or mortal?
... the various techniques used to address this question, and discuss their strengths and limitations. In the majority of studies, nucleotide analogs such as 5-bromo29-deoxyuridine (BrdU) have been used to label and distinguish two sister chromatids. In a pulse–chase experiment (Fig. 1A), nucleotide ana ...
... the various techniques used to address this question, and discuss their strengths and limitations. In the majority of studies, nucleotide analogs such as 5-bromo29-deoxyuridine (BrdU) have been used to label and distinguish two sister chromatids. In a pulse–chase experiment (Fig. 1A), nucleotide ana ...
PERSPECTIVE
... the animal morphogen Dpp meets this criterion6,7. Until recently, however, conducting an equivalent experiment for auxin was impossible, as we lacked key knowledge about the factors that synthesize, and function downstream of, this phytohormone. Therefore, there is little evidence to date that would ...
... the animal morphogen Dpp meets this criterion6,7. Until recently, however, conducting an equivalent experiment for auxin was impossible, as we lacked key knowledge about the factors that synthesize, and function downstream of, this phytohormone. Therefore, there is little evidence to date that would ...
Organelle Project - WLPCS Upper School
... Organelle Frayer Model Directions: Create a Frayer model of a cell organelle. Use past work and Sec 7.3 in textbook as resources A colored, labeled drawing of the organelle and description of the structure general location in the cell. (Ex. The nucleus is generally in the ...
... Organelle Frayer Model Directions: Create a Frayer model of a cell organelle. Use past work and Sec 7.3 in textbook as resources A colored, labeled drawing of the organelle and description of the structure general location in the cell. (Ex. The nucleus is generally in the ...
Bridging the divide between cytokinesis and cell
... underlying cellular membrane. The plant primary cell wall is a complex and heavily cross-linked polysaccharide made up of crystalline cellulose microfibrils within a matrix of hemicelluloses and pectins [23]. Regulated pectin cross-linking functions to allow cellulose microfibril separation during c ...
... underlying cellular membrane. The plant primary cell wall is a complex and heavily cross-linked polysaccharide made up of crystalline cellulose microfibrils within a matrix of hemicelluloses and pectins [23]. Regulated pectin cross-linking functions to allow cellulose microfibril separation during c ...
Cell Structure Jepordy
... The chemical workbench of the cell. A system of canals that transports substances into the cell. Smooth and Rough. ...
... The chemical workbench of the cell. A system of canals that transports substances into the cell. Smooth and Rough. ...
Transfer of Primordial Germ-cells inXenopus laevis
... The experiments described above demonstrate that grafts of posterior endoderm containing the so-called primordial germ-cells can be successfully carried out between embryos of Xenopus at the neurula stage. The healing process is facilitated if the ecto-mesodermal coat of the ventral endoderm is tran ...
... The experiments described above demonstrate that grafts of posterior endoderm containing the so-called primordial germ-cells can be successfully carried out between embryos of Xenopus at the neurula stage. The healing process is facilitated if the ecto-mesodermal coat of the ventral endoderm is tran ...
Interleukin-6 Gene Expression in Multiple Myeloma: A Characteristic
... mature plasma cell morphology and function. Whereas the recognizable tumor cells in myeloma are the most mature B cells, early lymphoid cells are involved in the disease and probably represent the proliferative, preplasma cells compartment.'-' The apparent involvement of a common early progenitor in ...
... mature plasma cell morphology and function. Whereas the recognizable tumor cells in myeloma are the most mature B cells, early lymphoid cells are involved in the disease and probably represent the proliferative, preplasma cells compartment.'-' The apparent involvement of a common early progenitor in ...
Theory and Algorithms for Constructing Discrete Morse
... smooth functions we can also take the intersection of stable and unstable manifolds; the resulting partition is called the Morse-Smale complex [12]. Several issues must be resolved when translating the above picture to a computational setting: adapting Morse theory to non-smooth functions and manifo ...
... smooth functions we can also take the intersection of stable and unstable manifolds; the resulting partition is called the Morse-Smale complex [12]. Several issues must be resolved when translating the above picture to a computational setting: adapting Morse theory to non-smooth functions and manifo ...
