Passive transfer of anti-herpes simplex virus type 2
... Purpose. To investigate whether passive transfer of antibodies to viral glycoproteins would protect against herpes simplex virus type 2-induced stromal keratitis. Methods. Balb/c mice were infected on the scarified cornea with herpes simplex virus types 1 or 2 (HSV-1 and HSV-2, respectively), and mo ...
... Purpose. To investigate whether passive transfer of antibodies to viral glycoproteins would protect against herpes simplex virus type 2-induced stromal keratitis. Methods. Balb/c mice were infected on the scarified cornea with herpes simplex virus types 1 or 2 (HSV-1 and HSV-2, respectively), and mo ...
STEROIDOGENESIS IN THECA CELLS OF CHICKEN FOLLICLES
... used collagenase for the isolation of theca cells. As the damage to cells by enzymatical dispersion is generally known to be greater by trypsin than by collagenase, the difference in enzyme used may explain the discrepancy for the theca interna cell response to gonadotropins between our and their r ...
... used collagenase for the isolation of theca cells. As the damage to cells by enzymatical dispersion is generally known to be greater by trypsin than by collagenase, the difference in enzyme used may explain the discrepancy for the theca interna cell response to gonadotropins between our and their r ...
Protein kinase Ca activation by RET: evidence for a negative
... substrates were from Calbiochem-Novabiochem (La Jolla, CA, USA). Bisindolylmaleimide was from Alexis, Inc. (San Diego, CA, USA). Protein electrophoresis reagents were from Bio-Rad (Richmond, VA, USA). Western blotting and ECL reagents were from Amersham (Arlinghton Heights, IL, USA). All other chemi ...
... substrates were from Calbiochem-Novabiochem (La Jolla, CA, USA). Bisindolylmaleimide was from Alexis, Inc. (San Diego, CA, USA). Protein electrophoresis reagents were from Bio-Rad (Richmond, VA, USA). Western blotting and ECL reagents were from Amersham (Arlinghton Heights, IL, USA). All other chemi ...
Microsoft Word
... most likely due to replication errors [10]. The question arises which factors determine that primary oxidative lesions in mtDNA are converted either to mitochondrial point mutations or to rearrangements of the mitochondrial genome. As reactive oxygen species (ROS) produced in mitochondria can lead t ...
... most likely due to replication errors [10]. The question arises which factors determine that primary oxidative lesions in mtDNA are converted either to mitochondrial point mutations or to rearrangements of the mitochondrial genome. As reactive oxygen species (ROS) produced in mitochondria can lead t ...
Structural and functional classes of multipolar cells in the ventral
... ultrastructural profiles observed with the electron microscope resulted in the division of VCN multipolar cells in cats (Cant, 1981). Type I multipolar neurons receive very few synaptic contacts on their cell body, whereas Type II somata are covered with synaptic endings (Fig. 1A). Second, the parent ...
... ultrastructural profiles observed with the electron microscope resulted in the division of VCN multipolar cells in cats (Cant, 1981). Type I multipolar neurons receive very few synaptic contacts on their cell body, whereas Type II somata are covered with synaptic endings (Fig. 1A). Second, the parent ...
Extrinsic Factors Influence the Expression of Voltage
... the absenceof other cell types, suggestingthat some factor(s) necessaryfor the expressionof thesecurrents is missingin these cultures (McFarlane and Cooper, 1992). One possibility is that non-neuronal cells from the ganglion provide a factor(s) that affectsA-current expression.To test this, we measu ...
... the absenceof other cell types, suggestingthat some factor(s) necessaryfor the expressionof thesecurrents is missingin these cultures (McFarlane and Cooper, 1992). One possibility is that non-neuronal cells from the ganglion provide a factor(s) that affectsA-current expression.To test this, we measu ...
PDF - Potter Lab
... differentiation. It was suggested that the large cell phenotype may be caused by endoreplication (Ito and Rubin, 1999). Moreover, organs that contain a majority of Tsc2 mutant cells were increased in size. However, the functions of Drosophila Tsc1 remained uncharacterized. Recent studies have indica ...
... differentiation. It was suggested that the large cell phenotype may be caused by endoreplication (Ito and Rubin, 1999). Moreover, organs that contain a majority of Tsc2 mutant cells were increased in size. However, the functions of Drosophila Tsc1 remained uncharacterized. Recent studies have indica ...
