Cloning of a T-Type Ca Channel Isoform in I n s u l i n
... In addition to the single amino acids that differ from 1G, the T-type Ca2+ channel gene derived from -cells contains three unique regions that differ from the amino acid sequence of 1G. These regions are located at the NH2-terminal amino acids (amino acids 1–34), intracellular loop LII–III (amino ac ...
... In addition to the single amino acids that differ from 1G, the T-type Ca2+ channel gene derived from -cells contains three unique regions that differ from the amino acid sequence of 1G. These regions are located at the NH2-terminal amino acids (amino acids 1–34), intracellular loop LII–III (amino ac ...
Natural Variation in Sensitivity to a Loss of Chloroplast Translation in
... Mutations that eliminate chloroplast translation in Arabidopsis (Arabidopsis thaliana) result in embryo lethality. The stage of embryo arrest, however, can be influenced by genetic background. To identify genes responsible for improved growth in the absence of chloroplast translation, we examined see ...
... Mutations that eliminate chloroplast translation in Arabidopsis (Arabidopsis thaliana) result in embryo lethality. The stage of embryo arrest, however, can be influenced by genetic background. To identify genes responsible for improved growth in the absence of chloroplast translation, we examined see ...
Role of Lipids and Fatty Acids in Stress Tolerance in Cyanobacteria
... reaction mechanism; they contribute to the stability of proteins, DNA and lipids. Water may have played a determinative role in the origin and evolution of the genetic code (Wolfenden et al. 1979, Potts 1994). Some desiccation-tolerant cells accumulate large amounts of either one or both of the disa ...
... reaction mechanism; they contribute to the stability of proteins, DNA and lipids. Water may have played a determinative role in the origin and evolution of the genetic code (Wolfenden et al. 1979, Potts 1994). Some desiccation-tolerant cells accumulate large amounts of either one or both of the disa ...
Lactose Intolerance
... of lactase. When an individual with this mutated gene has children with someone who lacks the mutated gene, half of their children are likely to develop lactose intolerance as adults. To restate, lactose intolerance was once normal in nearly all people, but a genetic mutation caused lactase over-pro ...
... of lactase. When an individual with this mutated gene has children with someone who lacks the mutated gene, half of their children are likely to develop lactose intolerance as adults. To restate, lactose intolerance was once normal in nearly all people, but a genetic mutation caused lactase over-pro ...
Rate variation during molecular evolution: creationism and the
... record are used to determine the number of amino acid changes per unit of time, the rate at which a molecular clock is “ticking”. Amino acid sequence comparisons for a specific protein can only be used as a molecular clock due to mutations in the gene coding for that protein. These mutations take pl ...
... record are used to determine the number of amino acid changes per unit of time, the rate at which a molecular clock is “ticking”. Amino acid sequence comparisons for a specific protein can only be used as a molecular clock due to mutations in the gene coding for that protein. These mutations take pl ...
Identification of Functional Connections Between Calmodulin and the Yeast Actin Cytoskeleton.
... Three independent libraries containing CMD1 random substitution mutations (i.e., altered sequences still capable of producing functional calmodulin) at positions 89–92 were used in this study. Plasmids containing these CMD1 mutations were recovered from yeast, amplified in E. coli, and transformed b ...
... Three independent libraries containing CMD1 random substitution mutations (i.e., altered sequences still capable of producing functional calmodulin) at positions 89–92 were used in this study. Plasmids containing these CMD1 mutations were recovered from yeast, amplified in E. coli, and transformed b ...
A newly discovered Anaerococcus strain responsible for axillary
... 2007). DNA amplification was conducted using the following method: preheating at 95 °C for 3 min; 30 cycles each of denaturation at 95 °C for 30 s, annealing at 50 °C for 30 s, extension at 72 °C for 1.5 min, and final extension at 72 °C for 10 min. The quantitative real-time PCR (qPCR) products wer ...
... 2007). DNA amplification was conducted using the following method: preheating at 95 °C for 3 min; 30 cycles each of denaturation at 95 °C for 30 s, annealing at 50 °C for 30 s, extension at 72 °C for 1.5 min, and final extension at 72 °C for 10 min. The quantitative real-time PCR (qPCR) products wer ...
Fig 1 - Centre for Biodiversity Genomics
... 39.0% in the Pseudoscorpiones (Table 1). As six orders were represented by a single family, Family Distances could only be determined for ten orders but these results showed four-fold variation with mean values for these orders ranging from 7.1% in Solifugae to 35.4% in Opiliones (Table 1, Fig 2). B ...
