Predictable Alteration of Sequence Recognition by RNA
... (B) REMSA showing binding of CLB19 and the variants P2[ND] and P2[TN] to Cy5-labeled clpP and fluorescein-labeled clpP-9C or clpP-9A RNA oligonucleotides. Protein concentrations varied from 87.5 to 700 nM. Labeled oligonucleotides were at 750 pM. Each reaction was done in triplicate. (C) Log(relative ...
... (B) REMSA showing binding of CLB19 and the variants P2[ND] and P2[TN] to Cy5-labeled clpP and fluorescein-labeled clpP-9C or clpP-9A RNA oligonucleotides. Protein concentrations varied from 87.5 to 700 nM. Labeled oligonucleotides were at 750 pM. Each reaction was done in triplicate. (C) Log(relative ...
Water in plants and animals
... Know that the position of a gene on a chromosome can be located using a gene probe. Know that genes can be transferred from one organism to another in the process of genetic engineering (particularly from human to bacteria). Know examples of human proteins which can be manufactured by bacteria as a ...
... Know that the position of a gene on a chromosome can be located using a gene probe. Know that genes can be transferred from one organism to another in the process of genetic engineering (particularly from human to bacteria). Know examples of human proteins which can be manufactured by bacteria as a ...
The American University in Cairo School of Science and Engineering
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
... variable, inhibition of the SHV activity by p-chloromercuribenzoate is substrate-related; and varies depending on the substrate used for the assay; however, studies never confirmed the validity of this hypothesis (Paterson and Bonomo, 2005). 7KH6+9ȕ-lactamase family originally was found in Klebsi ...
Couchioplanes caeruleus - International Journal of Systematic and
... DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate sequence templates, a PCR was performed twice. First, 16s rRNA genes were amplified by a PCR by using a standar ...
... DNA-DNA hybridization. DNA-DNA relatedness was measured fluorometrically by the method of Ezaki et al. (3), using biotinylated DNA. PCR and sequencing of the products. In order to generate sequence templates, a PCR was performed twice. First, 16s rRNA genes were amplified by a PCR by using a standar ...
Seminario Glúcidos 3 y lípidos 1. Comente los mecanismos de
... Schneider, and Pallade (5, 6) was used for the preparation of the particulate fractions of rat liver. Normal adult albino rats of Sprague-Dawley stock were used throughout this study. The animals were killed by decapitation and exsanguinated. The livers were quickly removed and chilled in cracked ic ...
... Schneider, and Pallade (5, 6) was used for the preparation of the particulate fractions of rat liver. Normal adult albino rats of Sprague-Dawley stock were used throughout this study. The animals were killed by decapitation and exsanguinated. The livers were quickly removed and chilled in cracked ic ...
Detection of Avian Influenza-DNA Hybridization
... analyte and its corresponding receptor. This method is also applicable in situ because hybridization kinetics can be detected immediately on the sensor surface. Among various SPR measurement schemes, SPR imaging systems have been explored extensively due to their potential for use in high-throughput ...
... analyte and its corresponding receptor. This method is also applicable in situ because hybridization kinetics can be detected immediately on the sensor surface. Among various SPR measurement schemes, SPR imaging systems have been explored extensively due to their potential for use in high-throughput ...
DNA Base Composition, DNA-DNA Homology and Long
... group D streptococci. Journal of General Microbiology 129, 1423-1432. GARVIE,E. I. (1976). Hybridization between the deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria. International Journal of Systematic Bacteriology 26, 1 16- 122. GARVIE,E. I. (1978). Streptococcus r ...
... group D streptococci. Journal of General Microbiology 129, 1423-1432. GARVIE,E. I. (1976). Hybridization between the deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria. International Journal of Systematic Bacteriology 26, 1 16- 122. GARVIE,E. I. (1978). Streptococcus r ...
DNA Base Composition, DNA-DNA Homology and Long
... group D streptococci. Journal of General Microbiology 129, 1423-1432. GARVIE,E. I. (1976). Hybridization between the deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria. International Journal of Systematic Bacteriology 26, 1 16- 122. GARVIE,E. I. (1978). Streptococcus r ...
... group D streptococci. Journal of General Microbiology 129, 1423-1432. GARVIE,E. I. (1976). Hybridization between the deoxyribonucleic acids of some strains of heterofermentative lactic acid bacteria. International Journal of Systematic Bacteriology 26, 1 16- 122. GARVIE,E. I. (1978). Streptococcus r ...
21. Toshihiko Okamotofi`1 Yo Isogai,$2 and T6111 K0izumi*1 :
... system have been published (Lam et al. 1996; McAllister et al. 2012; Xu et al. 2012). Briefly, NO3− is reduced to NO2− by nitrate reductase (NR) in the cytoplasm (Lea et al. 2006) and further reduced to NH4+ by nitrite reductase (NiR) in the plastid/chloroplasts (Takahashi et al. 2001). The NH4+ is ...
... system have been published (Lam et al. 1996; McAllister et al. 2012; Xu et al. 2012). Briefly, NO3− is reduced to NO2− by nitrate reductase (NR) in the cytoplasm (Lea et al. 2006) and further reduced to NH4+ by nitrite reductase (NiR) in the plastid/chloroplasts (Takahashi et al. 2001). The NH4+ is ...
UNUSUAL FLORAL ORGANS Controls Meristem ldentity and Organ
... and Table 1). Unlike the wild type, the meristems of all Ufo primary and coflorescence shoots terminate in a structure resembling either a normal pistil or fused sepal-carpel organs (Figure 3M). The number of nodes bearing flowers produced before termination in Ufo-1 is approximately the same as the ...
