pdf-Dokument - Universität Bonn
... membrane proteins into the ER disrupting retrograde trafficking, as shown using brefeldin-A (BFA), which is a protein trafficking inhibitor. This suggests that ARF1GAP play a regulator role towards ARF during Golgi apparatus to ER transport. ARFGAPs are generally considered a group of proteins, whic ...
... membrane proteins into the ER disrupting retrograde trafficking, as shown using brefeldin-A (BFA), which is a protein trafficking inhibitor. This suggests that ARF1GAP play a regulator role towards ARF during Golgi apparatus to ER transport. ARFGAPs are generally considered a group of proteins, whic ...
Dual mode of paraxial mesoderm formation during chick gastrulation
... mesoderm. In double- (Fig. 1d) or single-graft (Fig. 1 i and l) experiments analyzed after 24 h, cells from the 90% level of the streak mostly gave rise to cells in medial somites from the anterior level of the somitic region to the tail bud (SI Table 1; Fig. 1 e, g, and j). In contrast, grafts of m ...
... mesoderm. In double- (Fig. 1d) or single-graft (Fig. 1 i and l) experiments analyzed after 24 h, cells from the 90% level of the streak mostly gave rise to cells in medial somites from the anterior level of the somitic region to the tail bud (SI Table 1; Fig. 1 e, g, and j). In contrast, grafts of m ...
Ultrastructural Observations on Lumbar Spinal Cord
... Eight individuals with transected lumbar spinal cord, previously utilized for the analysis of behavioral recovery and histology at 29, 36 and 45 post-lesion were also utilized for the present electron microscopic study (Alibardi, 2010, 2014). The progressive time-points were selected to detect possi ...
... Eight individuals with transected lumbar spinal cord, previously utilized for the analysis of behavioral recovery and histology at 29, 36 and 45 post-lesion were also utilized for the present electron microscopic study (Alibardi, 2010, 2014). The progressive time-points were selected to detect possi ...
Interaction of the Two Frontal Eye Fields Before Saccade Onset
... individual records. For inhibition, the time of the last spike before interspike interval increase was averaged. For excitation, the time of the first spike after interspike interval decrease was averaged. The averages thus calculated corresponded to the values suggested by histograms (such as in Fi ...
... individual records. For inhibition, the time of the last spike before interspike interval increase was averaged. For excitation, the time of the first spike after interspike interval decrease was averaged. The averages thus calculated corresponded to the values suggested by histograms (such as in Fi ...
The Dynamic Changes of Tonoplasts in Guard
... requirement for the rapid change of their volume. Here we report that the complex vacuolar membrane system may play an important role in the rapid increase of vacuolar volume in the guard cells during stomatal movement in Vicia faba. Small vacuoles fused rapidly with each other to form big ones in V ...
... requirement for the rapid change of their volume. Here we report that the complex vacuolar membrane system may play an important role in the rapid increase of vacuolar volume in the guard cells during stomatal movement in Vicia faba. Small vacuoles fused rapidly with each other to form big ones in V ...
Graft Rejection following Burn Injury Mechanism for Effective
... with 18 U.S.C. Section 1734 solely to indicate this fact. ...
... with 18 U.S.C. Section 1734 solely to indicate this fact. ...
Chapter # PLASMA MEMBRANE PHOSPHOLIPID ASYMMETRY
... from an exogenous resevoir into the outer leaflet of the plasma membrane rather than a fusion of bilayer vectors containing the probes with the plasma membrane. This problem has been addressed by Gadella et al. (1999) who established a protocol for incorporation of NBD-phospholipid analogues into pl ...
... from an exogenous resevoir into the outer leaflet of the plasma membrane rather than a fusion of bilayer vectors containing the probes with the plasma membrane. This problem has been addressed by Gadella et al. (1999) who established a protocol for incorporation of NBD-phospholipid analogues into pl ...
Allogeneic T regulatory cell–mediated
... Treg cells in patients undergoing human leukocyte antigen– matched allogeneic bone marrow transplantation.24 Thus, there is heightened interest in the basis by which allogeneic Treg cells mediate transplantation tolerance. ...
... Treg cells in patients undergoing human leukocyte antigen– matched allogeneic bone marrow transplantation.24 Thus, there is heightened interest in the basis by which allogeneic Treg cells mediate transplantation tolerance. ...
