
THEORY Geometrical optics, or ray optics, describes geometric
... where dimg = S2 is the distance associated with the image and is considered by convention to be negative if on the same side of the lens as the object and positive if on the opposite side of the lens. The focal length f is considered negative for concave lenses. Incoming parallel rays are focused by ...
... where dimg = S2 is the distance associated with the image and is considered by convention to be negative if on the same side of the lens as the object and positive if on the opposite side of the lens. The focal length f is considered negative for concave lenses. Incoming parallel rays are focused by ...
DIGITAL OFF-AXIS HOLOGRAPHIC MICROSCOPY: FROM CELLS
... Mach-Zehnder interferometer, because it offers flexibility in the geometrical arrangement [28]. For biological samples, which are transparent for visible wavelengths, the transmission geometry was chosen. One microscope objective is needed in the object beam, in order to magnify the investigated sam ...
... Mach-Zehnder interferometer, because it offers flexibility in the geometrical arrangement [28]. For biological samples, which are transparent for visible wavelengths, the transmission geometry was chosen. One microscope objective is needed in the object beam, in order to magnify the investigated sam ...
DIGITAL OFF-AXIS HOLOGRAPHIC MICROSCOPY: FROM CELLS
... Mach-Zehnder interferometer, because it offers flexibility in the geometrical arrangement [28]. For biological samples, which are transparent for visible wavelengths, the transmission geometry was chosen. One microscope objective is needed in the object beam, in order to magnify the investigated sam ...
... Mach-Zehnder interferometer, because it offers flexibility in the geometrical arrangement [28]. For biological samples, which are transparent for visible wavelengths, the transmission geometry was chosen. One microscope objective is needed in the object beam, in order to magnify the investigated sam ...
Research Directions
... While passing through the sample the two components experience different refractive indices n +: ...
... While passing through the sample the two components experience different refractive indices n +: ...
Plane Mirror Worksheet - Solutions
... Copy this distance (dOtop) to the other side of the mirror. It becomes (dItop). ...
... Copy this distance (dOtop) to the other side of the mirror. It becomes (dItop). ...
Chapter 10: Simple Harmonic Motion
... Chapter 38: Diffraction and Polarization For a single opening in a barrier, we might expect that a plane wave (light beam) would produce a bright spot the same size as the open However, what we actually see is a series of light and dark fringes similar the double-slit interference ...
... Chapter 38: Diffraction and Polarization For a single opening in a barrier, we might expect that a plane wave (light beam) would produce a bright spot the same size as the open However, what we actually see is a series of light and dark fringes similar the double-slit interference ...
Light Kit Student Concepts/Objectives per Lesson
... 2. Kit Objectives for this lesson: Observe and discuss the reflections of light from a white screen, a silvered (mirrored) surface and a half-silvered surface Compare an object with its image in a plane mirror Determine, through observations and measurements, that the image seen in a mirror appears ...
... 2. Kit Objectives for this lesson: Observe and discuss the reflections of light from a white screen, a silvered (mirrored) surface and a half-silvered surface Compare an object with its image in a plane mirror Determine, through observations and measurements, that the image seen in a mirror appears ...
EXPERIMENTAL TECHNIQUES
... configuration of the paths of the incident and reflected beams gives the reflected image a high level of illumination. This makes it easy to visualise the sample and provide a high contrast in the observation ...
... configuration of the paths of the incident and reflected beams gives the reflected image a high level of illumination. This makes it easy to visualise the sample and provide a high contrast in the observation ...
BioE 123 Teaching Material Stanford University
... Note that many lens shapes are spherical because these are the easiest surfaces to make. In order to make a sphere, two surfaces are ground together in rotation and both naturally become spherical. Spherical surfaces are “not thick enough” at the edges compared to the ideal curves pictured above and ...
... Note that many lens shapes are spherical because these are the easiest surfaces to make. In order to make a sphere, two surfaces are ground together in rotation and both naturally become spherical. Spherical surfaces are “not thick enough” at the edges compared to the ideal curves pictured above and ...
Image formation and optical transfer function in a course of
... We have also computed the image of a five-bar object. The dimensions of the bars are 0.52 nn and the period is 1.04 .tm. The frequency of this five-bar object in the units used in Eq.(2) is 0.702 for NAO.5 and &=O.365tm. Fig. 4 shows the images of the five-bar test given by a system without aberrat ...
... We have also computed the image of a five-bar object. The dimensions of the bars are 0.52 nn and the period is 1.04 .tm. The frequency of this five-bar object in the units used in Eq.(2) is 0.702 for NAO.5 and &=O.365tm. Fig. 4 shows the images of the five-bar test given by a system without aberrat ...
Wave Optics Theory and 3-D Deconvolution for the Light Field
... essentially a simultaneous tomographic imaging technique in which all N 2 projections are collected at once as pinhole views. Thus, successful deconvolution amounts to effectively fusing these low resolution views to create a high resolution volumetric reconstruction. How much resolution can be reco ...
... essentially a simultaneous tomographic imaging technique in which all N 2 projections are collected at once as pinhole views. Thus, successful deconvolution amounts to effectively fusing these low resolution views to create a high resolution volumetric reconstruction. How much resolution can be reco ...
L16
... not molecules) are placed in a strong magnetic field (~ 1 tesla), splitting of electronic energy levels takes place. The simplest splitting of one energy level results in three energy levels, one at a higher energy, another at a lower energy (two s satellite lines) and the third remains at the same ...
... not molecules) are placed in a strong magnetic field (~ 1 tesla), splitting of electronic energy levels takes place. The simplest splitting of one energy level results in three energy levels, one at a higher energy, another at a lower energy (two s satellite lines) and the third remains at the same ...