Document
... In 1985, it was shown that it is possible to obtain precursors to many different blood cells from mouse embryonic stem cells [9]. Perkins was able to obtain all the major lineages of progenitor cells from mouse embryoid bodies, even without adding hematopoietic growth factors [45]. Mouse embryonic s ...
... In 1985, it was shown that it is possible to obtain precursors to many different blood cells from mouse embryonic stem cells [9]. Perkins was able to obtain all the major lineages of progenitor cells from mouse embryoid bodies, even without adding hematopoietic growth factors [45]. Mouse embryonic s ...
Agrin is required for survival and function of
... and inflammation,1,2 as well as in leukocyte adhesion and motility.3 Agrin has been extensively studied in the context of the neuromuscular junction (NMJ), where the protein exerts a key role as regulator of postsynaptic differentiation.4 However, although agrin is broadly expressed during developme ...
... and inflammation,1,2 as well as in leukocyte adhesion and motility.3 Agrin has been extensively studied in the context of the neuromuscular junction (NMJ), where the protein exerts a key role as regulator of postsynaptic differentiation.4 However, although agrin is broadly expressed during developme ...
The MHC I immunopeptidome conveys to the cell surface an
... the indicated proteins were determined by western blotting. b-Actin served as a loading control. Data are representative of three independent experiments. (B) EL4 cells were treated or not (ctrl) with rapamycin for 48 h. One thousand cells were counted for each condition. Cell size was measured by l ...
... the indicated proteins were determined by western blotting. b-Actin served as a loading control. Data are representative of three independent experiments. (B) EL4 cells were treated or not (ctrl) with rapamycin for 48 h. One thousand cells were counted for each condition. Cell size was measured by l ...
Uptake of glutamate, not glutamine synthetase, regulates adaptation
... Glutamine synthetase activity during cell growth Changes in GS activity have been associated with the concentration of glutamine in the medium and these were investigated. The intracellular glutamine synthetase activity of McCoy (Fig. 3A) and MDCK cells (Fig. 3B) was determined by colorimetric assay ...
... Glutamine synthetase activity during cell growth Changes in GS activity have been associated with the concentration of glutamine in the medium and these were investigated. The intracellular glutamine synthetase activity of McCoy (Fig. 3A) and MDCK cells (Fig. 3B) was determined by colorimetric assay ...
Graphene Oxide for Biomedical Applications
... rGO was synthesized by sonication and followed by reduction of PEGlayted GO sheets. Micron and nano sized (lateral) rGO sheets were obtained and tested for cyto and geno toxic effects. A high concentration of 100 mg/mL after 1 h exposure time, cytotoxic effect was observed and this study showed the ...
... rGO was synthesized by sonication and followed by reduction of PEGlayted GO sheets. Micron and nano sized (lateral) rGO sheets were obtained and tested for cyto and geno toxic effects. A high concentration of 100 mg/mL after 1 h exposure time, cytotoxic effect was observed and this study showed the ...
No-Needle Mesotherapy™ lIpo KoNtoUr
... recontouring. Treatment protocols are based on non-invasive transdermal penetration utilizing dual wavelength laser and three proprietary electrical waveforms. The therapy protocols are designed to impact lipolytic processes in sub-dermal fat ...
... recontouring. Treatment protocols are based on non-invasive transdermal penetration utilizing dual wavelength laser and three proprietary electrical waveforms. The therapy protocols are designed to impact lipolytic processes in sub-dermal fat ...
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... allele for bam as described previously (McKearin and Ohlstein, 1995; Ohlstein et al., 2000). bamBG is a strong allele for bam as described previously (Chen and McKearin, 2005). piwiEP and P{hs-gal4} have been described previously (Chen and McKearin, 2005; Cox et al., 2000) Ago1k08121 has been descri ...
... allele for bam as described previously (McKearin and Ohlstein, 1995; Ohlstein et al., 2000). bamBG is a strong allele for bam as described previously (Chen and McKearin, 2005). piwiEP and P{hs-gal4} have been described previously (Chen and McKearin, 2005; Cox et al., 2000) Ago1k08121 has been descri ...