... and Table 1). Unlike the wild type, the meristems of all Ufo primary and coflorescence shoots terminate in a structure resembling either a normal pistil or fused sepal-carpel organs (Figure 3M). The number of nodes bearing flowers produced before termination in Ufo-1 is approximately the same as the ...
SPT4, a gene important for tr
... Southern hybridization analysis (Southern 1975) confirmed that the wild-type SPT4 allele had been replaced by spt4A 1 . : URA3. RNA preparation and analysis. Cells used for preparation of RNA were grown at 25°C in SD medium supplemented with the necessary requirements to a density of 1-2 x 107 cells ...
... Southern hybridization analysis (Southern 1975) confirmed that the wild-type SPT4 allele had been replaced by spt4A 1 . : URA3. RNA preparation and analysis. Cells used for preparation of RNA were grown at 25°C in SD medium supplemented with the necessary requirements to a density of 1-2 x 107 cells ...
Myrick LK, Nakamoto-Kinoshita M, Lindor NM, Warren ST , et al. Fragile X syndrome due to a missense mutation. European Journal of Human Genetics (2014) 22, 1185-1189.
... Figure 2 Functional analysis of mutant G266E-FMRP. (a) Constitutive AMPA receptor assay showing that G266E-FMRP is unable to rescue exaggerated AMPA receptor internalization in KO neurons. Hippocampal neurons from Fmr1 KO mice were cultured for 18 days, infected with either WT or G266EFMRP, and the ...
... Figure 2 Functional analysis of mutant G266E-FMRP. (a) Constitutive AMPA receptor assay showing that G266E-FMRP is unable to rescue exaggerated AMPA receptor internalization in KO neurons. Hippocampal neurons from Fmr1 KO mice were cultured for 18 days, infected with either WT or G266EFMRP, and the ...
Release Notes for Genomes Processed Using Complete Genomics
... 11. For a few transcripts in which alignment information cannot be parsed, impact field in gene-[ASM-ID].tsv.bz2 file will be annotated with “UNKNOWN-TR”. 12. Predicted genes without stop codon are not parsed correctly, leading to annotation of the variant with “UNKNOWN-TR” in the impact field in ge ...
... 11. For a few transcripts in which alignment information cannot be parsed, impact field in gene-[ASM-ID].tsv.bz2 file will be annotated with “UNKNOWN-TR”. 12. Predicted genes without stop codon are not parsed correctly, leading to annotation of the variant with “UNKNOWN-TR” in the impact field in ge ...
Phylogenetic analysis of three complete gap
... from the zebrafish genome, some are found as single relatives, and others are found in multiple copies. In addition, the zebrafish has 14 apparently novel connexins, several of which arose by recent tandem duplication events. This analysis provides evidence that the connexin gene family is increasin ...
... from the zebrafish genome, some are found as single relatives, and others are found in multiple copies. In addition, the zebrafish has 14 apparently novel connexins, several of which arose by recent tandem duplication events. This analysis provides evidence that the connexin gene family is increasin ...
Sickle Cell Disease and Hemoglobinopathy Screening in Pregnancy
... decreased (β+) or absent (β0 ) β protein product • Usually clinically asymptomatic • CBC often shows elevated RBC number with decreased MCV and/or MCH, in the absence of iron deficiency • Mean values are significantly different for those with β+ trait versus β0 trait, however there is overlap • Diag ...
... decreased (β+) or absent (β0 ) β protein product • Usually clinically asymptomatic • CBC often shows elevated RBC number with decreased MCV and/or MCH, in the absence of iron deficiency • Mean values are significantly different for those with β+ trait versus β0 trait, however there is overlap • Diag ...
Evolutionary History of Free-Swimming and
... template. Sequences Sequences were determined after subcloning amplified DNAs into pUC 18 plasmid vector (Pharmacia). Primers used for sequencing reactions are described in Wada and Satoh (1994). In order to exclude any sequence errors arising from misamplification by Taq DNA polymerase (Wada et al. ...
... template. Sequences Sequences were determined after subcloning amplified DNAs into pUC 18 plasmid vector (Pharmacia). Primers used for sequencing reactions are described in Wada and Satoh (1994). In order to exclude any sequence errors arising from misamplification by Taq DNA polymerase (Wada et al. ...
X-Chromosome dosage compensation
... for regulation by the dosage compensation complex? Fourth, how is gene expression coordinately controlled along an entire X chromosome? Fifth, what is the molecular mechanism for fine-tuning X-linked gene expression by only two-fold? These basic questions have been addressed in C. elegans, using int ...
... for regulation by the dosage compensation complex? Fourth, how is gene expression coordinately controlled along an entire X chromosome? Fifth, what is the molecular mechanism for fine-tuning X-linked gene expression by only two-fold? These basic questions have been addressed in C. elegans, using int ...
Hox Targets and Cellular Functions
... distinct cell affinities, regulate cell proliferation, and establish organ shape [40]. The identification of realizator genes was, therefore, key to understand how Hox genes shape different structures. Different approaches to isolate and characterize Hox targets in Drosophila and vertebrates were in ...
... distinct cell affinities, regulate cell proliferation, and establish organ shape [40]. The identification of realizator genes was, therefore, key to understand how Hox genes shape different structures. Different approaches to isolate and characterize Hox targets in Drosophila and vertebrates were in ...
Bacterial canker and blast of stone fruit trees, caused by
... corresponding resistance genes in the plant. In the last 2 decades, a number of pathogen avirulence genes, as well as the corresponding host resistance genes, have been cloned and identified. Resistance gene products, regardless of whether they encode resistance to viral, bacterial, fungal, nematode ...
... corresponding resistance genes in the plant. In the last 2 decades, a number of pathogen avirulence genes, as well as the corresponding host resistance genes, have been cloned and identified. Resistance gene products, regardless of whether they encode resistance to viral, bacterial, fungal, nematode ...
