The connection of cytoskeletal network with plasma membrane and
... 2013). Therefore, it is possible that CLASP could form a transient association between the microtubules and the plasma membrane via retromer associated vesicles. Importantly, only short stretches of the microtubules displayed detachment from the plasma membrane in clasp-1, and the detached microtubu ...
... 2013). Therefore, it is possible that CLASP could form a transient association between the microtubules and the plasma membrane via retromer associated vesicles. Importantly, only short stretches of the microtubules displayed detachment from the plasma membrane in clasp-1, and the detached microtubu ...
Poster
... β-catenin is a multi-functional protein involved in two essential cellular pathways: cell-cell adhesion and transcriptional regulation8. β-catenin contains 12 armadillo repeats capped by a C-helix. An amino acid important for β-catenin’s electrostatic interactions with ligands is Lys-435 known as th ...
... β-catenin is a multi-functional protein involved in two essential cellular pathways: cell-cell adhesion and transcriptional regulation8. β-catenin contains 12 armadillo repeats capped by a C-helix. An amino acid important for β-catenin’s electrostatic interactions with ligands is Lys-435 known as th ...
G1 Phase-Dependent Expression of Bcl
... Recent experiments suggest an interconnection between cell proliferation and programmed cell death (apoptosis), although the detailed molecular mechanisms remain unclear. We have hypothesized that expression of some apoptosis regulators is cell cycle-dependent, which in turn influences tumor cell ch ...
... Recent experiments suggest an interconnection between cell proliferation and programmed cell death (apoptosis), although the detailed molecular mechanisms remain unclear. We have hypothesized that expression of some apoptosis regulators is cell cycle-dependent, which in turn influences tumor cell ch ...
Models of infectious diseases in the fruit fly Drosophila melanogaster
... this process because S. pneumoniae doesn’t induce transcriptional changes that are typically dependent on Imd. However, this overlooks important information. The patient would benefit if it learned that the loss of Imd would make them more susceptible to S. pneumoniae, even though the exact mechanis ...
... this process because S. pneumoniae doesn’t induce transcriptional changes that are typically dependent on Imd. However, this overlooks important information. The patient would benefit if it learned that the loss of Imd would make them more susceptible to S. pneumoniae, even though the exact mechanis ...
Hedgehog and Dpp signaling induce cadherin Cad86C expression
... Ectopic expression of CiPKA4, an activated form of the Znfinger containing protein Cubitus interruptus (Ci) (Methot and Basler, 2000), in clones of cells located anterior to the morphogenetic furrow resulted in apical cell constriction ...
... Ectopic expression of CiPKA4, an activated form of the Znfinger containing protein Cubitus interruptus (Ci) (Methot and Basler, 2000), in clones of cells located anterior to the morphogenetic furrow resulted in apical cell constriction ...
Pascale G. Charest and Michel Bouvier Activation
... of -arrestin proteins preferentially binding to receptors phosphorylated by GPCR kinases. In addition to promoting receptor/G protein uncoupling, the -arrestins target such desensitized receptors to clathrin-coated pits for endocytosis by functioning as adaptor proteins that link the receptors to ...
... of -arrestin proteins preferentially binding to receptors phosphorylated by GPCR kinases. In addition to promoting receptor/G protein uncoupling, the -arrestins target such desensitized receptors to clathrin-coated pits for endocytosis by functioning as adaptor proteins that link the receptors to ...
PDF
... Kewley, 2004). Members of the bHLH-PAS protein family are characterized by an N-terminal bHLH domain required for DNA binding and dimerization, as well as by a PAS domain that acts as a secondary dimerization interface (Crews and Fan, 1999; Taylor and Zhulin, 1999). Transcriptional control of target ...
... Kewley, 2004). Members of the bHLH-PAS protein family are characterized by an N-terminal bHLH domain required for DNA binding and dimerization, as well as by a PAS domain that acts as a secondary dimerization interface (Crews and Fan, 1999; Taylor and Zhulin, 1999). Transcriptional control of target ...
Full Text
... in lysosomes of cells, giving rise to the lysosomal storage diseases (Cervos-Navarro and Urich, 1995). Later on, the discovery of oligosaccharides in the urine of patients with lysosomal storage diseases (Wolfe et al., 1974), and their structural study (Nishigaki et al., 1978; Yamashita et al., 1979 ...