... 39.0% in the Pseudoscorpiones (Table 1). As six orders were represented by a single family, Family Distances could only be determined for ten orders but these results showed four-fold variation with mean values for these orders ranging from 7.1% in Solifugae to 35.4% in Opiliones (Table 1, Fig 2). B ...
The Inheritance of Penicillin Titre in Crosses between
... be broken up and the individual genes recombined additively with those selected in other lines to produce further increases in titre. Similar crosses between lines selected for linear growth rate have been .described by Simchen (1966) for monokaryons of SchizophylZum commune and by Papa (1970) for N ...
... be broken up and the individual genes recombined additively with those selected in other lines to produce further increases in titre. Similar crosses between lines selected for linear growth rate have been .described by Simchen (1966) for monokaryons of SchizophylZum commune and by Papa (1970) for N ...
1 - DORAS
... decenylamino)propane. Rhizobactin 1021 is structurally similar to other citrate based dihydroxamates, namely, aerobactin, arthrobactin and schizokinen. However, it primarily differs in that it contains an additional fatty acid residue. This may account for its amphiphilic properties. The genes encod ...
... decenylamino)propane. Rhizobactin 1021 is structurally similar to other citrate based dihydroxamates, namely, aerobactin, arthrobactin and schizokinen. However, it primarily differs in that it contains an additional fatty acid residue. This may account for its amphiphilic properties. The genes encod ...
Gluconacetobacter entanii sp. nov., isolated from submerged high
... 4637. As the two strains exhibited the same physiological properties, the results obtained for strain LTH 4560T were compared with the characteristics of the type strains of the acetic acid bacteria (Table 1). Remarkably, the isolates did not grow in AE broth without acetic acid. Within the genus Gl ...
... 4637. As the two strains exhibited the same physiological properties, the results obtained for strain LTH 4560T were compared with the characteristics of the type strains of the acetic acid bacteria (Table 1). Remarkably, the isolates did not grow in AE broth without acetic acid. Within the genus Gl ...
Preparation of Human Metabolites of Propranolol Using Laboratory-Evolved Bacterial Cytochromes P450
... variable expression levels make their use in preparative-scale metabolite synthesis impractical. Some human enzymes can also be obtained by expression in recombinant hosts. As membrane-bound, multi-protein systems, however, they commonly misfold and aggregate or are not expressed in active form. Met ...
... variable expression levels make their use in preparative-scale metabolite synthesis impractical. Some human enzymes can also be obtained by expression in recombinant hosts. As membrane-bound, multi-protein systems, however, they commonly misfold and aggregate or are not expressed in active form. Met ...
The nonenzymatic subunit of pseutarin C, a
... size 2 kb and 700 bp; with IP3/A2R a 2-kb fragment was obtained and for Nter/A2R we got a 600-bp product (data not shown). The PCR products were either gel-eluted or purified directly and their sequences were determined. The deduced amino acid sequences showed similarity (⬃60%) to the A2 and A3 doma ...
... size 2 kb and 700 bp; with IP3/A2R a 2-kb fragment was obtained and for Nter/A2R we got a 600-bp product (data not shown). The PCR products were either gel-eluted or purified directly and their sequences were determined. The deduced amino acid sequences showed similarity (⬃60%) to the A2 and A3 doma ...
Succinate Dehydrogenase of Saccharomyces cerevisiae
... Current Knowledge and New Perspectives Dorota Kregiel Additional information is available at the end of the chapter http://dx.doi.org/10.5772/48413 ...
... Current Knowledge and New Perspectives Dorota Kregiel Additional information is available at the end of the chapter http://dx.doi.org/10.5772/48413 ...
The Ethylene Signal Transduction Pathway in Plants
... ACC oxidase is constitutively present in most tissues, but its synthesis is increased during fruit ripening in tomato. The genes that encode A C C synthase and A C C oxidase have been cloned and characterized from many plant species (5, 6). A C C synthase is encoded by multigene families in all spec ...
... ACC oxidase is constitutively present in most tissues, but its synthesis is increased during fruit ripening in tomato. The genes that encode A C C synthase and A C C oxidase have been cloned and characterized from many plant species (5, 6). A C C synthase is encoded by multigene families in all spec ...
