Somatic Cytokinesis and Pollen Maturation in Arabidopsis
... were also observed in the representative bright-field images (Figures 2C and 2D). The deposits occurred mostly at the cell periphery; therefore, the intine cell wall composition was analyzed using calcofluor white and aniline blue staining methods. A mixture of aniline blue and calcofluor white dist ...
... were also observed in the representative bright-field images (Figures 2C and 2D). The deposits occurred mostly at the cell periphery; therefore, the intine cell wall composition was analyzed using calcofluor white and aniline blue staining methods. A mixture of aniline blue and calcofluor white dist ...
Developmental genetics of the Caenorhabditis elegans pharynx
... defects. Mutant screen: Avery.18 Describes the first and probably most successful screen for mutants with pharyngeal defects. The methodology is powerful and many mutants are even finely mapped, though some remain to be molecularly defined even to this day. Electropharyngeograms: Avery et al.19 Present ...
... defects. Mutant screen: Avery.18 Describes the first and probably most successful screen for mutants with pharyngeal defects. The methodology is powerful and many mutants are even finely mapped, though some remain to be molecularly defined even to this day. Electropharyngeograms: Avery et al.19 Present ...
Cell shape changes indicate a role for extrinsic tensile forces in
... this axis in wild-type embryos (Fig. 2e). In kni hb embryos, which lack AP patterning in the trunk, cell intercalation was abolished throughout the field of view, as expected (Supplementary Information, Fig. 3g). In Krüppel− embryos, cell intercalation was abolished in a central region, and decrease ...
... this axis in wild-type embryos (Fig. 2e). In kni hb embryos, which lack AP patterning in the trunk, cell intercalation was abolished throughout the field of view, as expected (Supplementary Information, Fig. 3g). In Krüppel− embryos, cell intercalation was abolished in a central region, and decrease ...
A Notch feeling of somite segmentation and beyond
... found as a broad domain in the posterior PSM and tail bud. As these cells mature anteriorly, the cycles of gene expression are slowed down and finally arrested, and c-hairy1 expression becomes limited to the posterior half of the formed somite (see Fig. 2 for a similar pattern of deltaC). Importantl ...
... found as a broad domain in the posterior PSM and tail bud. As these cells mature anteriorly, the cycles of gene expression are slowed down and finally arrested, and c-hairy1 expression becomes limited to the posterior half of the formed somite (see Fig. 2 for a similar pattern of deltaC). Importantl ...
Functional interaction between a novel protein phosphatase 2A
... The notion that PP2A plays a role in cell cycle regulation is supported by several observations. First, okadaic acid, a strong inhibitor of PP2A, is a tumor promoting agent (Mumby and Walter, 1993). Furthermore, PP2A is a target of transforming proteins of several DNA tumor viruses (Sontag et al., 1 ...
... The notion that PP2A plays a role in cell cycle regulation is supported by several observations. First, okadaic acid, a strong inhibitor of PP2A, is a tumor promoting agent (Mumby and Walter, 1993). Furthermore, PP2A is a target of transforming proteins of several DNA tumor viruses (Sontag et al., 1 ...
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... To investigate this hypothesis further, we analysed SWH pathway activity in eye tissue lacking core or non-core SWH pathway genes by measuring the expression of the wellcharacterised SWH pathway target genes DIAP1, ex and ban. Expression levels were determined at both the third instar larval and mid ...
... To investigate this hypothesis further, we analysed SWH pathway activity in eye tissue lacking core or non-core SWH pathway genes by measuring the expression of the wellcharacterised SWH pathway target genes DIAP1, ex and ban. Expression levels were determined at both the third instar larval and mid ...
THE ROLE OF 5-ALPHA REDUCTASE 3 IN STEROID METABOLISM
... Despite the discovery of a surprising role of SRD5A3 in protein glycosylation, this enzyme’s localization and role in prostate cancer remained a mystery. Two studies by Godoy and Yamana analyzed the distribution of SRD5A3 in human tissues using immunohistochemical studies (49, 50). They found that S ...