Mycobacterium tuberculosis adhesins: potential
... & Hensel, 2007). Fimbriae (hairs) or pili (threads) are the most common form of bacterial adhesins. They are elongated, multi-subunit protein structures that are able to interact with glycoprotein and glycolipid receptors found on host cells (Esko & Sharon, 2009). To be functional, adhesins must be ...
... & Hensel, 2007). Fimbriae (hairs) or pili (threads) are the most common form of bacterial adhesins. They are elongated, multi-subunit protein structures that are able to interact with glycoprotein and glycolipid receptors found on host cells (Esko & Sharon, 2009). To be functional, adhesins must be ...
Golgi clusters and vesicles mediate mitotic inheritance
... example, those fragments indicated by an arrow and an arrowhead in Fig. 3 A) were summed and found to account for only 40–50% of the Golgi membrane that was eventually redistributed based on quantitative assessment of total cellular fluorescence by image analysis. These findings were corroborated us ...
... example, those fragments indicated by an arrow and an arrowhead in Fig. 3 A) were summed and found to account for only 40–50% of the Golgi membrane that was eventually redistributed based on quantitative assessment of total cellular fluorescence by image analysis. These findings were corroborated us ...
Copper tolerance of the thermoacidophilic archaeon
... accompanied by cation transport would be removed from the cell through the Pit system. We have recently found that Acidithiobacillus ferrooxidans, a micro-organism that can tolerate very high concentrations of heavy metals in its normal environment, normally accumulates high amounts of polyP granule ...
... accompanied by cation transport would be removed from the cell through the Pit system. We have recently found that Acidithiobacillus ferrooxidans, a micro-organism that can tolerate very high concentrations of heavy metals in its normal environment, normally accumulates high amounts of polyP granule ...
Barbiturates Directly Inhibit the Calmodulin/Calcineurin Complex: a
... and cell biological evidence support the notion that the serine/threonine phosphatase calcineurin is a rate-limiting intracellular signaling enzyme involved in TCR-mediated signal transduction (Batiuk et al., 1997). Calcineurin activity is regulated by the intracellular concentration of calcium and ...
... and cell biological evidence support the notion that the serine/threonine phosphatase calcineurin is a rate-limiting intracellular signaling enzyme involved in TCR-mediated signal transduction (Batiuk et al., 1997). Calcineurin activity is regulated by the intracellular concentration of calcium and ...
Phosphatidylcholine traffic to the vacuole
... Lyles, 2000). These lipid analogs have proven useful for studying lipid transport and trafficking events. For example, in many cell lines, phosphatidylethanolamine (PtdEtn) and PtdSer analogs are internalized rapidly by flip whereas phosphatidylcholine (PtdCho) is internalized by flip slowly, if at ...
... Lyles, 2000). These lipid analogs have proven useful for studying lipid transport and trafficking events. For example, in many cell lines, phosphatidylethanolamine (PtdEtn) and PtdSer analogs are internalized rapidly by flip whereas phosphatidylcholine (PtdCho) is internalized by flip slowly, if at ...
Role of Template Activating Factor-I as a
... separated by 10% SDS-PAGE and visualized with Coomassie Brilliant Blue staining. Lane M contains molecular size markers. (B) Formation of histone H1.1NCPs complexes was examined by nucleoprotein gel analyses. The 196 bp 5S rRNA gene fragments or NCPs (0.4 pmol of DNA) assembled on the same DNA with ...
... separated by 10% SDS-PAGE and visualized with Coomassie Brilliant Blue staining. Lane M contains molecular size markers. (B) Formation of histone H1.1NCPs complexes was examined by nucleoprotein gel analyses. The 196 bp 5S rRNA gene fragments or NCPs (0.4 pmol of DNA) assembled on the same DNA with ...
Inflammation and proliferation – a causal event of host response to
... variety of extracellular stimuli through a cascade of protein phosphorylations leading to the activation of transcription factors. The MAPK signal transduction pathway plays a crucial role in many aspects of immune-mediated inflammatory responses. The involvement of MAPK pathways in NFkB activation ...
... variety of extracellular stimuli through a cascade of protein phosphorylations leading to the activation of transcription factors. The MAPK signal transduction pathway plays a crucial role in many aspects of immune-mediated inflammatory responses. The involvement of MAPK pathways in NFkB activation ...
Microtubules Regulate Dynamic Organization of Vacuoles in
... actin microfilaments, rather than microtubules, and that their maintenance and distribution are actin dependent (Ovecka et al. 2005, Higaki et al. 2006). To investigate vacuolar morphology and its regulatory mechanisms, we have, in this study, established a new visualization system using the moss, Ph ...
