chapter 1: exploring life
... 5.Distinguish between peripheral and integral membrane proteins. 6.List six major functions of membrane proteins. 7.Explain the role of membrane carbohydrates in cell-cell recognition. 8.Explain how hydrophobic molecules cross cell membranes. 9.Distinguish between channel proteins and carrier protei ...
... 5.Distinguish between peripheral and integral membrane proteins. 6.List six major functions of membrane proteins. 7.Explain the role of membrane carbohydrates in cell-cell recognition. 8.Explain how hydrophobic molecules cross cell membranes. 9.Distinguish between channel proteins and carrier protei ...
The Effect of Horizontal Gene Transfer on the Dynamics of
... possible that these are the strains that were accidentally generated under the experimental conditions that the plasmids were being studied in the experiment which they were originally discovered by Lederberg(Tatum and Lederberg 1947). Consequently, conjugation for the K12 strain occurs much faster ...
... possible that these are the strains that were accidentally generated under the experimental conditions that the plasmids were being studied in the experiment which they were originally discovered by Lederberg(Tatum and Lederberg 1947). Consequently, conjugation for the K12 strain occurs much faster ...
Visualizing expression patterns of Shh and Foxf1 genes
... notochord, floor plate of the neural tube and polarizing region of the limbs [7]. It is involved in dorso-ventral foregut patterning [7, 8], presumably through notochord localization. The later expression of Shh in lung buds suggests that it plays a role in the morphogenesis of various epithelial ap ...
... notochord, floor plate of the neural tube and polarizing region of the limbs [7]. It is involved in dorso-ventral foregut patterning [7, 8], presumably through notochord localization. The later expression of Shh in lung buds suggests that it plays a role in the morphogenesis of various epithelial ap ...
A viability-linked metagenomic analysis of
... thought to be strongly influenced by the human microbiome. The cleanroom itself has previously been posited as representing an extreme environment [17], characterized by rigorous cleaning and bioburden control regimens, controlled humidity (45 ± 5 %) and temperature (25° C), and a paucity of availab ...
... thought to be strongly influenced by the human microbiome. The cleanroom itself has previously been posited as representing an extreme environment [17], characterized by rigorous cleaning and bioburden control regimens, controlled humidity (45 ± 5 %) and temperature (25° C), and a paucity of availab ...
PAX6 mRNA Transcript Analysis in Various Ocular/Non
... Generally reverse transcription reactions are performed with one of the primers of randon hexamers or oligonucleotides (Oligo dTs) or an olignucleotide that can hybridize with the specific RNA under the study. However, in this study two types of first strand cDNA (Complementary DNA) conversion were ...
... Generally reverse transcription reactions are performed with one of the primers of randon hexamers or oligonucleotides (Oligo dTs) or an olignucleotide that can hybridize with the specific RNA under the study. However, in this study two types of first strand cDNA (Complementary DNA) conversion were ...
Probability-Based Scoring Function as a Software
... School of Information Technology, King Mongkut's University of Technology Thonburi, 126 Pracha-U-Thit Rd., Bangmod, Thungkru, Bangkok 10140, Thailand; 2BEC Unit, KMUTT-Bangkhuntien, 83 Moo 8, Thakham, Bangkhuntien, Bangkok 10150, Thailand; 3School of Bioresources and Technology, King Mongkut's Unive ...
... School of Information Technology, King Mongkut's University of Technology Thonburi, 126 Pracha-U-Thit Rd., Bangmod, Thungkru, Bangkok 10140, Thailand; 2BEC Unit, KMUTT-Bangkhuntien, 83 Moo 8, Thakham, Bangkhuntien, Bangkok 10150, Thailand; 3School of Bioresources and Technology, King Mongkut's Unive ...
Rhizobium Nodulation Protein NodC Is an Important Determinant of
... in the presence of [1-14C]GlcN, LCOs were extracted with butanol and quantitatively analyzed by using a TLC system in which the LCOs of R. leguminosarum bv. viciae are separated on the basis of the lengths of their oligosaccharide backbones (11). Expression of R. leguminosarum bv. viciae nodC or R. ...
... in the presence of [1-14C]GlcN, LCOs were extracted with butanol and quantitatively analyzed by using a TLC system in which the LCOs of R. leguminosarum bv. viciae are separated on the basis of the lengths of their oligosaccharide backbones (11). Expression of R. leguminosarum bv. viciae nodC or R. ...
