Lactase persistence DNA variant enhances
... expression from a co-transfected pRL-CMV vector and expressed as relative luciferase activity (means SD, n ¼ 3). The values for p3kLac, p3kLac-C and p3kLac-T represent data from three experiments with transfections performed in triplicate, n ¼ 9. *P < 0.00001 versus p3kLac-C. ...
... expression from a co-transfected pRL-CMV vector and expressed as relative luciferase activity (means SD, n ¼ 3). The values for p3kLac, p3kLac-C and p3kLac-T represent data from three experiments with transfections performed in triplicate, n ¼ 9. *P < 0.00001 versus p3kLac-C. ...
tRNA-derived short RNAs bind to Saccharomyces cerevisiae
... tRNA-derived small RNAs can regulate protein synthesis: (i) tRNA fragments found in the phloem sap of pumpkin plants inhibited in vitro translation in wheat germ extracts (Zhang, Sun and Kragler 2009), (ii) transfection of 5 -tRNA, but not 3 -tRNA fragments triggered the assembly of stress granule ...
... tRNA-derived small RNAs can regulate protein synthesis: (i) tRNA fragments found in the phloem sap of pumpkin plants inhibited in vitro translation in wheat germ extracts (Zhang, Sun and Kragler 2009), (ii) transfection of 5 -tRNA, but not 3 -tRNA fragments triggered the assembly of stress granule ...
BIOS QTL browser - BBMRI-NL
... The BIOS consortium aims at integrating these layers of data, in order to uncover molecular pathways involved in health and disease. ...
... The BIOS consortium aims at integrating these layers of data, in order to uncover molecular pathways involved in health and disease. ...
Short Tandem Repeat Polymorphism and Cancer Risk: Influence of
... longer CAG repeats are found to be associated with lower risk of prostate cancer. However, not all epidemiologic studies have found evidence in support of this relationship. In a recent article by Giovannucci (2), 12 published studies on this subject were discussed. One third of the studies showed a ...
... longer CAG repeats are found to be associated with lower risk of prostate cancer. However, not all epidemiologic studies have found evidence in support of this relationship. In a recent article by Giovannucci (2), 12 published studies on this subject were discussed. One third of the studies showed a ...
Pitx1 and Pitx2 are required for development of hindlimb buds
... explain the poor viability of these mice. This was not observed with the Pitx2neo allele, which gave close to the expected yield (20%) when crossed with Pitx1+/– mice. Double mutant embryos (Pitx1–/–,Pitx2+/–) were obtained by crossing Pitx1+/– mice with Pitx1+/–,Pitx2+/– mice. We only ever got one ...
... explain the poor viability of these mice. This was not observed with the Pitx2neo allele, which gave close to the expected yield (20%) when crossed with Pitx1+/– mice. Double mutant embryos (Pitx1–/–,Pitx2+/–) were obtained by crossing Pitx1+/– mice with Pitx1+/–,Pitx2+/– mice. We only ever got one ...
Temperature-Sensitive Mutations Made Easy: Generating
... S16 and S22 enabled their host yeast to grow at 30°, and thus were discarded. Forty-two alleles were eliminated at this step because of leaky splicing at 30°. Step 5: To eliminate duplicate TS alleles. The remaining 49 alleles were sequenced, and 40 unique alleles were identified (Figure S2). No obv ...
... S16 and S22 enabled their host yeast to grow at 30°, and thus were discarded. Forty-two alleles were eliminated at this step because of leaky splicing at 30°. Step 5: To eliminate duplicate TS alleles. The remaining 49 alleles were sequenced, and 40 unique alleles were identified (Figure S2). No obv ...
nitrogen fixation and its improvement through genetic engineering
... sp.) with the soil bacteria of genus Frankia, an actinomycete (Wagner, 2012). Nitrogen fixation is an “old” topic in scientific terms since it is over a century ago that scientists experimentally proved that some “unique” species of plants with the help of microbes that were later found bearing nitr ...
... sp.) with the soil bacteria of genus Frankia, an actinomycete (Wagner, 2012). Nitrogen fixation is an “old” topic in scientific terms since it is over a century ago that scientists experimentally proved that some “unique” species of plants with the help of microbes that were later found bearing nitr ...
Unbalanced translocation, a major chromosome alteration
... cancers also suffered chromosomal alterations associated with copy number changes but not with LOH. Consistent with the result of array-CGH analysis, all the seven cell lines were defined as being nondiploid with multiple structural chromosome alterations by SKY analysis. Classification of LOHs by chr ...
... cancers also suffered chromosomal alterations associated with copy number changes but not with LOH. Consistent with the result of array-CGH analysis, all the seven cell lines were defined as being nondiploid with multiple structural chromosome alterations by SKY analysis. Classification of LOHs by chr ...
Puffs and PCR: the in vivo dynamics of early gene
... to 55°C. 1 µl of a mix of AMV RTase (2.5 units, Pharmacia) and Taq polymerase (1.25 units, Cetus) was added and the tube left for 20 minutes at 55°C. The subsequent amplification programme was 3 minutes at 94°C followed by 30 cycles of 94°C (1 minute), 55°C (2 minutes) and 72°C (3 minutes) followed ...
... to 55°C. 1 µl of a mix of AMV RTase (2.5 units, Pharmacia) and Taq polymerase (1.25 units, Cetus) was added and the tube left for 20 minutes at 55°C. The subsequent amplification programme was 3 minutes at 94°C followed by 30 cycles of 94°C (1 minute), 55°C (2 minutes) and 72°C (3 minutes) followed ...