... actin microfilaments, rather than microtubules, and that their maintenance and distribution are actin dependent (Ovecka et al. 2005, Higaki et al. 2006). To investigate vacuolar morphology and its regulatory mechanisms, we have, in this study, established a new visualization system using the moss, Ph ...
Functional Analysis of the Cellulose Synthase
... was observed for any of the mutants in CSLD2 or CSLD3. The roots of 4-d-old wild-type Col-0 seedlings produced root hairs of varying lengths, as shown in Figure 1, A and D. In contrast, the root hairs of csld2-1 seedlings were shorter than those of wild-type controls, and most had obvious visible de ...
... was observed for any of the mutants in CSLD2 or CSLD3. The roots of 4-d-old wild-type Col-0 seedlings produced root hairs of varying lengths, as shown in Figure 1, A and D. In contrast, the root hairs of csld2-1 seedlings were shorter than those of wild-type controls, and most had obvious visible de ...
Chapter 3 Cells and Tissues
... • Protein synthesis—occurs in cytoplasm, thus genetic information must pass from the nucleus to the cytoplasm • Process of transferring genetic information from nucleus to cytoplasm where proteins are produced requires completion of transcription and translation ...
... • Protein synthesis—occurs in cytoplasm, thus genetic information must pass from the nucleus to the cytoplasm • Process of transferring genetic information from nucleus to cytoplasm where proteins are produced requires completion of transcription and translation ...
Functional and phylogenetic analyses of chromosome 21 promoters
... Starting from a single cell, multi-cellular organisms develop into complex systems composed of various different cell types all equipped with the same set of genes. Yet each cell employs only a part of the genes at any given moment. During development and life, the proportion and composition of expr ...
... Starting from a single cell, multi-cellular organisms develop into complex systems composed of various different cell types all equipped with the same set of genes. Yet each cell employs only a part of the genes at any given moment. During development and life, the proportion and composition of expr ...
Figure 2 - Trace: Tennessee Research and Creative Exchange
... LBR, ligand binding region. The asterisk ‘*’ indicates that in a few cases only an LBR at the C-terminal extension of the TM regions is found (Lacal et al., 2010). ...
... LBR, ligand binding region. The asterisk ‘*’ indicates that in a few cases only an LBR at the C-terminal extension of the TM regions is found (Lacal et al., 2010). ...
Visualization of an endogenous retinoic acid gradient across
... centre). Comparative spatial profiles indicated that the signals representing [RA]i were high in the raldh2-expressing region and low in the cyp26s-expressing region (Fig. 1d, right). Two intermediate zones flanked by the raldh2-expressing and cyp26s-expressing regions were of particular interest. I ...
... centre). Comparative spatial profiles indicated that the signals representing [RA]i were high in the raldh2-expressing region and low in the cyp26s-expressing region (Fig. 1d, right). Two intermediate zones flanked by the raldh2-expressing and cyp26s-expressing regions were of particular interest. I ...
Automatic sampling for unbiased and efficient stereological
... The correlation between the biased weight of a field of view and the correct count in the field may be positive or negative. Regardless of that, the estimate is always unbiased. The precision (CE) is, however, much dependent on the relationship between weight and count: the more positive the better ...
... The correlation between the biased weight of a field of view and the correct count in the field may be positive or negative. Regardless of that, the estimate is always unbiased. The precision (CE) is, however, much dependent on the relationship between weight and count: the more positive the better ...
CURRICULUM VITAE
... 28. Braunwald J, Nonnenmacher CA, Pereira CA, Kirn A. - Increased susceptibility to mouse hepatitis virus type 3 (MHV3) infection induced by a hypercholesterolemic diet with increased adsorption of MHV3 to primary hepatocyte cultures. Res.Virol. 1991, 142:5-15. 29. Lucchiari MA, Martin JP, Modollel ...
... 28. Braunwald J, Nonnenmacher CA, Pereira CA, Kirn A. - Increased susceptibility to mouse hepatitis virus type 3 (MHV3) infection induced by a hypercholesterolemic diet with increased adsorption of MHV3 to primary hepatocyte cultures. Res.Virol. 1991, 142:5-15. 29. Lucchiari MA, Martin JP, Modollel ...
Chapter 3 PowerPoint
... Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings ...
... Copyright © 2007 Pearson Education, Inc., publishing as Benjamin Cummings ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.