pdf - John Innes Centre
... bounded by rigid cell walls, it is often found that the properties of the plant extracellular matrix play a critical role in the process of tissue and cell morphogenesis. This principle is vividly illustrated by the example of the rhizobium–legume symbiosis. Remodelling of the plant cell wall forms ...
... bounded by rigid cell walls, it is often found that the properties of the plant extracellular matrix play a critical role in the process of tissue and cell morphogenesis. This principle is vividly illustrated by the example of the rhizobium–legume symbiosis. Remodelling of the plant cell wall forms ...
Bradycardic and Proarrhythmic Properties of Sinus Node Inhibitors
... in HEK293 cells, and If, recorded from isolated mouse sinoatrial node cells. Duration of activation/deactivation depends on HCN subtype as described under Materials and Methods. B, example of use-dependent block of cloned HCN channels, shown for HCN4 in the presence of three concentrations of cilobr ...
... in HEK293 cells, and If, recorded from isolated mouse sinoatrial node cells. Duration of activation/deactivation depends on HCN subtype as described under Materials and Methods. B, example of use-dependent block of cloned HCN channels, shown for HCN4 in the presence of three concentrations of cilobr ...
Active Stable Maintenance Functions in Low Copy
... necessary for the activity of the post-segregational killing system (Zielenkiewicz and Ceglowski, 2005). The ε and ζ genes can also be expressed from the weak Pε promoter which is not subject to Omega regulation (Sitkiewicz, PhD Thesis, 2002). The activity of the Pε promoter may explain the observa ...
... necessary for the activity of the post-segregational killing system (Zielenkiewicz and Ceglowski, 2005). The ε and ζ genes can also be expressed from the weak Pε promoter which is not subject to Omega regulation (Sitkiewicz, PhD Thesis, 2002). The activity of the Pε promoter may explain the observa ...
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... Labor Zheren Zhang, Dennis Claessen and Daniel E. Rozen * Institute of Biology, Leiden University, Leiden, Netherlands ...
... Labor Zheren Zhang, Dennis Claessen and Daniel E. Rozen * Institute of Biology, Leiden University, Leiden, Netherlands ...
Station 1: Components of prokaryotic cells
... are plenty of good bacteria but these don’t make the news so often because they just get on with their jobs and don’t cause any trouble. In fact, without good bacteria we would have a lot of trouble surviving because they manage to keep you safe from a lot of the bad bacteria! Although they are smal ...
... are plenty of good bacteria but these don’t make the news so often because they just get on with their jobs and don’t cause any trouble. In fact, without good bacteria we would have a lot of trouble surviving because they manage to keep you safe from a lot of the bad bacteria! Although they are smal ...
Arachidonic acid inhibits the store-operated Ca2+ current in rat liver
... Under conditions used in the experiments with H4IIE cells described above (Figure 1), AA did not seem to activate any specific conductance. However, in other cell types, AA has been shown to activate highly selective Ca2+ channels with properties distinct from that of the SOCs in those cells [16]. O ...
... Under conditions used in the experiments with H4IIE cells described above (Figure 1), AA did not seem to activate any specific conductance. However, in other cell types, AA has been shown to activate highly selective Ca2+ channels with properties distinct from that of the SOCs in those cells [16]. O ...
Altered Cell Fate in LiCI-Treated Sea Urchin Embryos
... are small and abnormally shaped. The two smaller spheres are attached to the larger one by a ring of constricted cells and together appear to be a greatly enlarged gut. The smallest sphere often contains an invaginated structure. When embryos are exposed to 60 mM LiCl, they undergo cleavage which re ...
... are small and abnormally shaped. The two smaller spheres are attached to the larger one by a ring of constricted cells and together appear to be a greatly enlarged gut. The smallest sphere often contains an invaginated structure. When embryos are exposed to 60 mM LiCl, they undergo cleavage which re ...
Downloadable Full Text - DSpace@MIT
... gave rise to chimeras that transmitted the mutant allele. To generate UTX-null animals, conditional mutant males with the germ-line active Nestin-cre allele (see experimental procedures) were crossed with UtxKO/Wt females to generate a 1:1:1:1 expected ratio of Utxwt and UtxKO males and UtxKO/Wt and ...