Leading to a Mild Immunodeficiency Activator trans
... The expression of MHC class II molecules is essential for all Ag-dependent immune functions and is regulated at the transcriptional level. Four trans-acting proteins control the coordinate expression of MHC class II molecules: class II trans-activator (CIITA), regulatory factor binding to the X box ...
... The expression of MHC class II molecules is essential for all Ag-dependent immune functions and is regulated at the transcriptional level. Four trans-acting proteins control the coordinate expression of MHC class II molecules: class II trans-activator (CIITA), regulatory factor binding to the X box ...
135272909 - (BORA)
... Acute myeloid leukemia (AML) is an aggressive heterogeneous cancer of the peripheral blood and the bone marrow, represented by a clonal expansion and accumulation of immature myeloid progenitor cells (blasts). (1) This loss of normal hematopoietic proliferation and differentiation leads to defects i ...
... Acute myeloid leukemia (AML) is an aggressive heterogeneous cancer of the peripheral blood and the bone marrow, represented by a clonal expansion and accumulation of immature myeloid progenitor cells (blasts). (1) This loss of normal hematopoietic proliferation and differentiation leads to defects i ...
The Drosophila wing differentiation factor Vestigial
... involved in DNA replication as well as cell cycle progression.19,20 In Drosophila, it has been shown that dE2F1 (called here dE2F) regulates cell cycle progression from G1 to S and its overexpression can drive cells from G2 to M by activating the expression of string (stg), the homolog of yeast cdc2 ...
... involved in DNA replication as well as cell cycle progression.19,20 In Drosophila, it has been shown that dE2F1 (called here dE2F) regulates cell cycle progression from G1 to S and its overexpression can drive cells from G2 to M by activating the expression of string (stg), the homolog of yeast cdc2 ...
Fgf8 induces pillar cell fate and regulates cellular
... Embryos that were Fgf8⌬2,3n/flox; Foxg1cre/+ (see Materials and methods for specific genetic cross) die at birth as a result of defects in the development of the forebrain (Storm et al., 2003); however, overall development of the inner ear and cochlea appeared normal. To examine the effects of inact ...
... Embryos that were Fgf8⌬2,3n/flox; Foxg1cre/+ (see Materials and methods for specific genetic cross) die at birth as a result of defects in the development of the forebrain (Storm et al., 2003); however, overall development of the inner ear and cochlea appeared normal. To examine the effects of inact ...
Sperm entry is sufficient to trigger division of the
... In conclusion, we propose that cdka;1 does not affect sperm cell fate but delays the pace of the cell cycle, leading to the production of one or two sperm cells with altered chromatin composition. Determination of the timing of transcriptional onset after fertilization HTR10 is a very divergent H3 v ...
... In conclusion, we propose that cdka;1 does not affect sperm cell fate but delays the pace of the cell cycle, leading to the production of one or two sperm cells with altered chromatin composition. Determination of the timing of transcriptional onset after fertilization HTR10 is a very divergent H3 v ...
Insulin and insulin-like growth factor I (IGF I) in early
... structurally and biochemically similar to pancreatic insulin but different from IGF I and IGF II (Yamada and Serrero, 1988). The same authors showed that IRF acts as an autocrine growth factor for these cells. Since teratoma and teratocarcinoma cell lines produce several hormones and growth factors ...
... structurally and biochemically similar to pancreatic insulin but different from IGF I and IGF II (Yamada and Serrero, 1988). The same authors showed that IRF acts as an autocrine growth factor for these cells. Since teratoma and teratocarcinoma cell lines produce several hormones and growth factors ...
Trafficking of the human transferrin receptor in plant cells: effects of
... *For correspondence (fax þ34 963544917; e-mail fernando.aniento@uv.es). ...
... *For correspondence (fax þ34 963544917; e-mail fernando.aniento@uv.es). ...
Microtubule
... A defining characteristic of living organisms is their ability to proliferate and the very fundamental basis is the division of a single cell into two daughter cells with exact complements of the parental genetic material. (Figure 3) At the beginning of the early prophase of the cell cycle, centroso ...
... A defining characteristic of living organisms is their ability to proliferate and the very fundamental basis is the division of a single cell into two daughter cells with exact complements of the parental genetic material. (Figure 3) At the beginning of the early prophase of the cell cycle, centroso ...
