Involvement of Native TRPC3 Proteins in ATP
... constitutive cation influx with 2 mmol/L Ca2⫹ in the bath likely reflects operation of a highly efficient Ca2⫹ buffering system. In line with this, when cells were exposed to higher Ca2⫹ gradients (10 mmol/L in the bath), a significant yet transient Ca2⫹ influx was observed (not shown). Ba2⫹ is not ...
... constitutive cation influx with 2 mmol/L Ca2⫹ in the bath likely reflects operation of a highly efficient Ca2⫹ buffering system. In line with this, when cells were exposed to higher Ca2⫹ gradients (10 mmol/L in the bath), a significant yet transient Ca2⫹ influx was observed (not shown). Ba2⫹ is not ...
Cytoskeletal Motors in Arabidopsis. Sixty
... classified as At1 (Dagenbach and Endow, 2004). The tobacco (Nicotiana tabacum) homolog NACK1 physically interacts with a MAP kinase kinase kinase called NPK1, and they form a complex during cell division (Nishihama et al., 2002). Both NACK1 and NPK1 localize to the midline in the phragmoplast of tob ...
... classified as At1 (Dagenbach and Endow, 2004). The tobacco (Nicotiana tabacum) homolog NACK1 physically interacts with a MAP kinase kinase kinase called NPK1, and they form a complex during cell division (Nishihama et al., 2002). Both NACK1 and NPK1 localize to the midline in the phragmoplast of tob ...
Hypothetical photosensory structure in ciliated protozoan, Blepharisma
... the cell surface, i.e. the smooth endocyst layers beneath the smooth cell surface (Figs 6A, B) and undulate layers for furrowed cell surface (Figs 6D, E). The alternative formation of the narrow and broad layers of endocyst may be responsible for the periodic shrinkage of the cell body. That is, bro ...
... the cell surface, i.e. the smooth endocyst layers beneath the smooth cell surface (Figs 6A, B) and undulate layers for furrowed cell surface (Figs 6D, E). The alternative formation of the narrow and broad layers of endocyst may be responsible for the periodic shrinkage of the cell body. That is, bro ...
Sulfated polysaccharides from marine sponges
... Marine sponges (Porifera) are ancient and simple eumetazoans. They constitute key organisms in the evolution from unicellular to multicellular animals. We now demonstrated that pure sulfated polysaccharides from marine sponges are responsible for the species-specific cell–cell interaction in these i ...
... Marine sponges (Porifera) are ancient and simple eumetazoans. They constitute key organisms in the evolution from unicellular to multicellular animals. We now demonstrated that pure sulfated polysaccharides from marine sponges are responsible for the species-specific cell–cell interaction in these i ...
RalA ACTIVATION ASSAY BIOCHEM KIT
... The Ras family of small GTPases consists of at least 35 related human proteins that include the oncoproteins HRAS, KRAS and NRAS as the founding members (Colicelli 2004). The Ral proteins, RalA and RalB, share 58% identity to these oncoproteins and 85% identity with each other (Chardin and Tavitian ...
... The Ras family of small GTPases consists of at least 35 related human proteins that include the oncoproteins HRAS, KRAS and NRAS as the founding members (Colicelli 2004). The Ral proteins, RalA and RalB, share 58% identity to these oncoproteins and 85% identity with each other (Chardin and Tavitian ...
Characterization of transport systems for cysteine, lysine, alanine
... has been a key determinant of their success, allowing greater control over thermoregulation, sexual communication, camouflage, and protection from the physical and chemical environment (Hynd, 2000). The production of fibers from animals also represents a small but economically important component of ...
... has been a key determinant of their success, allowing greater control over thermoregulation, sexual communication, camouflage, and protection from the physical and chemical environment (Hynd, 2000). The production of fibers from animals also represents a small but economically important component of ...
in Murine Mast Cells by Recombinant Stem Cell Factor
... nmol) [3H]-AA-labeled E. coli membranes (New England Nuclear, Boston, MA). After incubation (90 min at 370C) in a water bath, the reaction was stopped by extracting lipids by the method of Bligh and Dyer (62). Free fatty acids were isolated from phospholipids by TLC on silica gel G developed in hexa ...