... gave rise to chimeras that transmitted the mutant allele. To generate UTX-null animals, conditional mutant males with the germ-line active Nestin-cre allele (see experimental procedures) were crossed with UtxKO/Wt females to generate a 1:1:1:1 expected ratio of Utxwt and UtxKO males and UtxKO/Wt and ...
Membrane trafficking and polar growth in root hairs and pollen tubes
... in root hair elongation (Wen et al., 2005), and in Arabidopsis thaliana sec8 mutants display defects in pollen tube initiation and growth (Cole et al., 2005). Rth1/Sec3 and AtSec8 are orthologues of two subunits of the exocyst complex that, in yeast and animal cells, guides polarized exocytosis. Int ...
... in root hair elongation (Wen et al., 2005), and in Arabidopsis thaliana sec8 mutants display defects in pollen tube initiation and growth (Cole et al., 2005). Rth1/Sec3 and AtSec8 are orthologues of two subunits of the exocyst complex that, in yeast and animal cells, guides polarized exocytosis. Int ...
Cellular and Subcellular Localization of Peroxidase
... Im Neuenheimer Feld 364, D-6900 Heidelberg, Bundes republik Deutschland. Reprint requests to Prof. Dr. M. Wink. Verlag der Zeitschrift für Naturforschung, D-7400 Tübingen ...
... Im Neuenheimer Feld 364, D-6900 Heidelberg, Bundes republik Deutschland. Reprint requests to Prof. Dr. M. Wink. Verlag der Zeitschrift für Naturforschung, D-7400 Tübingen ...
- PlantingScience
... Premise: Many students know that plants require water to live, but fewer understand the processes by which plants take up, transport, and release water. Although plants and plant organs clearly have ...
... Premise: Many students know that plants require water to live, but fewer understand the processes by which plants take up, transport, and release water. Although plants and plant organs clearly have ...
Rapid movement of axonal neurofilaments interrupted by prolonged
... Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of ...
... Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of ...
Planar cell polarity signalling regulates cell adhesion properties in
... performed using 1 nN contact force, 10 m/second approach and retraction velocity, and contact times of 5 and 10 seconds. The order of these two contact time conditions was varied randomly for each pair of cells to prevent systematic bias or history effects. Each condition was repeated up to three t ...
... performed using 1 nN contact force, 10 m/second approach and retraction velocity, and contact times of 5 and 10 seconds. The order of these two contact time conditions was varied randomly for each pair of cells to prevent systematic bias or history effects. Each condition was repeated up to three t ...
Histology-Based Screen for Zebrafish Mutants with Abnormal Cell
... males, which were outcrossed with homozygous goldenb1 (gol) females to generate F1 progeny for screening. Early pressure parthenogenesis (EP) was used to generate half-tetrads from F1 females. At 7 days of age, half-tetrad larvae were fixed in 10% neutral buffered formalin, and up to 64 sibs from ind ...
... males, which were outcrossed with homozygous goldenb1 (gol) females to generate F1 progeny for screening. Early pressure parthenogenesis (EP) was used to generate half-tetrads from F1 females. At 7 days of age, half-tetrad larvae were fixed in 10% neutral buffered formalin, and up to 64 sibs from ind ...
Bacterial Outer Membrane Vesicles as a Delivery System for
... the parent bacteria but are not capable of replication, have been a fascinating subject in vaccine development studies. For instance, Neisseria meningitidis OMVs stimulate a strong immune response and have been developed into a vaccine formula, Bexsero (Novartis), which was approved in Europe. Howev ...
... the parent bacteria but are not capable of replication, have been a fascinating subject in vaccine development studies. For instance, Neisseria meningitidis OMVs stimulate a strong immune response and have been developed into a vaccine formula, Bexsero (Novartis), which was approved in Europe. Howev ...
digitalis - Circulation
... glycoside levels which produce positive inotropism without contracture it has been possible to demonstrate clearly that the exchangeability or "turnover" of Ca+ + is increased, but it is more difficult to validate a net gain of Ca+ + by the cell.27 28 With sensitive technics based on the ability to ...
