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Identification of a Cis-Acting Element of ART1, a
Identification of a Cis-Acting Element of ART1, a

The enhancement of ribosomal transcription by the recycling of RNA
The enhancement of ribosomal transcription by the recycling of RNA

... pX1108c in the same orientation as the wild type terminator, (pXUGlc), a second with two terminators inserted tandemly in this orientation, (pXHG2c) and a third with the terminator inserted into, pXl^ESlc in the corresponding position but the opposite orientation, (pXUGllc). Each construct was micro ...
PersPecTIves - Ralf Sommer
PersPecTIves - Ralf Sommer

Porphyrins (Structure of Porphyrins)
Porphyrins (Structure of Porphyrins)

The somatic-visceral subdivision of the embryonic mesoderm is
The somatic-visceral subdivision of the embryonic mesoderm is

... balancer chromosome. Mutants also possess an expanded cephalic furrow. The details of the 2xPE-sna fusion gene are described by Ip et al. (1994). It includes the entire sna genomic coding region and 1.6 kb of the 5′ flanking sequences. This genomic fragment was placed downstream of two tandem copies ...
Mid-term PowerPoint Presentation
Mid-term PowerPoint Presentation

... Tetrahymena vesicles live longer than free floating E. coli? • My objective is to show that E. coli O157:H7 survive longer in ciliate vesicles than do E. coli that are freefloating in the environment. • I will accomplish this goal by feeding Tetrahymena ciliates E. coli O157:H7 and then observing th ...
Amino Acids and Peptides
Amino Acids and Peptides

... in turn form structures called proteins. The process of such formation from an mRNA template is known as translation, which is part of protein synthesis.  Phenylalanine is one of the standard amino acids. ...
Modern Genetics
Modern Genetics

... Results in offspring that have characteristics of both parents. Organisms are more genetically different and are therefore likely to be stronger and healthier than inbred organisms. This is especially true in hybrid plant varieties. ...
PDF
PDF

... “Ferrovum” strain JA12 consists of 1.99 Mbp and contains 2,001 open reading frames (ORF) of which 1,960 were predicted to be protein-coding genes. The general genome features are summarised in Table 1. The majority of the protein-coding genes (1,462) were assigned to COG classifications (S1 Table). ...
Modern Genetics
Modern Genetics

... Results in offspring that have characteristics of both parents. Organisms are more genetically different and are therefore likely to be stronger and healthier than inbred organisms. This is especially true in hybrid plant varieties. ...
Transacylation as a chain-termination mechanism in fatty acid
Transacylation as a chain-termination mechanism in fatty acid

... We therefore suggest that this enzyme is also involved in the termination of medium-chain fatty acid synthesis by analogy with the malonyl- and palmitoyl-transacylase in yeast fatty acid synthetase (Engeser et al., 1979). However, the final solution of this problem will have to await identification ...
1 Supplemental Table 1. FACS-isolated, SSEA-4
1 Supplemental Table 1. FACS-isolated, SSEA-4

... -mutations in this gene are associated with cancer. -may play a role in the development and normal function of the ovaries, defects in this gene have been linked to premature ovarian failure 2, -could be involved in oogenesis. -this gene encodes an orphan nuclear receptor which is a member of the nu ...
TRIPTYCHON and CAPRICE mediate lateral inhibition during
TRIPTYCHON and CAPRICE mediate lateral inhibition during

... trichome development, GL2 governs early differentiation processes. GL2 is expressed exclusively in non-hair cells and suppresses the formation of root hairs (Masucci et al., 1996). Its expression is controlled by WER and TTG1. The CAPRICE (CPC) gene, encoding a MYB-related transcription factor lacki ...
Design and Synthesis of RGD Mimetics as Potent Inhibitors of
Design and Synthesis of RGD Mimetics as Potent Inhibitors of

... function of bioactive peptides. Known as peptidomimetics, these compounds have many advantages over their parent peptides.4 Peptides are characterized by their rapid digestion by proteolytic enzymes, low absorption through intestinal walls, and potential immunogenic reactions. 5 The proper design of ...
Clinical Features, and Nucleotide Changes in Three Families with
Clinical Features, and Nucleotide Changes in Three Families with

... approved by the Institutional Review Board at Okayama University Medical School, and written consent was obtained from each patient or a parent when the patient was below age 15. All of the procedures conformed to the Declaration of Helsinki.   Briefly, peripheral leukocytes were isolated from 10 ml ...
Chapter 3: Child Development
Chapter 3: Child Development

... Dominant: The gene’s feature will appear each time the gene is present  Recessive: The gene’s feature will appear only if it is paired with another recessive gene ...
Dia 1
Dia 1

... using linkage analysis • cloning through physical mapping • genomic sequence 250 kb coding sequence 6.5 kb • almost exclusively expressed in epithelial cells • 3bp deletion in exon 10 in 70% in CF patients • CFTR = cystic fibrosis conductance regulator ...
Chapter 3: Child Development
Chapter 3: Child Development

... Dominant: The gene’s feature will appear each time the gene is present  Recessive: The gene’s feature will appear only if it is paired with another recessive gene ...
Classification and domain analysis of protein
Classification and domain analysis of protein

... three GO annotation terms mentioned earlier. The list was further subjected to manual curation to exclude any regulatory proteins that binds to a kinase. The final list of proteins fulfilling the above curation process was classified as true protein kinases and used in this study. All other proteins ...
Chapter 3: Child Development
Chapter 3: Child Development

... Dominant: The gene’s feature will appear each time the gene is present  Recessive: The gene’s feature will appear only if it is paired with another recessive gene ...
Julio`s MCB - Digital Access to Scholarship at Harvard
Julio`s MCB - Digital Access to Scholarship at Harvard

... erythroid transcription factor GATA-1. We identified the CRMs that faithfully drive the expression of Mfrn1 during blood and heart development and Mfrn2 ubiquitously. Through in vivo analyses of the Mfrn-CRMs in zebrafish and mouse, we demonstrate their functional and evolutionary conservation. Usin ...
Staphylococcus haemolyticus lipase
Staphylococcus haemolyticus lipase

Risk assessment - Office of the Gene Technology Regulator
Risk assessment - Office of the Gene Technology Regulator

... that do not lead to an adverse outcome, or could not reasonably occur, do not advance in the risk assessment process. Sixteen events were identified and assessed whereby the release of the GM sugarcane lines might give rise to harm to people or the environment. These 16 events included consideration ...
Quantification of Protein Levels in Single Living
Quantification of Protein Levels in Single Living

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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