RELIC – A bioinformatics server for combinatorial
... the binding partner being sought may not be in the library, only a low number of conservatively close sequences. Affinity selection of a combinatorial peptide library screen may therefore generate a group of closely related sequences which are functional homologs, but may contain no obvious consensu ...
... the binding partner being sought may not be in the library, only a low number of conservatively close sequences. Affinity selection of a combinatorial peptide library screen may therefore generate a group of closely related sequences which are functional homologs, but may contain no obvious consensu ...
julian huxley: developmental genetics and the theory of evolution
... Several historians of science still believe that within his scientific experience, Huxley had made no genetic investigation, and that he and R. Goldschmidt were heretics, or even committed a crime, since they had deviated from a pure Morganistic “transmission genetics” stance. While it is known that ...
... Several historians of science still believe that within his scientific experience, Huxley had made no genetic investigation, and that he and R. Goldschmidt were heretics, or even committed a crime, since they had deviated from a pure Morganistic “transmission genetics” stance. While it is known that ...
View PDF - G3: Genes | Genomes | Genetics
... cross was 238, with a slight bias for progeny carrying the deficiency chromosome (t-test, P , 0.001). However, the numbers varied widely among crosses due to variable mating efficiency. A total of 72.5% and 52.4% of crosses produced a minimum of 50 and 100 deficiencycarrying females, respectively, summ ...
... cross was 238, with a slight bias for progeny carrying the deficiency chromosome (t-test, P , 0.001). However, the numbers varied widely among crosses due to variable mating efficiency. A total of 72.5% and 52.4% of crosses produced a minimum of 50 and 100 deficiencycarrying females, respectively, summ ...
Relationship among phenotypic plasticity
... isogenic individuals is distributed (Oosawa 1975; Spudich and Koshland 1976). Still, too large variability in the phenotype relevant to fitness should be harmful to the survival of the organisms. Phenotype that is concerned with fitness is expected to keep some robustness against such stochasticity in ...
... isogenic individuals is distributed (Oosawa 1975; Spudich and Koshland 1976). Still, too large variability in the phenotype relevant to fitness should be harmful to the survival of the organisms. Phenotype that is concerned with fitness is expected to keep some robustness against such stochasticity in ...
Taxonomic Study of Anaerobic, Gram-Negative, Rod
... described. All of the strains contained directly cross-linked meso-diaminopimelic acid-containing peptidoglycan and in addition the diamine cadaverine or (rarely) putrescine. The diamine was covalently linked to the a-carboxyl group of D-glutamic acid in the peptide subunit of peptidoglycan. Lipid F ...
... described. All of the strains contained directly cross-linked meso-diaminopimelic acid-containing peptidoglycan and in addition the diamine cadaverine or (rarely) putrescine. The diamine was covalently linked to the a-carboxyl group of D-glutamic acid in the peptide subunit of peptidoglycan. Lipid F ...
Severe Combined Immunodeficiency (SCID)
... • There are several types of SCID. • The most common form is caused by a mutation in the SCIDX1 gene located on the X chromosome. ...
... • There are several types of SCID. • The most common form is caused by a mutation in the SCIDX1 gene located on the X chromosome. ...
Restriction fragment length polymorphism (RFLP) of exon 2 of the
... present in five American herds studied by these authors, is identical to cattle pattern m (111, 69, and 104 bp) with an additional restriction site (Fig. 2). We were not able to assess correspondence of all DRB3 alleles found in European bison with those previously found in cattle, because the major ...
... present in five American herds studied by these authors, is identical to cattle pattern m (111, 69, and 104 bp) with an additional restriction site (Fig. 2). We were not able to assess correspondence of all DRB3 alleles found in European bison with those previously found in cattle, because the major ...
Isolation of COV1, a gene involved in the regulation of vascular
... that are not connected to the existing vascular tissue are harder to reconcile with the canalisation hypothesis (Carland et al., 1999; Deyholos et al., 2000; Koizumi et al., 2000). Some of these mutants have normal responses to auxin and may be explained more easily by the diffusion-reaction pre-pat ...
... that are not connected to the existing vascular tissue are harder to reconcile with the canalisation hypothesis (Carland et al., 1999; Deyholos et al., 2000; Koizumi et al., 2000). Some of these mutants have normal responses to auxin and may be explained more easily by the diffusion-reaction pre-pat ...