Ribosome readthrough
... The efficiency of translation termination (and the occurrence of readthrough) can vary depending on many factors 1) The efficiency of termination differs between normal stop codons and premature termination codons 2) Aminoglycosides can decrease the fidelity of translation, causing higher frequenci ...
... The efficiency of translation termination (and the occurrence of readthrough) can vary depending on many factors 1) The efficiency of termination differs between normal stop codons and premature termination codons 2) Aminoglycosides can decrease the fidelity of translation, causing higher frequenci ...
Lecture 4
... • 2000 first draft of genome published • More than 200,000 expressed sequence tags available • Large scale transposon mutagenesis • Flybase: computer database and resource ...
... • 2000 first draft of genome published • More than 200,000 expressed sequence tags available • Large scale transposon mutagenesis • Flybase: computer database and resource ...
Introduction to Carbohydrates
... The digestion of proteins begins in the stomach, which secretes gastric juice—a unique solution containing hydrochloric acid and the proenzyme, pepsinogen. Hydrochloric acid: Stomach acid is too dilute (pH 2–3) to hydrolyze proteins. The acid functions instead to kill some bacteria and to denatu ...
... The digestion of proteins begins in the stomach, which secretes gastric juice—a unique solution containing hydrochloric acid and the proenzyme, pepsinogen. Hydrochloric acid: Stomach acid is too dilute (pH 2–3) to hydrolyze proteins. The acid functions instead to kill some bacteria and to denatu ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... identification of 65 significantly related proteins. An initial alignment and phylogenetic tree constructed from these sequences (not shown) confirmed that this superfamily of proteins, the aldo-keto reductases, is highly divergent, with multiple representatives in diverse proand eukaryotes. However ...
... identification of 65 significantly related proteins. An initial alignment and phylogenetic tree constructed from these sequences (not shown) confirmed that this superfamily of proteins, the aldo-keto reductases, is highly divergent, with multiple representatives in diverse proand eukaryotes. However ...
The Rad50 Signature Motif: Essential to ATP Binding and
... The Mre11 protein contains a nuclease domain that is highly conserved and related to the lambda phosphatase family of phosphoesterases. The crystal structure of an Mre11 homolog from Pyrococcus furiosus shows five conserved motifs within the N-terminal half of Mre11 that coordinate two manganese ion ...
... The Mre11 protein contains a nuclease domain that is highly conserved and related to the lambda phosphatase family of phosphoesterases. The crystal structure of an Mre11 homolog from Pyrococcus furiosus shows five conserved motifs within the N-terminal half of Mre11 that coordinate two manganese ion ...
Krogh, A., Brown, M., Mian, I.S., Sjolander, K., and Haussler, D. Hidden Markov Models in Computational Biology. J. Mol. Biol. , 235. pp. 1501-1531, 1994.
... domains. These methods are demonstrated the on globin family, the protein kinase catalytic domain, and the EF-hand calcium binding motif. In each case the parameters of an HMM are estimated from a training set of unaligned sequences. Afterthe HMM is built, itis used to obtain a multiple alignment of ...
... domains. These methods are demonstrated the on globin family, the protein kinase catalytic domain, and the EF-hand calcium binding motif. In each case the parameters of an HMM are estimated from a training set of unaligned sequences. Afterthe HMM is built, itis used to obtain a multiple alignment of ...
Volume 46 - Tomato Genetics Cooperative
... (Harley and Smith, 1983; Remy et al., 1994). Despite their extensive co-relationship, and although the fungal partner cannot yet be cultured in the absence of a plant, many plant species are not obligate symbionts. Thus, at least some of the plant genes that are required for successful establishment ...
... (Harley and Smith, 1983; Remy et al., 1994). Despite their extensive co-relationship, and although the fungal partner cannot yet be cultured in the absence of a plant, many plant species are not obligate symbionts. Thus, at least some of the plant genes that are required for successful establishment ...
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... Bowman, 2004; Mallory et al., 2004). The phb rev double, phb phv rev triple and other mutant combinations involving athb8 and athb15 lack the SAM and, in most severe cases, fail to establish bilateral symmetry (Emery et al., 2003; Prigge et al., 2005). Defects in the pattern of PIN1 expression are e ...
... Bowman, 2004; Mallory et al., 2004). The phb rev double, phb phv rev triple and other mutant combinations involving athb8 and athb15 lack the SAM and, in most severe cases, fail to establish bilateral symmetry (Emery et al., 2003; Prigge et al., 2005). Defects in the pattern of PIN1 expression are e ...