... nmol) [3H]-AA-labeled E. coli membranes (New England Nuclear, Boston, MA). After incubation (90 min at 370C) in a water bath, the reaction was stopped by extracting lipids by the method of Bligh and Dyer (62). Free fatty acids were isolated from phospholipids by TLC on silica gel G developed in hexa ...
The Bordetella pertussis adenylate cyclase toxin binds to T cells via
... induce down-regulation of the putative receptor, although no variation in the levels of either the 7 integrin or the TCR–CD3 complex was observed (Fig. S1 A). Together, the data suggest that CyaA binds to T cells via LFA-1. The fact that CyaA binding was enhanced in activated T cells (Fig. 1 E) sug ...
... induce down-regulation of the putative receptor, although no variation in the levels of either the 7 integrin or the TCR–CD3 complex was observed (Fig. S1 A). Together, the data suggest that CyaA binds to T cells via LFA-1. The fact that CyaA binding was enhanced in activated T cells (Fig. 1 E) sug ...
Monitoring metabolic responses to chemotherapy initiator mass spectrometry (NIMS) imaging
... in lymphoid cells by LC-MS/MS [13]. Still, sample preparation for such methods requires tissue extraction, sacrificing anatomical resolution for analytical sensitivity [14,15], highlighting the need for improved metabolomics methodologies. Advancements in mass spectrometry imaging (MSI) and profilin ...
... in lymphoid cells by LC-MS/MS [13]. Still, sample preparation for such methods requires tissue extraction, sacrificing anatomical resolution for analytical sensitivity [14,15], highlighting the need for improved metabolomics methodologies. Advancements in mass spectrometry imaging (MSI) and profilin ...
Determination of the Structure and Composition of the ` Sulphur
... elementary analyses of the ' sulphur granules ' and Actinomyces P 2 s organisms grown in vitro were done by Galbraith Laboratories Inc. (Knoxville, Tennessee). About 10-12 mg. dry weight of ' sulphur granules ' or Actinomyces bovis organisms were combusted in a porcelain crucible to constant weight ...
... elementary analyses of the ' sulphur granules ' and Actinomyces P 2 s organisms grown in vitro were done by Galbraith Laboratories Inc. (Knoxville, Tennessee). About 10-12 mg. dry weight of ' sulphur granules ' or Actinomyces bovis organisms were combusted in a porcelain crucible to constant weight ...
Sucrose Cleavage Pathways in Aspen Wood
... et al., 2006). In another example, co-immuno-detection of Arabidopsis SUS2 and E37 which is a polypeptide of the inner plastid envelope showed SUS association with plastids of Arabidopsis thaliana embryo, endosperm and seed coat (Nunez et al., 2008). In addition, immuno-localization studies in which ...
... et al., 2006). In another example, co-immuno-detection of Arabidopsis SUS2 and E37 which is a polypeptide of the inner plastid envelope showed SUS association with plastids of Arabidopsis thaliana embryo, endosperm and seed coat (Nunez et al., 2008). In addition, immuno-localization studies in which ...
Polar auxin transport and patterning
... auxin levels (Jonsson et al. 2006) and to make PIN1 levels auxin dependent (Smith et al. 2006). While several additional rules were needed to make the different phyllotaxis models run, these two appeared to be powerful in creating the patterns observed in nature. Intriguingly, polarity reversals at ...
... auxin levels (Jonsson et al. 2006) and to make PIN1 levels auxin dependent (Smith et al. 2006). While several additional rules were needed to make the different phyllotaxis models run, these two appeared to be powerful in creating the patterns observed in nature. Intriguingly, polarity reversals at ...
Cell Communication and Signaling
... Abstract Background: Host cell invasion by the foodborne pathogen Campylobacter jejuni is considered as one of the primary reasons of gut tissue damage, however, mechanisms and key factors involved in this process are widely unclear. It was reported that small Rho GTPases, including Cdc42, are acti ...
... Abstract Background: Host cell invasion by the foodborne pathogen Campylobacter jejuni is considered as one of the primary reasons of gut tissue damage, however, mechanisms and key factors involved in this process are widely unclear. It was reported that small Rho GTPases, including Cdc42, are acti ...
Identification of a novel population of muscle stem cells in mice
... centages of desmin-expressing cells were still observed between the populations. Unlike EP cells, most MDSC (60%) do not express the myogenic marker desmin, and a small population (10%) of these cells is Sca-1/CD34; this latter characteristic also is found in primary hematopoietic stem cells (Nak ...
