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... a. The general transcription factors (GTFs) are proteins required for accurate and efficient transcription that are not subunits of purified RNA polymerase. We will focus primarily on the general transcription initiation factors (GTIFs), which are proteins needed for accurate initiation of transcrip ...
Rapid and accurate cancer somatic mutation
Rapid and accurate cancer somatic mutation

... Mutation PCR Arrays that simultaneously and accurately detect 80 to 370 somatic mutations in the same sample. The pathways covered include major receptor tyrosine kinase pathways and non-receptor kinase pathways, as well as additional oncogene and tumor suppressor pathways. The targeted diseases inc ...
View/Open
View/Open

... major criteria independent to the presence of minor criteria; Group 2, incomplete FMF exhibiting the absence of major criteria and 2 or more minor criteria. It is important to stress that response to colchicine was confirmed in almost all patients. As controls, 200 healthy Japanese individuals witho ...
Participation of DDDD and KPAR
Participation of DDDD and KPAR

... Mercuric reductase (MerA) is an essential enzyme for the survival of microorganisms that reside in environments containing mercuric compounds. The enzyme converts the extremely toxic mercuric ions (Hg2+) into the less toxic volatile elemental mercury form (Hg 0). A novel MerA molecule that has under ...
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... site of Minos in the EJ[MiTSAdTPOG]124 transgenic line. Exons are indicated by boxes. Gray and white boxes correspond to the open reading frame and untranslated regions, respectively. (B,C)A wild-type heterozygous Ciona intestinalis juvenile (left) and an ngs mutant (right). Scale bar: 100m. (D) ...
BIOGENESIS OF MITOCHONDRIAL ATPase Studies on
BIOGENESIS OF MITOCHONDRIAL ATPase Studies on

... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
Ribosomal proteins promote leaf adaxial identity
Ribosomal proteins promote leaf adaxial identity

... panel) and 3 (B, upper panel), and AE5 (At2g19730) and AE6 (At3g25520) encode ribosomal proteins RPL28A (A, lower panel) and RPL5A (B, lower panel), respectively. Black and white boxes indicate the protein-coding region and untranslational region (UTR), respectively. (C) RT-PCR shows that AE5 and AE ...
Ch 6 LIPID METABOLISM - FORMATTED - NSDL
Ch 6 LIPID METABOLISM - FORMATTED - NSDL

... Oxidation of fatty acids can occur at α, β or ω carbon atoms of their ‘R’ chains (refer chapter 2 section 2). We will deal briefly with the two less common modes of fatty acid oxidation viz. α- and ω-oxidations, before taking up the most prevalent mode of oxidation i.e. β-oxidation, in more detail. ...
Evolutionary dynamics of nematode operons
Evolutionary dynamics of nematode operons

... genes are present, some of them have become pseudogenes. For example, after the operon breakage, the downstream gene in a two-gene operon may be pseudogenized, equivalent to gene loss. Using GeneWise (Birney et al. 2004), we examined whether the open reading frame (ORF) was still intact for each dow ...
Global Transcriptional Profiling Reveals Distinct Functions of Thymic
Global Transcriptional Profiling Reveals Distinct Functions of Thymic

Visualization, description and analysis of the Drosophila melanogaster
Visualization, description and analysis of the Drosophila melanogaster

SMAD proteins of oligodendroglial cells regulate transcription of JC
SMAD proteins of oligodendroglial cells regulate transcription of JC

... promoter was ,2 RLU, whilst that of the E promoter was approximately 10 RLU. Differences in the exact basal RLU numbers between Figs 1 and 2 were probably due to minor differences in the numbers of cells cultured. In this case, stimulation of the E promoter was more than 7-fold at the optimum concen ...
a review on biochemical mechanism of fatty acids synthesis and oil
a review on biochemical mechanism of fatty acids synthesis and oil

... In plants, fatty acid biosynthesis process initiated in plastid and ended in the endoplasmic reticulum. A series of biochemical steps are involved in TAG formation and many of the genes involved in this process have been identified. The quality of seed oil depends on the type of fatty acids esterifi ...
Azole antifungals are potent inhibitors of cytochrome
Azole antifungals are potent inhibitors of cytochrome