... Despite the discovery of a surprising role of SRD5A3 in protein glycosylation, this enzyme’s localization and role in prostate cancer remained a mystery. Two studies by Godoy and Yamana analyzed the distribution of SRD5A3 in human tissues using immunohistochemical studies (49, 50). They found that S ...
Embryonic Stem Cell Culture Conditions Support Distinct States
... the blastocyst from which they are derived. However, does this heterogeneity reflect the endogenous cell populations that arise in normal blastocyst development? The functional potential of ESCs can be assessed using a number of different approaches including in vitro differentiation, teratoma forma ...
... the blastocyst from which they are derived. However, does this heterogeneity reflect the endogenous cell populations that arise in normal blastocyst development? The functional potential of ESCs can be assessed using a number of different approaches including in vitro differentiation, teratoma forma ...
Fluorescence resonance energy transfer (FRET) microscopy
... membranes and dissociating it from Rho-GDI (guanine nucleotide dissociation inhibitors), and also that in spite of its homogeneous distribution in the cell, constitutively active Rac selectively interacts with effectors at specific regions of the cell edge (Pozo et al., 2002) (Fig. 1). The onset and ...
... membranes and dissociating it from Rho-GDI (guanine nucleotide dissociation inhibitors), and also that in spite of its homogeneous distribution in the cell, constitutively active Rac selectively interacts with effectors at specific regions of the cell edge (Pozo et al., 2002) (Fig. 1). The onset and ...
Integrin cytoplasmic domain-binding proteins
... colocalizes with αIIbβ3 expressed in CHO cells, and microinjection of the integrin-binding domain of skelemin causes myoblasts to round up (Reddy et al., 1998), conceivably by disrupting integrin-cytoskeleton interactions. Skelemin is encoded by an alternatively spliced version of the myomesin gene ...
... colocalizes with αIIbβ3 expressed in CHO cells, and microinjection of the integrin-binding domain of skelemin causes myoblasts to round up (Reddy et al., 1998), conceivably by disrupting integrin-cytoskeleton interactions. Skelemin is encoded by an alternatively spliced version of the myomesin gene ...
Arabidopsis RHD3 mediates the generation of the tubular ER
... RHD3 in the generation of the tubular ER network complements the Arabidopsis rhd3-1 mutant throughout the development of root, root hairs and adult plants (Fig. 1A–D; see also Fig. 3A and Fig. 8A for rhd3-1 phenotype). Confocal imaging of rhd3-1::GFP–RHD3 root epidermal cells revealed that the fusio ...
... RHD3 in the generation of the tubular ER network complements the Arabidopsis rhd3-1 mutant throughout the development of root, root hairs and adult plants (Fig. 1A–D; see also Fig. 3A and Fig. 8A for rhd3-1 phenotype). Confocal imaging of rhd3-1::GFP–RHD3 root epidermal cells revealed that the fusio ...
Serotonin synchronises convergent extension of
... appears which are 5-ht2Dro-speci®c since they are pharmacologically indistinguishable from those of 5-ht2Dro transfected cells (Colas et al., 1995). As previously reported, concomitant with this expression, there is also a transient peak of 5-HT synthesis (Colas et al., 1995). The serotonin receptor ...
... appears which are 5-ht2Dro-speci®c since they are pharmacologically indistinguishable from those of 5-ht2Dro transfected cells (Colas et al., 1995). As previously reported, concomitant with this expression, there is also a transient peak of 5-HT synthesis (Colas et al., 1995). The serotonin receptor ...
Lymphatic endothelial differentiation: start out with Sox
... Clearly, Sox18 is part of an essential decision process upstream of Prox1. However, in contrast to Prox1, Sox18 is neither indispensable nor likely to act single-handedly during lymphatic differentiation, as is indicated by the normal lymphatic development in Sox18-knockout mice on an outbreed backg ...