... in lysosomes of cells, giving rise to the lysosomal storage diseases (Cervos-Navarro and Urich, 1995). Later on, the discovery of oligosaccharides in the urine of patients with lysosomal storage diseases (Wolfe et al., 1974), and their structural study (Nishigaki et al., 1978; Yamashita et al., 1979 ...
PDF - Bezanilla Lab
... a stable moss line where the For2A locus was modified by homologous recombination. Sequences encoding for 3 tandem GFPs were integrated at the 3⬘ end of For2A. Fluorescence from For2A3XmEGFP was found concentrated at the very apex of growing cells (Fig. 4). This localization reflects that of a funct ...
... a stable moss line where the For2A locus was modified by homologous recombination. Sequences encoding for 3 tandem GFPs were integrated at the 3⬘ end of For2A. Fluorescence from For2A3XmEGFP was found concentrated at the very apex of growing cells (Fig. 4). This localization reflects that of a funct ...
Fission yeast Tor2 promotes cell growth and represses cell
... proteins. As shown in Fig. 2B, Tor1 coimmunoprecipitated with Ste20 but not with Mip1, and Ste20 co-immunoprecipitated with Tor1 but not with Tor2. Furthermore, Tor2 coimmunoprecipitated with Mip1 but not with Ste20, and Mip1 co-immunoprecipitated with Tor2 but not with Tor1. However, Pop3 coimmunop ...
... proteins. As shown in Fig. 2B, Tor1 coimmunoprecipitated with Ste20 but not with Mip1, and Ste20 co-immunoprecipitated with Tor1 but not with Tor2. Furthermore, Tor2 coimmunoprecipitated with Mip1 but not with Ste20, and Mip1 co-immunoprecipitated with Tor2 but not with Tor1. However, Pop3 coimmunop ...
Transcriptional mechanisms regulating skeletal muscle
... and/or enable a default differentiation pathway (permissive induction) or primarily prevent programmed cell death of muscle progenitor cells (reviewed in REF. 139). From numerous studies, it is clear that sonic hedgehog (SHH) and WNT signalling have pivotal roles in the induction of myogenesis. Mor ...
... and/or enable a default differentiation pathway (permissive induction) or primarily prevent programmed cell death of muscle progenitor cells (reviewed in REF. 139). From numerous studies, it is clear that sonic hedgehog (SHH) and WNT signalling have pivotal roles in the induction of myogenesis. Mor ...
Plant Cell
... et al., 2014). In support of this hypothesis is a recent study demonstrating that the calcium dependent protein kinase 5 (CPK5) is important for the propagation of the ROS wave in plants (Romeis and Herde, 2014). ROS generated in the apoplast by an RBOHD protein of a neighboring cell (as part of the ...
... et al., 2014). In support of this hypothesis is a recent study demonstrating that the calcium dependent protein kinase 5 (CPK5) is important for the propagation of the ROS wave in plants (Romeis and Herde, 2014). ROS generated in the apoplast by an RBOHD protein of a neighboring cell (as part of the ...
Interleukin-4 Deficiency Decreases Atherosclerotic Lesion
... IL-4⫺/⫺ or IL-4⫹/⫹ mice were transplanted into lethally irradiated female low density lipoprotein (LDL) receptor⫺/⫺ mice. After an interval sufficient to allow engraftment, mice were placed on a diet containing 21% saturated fat, 1.25% cholesterol, and 0.5% cholate. Hematopoietic engraftment was con ...
... IL-4⫺/⫺ or IL-4⫹/⫹ mice were transplanted into lethally irradiated female low density lipoprotein (LDL) receptor⫺/⫺ mice. After an interval sufficient to allow engraftment, mice were placed on a diet containing 21% saturated fat, 1.25% cholesterol, and 0.5% cholate. Hematopoietic engraftment was con ...
Nucleolar localization of an isoform of the IGF
... residues VKM [-27 to -25] to A [-1]. This was cloned upstream of the cDNA for GFP. Exon 3 encodes the mature IGF-I peptide starting at residues GPET [1–4] to F [25], exon 4 encodes the mature IGF-I peptide from residue N [26] to A [70] and part of the E domain from residues RSV [71–73] to K [86]. Ex ...
