Subtype-specific regulation of equilibrative nucleoside transporters
... identified mouse ENT1 splice variants that differ in having either one or two consensus sites for phosphorylation by CK2. Preliminary data suggest that the variant with only one CK2 site has reduced transport functionality relative to the variant with both CK2 consensus sites (M. Stolk and J. R. Ham ...
... identified mouse ENT1 splice variants that differ in having either one or two consensus sites for phosphorylation by CK2. Preliminary data suggest that the variant with only one CK2 site has reduced transport functionality relative to the variant with both CK2 consensus sites (M. Stolk and J. R. Ham ...
Deciphering Transcriptional Regulatory Elements that Encode
... Scanning promoters for TF hits. Promoter sequences were scanned by searching for matches of each regular expression in both strands of a predefined interval around the TSS. The intervals used were (positions are relative to the TSS, negative positions are upstream to the TSS): E2F: from -300 to +100 ...
... Scanning promoters for TF hits. Promoter sequences were scanned by searching for matches of each regular expression in both strands of a predefined interval around the TSS. The intervals used were (positions are relative to the TSS, negative positions are upstream to the TSS): E2F: from -300 to +100 ...
Guidleine on potency testing of cell based immunotherapy medicinal
... the biological activity of the active ingredient either at the level of drug substance and/or drug product. In principle the results of a potency assay should provide assurance that the amount of the active ingredient is sufficient to induce a meaningful response and that the amount is consistent fr ...
... the biological activity of the active ingredient either at the level of drug substance and/or drug product. In principle the results of a potency assay should provide assurance that the amount of the active ingredient is sufficient to induce a meaningful response and that the amount is consistent fr ...
Cue-Signal-Response Analysis of TNF-Induced
... Cue–signal–response relationships in cell signaling are ripe for applying multivariate analysis techniques, such as PLS, to tease out the key biochemical changes associated with critical cell decisions. However, assaying cell signaling involves the measurement of a wide range of parameters including ...
... Cue–signal–response relationships in cell signaling are ripe for applying multivariate analysis techniques, such as PLS, to tease out the key biochemical changes associated with critical cell decisions. However, assaying cell signaling involves the measurement of a wide range of parameters including ...
Intrusive growth of flax phloem fibers is of intercalary type
... (PBS) for two 5 min washes. Free aldehydes were reduced by treatment with 0.1 M hydroxyltetra-ammoniumchloride (HAH) in PBS for 10 min. Samples were incubated in 0.1% acetylated BSA (blocking agent, Aurion, Wageningen, NL) in PBS for 15 min for blocking negatively charged sites and ions. Then, they ...
... (PBS) for two 5 min washes. Free aldehydes were reduced by treatment with 0.1 M hydroxyltetra-ammoniumchloride (HAH) in PBS for 10 min. Samples were incubated in 0.1% acetylated BSA (blocking agent, Aurion, Wageningen, NL) in PBS for 15 min for blocking negatively charged sites and ions. Then, they ...
Structure and function of nucleus-vacuole junctions: outer
... The endoplasmic reticulum (ER) is an interconnected membrane network composed of structurally distinct compartments, including the peripheral or cortical ER and the perinuclear ER, which surrounds the nucleus (reviewed in Voeltz et al., 2002). Although these compartments are topologically continuous ...
... The endoplasmic reticulum (ER) is an interconnected membrane network composed of structurally distinct compartments, including the peripheral or cortical ER and the perinuclear ER, which surrounds the nucleus (reviewed in Voeltz et al., 2002). Although these compartments are topologically continuous ...
Latrunculin A Induced Perturbation of the Actin Cytoskeleton
... Pap1p translocates from the cytoplasm to the nucleus upon LatA treatment Previous studies concerning the role of pap1, imp1, and caf5 in the oxidative stress response (Umeda et al., 2005) suggested a straightforward model to be tested. In this model the Pap1p transcription factor is imported into th ...
... Pap1p translocates from the cytoplasm to the nucleus upon LatA treatment Previous studies concerning the role of pap1, imp1, and caf5 in the oxidative stress response (Umeda et al., 2005) suggested a straightforward model to be tested. In this model the Pap1p transcription factor is imported into th ...
The Par3/Par6/aPKC Complex and Epithelial Cell Polarity
... the organism, and allow vectorial transport from one side to the other. To achieve this function, epithelial cells must be highly polarized. Therefore at the outermost layer of a cell-plasma membrane, we could observe different shape and polarized distribution of molecules. The plasma membrane of ep ...
