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... factor (Abe et al., 2005; Wigge et al., 2005). The resulting complex activates transcription of genes that promote floral development, including AP1, thus triggering the floral transition. Activation of FT by CO is likely to be mediated through interactions with nuclear factor Y proteins (NF-Y) – also ...
Making protein (translation)
Making protein (translation)

... -The ribosome “reads” the mRNA three bases at a time. -These three bases are called the mRNA codon. ribosome ...
Engineering primary metabolic pathways of industrial
Engineering primary metabolic pathways of industrial

Identity elements in tRNA-mediated transcription
Identity elements in tRNA-mediated transcription

... T4 DNA ligase were purchased from Boehringer or New England Biolabs and used according to the instructions of the suppliers. Taq DNA polymerase and Vent DNA polymerase for use in PCR were purchased from Promega and New England Biolabs, respectively. Polynucleotide kinase was purchased from New Engla ...
Frequency of dipeptides and antidipeptides
Frequency of dipeptides and antidipeptides

... E. coli proteins). However, the C190 values of the dipeptides shown also in Table 3 are always much larger than zero. This supports the hypothesis that the trends previously described are genuine and do not depend on the fact that the amount of information is insufficient. In other words, it is poss ...
4. AMINO ACIDS
4. AMINO ACIDS

... • Most proteins contain, in varying proportions, of the same 20 L- amino acids, the kinds of amino acids, the order in which they are joined together, and their mutual spatial relationship dictate the three - dimensional structures and biologic properties of simple proteins ...
The evolutionary history of human chromosome 7
The evolutionary history of human chromosome 7

... regions at 7q22 and 7p22 in African apes, but not in the homologous chromosome regions in orangutan and gibbon. Since a detailed analysis of the WBS orthologous region on mouse chromosome 5 provided no evidence of duplicated segments, the authors concluded that these segmental duplications are of re ...
Dynamics and genetic diversification of Escherichia coli
Dynamics and genetic diversification of Escherichia coli

Reduced expression of the SHORT-ROOT gene increases the rates
Reduced expression of the SHORT-ROOT gene increases the rates

... required for proper root pattern formation, providing further evidence of a direct molecular link between these key developmental regulators and genes involved in cell-cycle progression [17]. AtSHR has therefore been shown to be important roles in regulating both cell division and cell fate determin ...
The genetic basis of inherited anomalies of the teeth: Part 1: Clinical
The genetic basis of inherited anomalies of the teeth: Part 1: Clinical

... addition to these signals, the model proposed by Thesleff [7] also includes several genes, which are regulated by the signals in the responding tissues (Fig. 1). Mutations in many of these genes already have been shown to cause dental defects in mice as well as in humans [8]. 2. Hereditary enamel de ...
Synthesis and Pharmacological Evaluation of
Synthesis and Pharmacological Evaluation of

... progress in the development of new treatments for malignant diseases, both by revealing the pathobiology of the disease and the discovery of new drugs. Moreover, the role of many proteins has been identified as novel targets in cancer therapy allowing the design of more selective agents. The classic ...
Why Sequence Alignment?
Why Sequence Alignment?

... ~2000 blocks from 500 families of related proteins • Blocks: short conserved patterns of 3-60 aa without gaps • Different BLOSUMn matrices are calculated independently from BLOCKS • BLOSUMn is based on sequences that shared at least n ...
A PCR approach to determine the distribution of toxin genes in
A PCR approach to determine the distribution of toxin genes in

Non-cell-autonomous regulation of root hair patterning genes by
Non-cell-autonomous regulation of root hair patterning genes by

... The establishment of a pattern of files forming root hairs and non-root hairs in the root epidermis is a wellstudied developmental model system (Schiefelbein et al., 2009; Tominaga-Wada et al., 2011; Grebe, 2012). Root hair cells (H-cells) develop from cell files over the cleft of two underlying cor ...
population genetics - E-Learning/An
population genetics - E-Learning/An

... In population genetics, the term polymorphism (meaning many forms) refers to the observation that many traits display variation within a population. Historically, polymorphism first referred to the variation in traits that are observable with the naked eye. Polymorphisms in color and pattern have lo ...
BI25M1
BI25M1

... [Compare with industrial (‘Haber process’) reduction of N2 to +NH4, to make agricultural fertilizer, which needs: an iron catalyst, 5000C and 300 atmospheres.] ...
The molecular basis of human retinal and vitreoretinal
The molecular basis of human retinal and vitreoretinal

... Monogenic diseases of the retina and vitreous affect approximately 1 in 2000 individuals, or more than 2 million people worldwide. Consequences for affected individuals are variable and can range from legal blindness in the most severe forms of retinal degenerations (Leber congenital amaurosis, LCA) ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
Comparison of the Structure of the Extrinsic 33 kDa Protein from

... the 33 kDa protein cDNA were amplified using primers newly synthesized based on the 469-bp cDNA fragment, which yielded a 565-bp cDNA fragment and a 640-bp cDNA fragment from 5¢- and 3¢-RACE, respectively. The nucleotide sequences of these cDNA fragments were confirmed to contain the cDNA for the 33 ...
PDF
PDF

... human adiposity have estimated that genetic factors contribute up to 40-70% of the variance of adiposity.3,4 Genome Wide Association Studies (GWAS) have contributed to researching genetic variations of multiple loci. Still, in the case of common obesity single nucleotide polymorphisms (SNPs) variati ...
Salinity Response in Chloroplasts: Insights from Gene
Salinity Response in Chloroplasts: Insights from Gene

... osmotic and ion homeostasis, abscisic acid (ABA) biosynthesis and signaling, and gene expression, as well as protein synthesis and turnover (Table 1). In this review, we summarize the genes encoding the chloroplast-localized proteins in response to salinity. 2. Salinity-Induced Diverse ROS Scavengin ...
PowerPoint Presentation - Meningitis Research Foundation
PowerPoint Presentation - Meningitis Research Foundation

... two meningococcal serogroups are commonly associated with the disease, namely B and C, representing ...
DNA and Amino Acid Comparisons
DNA and Amino Acid Comparisons

... 1. Identify
in
the
chart
below
how
many
total
differences
of
amino
acids
each
organism
had
when
 compared
to
the
human
amino
acid
sequence.
 Table B  ...
Evidence for Mitotic Crossing-over During the
Evidence for Mitotic Crossing-over During the

... have made this organism particularly attractive for studies of cell differentiation (for reviews see Bonner, 1967; Garrod & Ashworth, 1973). The application of genetic methods of analysis to this organism has, however, been delayed by the absence of any well-defined system of recombination. Previous ...
Comparison of different PCR tests for detecting Shiga toxin
Comparison of different PCR tests for detecting Shiga toxin

as a PDF
as a PDF

... control gene expression. While regulation of transcription initiation is a common regulatory strategy, it is now apparent that this is only the starting point. Bacteria have developed several sophisticated regulatory mechanisms that allow the organism to modulate gene expression after transcription ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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