MODULE 1 dental № TOPIC TEST 1. Histology as object
... At the patient with a dry pleuritis noise of friction of pleura is listened. At what defeat of type of epithelia this symptom is marked? A. Stratified epithelium B. Simple squamous* C. Transitional D. Cuboidal E. Cilindrical In the experiment the structure of contact between epithelial cells affecte ...
... At the patient with a dry pleuritis noise of friction of pleura is listened. At what defeat of type of epithelia this symptom is marked? A. Stratified epithelium B. Simple squamous* C. Transitional D. Cuboidal E. Cilindrical In the experiment the structure of contact between epithelial cells affecte ...
Targets of Oncogenes and Tumor Suppressors: Key for
... inhibitor, p27KIP1 [23-26]. Mechanisms of Myc activation by growth factors are poorly understood. It is suggested that this activation may be realized via Ras-independent signaling pathways activated by Src oncoprotein and via RasRafMAP kinase cascades inducing activation of Ets1 and/or E2F (the p ...
... inhibitor, p27KIP1 [23-26]. Mechanisms of Myc activation by growth factors are poorly understood. It is suggested that this activation may be realized via Ras-independent signaling pathways activated by Src oncoprotein and via RasRafMAP kinase cascades inducing activation of Ets1 and/or E2F (the p ...
Histochemical Polymorphism of Keratin Pearls in Squamous Cell
... In compliance with the objectives of the study a complex of histological and histochemical methods has been applied during the investigation. The analysis has been carried out on post operational material, taken from 25 patients with keratinizing squamous cell carcinoma of lung. The obtained materia ...
... In compliance with the objectives of the study a complex of histological and histochemical methods has been applied during the investigation. The analysis has been carried out on post operational material, taken from 25 patients with keratinizing squamous cell carcinoma of lung. The obtained materia ...
GFP-tagging of cell components reveals the dynamics of subcellular
... appressorium-like swellings were preferentially formed over the anticlinal walls of plant epidermal cells. A finger-like structure is indicated by the arrowhead. (c) Germ tubes growing across the surface of the cotyledon. Insert at the top right is the region indicated by the square in the main pane ...
... appressorium-like swellings were preferentially formed over the anticlinal walls of plant epidermal cells. A finger-like structure is indicated by the arrowhead. (c) Germ tubes growing across the surface of the cotyledon. Insert at the top right is the region indicated by the square in the main pane ...
- ISpatula
... *if the apical buds (the primary source of auxin) are removed, the inhibition of axillary buds is removed. *Mutants that overproduce cytokinins or plant treated with cytokinins tend to be bushier than normal . *The polar flow of auxin down the shoot trigger the synthesis of Strigolactones which repr ...
... *if the apical buds (the primary source of auxin) are removed, the inhibition of axillary buds is removed. *Mutants that overproduce cytokinins or plant treated with cytokinins tend to be bushier than normal . *The polar flow of auxin down the shoot trigger the synthesis of Strigolactones which repr ...
Human Corneal Endothelial Cells Expanded In Vitro Are a Powerful
... Obstacle for in Vitro Growth of HCECs Contact-inhibition is present in non-transformed normal cells when neighboring cells are in contact with one another. This fact causes low regenerative capability of in vivo human corneal endothelial cells. The conventional approach to grow HCECs in vitro is to ...
... Obstacle for in Vitro Growth of HCECs Contact-inhibition is present in non-transformed normal cells when neighboring cells are in contact with one another. This fact causes low regenerative capability of in vivo human corneal endothelial cells. The conventional approach to grow HCECs in vitro is to ...
doxorubicin and paclitaxel cause different changes in plasma
... combination. Thus, in this study we aimed at investigating the effect of anticancer drugs doxorubicin (DOX) and paclitaxel (PTX), alone or in combination, on the properties of the plasma membrane of MCF-7 human breast cancer cells. Both drugs are extensively used in clinical practice in therapy of a ...
... combination. Thus, in this study we aimed at investigating the effect of anticancer drugs doxorubicin (DOX) and paclitaxel (PTX), alone or in combination, on the properties of the plasma membrane of MCF-7 human breast cancer cells. Both drugs are extensively used in clinical practice in therapy of a ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.