MLSystems Lab 1 - Fourier v4 - RIT
... produced when a diffraction grating is illuminated by coherent illumination. These coefficients, represented as terms in the harmonic decomposition of m(x) correspond to the discrete orders seen in above. The zeroth order (centered at u = 0) corresponds to the constant DC term A/2. At either side ar ...
... produced when a diffraction grating is illuminated by coherent illumination. These coefficients, represented as terms in the harmonic decomposition of m(x) correspond to the discrete orders seen in above. The zeroth order (centered at u = 0) corresponds to the constant DC term A/2. At either side ar ...
Optical Diffraction and Image Formation
... Fig. 12 The depth of field is the result of diffraction-limited resolution. ...
... Fig. 12 The depth of field is the result of diffraction-limited resolution. ...
Lecture 18 - Purdue Physics
... • Using ray diagrams, the is exactly the same distance behind the plane mirror as the object is in front of it. ...
... • Using ray diagrams, the is exactly the same distance behind the plane mirror as the object is in front of it. ...
Coherent Optical Information Systems
... round-trip distance between two grating eleniobate crystal by the interference pattern produced with two green to the incoherence of ments is equal to the illuminating wavelength, beams ( ⫽ 514 nm). The grating is illuminated from a different angle the source does not with infrared light (wavelengt ...
... round-trip distance between two grating eleniobate crystal by the interference pattern produced with two green to the incoherence of ments is equal to the illuminating wavelength, beams ( ⫽ 514 nm). The grating is illuminated from a different angle the source does not with infrared light (wavelengt ...
A tutorial for designing fundamental imaging systems
... (1) Lens, mirrors and prisms Lens, mirrors and prisms are most basic optical elements. These deviate rays and we can condense rays or rotate images. At first we decide what lens to use and calculate focal length or other basic features. (2) Image sensor ( Photo detector ) Image sensor transfers ligh ...
... (1) Lens, mirrors and prisms Lens, mirrors and prisms are most basic optical elements. These deviate rays and we can condense rays or rotate images. At first we decide what lens to use and calculate focal length or other basic features. (2) Image sensor ( Photo detector ) Image sensor transfers ligh ...
The Spectrophotometer
... rather than a simple line spectrum. In the sample absorption spectrum shown below, you see a broad peak over 200 nm wide in the blue to yellow portions of the visible portion of the spectrum. The reason for this is that the atoms in the hydrated ions vibrate back and forth with respect to each other ...
... rather than a simple line spectrum. In the sample absorption spectrum shown below, you see a broad peak over 200 nm wide in the blue to yellow portions of the visible portion of the spectrum. The reason for this is that the atoms in the hydrated ions vibrate back and forth with respect to each other ...
Wave optics theory and 3-D deconvolution
... The reconstruction technique we have developed is closely related to “computational superresolution” methods in the field of computer vision [3]. This signal processing technique combines multiple under-sampled and aliased images of a scene to recover an image with sub-pixel (or in our case, sub-len ...
... The reconstruction technique we have developed is closely related to “computational superresolution” methods in the field of computer vision [3]. This signal processing technique combines multiple under-sampled and aliased images of a scene to recover an image with sub-pixel (or in our case, sub-len ...
CCD-Based Instrumentation for Radiometric
... a billionth of a meter. Optical radiation lies between radio waves and x-rays on the spectrum, exhibiting a unique mix of ray, wave, and quantum properties. In optics radiometry is a set of techniques for measuring electromagnetic radiation including visible light. A radiometric technique which char ...
... a billionth of a meter. Optical radiation lies between radio waves and x-rays on the spectrum, exhibiting a unique mix of ray, wave, and quantum properties. In optics radiometry is a set of techniques for measuring electromagnetic radiation including visible light. A radiometric technique which char ...
Visible Wavelength Fiber Bragg Grating Arrays for
... As depicted in Fig. 1, a serial array of strong (R>90%), broadband (bandwidth>10nm) visible fiber Bragg gratings is used to map wavelength bins into time slots [3]. This design can resolve wavelengths to within 1-10 nanometers over a wide configurable spectral bandwidth. When a broadband pulse of li ...
... As depicted in Fig. 1, a serial array of strong (R>90%), broadband (bandwidth>10nm) visible fiber Bragg gratings is used to map wavelength bins into time slots [3]. This design can resolve wavelengths to within 1-10 nanometers over a wide configurable spectral bandwidth. When a broadband pulse of li ...
Polarization
... • The tiny particles in the atmosphere (dust, clumps of air molecules, microscopic water droplets) are better at scattering shorter wavelength blue light than the longer wavelength red light. • As sunlight passes through the atmosphere, the scattered blue light give the atmosphere an overall blue gl ...
... • The tiny particles in the atmosphere (dust, clumps of air molecules, microscopic water droplets) are better at scattering shorter wavelength blue light than the longer wavelength red light. • As sunlight passes through the atmosphere, the scattered blue light give the atmosphere an overall blue gl ...
Acousto-Optic Devices - Panasonic Industrial Devices
... the former is an acousto-optic light modulator, and using the latter is an acousto-optic light deflector and acoustooptic tunable flter. The acousto-optic light modulator can modulate laser beam intensity by means of amplitude modulation at a fixed frequency. The acousto-optic light deflector is cap ...
... the former is an acousto-optic light modulator, and using the latter is an acousto-optic light deflector and acoustooptic tunable flter. The acousto-optic light modulator can modulate laser beam intensity by means of amplitude modulation at a fixed frequency. The acousto-optic light deflector is cap ...
Microscopy

Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy.Optical and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. The development of microscopy revolutionized biology and remains an essential technique in the life and physical sciences.