... residues VKM [-27 to -25] to A [-1]. This was cloned upstream of the cDNA for GFP. Exon 3 encodes the mature IGF-I peptide starting at residues GPET [1–4] to F [25], exon 4 encodes the mature IGF-I peptide from residue N [26] to A [70] and part of the E domain from residues RSV [71–73] to K [86]. Ex ...
plantcell.org
... were digested with AvaII (Figure 3B). Two W22-specific fragments (;4.0 and 8.5 kb) and two A69Y-specific fragments (;2.7 and 4.5 kb) were present in endosperm samples only when W22 and A69Y lines were used as a pollen source, respectively, but not when used as females, indicating that meg1 maternal ...
... were digested with AvaII (Figure 3B). Two W22-specific fragments (;4.0 and 8.5 kb) and two A69Y-specific fragments (;2.7 and 4.5 kb) were present in endosperm samples only when W22 and A69Y lines were used as a pollen source, respectively, but not when used as females, indicating that meg1 maternal ...
Stimulation of Cell Elongation by Tetraploidy in Hypocotyls of Dark
... autopolyploidy and endopolyploidy, both of which have been correlated with cytoplasmic volume and, hence, cell growth [1]. Endopolyploidy, which is essential for angiosperm development, is controlled by an endoreduplication cycle whereby the genome is duplicated without cellular division. Conversely ...
... autopolyploidy and endopolyploidy, both of which have been correlated with cytoplasmic volume and, hence, cell growth [1]. Endopolyploidy, which is essential for angiosperm development, is controlled by an endoreduplication cycle whereby the genome is duplicated without cellular division. Conversely ...
An Isotype-specific Activator of Major Histocompatibility Complex
... (EMSA)1 followed by protein purification or, more recently, by complementation/expression cloning. The latter process has taken advantage of the existence of both MHC class II CID patient- and experimentally derived mutant cell lines that do not express class II genes but can be induced to do so up ...
... (EMSA)1 followed by protein purification or, more recently, by complementation/expression cloning. The latter process has taken advantage of the existence of both MHC class II CID patient- and experimentally derived mutant cell lines that do not express class II genes but can be induced to do so up ...
Author`s personal copy
... at the 16-cell stage, 8 vegetal blastomeres divide before the 8 animal blastomeres creating a brief 24-cell stage embryo. Remarkably, this pattern of cell division has been conserved in very distantly-related ascidian species from different orders (e.g. Halocynthia roretzi and Phallusia mammillata). ...
... at the 16-cell stage, 8 vegetal blastomeres divide before the 8 animal blastomeres creating a brief 24-cell stage embryo. Remarkably, this pattern of cell division has been conserved in very distantly-related ascidian species from different orders (e.g. Halocynthia roretzi and Phallusia mammillata). ...
The ventral and posterior expression of the zebrafish homeobox
... vertebrate models, cellular characteristics and embryological origins of caudal structures are variable (May Griffith et al., 1992). In bird and mammals, the tail bud results from the coalescence between Hensen’s node and the primitive streak at the end of gastrulation. In amphibians, there is no ca ...
... vertebrate models, cellular characteristics and embryological origins of caudal structures are variable (May Griffith et al., 1992). In bird and mammals, the tail bud results from the coalescence between Hensen’s node and the primitive streak at the end of gastrulation. In amphibians, there is no ca ...
Plant Wnt: deciphering a novel signalling pathway
... cascade, the non-canonical pathway does not bank on catenin to carry out downstream signalling, hence is known as -catenin independent Wnt signalling. PCP signalling is involved mainly in the regulation of cell polarity during morphogenesis by activating JNK-dependent transcription factors via sma ...
... cascade, the non-canonical pathway does not bank on catenin to carry out downstream signalling, hence is known as -catenin independent Wnt signalling. PCP signalling is involved mainly in the regulation of cell polarity during morphogenesis by activating JNK-dependent transcription factors via sma ...
Resistance Gene-Dependent Activation of a Calcium
... steps. AOS production, MAP kinase activation, defense gene activation, and phytoalexin production, singly or in combination, were shown to be compromised in the presence of Ca2⫹-chelating or Ca2⫹ channel–inhibiting compounds (Scheel, 1998). In addition, an increase in cytosolic Ca2⫹ was detected aft ...
... steps. AOS production, MAP kinase activation, defense gene activation, and phytoalexin production, singly or in combination, were shown to be compromised in the presence of Ca2⫹-chelating or Ca2⫹ channel–inhibiting compounds (Scheel, 1998). In addition, an increase in cytosolic Ca2⫹ was detected aft ...