... glycoside levels which produce positive inotropism without contracture it has been possible to demonstrate clearly that the exchangeability or "turnover" of Ca+ + is increased, but it is more difficult to validate a net gain of Ca+ + by the cell.27 28 With sensitive technics based on the ability to ...
Extra-embryonic endoderm differentiation
... a thick BM that acts as a barrier between maternal and embryonic environments, thus forming the parietal yolk sac (Salamat et al., 1995). The mechanisms that regulate differentiation and behaviour of the extra-embryonic endoderm cells are only now becoming apparent. VE cell differentiation has been ...
... a thick BM that acts as a barrier between maternal and embryonic environments, thus forming the parietal yolk sac (Salamat et al., 1995). The mechanisms that regulate differentiation and behaviour of the extra-embryonic endoderm cells are only now becoming apparent. VE cell differentiation has been ...
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... Cbl-b−/− mice demonstrate robust T cell and NK cell-mediated antitumor immunity. As a result of these murine studies, Cbl-b is considered a potential target for therapeutic manipulation in human cancer immunotherapy. The PD-L1/PD-1 pathway of immune regulation is presently an important therapeutic f ...
... Cbl-b−/− mice demonstrate robust T cell and NK cell-mediated antitumor immunity. As a result of these murine studies, Cbl-b is considered a potential target for therapeutic manipulation in human cancer immunotherapy. The PD-L1/PD-1 pathway of immune regulation is presently an important therapeutic f ...
A Cytoplasm to Vacuole Targeting Pathway in P. pastoris
... We analyzed the maturation status and localization in growth conditions of PpApe1‑CFP in the absence of P. pastoris orthologs of proteins involved in all autophagy‑related pathways (PpAtg5, PpAtg8, PpAtg9, and PpVps15), or in the absence of two proteins (PpAtg11 and PpVac8) required only for the Cvt ...
... We analyzed the maturation status and localization in growth conditions of PpApe1‑CFP in the absence of P. pastoris orthologs of proteins involved in all autophagy‑related pathways (PpAtg5, PpAtg8, PpAtg9, and PpVps15), or in the absence of two proteins (PpAtg11 and PpVac8) required only for the Cvt ...
Expression Sequences and Distribution of Two Primary Cell
... adhesion molecule (L-CAM) were examined in Xenopus laevis embryos by immunohistochemical and biochemical techniques. Because of the technical difficulties presented by the existence of multiple polypeptide forms of CAMs and by autofluorescence of yolkcontaining cells, special care was taken in choos ...
... adhesion molecule (L-CAM) were examined in Xenopus laevis embryos by immunohistochemical and biochemical techniques. Because of the technical difficulties presented by the existence of multiple polypeptide forms of CAMs and by autofluorescence of yolkcontaining cells, special care was taken in choos ...
CD133 CXCR4 colon cancer cells exhibit metastatic potential and predict poor prognosis
... The comparison of the incidence of tumor/metastasis formation by in vivo mouse models was performed with Fisher’s exact test. For analyzing the association between CD133+CXCR4+ cell content and various clinical factors, the ‘age’ as a continuous variable was expressed as mean (SD) and comparison bet ...
... The comparison of the incidence of tumor/metastasis formation by in vivo mouse models was performed with Fisher’s exact test. For analyzing the association between CD133+CXCR4+ cell content and various clinical factors, the ‘age’ as a continuous variable was expressed as mean (SD) and comparison bet ...
Apoptosis-inducing factor is involved in the regulation of caspase
... in a rapid loss of neuronal survival beginning at 12 h, and within 24 h, survival had decreased to 20% (Fig. 1 A). In contrast, Apaf1-deficient neurons remained largely viable during the first 24 h of treatment. However, after 24 h, cell survival began to decline and by 48 h, 30% of Apaf1/ neur ...
... in a rapid loss of neuronal survival beginning at 12 h, and within 24 h, survival had decreased to 20% (Fig. 1 A). In contrast, Apaf1-deficient neurons remained largely viable during the first 24 h of treatment. However, after 24 h, cell survival began to decline and by 48 h, 30% of Apaf1/ neur ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.