... people will become infected with Mtb, 200 million will become sick because of it and around 35 million will die from tuberculosis. At present, approximately one-third of the world’s population is infected with tuberculosis, and the worldwide rate of new infections runs at one individual per second ( ...
An Introduction to Genetic Analysis Chapter 18 Chromosome
An Introduction to Genetic Analysis Chapter 18 Chromosome

... be induced by cold treatment to grow into an embryoid, a small dividing mass of cells. The embryoid can be grown on agar to form a monoploid plantlet, which can then be potted in soil and allowed to mature (Figure 18-1). Plant monoploids can be exploited in several ways. In one, they are first exami ...
Engineering Melon Plants with Improved Fruit Shelf Life
Engineering Melon Plants with Improved Fruit Shelf Life

... than 40 seeds per fruit (Table 1). Thus, the highest EMS doses allowing good seed set and seed germination, 1.5% and 2%, were retained and tested on large batches of seeds. The EMS treated seeds were sown in soil and seedlings were grown to fruit maturity in insect-proof plastic tunnels to avoid cro ...
SAMHD1 is a single-stranded nucleic acid
SAMHD1 is a single-stranded nucleic acid

... oligonucleotides were obtained from Integrated DNA Technologies (IDT). The 90 nt RNA oligonucleotide was produced via in vitro transcription with T7 RNA Polymerase and a polymerase chain reaction (PCR)-amplified runoff transcript. The RNA was isolated by Qiagen RNeasy Mini Kit. The 5 -FAM labeled 1 ...
Microbiology 146:
Microbiology 146:

... characterization of a 72 kb fragment of R. etli DNA harbouring the structural genes gltB and gltD encoding the two GOGAT subunits GltB and GltD is also reported. In comparison with the wild-type strain (CFN42), the GOGAT mutant strain utilized less succinate and glutamate and grew less with this an ...
Diapositiva 1 - Universidad Autónoma de San Luis Potosí
Diapositiva 1 - Universidad Autónoma de San Luis Potosí

... The three stages Initiation involves the reactions that precede formation of the peptide bond between the first two amino acids of the protein. It requires the ribosome to bind to the mRNA, forming an initiation complex that contains the first aminoacyl-tRNA. This is a relatively slow step in prote ...
Chromosome numbers in female and male gametes: One
Chromosome numbers in female and male gametes: One

... account for some of the pollen abortion observed in el plants, Hypotheses on the action of the el gene: It is evident from the above presentation that el plants produce both haploid eggs with ten chromosomes and unreduced eggs at the diploid level although some have less than 20 chromosomes and some ...
Transcription Factor EB Controls Metabolic Flexibility during
Transcription Factor EB Controls Metabolic Flexibility during

... mitochondrial biogenesis observed in TFEB-transfected muscles, we examined the expression of nuclear respiratory factors 1 and 2 (NRF1and NRF2). The mRNA levels of NRF2 were increased, as well as NRF downstream genes, including mitochondrial transcription factor A (TFAM). Chromatin immunoprecipitat ...
Genome-wide association studies for complex traits: consensus
Genome-wide association studies for complex traits: consensus

Unified display of Arabidopsis thaliana physical maps from AtDB, the
Unified display of Arabidopsis thaliana physical maps from AtDB, the

... Display of the physical-map data The unified display of the physical maps is clickable as are other AtDB displays (5). By this method, new mapping information can be incorporated into the display simply by updating the database. The picture presented to the user will then be altered as soon as new d ...
BIOS QTL browser - BBMRI-NL
BIOS QTL browser - BBMRI-NL

... The BIOS consortium aims at integrating these layers of data, in order to uncover molecular pathways involved in health and disease. ...
tRNA-derived short RNAs bind to Saccharomyces cerevisiae
tRNA-derived short RNAs bind to Saccharomyces cerevisiae

... tRNA-derived small RNAs can regulate protein synthesis: (i) tRNA fragments found in the phloem sap of pumpkin plants inhibited in vitro translation in wheat germ extracts (Zhang, Sun and Kragler 2009), (ii) transfection of 5 -tRNA, but not 3 -tRNA fragments triggered the assembly of stress granule ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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