Conclusion - Computing Services
... internalization for presentation was used as a positive control. Results: Phenytoin induced lymphoproliferation in PBMC from all 6 patients but no control patients (median SI = 19.5, range 7.4 -130). Responding cells from PBMC and TCC were CD4+ and produced large amounts of IFN- and IL-5. TCC (N=17 ...
... internalization for presentation was used as a positive control. Results: Phenytoin induced lymphoproliferation in PBMC from all 6 patients but no control patients (median SI = 19.5, range 7.4 -130). Responding cells from PBMC and TCC were CD4+ and produced large amounts of IFN- and IL-5. TCC (N=17 ...
Interleukin-2
... doses were not well tolerated. Starting doses of 9-15 MIU were preferred in more recent CIV IL-2 studies. Production aspects of aldesleukin Commercial available aldesleukin (Proleukin) is the product of recombinant DNA technology. When compared to other methods such as stimulation of certain cells ...
... doses were not well tolerated. Starting doses of 9-15 MIU were preferred in more recent CIV IL-2 studies. Production aspects of aldesleukin Commercial available aldesleukin (Proleukin) is the product of recombinant DNA technology. When compared to other methods such as stimulation of certain cells ...
ATM and - Cancer Research
... and followed by washing in PBS. Incubations with antimouse tetramethylrhodamine isothiocyanate and FITC or with antirabbit FITC secondary antibodies (Sigma-Aldrich) were performed at 37°C at 1:100 dilutions in 2% bovine serum fraction V albumin for 20 min. Nuclei were counterstained with 4⬘,6diamidi ...
... and followed by washing in PBS. Incubations with antimouse tetramethylrhodamine isothiocyanate and FITC or with antirabbit FITC secondary antibodies (Sigma-Aldrich) were performed at 37°C at 1:100 dilutions in 2% bovine serum fraction V albumin for 20 min. Nuclei were counterstained with 4⬘,6diamidi ...
RUNX/AML and C/EBP factors regulate CD11a
... antiserum against RUNX1; generously provided by Dr N. A. Speck), or an anti-RUNX3 polyclonal antiserum47 was incubated with the nuclear extracts at 4°C for 30 minutes before the addition of the probe. Nuclear extracts were prepared according to Schreiber et al48 but including aprotinin, antipain, le ...
... antiserum against RUNX1; generously provided by Dr N. A. Speck), or an anti-RUNX3 polyclonal antiserum47 was incubated with the nuclear extracts at 4°C for 30 minutes before the addition of the probe. Nuclear extracts were prepared according to Schreiber et al48 but including aprotinin, antipain, le ...
SNX9 – a prelude to vesicle release - Journal of Cell Science
... that opens up the possibility that the second site has specificity for PtdIns(4,5)P2. Further structural studies are required to fully decipher the phosphoinositide-binding properties of SNX9. In pure protein-liposome assays, the yoke-PX-BAR structural unit has a high capacity to modulate spherical ...
... that opens up the possibility that the second site has specificity for PtdIns(4,5)P2. Further structural studies are required to fully decipher the phosphoinositide-binding properties of SNX9. In pure protein-liposome assays, the yoke-PX-BAR structural unit has a high capacity to modulate spherical ...
Pedomicrobium - International Journal of Systematic and
... logarithmic growth phase, 1 volume of culture was transferred to 100 volumes of the test medium and incubated. Again from cultures in the logarithmic growth phase, cell suspensions were inoculated 1:100 (vol/vol) into fresh test medium. At the end of the log phase of growth, turbidity, flocculation, ...
... logarithmic growth phase, 1 volume of culture was transferred to 100 volumes of the test medium and incubated. Again from cultures in the logarithmic growth phase, cell suspensions were inoculated 1:100 (vol/vol) into fresh test medium. At the end of the log phase of growth, turbidity, flocculation, ...
ISG15, a ubiquitin-like interferon
... 2005). A functional association between this pathway and the regulation of HCV production has been established: knockdown of USP18 increases the anti-HCV potency of IFN-a (Randall et al., 2006), and ISG15 protein expression is highly upregulated in the hepatocytes of treatment nonresponders, but is ...
... 2005). A functional association between this pathway and the regulation of HCV production has been established: knockdown of USP18 increases the anti-HCV potency of IFN-a (Randall et al., 2006), and ISG15 protein expression is highly upregulated in the hepatocytes of treatment nonresponders, but is ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.