Limb Girdle Muscular Dystrophy - Muscular Dystrophy Foundation of
... Autosomal recessive muscular dystrophy of childhood: This condition may be confused with Duchenne Muscular Dystrophy (DMD) in boys, and in girls may be difficult to distinguish from the manifesting carrier state, though the mode of inheritance is completely different. Either sex may be affected, and ...
... Autosomal recessive muscular dystrophy of childhood: This condition may be confused with Duchenne Muscular Dystrophy (DMD) in boys, and in girls may be difficult to distinguish from the manifesting carrier state, though the mode of inheritance is completely different. Either sex may be affected, and ...
Mapping Post-Transcriptional Modifications onto Transfer
... RNA. Not only are tRNAs heavily decorated, the types of chemical covalent modifications are rich and varied in both structure and biosynthesis routes. To date, more than 100 different chemical modifications have been characterized on tRNAs from all three domains of life, and two data ...
... RNA. Not only are tRNAs heavily decorated, the types of chemical covalent modifications are rich and varied in both structure and biosynthesis routes. To date, more than 100 different chemical modifications have been characterized on tRNAs from all three domains of life, and two data ...
LEADING ARTICLE Concurrent disruption of p16INK4a and
... cycle progression by inhibiting the activity of a broader range of CDKs.26–28 Genetic and epigenetic alterations of p16INK4a and p53 have been found in several subtypes of NHL, in particular in the progressed variants, yet little is known about their prognostic significance.4–22 A single study has d ...
... cycle progression by inhibiting the activity of a broader range of CDKs.26–28 Genetic and epigenetic alterations of p16INK4a and p53 have been found in several subtypes of NHL, in particular in the progressed variants, yet little is known about their prognostic significance.4–22 A single study has d ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
... More and more human SNPs are analyzed for their potential association with diseases, risk factors and predispositions. Our LightSNiP assays are preestablished, probe-based tests using a melting curve to detect sequence variations. These assays are developed on the Roche LightCycler® 480 system, but ...
... More and more human SNPs are analyzed for their potential association with diseases, risk factors and predispositions. Our LightSNiP assays are preestablished, probe-based tests using a melting curve to detect sequence variations. These assays are developed on the Roche LightCycler® 480 system, but ...
Document
... Where can phylogeny be used • For example, finding out about orthology versus paralogy • Predicting secondary structure of RNA • Predicting protein-protein interaction • Studying host-parasite relationships • Mapping cell-bound receptors onto their binding ligands • Multiple sequence alignment (e.g ...
... Where can phylogeny be used • For example, finding out about orthology versus paralogy • Predicting secondary structure of RNA • Predicting protein-protein interaction • Studying host-parasite relationships • Mapping cell-bound receptors onto their binding ligands • Multiple sequence alignment (e.g ...
Lecture4 - Department of Computer Science
... BLAST for a match to be significant. • Speed and sensitivity can be improved by requiring the algorithm find two matches above some (lower) threshold rather that one match. Both matches must be on the same diagonal. ...
... BLAST for a match to be significant. • Speed and sensitivity can be improved by requiring the algorithm find two matches above some (lower) threshold rather that one match. Both matches must be on the same diagonal. ...
Inhibition of Wound-Induced Accumulation of
... epoxides, which supply the precursors for the synthesis of the phytohormone jasmonic acid (JA). In this study the characterization of AOS gene expression in flax (Linum usitatissimum) is reported. AOS was constitutively expressed in different organs of flax plants. Additionally, AOS gene expression ...
... epoxides, which supply the precursors for the synthesis of the phytohormone jasmonic acid (JA). In this study the characterization of AOS gene expression in flax (Linum usitatissimum) is reported. AOS was constitutively expressed in different organs of flax plants. Additionally, AOS gene expression ...
TCF11/Nrf1 overexpression increases the intracellular
... 35 mm culture well. Thirty-six to 40 h post transfection the luciferase activity in cell lysates was measured. A 2.7fold increase in luciferase activity was observed at the highest concentration of the TCF11/Nrf1 expression plasmid used (Fig. 2). The same results were obtained when cells were transf ...
... 35 mm culture well. Thirty-six to 40 h post transfection the luciferase activity in cell lysates was measured. A 2.7fold increase in luciferase activity was observed at the highest concentration of the TCF11/Nrf1 expression plasmid used (Fig. 2). The same results were obtained when cells were transf ...
Structure-Function Analysis of the Conserved Histone Chaperone
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
... Chromatin structure is crucial to regulate access to the genome for processes such as transcription, recombination, DNA repair, and DNA replication. Spt6, a key factor involved in regulating chromatin struct ...
Identity elements in tRNA-mediated transcription
... T4 DNA ligase were purchased from Boehringer or New England Biolabs and used according to the instructions of the suppliers. Taq DNA polymerase and Vent DNA polymerase for use in PCR were purchased from Promega and New England Biolabs, respectively. Polynucleotide kinase was purchased from New Engla ...
... T4 DNA ligase were purchased from Boehringer or New England Biolabs and used according to the instructions of the suppliers. Taq DNA polymerase and Vent DNA polymerase for use in PCR were purchased from Promega and New England Biolabs, respectively. Polynucleotide kinase was purchased from New Engla ...
Identity elements in tRNA-mediated transcription
... T4 DNA ligase were purchased from Boehringer or New England Biolabs and used according to the instructions of the suppliers. Taq DNA polymerase and Vent DNA polymerase for use in PCR were purchased from Promega and New England Biolabs, respectively. Polynucleotide kinase was purchased from New Engla ...
... T4 DNA ligase were purchased from Boehringer or New England Biolabs and used according to the instructions of the suppliers. Taq DNA polymerase and Vent DNA polymerase for use in PCR were purchased from Promega and New England Biolabs, respectively. Polynucleotide kinase was purchased from New Engla ...