... centages of desmin-expressing cells were still observed between the populations. Unlike EP cells, most MDSC (60%) do not express the myogenic marker desmin, and a small population (10%) of these cells is Sca-1/CD34; this latter characteristic also is found in primary hematopoietic stem cells (Nak ...
Chloroplast Tubules Visualized in Transplastomic Plants Expressing
... cells, tubules filled with GFP connect two or more chloroplasts (Fig. 2b). It is expected that such connections between chloroplasts would contribute to keep the concentration of inorganic ions, metabolic intermediates, proteins and enzymes uniform among several chloroplasts in the same cell. Transp ...
... cells, tubules filled with GFP connect two or more chloroplasts (Fig. 2b). It is expected that such connections between chloroplasts would contribute to keep the concentration of inorganic ions, metabolic intermediates, proteins and enzymes uniform among several chloroplasts in the same cell. Transp ...
Gutsy moves in mice: cellular and molecular dynamics of endoderm
... peroxidase resulted in the first conceptual reconstruction of the cellular dynamics involved in endoderm formation in mouse embryos [17,18]. In this set of studies, cells of the epiblast and VE were individually labelled, embryos incubated for various time periods and then the position of their cell ...
... peroxidase resulted in the first conceptual reconstruction of the cellular dynamics involved in endoderm formation in mouse embryos [17,18]. In this set of studies, cells of the epiblast and VE were individually labelled, embryos incubated for various time periods and then the position of their cell ...
Control of the Cell Cycle in Early Embryos
... At the end of oogenesis, oocytes exit from the cell cycle and arrest at the G2/M border of meiosis I. In clams, fertilization provides the extracellular signal that breaks this cell cycle arrest and initiates a series of rapid cell division cycles. In this paper, we consider two aspects of this proc ...
... At the end of oogenesis, oocytes exit from the cell cycle and arrest at the G2/M border of meiosis I. In clams, fertilization provides the extracellular signal that breaks this cell cycle arrest and initiates a series of rapid cell division cycles. In this paper, we consider two aspects of this proc ...
LvNotch specifies secondary mesenchyme - Development
... The level of endogenous LvNotch protein in 30 embryos was found to be slightly below the detection limit of α-LvNotch pAbs for endogenous LvNotch and overexpression of these constructs did not result in a detectable increase in endogenous LvNotch expression (see Fig. 1B,C; data not shown). Thus, thi ...
... The level of endogenous LvNotch protein in 30 embryos was found to be slightly below the detection limit of α-LvNotch pAbs for endogenous LvNotch and overexpression of these constructs did not result in a detectable increase in endogenous LvNotch expression (see Fig. 1B,C; data not shown). Thus, thi ...
Regulatory Mechanisms for Specification and Patterning of Plant
... (TF), LONESOME HIGHWAY, is a potential regulator of this vascular patterning ...
... (TF), LONESOME HIGHWAY, is a potential regulator of this vascular patterning ...
Inositol and Plant Cell Wall Polysaccharide Biogenesis
... the rMIO protein in bacteria. Their enzyme, a 32.7-kDa protein, lacked significant sequence to other known proteins. Native pig MIOase appears to complex with GlcUA reductase to produce L-gulonate, the second intermediate leading to AsA in AsA-synthesizing animals. The pathogenic yeast, Cryptococcus ...
... the rMIO protein in bacteria. Their enzyme, a 32.7-kDa protein, lacked significant sequence to other known proteins. Native pig MIOase appears to complex with GlcUA reductase to produce L-gulonate, the second intermediate leading to AsA in AsA-synthesizing animals. The pathogenic yeast, Cryptococcus ...
Activating the DNA damage checkpoint in a developmental context
... syncytial cycles delays not the entry into mitosis but the segregation of sister chromosomes during mitosis [7]. The mechanistic basis for this response remains to be understood. Syncytial cycles are followed by cellularization and maternal-to-zygotic transition of cell cycle control (MZT; similar t ...
... syncytial cycles delays not the entry into mitosis but the segregation of sister chromosomes during mitosis [7]. The mechanistic basis for this response remains to be understood. Syncytial cycles are followed by cellularization and maternal-to-zygotic transition of cell cycle control (MZT; similar t ...
Extracellular matrix
In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).