... the organism, and allow vectorial transport from one side to the other. To achieve this function, epithelial cells must be highly polarized. Therefore at the outermost layer of a cell-plasma membrane, we could observe different shape and polarized distribution of molecules. The plasma membrane of ep ...
Cell Cycle - CiteSeerX
... Scanning promoters for TF hits. Promoter sequences were scanned by searching for matches of each regular expression in both strands of a predefined interval around the TSS. The intervals used were (positions are relative to the TSS, negative positions are upstream to the TSS): E2F: from -300 to +100 ...
... Scanning promoters for TF hits. Promoter sequences were scanned by searching for matches of each regular expression in both strands of a predefined interval around the TSS. The intervals used were (positions are relative to the TSS, negative positions are upstream to the TSS): E2F: from -300 to +100 ...
T Cells + Memory CD8 Influenza A Virus
... cells have a higher avidity TCR-peptide-MHC interaction when compared with their CD8⫹DbNP366 counterparts (13). This interaction is characterized by a slower tetramer dissociation rate and less dependence on the CD8 coreceptor for IFN-␥ production. Furthermore, the CD8⫹DbPA224 set produces signific ...
... cells have a higher avidity TCR-peptide-MHC interaction when compared with their CD8⫹DbNP366 counterparts (13). This interaction is characterized by a slower tetramer dissociation rate and less dependence on the CD8 coreceptor for IFN-␥ production. Furthermore, the CD8⫹DbPA224 set produces signific ...
The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
... subunit by coimmunoprecipitation with the remaining subunits (Guo et al., 1999a). Electron microscopy and analyses of cargo protein trafficking in yeast exo84 mutants indicated that Exo84 is involved in the post-Golgi stage of secretion (Zhang et al., 2005b). The same study demonstrated that Exo84 p ...
Ch. 27 & 28 Notes
... 27.1 ~ Structural, functional, and genetic adaptations contribute to prokaryotic success ...
... 27.1 ~ Structural, functional, and genetic adaptations contribute to prokaryotic success ...
Impact of clostridial glucosylating toxins on the
... proteins by glucosylation of a pivotal threonine residue. Purified toxins induce reorganization of the cytoskeleton and cell death in colonic cells. Whether all toxin effects on target cells depend on catalytic glucosyltransferase activity is unclear at present. Thus, we conducted a proteome approac ...
... proteins by glucosylation of a pivotal threonine residue. Purified toxins induce reorganization of the cytoskeleton and cell death in colonic cells. Whether all toxin effects on target cells depend on catalytic glucosyltransferase activity is unclear at present. Thus, we conducted a proteome approac ...
Characterization of the unconventional myosin VIII in plant cells and
... class of myosin contains tail domains which are characteristic for each myosin and are believed to be responsible for the speci®c subcellular localization and function of these motors. In unicellular systems and mammalian cells the involvement of the conventional two-headed, ®lamentforming myosins ( ...
... class of myosin contains tail domains which are characteristic for each myosin and are believed to be responsible for the speci®c subcellular localization and function of these motors. In unicellular systems and mammalian cells the involvement of the conventional two-headed, ®lamentforming myosins ( ...
FREE Sample Here
... Section: 6.6 47) Researchers tried to explain how vesicular transport occurs in cells by attempting to assemble the transport components. They set up microtubular tracks along which vesicles could be transported, and they added vesicles and ATP (because they knew the transport process requires energ ...
... Section: 6.6 47) Researchers tried to explain how vesicular transport occurs in cells by attempting to assemble the transport components. They set up microtubular tracks along which vesicles could be transported, and they added vesicles and ATP (because they knew the transport process requires energ ...
Bacillus globigii cell size is influenced by
... PLWCA on the size of B. globigii cells. Filled columns represent the results after 16 h incubation of B. globigii with the peptides; open columns represent the 6 h growth after the 16 h incubations were re-inoculated in fresh medium. *** p \ 0.001. b Typical examples of control cells (left) and cell ...
... PLWCA on the size of B. globigii cells. Filled columns represent the results after 16 h incubation of B. globigii with the peptides; open columns represent the 6 h growth after the 16 h incubations were re-inoculated in fresh medium. *** p \ 0.001. b Typical examples of control cells (left) and cell ...
Sample pages 1 PDF
... and applied aspects. SE occurs in nature. At the edges of the leaves of several species of Kalanchoë appear small bipolar structures, some of them produce these structures constitutively, others by the action of environmental stress, and a third group is a combination of both (Garces and Sinha 2009) ...