SHY2/IAA3 regulates root development
... 56°C (1 minute), 72°C (2 minutes) followed by 5 minutes at 72°C. For shy2-2, the amplified genomic DNA fragments were cloned into pBluescript SK− (PstI/BamHI) by standard restriction enzyme digestion and ligation protocols and sequenced by standard methods. For shy2-3, shy2-21, shy2-22, shy2-23 and ...
... 56°C (1 minute), 72°C (2 minutes) followed by 5 minutes at 72°C. For shy2-2, the amplified genomic DNA fragments were cloned into pBluescript SK− (PstI/BamHI) by standard restriction enzyme digestion and ligation protocols and sequenced by standard methods. For shy2-3, shy2-21, shy2-22, shy2-23 and ...
Genetic Analysis of Apomixis
... present and to help create a map of the specific genomic region associated with apomixis. The problem with this technique, however, is that there are few traits specifically linked to apomixis that can be used as markers. Sherwood (2001) states that, “apomictic genes have no known effects on plant c ...
... present and to help create a map of the specific genomic region associated with apomixis. The problem with this technique, however, is that there are few traits specifically linked to apomixis that can be used as markers. Sherwood (2001) states that, “apomictic genes have no known effects on plant c ...
Analysis of the genetic potential of the spongederived fungus
... Ircinia fasciculata in the course of a research program focused on the discovery and characterization of novel natural products. This led to the isolation and characterisation of two novel and structurally highly similar polyketides: sorbicillactone A and sorbicillactone B. Consistent with their str ...
... Ircinia fasciculata in the course of a research program focused on the discovery and characterization of novel natural products. This led to the isolation and characterisation of two novel and structurally highly similar polyketides: sorbicillactone A and sorbicillactone B. Consistent with their str ...
Natural rules for Arabidopsis thaliana
... still remain unknown. This study, based on confirmed sequence data, systematically analyzed all expressed genes on Arabidopsis thaliana chromosome IV to quantitatively explore the natural splicing rules. The results indicated that defining Arabidopsis thaliana pre-mRNA splicing sites required a comb ...
... still remain unknown. This study, based on confirmed sequence data, systematically analyzed all expressed genes on Arabidopsis thaliana chromosome IV to quantitatively explore the natural splicing rules. The results indicated that defining Arabidopsis thaliana pre-mRNA splicing sites required a comb ...
Complex Lipids from Pathogenic Microorganisms
... multivesicular body/multivesicular tubule network. Stage specificity may reflect changes in membrane structure ...
... multivesicular body/multivesicular tubule network. Stage specificity may reflect changes in membrane structure ...
Couchioplanes caeruleus - International Journal of Systematic and
... DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate sequence templates, a PCR was performed twice. First, 16s rRNA genes were amplified by a PCR by using a standar ...
... DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate sequence templates, a PCR was performed twice. First, 16s rRNA genes were amplified by a PCR by using a standar ...
Predictable Alteration of Sequence Recognition by RNA
... (B) REMSA showing binding of CLB19 and the variants P2[ND] and P2[TN] to Cy5-labeled clpP and fluorescein-labeled clpP-9C or clpP-9A RNA oligonucleotides. Protein concentrations varied from 87.5 to 700 nM. Labeled oligonucleotides were at 750 pM. Each reaction was done in triplicate. (C) Log(relative ...
... (B) REMSA showing binding of CLB19 and the variants P2[ND] and P2[TN] to Cy5-labeled clpP and fluorescein-labeled clpP-9C or clpP-9A RNA oligonucleotides. Protein concentrations varied from 87.5 to 700 nM. Labeled oligonucleotides were at 750 pM. Each reaction was done in triplicate. (C) Log(relative ...
Table 3 - HAL Descartes
... QMF-PCR has been shown to be a sensitive method for the detection of gene dosage anomalies, and has been successfully used in our laboratory to characterize deletions and duplications within several genes 59; 60. We adapted the protocol previously described 61; 59 to screen for SOX10 gene deletions. ...
... QMF-PCR has been shown to be a sensitive method for the detection of gene dosage anomalies, and has been successfully used in our laboratory to characterize deletions and duplications within several genes 59; 60. We adapted the protocol previously described 61; 59 to screen for SOX10 gene deletions. ...
The American University in Cairo School of Science and Engineering
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...