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Preview of “ZEISS Microscopy Online ...opy Basics | Objectives”
Preview of “ZEISS Microscopy Online ...opy Basics | Objectives”

... individual  internal  single-­element  lenses.  The  objective  also  has  a  hemispherical  front  lens  and a  meniscus  second  lens,  which  work  synchronously  to  assist  in  capturing  light  rays  at  high numerical  aperture  with  a  minimum  of  spherical  aberration.  Many  high  magnif ...
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... and a decrease in resolution when 800 nm or 1.3 µm light sources are used for OCT imaging of biological tissues. Figure 2 shows the calculated broadening of the autocorrelation function due to nonzero dispersion with Eq. (1). The horizontal axis shows OCT resolution which is determined by the sourc ...
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Problem Set 1 (due 2/21/06)

... modes of water are relatively small, and aqueous samples may be analyzed (water absorbs strongly in the IR). This allows analysis of a wide range of chemical and biological samples (even in vivo). Raman measures vibrational mode energies, but uses a visible wavelength light source. Optics and detect ...
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Microscopy



Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy.Optical and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. The development of microscopy revolutionized biology and remains an essential technique in the life and physical sciences.
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