... and applied aspects. SE occurs in nature. At the edges of the leaves of several species of Kalanchoë appear small bipolar structures, some of them produce these structures constitutively, others by the action of environmental stress, and a third group is a combination of both (Garces and Sinha 2009) ...
A novel giant peroxisomal superoxide dismutase motif
... were amplified from mouse brain cDNA using the following specific primers: 5′-ACCACAGTCCATGCCATCAC-3′ and 5′-TCCACCACCCTGTTGCTGTA-3′ for Gapdh, 5′-TGTTCGCTGTCTCCAGGTTATTC-3′ and 5′-GCTTGCCTCACTGTATGACTT-3′ for xCT, 5′-AGAGGAAGGAGGCGCATCA-3′ and 5′-CTGGGCCACTTTCATGTTCTCGT-3′ for the γ-GCS catalytic sub ...
... were amplified from mouse brain cDNA using the following specific primers: 5′-ACCACAGTCCATGCCATCAC-3′ and 5′-TCCACCACCCTGTTGCTGTA-3′ for Gapdh, 5′-TGTTCGCTGTCTCCAGGTTATTC-3′ and 5′-GCTTGCCTCACTGTATGACTT-3′ for xCT, 5′-AGAGGAAGGAGGCGCATCA-3′ and 5′-CTGGGCCACTTTCATGTTCTCGT-3′ for the γ-GCS catalytic sub ...
The fission yeast cdc19+ gene encodes a member of the MCM
... al., 1992). Intriguingly, these gene products all undergo a cellcycle-specific nuclear localization near the G1/S transition (Hennessy et al., 1990; Yan et al., 1993). Such controlled nuclear entry could provide another means of regulating the G1 to S phase transition and maintaining the order of ev ...
... al., 1992). Intriguingly, these gene products all undergo a cellcycle-specific nuclear localization near the G1/S transition (Hennessy et al., 1990; Yan et al., 1993). Such controlled nuclear entry could provide another means of regulating the G1 to S phase transition and maintaining the order of ev ...
A Novel Role of Annexin A2 in Human Type I Collagen Gene
... The fibrillar collagen scaffold of the extracellular matrix provides a structural framework for cells in tissues and regulates intercellular communication; its disregulation has been associated with tumour development and progression. Previous work has shown that expression of type I collagen, the mo ...
... The fibrillar collagen scaffold of the extracellular matrix provides a structural framework for cells in tissues and regulates intercellular communication; its disregulation has been associated with tumour development and progression. Previous work has shown that expression of type I collagen, the mo ...
Concepts of Biology
... The microscopes we use today are far more complex than those used in the 1600s by Antony van Leeuwenhoek, a Dutch shopkeeper who had great skill in crafting lenses. Despite the limitations of his now-ancient lenses, van Leeuwenhoek observed the movements of protists (a type of single-celled organism ...
... The microscopes we use today are far more complex than those used in the 1600s by Antony van Leeuwenhoek, a Dutch shopkeeper who had great skill in crafting lenses. Despite the limitations of his now-ancient lenses, van Leeuwenhoek observed the movements of protists (a type of single-celled organism ...
Genetic transformation of HeLa cells by Agrobacterium - igem
... whether Agrobacterium transfers T-DNA to human cells, we constructed the binary vector pNeo. This plasmid carries a T-DNA that contains a neomycin resistance gene driven by the Simian-virus-40 early promoter, which is specific for mammalian cells. pNeo was introduced then into the Agrobacterium help ...
... whether Agrobacterium transfers T-DNA to human cells, we constructed the binary vector pNeo. This plasmid carries a T-DNA that contains a neomycin resistance gene driven by the Simian-virus-40 early promoter, which is specific for mammalian cells. pNeo was introduced then into the Agrobacterium help ...
22%-efficiency hit solar cell
... By inserting the high-quality intrinsic a-Si layer, the defects on the c-Si surface can be effectively passivated, and a high Voc can be obtained. This solar cell is based on Sanyo’s technologies for forming high-quality a-Si films and a-Si solar cells with low-plasma and lowthermal damage processes ...
... By inserting the high-quality intrinsic a-Si layer, the defects on the c-Si surface can be effectively passivated, and a high Voc can be obtained. This solar cell is based on Sanyo’s technologies for forming high-quality a-Si films and a-Si solar cells with low-plasma and lowthermal damage processes ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.