... Clearly, Sox18 is part of an essential decision process upstream of Prox1. However, in contrast to Prox1, Sox18 is neither indispensable nor likely to act single-handedly during lymphatic differentiation, as is indicated by the normal lymphatic development in Sox18-knockout mice on an outbreed backg ...
during T Lymphocyte Activation Coordinately Regulated by ERK
... http://www.jimmunol.org/content/185/2/1037.full#ref-list-1 Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Submit copyright permission requests at: ...
... http://www.jimmunol.org/content/185/2/1037.full#ref-list-1 Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Submit copyright permission requests at: ...
PDF + SI - Development - The Company of Biologists
... Development Advance Online Articles. First posted online on 10 June 2016 as 10.1242/dev.137224 Access the most recent version at http://dev.biologists.org/lookup/doi/10.1242/dev.137224 ...
... Development Advance Online Articles. First posted online on 10 June 2016 as 10.1242/dev.137224 Access the most recent version at http://dev.biologists.org/lookup/doi/10.1242/dev.137224 ...
T cell metabolism drives immunity
... group identified mice with enhanced CD8 T cell responses to viral and tumor challenge (Okoye et al., 2015). The source of the heightened immunity gained after germline mutagenesis was the increased expression of an orphan protein, identified as lymphocyte expansion molecule (LEM). Interestingly, aug ...
... group identified mice with enhanced CD8 T cell responses to viral and tumor challenge (Okoye et al., 2015). The source of the heightened immunity gained after germline mutagenesis was the increased expression of an orphan protein, identified as lymphocyte expansion molecule (LEM). Interestingly, aug ...
Cadherins constitute a superfamily of transmembrane - HAL
... between cadherins exposed from opposing cells [13]. In addition to these structural data, EC1 domain emerges as the determinant area involved in the specific recognition of classical cadherins [14,16]. VE cadherin hexamer and artificial VE-cadherin based junctions Junctional assembly is a complex pr ...
... between cadherins exposed from opposing cells [13]. In addition to these structural data, EC1 domain emerges as the determinant area involved in the specific recognition of classical cadherins [14,16]. VE cadherin hexamer and artificial VE-cadherin based junctions Junctional assembly is a complex pr ...
Prostate Cancer Metastasis - The new web address for this page is
... The Garvan Institute of Medical Research (GIMR) is one of the largest independent medical research institutes in Australia, and forms part of the St Vincent’s Research Precinct in Darlinghurst, Sydney. The Institute was incorporated as an autonomous, non-profit research institute by the NSW Garvan I ...
... The Garvan Institute of Medical Research (GIMR) is one of the largest independent medical research institutes in Australia, and forms part of the St Vincent’s Research Precinct in Darlinghurst, Sydney. The Institute was incorporated as an autonomous, non-profit research institute by the NSW Garvan I ...
DRP1 family in cytokinesis and cell expansion
... sucrose-rescued adl1A mutants became photosynthetically active. These observations suggested that another functionally redundant member of the ADL1 family could compensate for the loss of ADL1A. Consistent with this finding, RNA gel blot analysis demonstrated that ADL1A, ADL1C, and ADL1E, but not AD ...
... sucrose-rescued adl1A mutants became photosynthetically active. These observations suggested that another functionally redundant member of the ADL1 family could compensate for the loss of ADL1A. Consistent with this finding, RNA gel blot analysis demonstrated that ADL1A, ADL1C, and ADL1E, but not AD ...
Nutrients and growth factors in mTORC1 activation
... form of RagA protein is constitutively bound to GTP (RagAGTP ) and allowed us generate mice with constitutive nutrient-dependent activation of mTORC1 in every cell. We first obtained MEFs (mouse embryonic fibroblasts) either RagA + / + or that harboured one copy of the RagAGTP allele (RagAGTP/ + ), ...
... form of RagA protein is constitutively bound to GTP (RagAGTP ) and allowed us generate mice with constitutive nutrient-dependent activation of mTORC1 in every cell. We first obtained MEFs (mouse embryonic fibroblasts) either RagA + / + or that harboured one copy of the RagAGTP allele (RagAGTP/ + ), ...