Materials - HAL
... and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift of Prof. C.H. Heldin) as a template in SacII-Bsu15I sites of modified pHM6 vector. SacII site was introduced by PCR in frame with ...
... and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift of Prof. C.H. Heldin) as a template in SacII-Bsu15I sites of modified pHM6 vector. SacII site was introduced by PCR in frame with ...
Light Chain λ and Ig κ Immature B Cell Stage in Mice Without Ig
... mouse there are an extensive number of V gene segments upstream of five J and one C gene (2). The mouse L chain locus contains within an ⬃200-kb region 2 sets of V, J, and C genes that can independently rearrange: V2-Vx-J2-C2-J4-C4 and V1-J3-C3J1-C1 (3). C1 appears to be predominantly express ...
... mouse there are an extensive number of V gene segments upstream of five J and one C gene (2). The mouse L chain locus contains within an ⬃200-kb region 2 sets of V, J, and C genes that can independently rearrange: V2-Vx-J2-C2-J4-C4 and V1-J3-C3J1-C1 (3). C1 appears to be predominantly express ...
hos1 - Oxford Academic - Oxford University Press
... the components and correct functioning of the clock itself play an important role in gating temperature signal transduction (Thomashow, 2010; Keily et al., 2013). Therefore, although it is common to compartmentalize phenotypes and functions within one pathway or another, it is important to consider ...
... the components and correct functioning of the clock itself play an important role in gating temperature signal transduction (Thomashow, 2010; Keily et al., 2013). Therefore, although it is common to compartmentalize phenotypes and functions within one pathway or another, it is important to consider ...
Global Gene Expression Responses of Fission Yeast to Ionizing
... and Pap1 (Toone et al., 1998). Deletion of these transcription factors results in altered sensitivity to a subset of stresses (Toda et al., 1991; Shiozaki and Russell, 1995; Degols and Russell, 1997; Nguyen et al., 2000; Quinn et al., 2002), indicating a role for these factors for modulating specifi ...
... and Pap1 (Toone et al., 1998). Deletion of these transcription factors results in altered sensitivity to a subset of stresses (Toda et al., 1991; Shiozaki and Russell, 1995; Degols and Russell, 1997; Nguyen et al., 2000; Quinn et al., 2002), indicating a role for these factors for modulating specifi ...
Myc Requires Distinct E2F Activities to Induce S Phase
... essentially abolished. As one further control, we also assayed the ability of E2F4⫺/⫺ cells to respond to Myc, since E2F4 is not normally induced by Myc expression. As shown in Figure 2, the E2F4⫺/⫺ cells responded equally well to Myc expression as did wild-type cells. Western blot assays demonstrat ...
... essentially abolished. As one further control, we also assayed the ability of E2F4⫺/⫺ cells to respond to Myc, since E2F4 is not normally induced by Myc expression. As shown in Figure 2, the E2F4⫺/⫺ cells responded equally well to Myc expression as did wild-type cells. Western blot assays demonstrat ...
A parafusin-related Toxoplasma protein in Ca -regulated secretory organelles
... geographical and host range distribution. T. gondii is associated with severe birth defects and with opportunistic infections in AIDS patients. T. gondii contains three types of secretory organelles: micronemes, rhoptries and dense granules (Ngo et al., 2000). Secretion from these organelles during ...
... geographical and host range distribution. T. gondii is associated with severe birth defects and with opportunistic infections in AIDS patients. T. gondii contains three types of secretory organelles: micronemes, rhoptries and dense granules (Ngo et al., 2000). Secretion from these organelles during ...
Structural and enzymatic characterization of a glycoside hydrolase
... cellulosome complexes [7], but are instead either secreted into the medium or attached to the cell membrane as lipoproteins. Indeed, approximately one-third of the encoded carbohydrate-degrading enzymes have been predicted to be lipoproteins [6], which may suggest that C. japonicus has developed a s ...
... cellulosome complexes [7], but are instead either secreted into the medium or attached to the cell membrane as lipoproteins. Indeed, approximately one-third of the encoded carbohydrate-degrading enzymes have been predicted to be lipoproteins [6], which may suggest that C. japonicus has developed a s ...
Intracellular distribution of histone mRNAs in human fibroblasts studied
... for poly(A)+ mRNA (Table 1). The distribution of actin mRNA was also evaluated in WI-38 cells and compared to the distribution of histone mRNA and poly(A)+ mRNA. Representative cells are shown in Figs. 5 and 6. Although actin mRNA was broadly distributed throughout the cytoplasm of most cells, it wa ...
... for poly(A)+ mRNA (Table 1). The distribution of actin mRNA was also evaluated in WI-38 cells and compared to the distribution of histone mRNA and poly(A)+ mRNA. Representative cells are shown in Figs. 5 and 6. Although actin mRNA was broadly distributed throughout the cytoplasm of most cells, it wa ...
Lenti-X™ Tet-On® Advanced Expression System User
... • Highly specific. The binding of the Tet-Advanced TetR-derived domains to the tetO target sequences of PTight is highly specific and does not activate off-target cellular genes. This high degree of specificity may be due in part to the prokaryotic nature of these components acting within the conte ...
... • Highly specific. The binding of the Tet-Advanced TetR-derived domains to the tetO target sequences of PTight is highly specific and does not activate off-target cellular genes. This high degree of specificity may be due in part to the prokaryotic nature of these components acting within the conte ...
Stockholm University
... Not surprisingly, there are reports that synonymous codon changes can influence membrane protein overexpression levels. A 6- to 9-fold increase in expression was observed when genes for the GluCl and GluCl ion channels from C. elegans were codon optimized and expressed in E18 rat hippocampal neuro ...
... Not surprisingly, there are reports that synonymous codon changes can influence membrane protein overexpression levels. A 6- to 9-fold increase in expression was observed when genes for the GluCl and GluCl ion channels from C. elegans were codon optimized and expressed in E18 rat hippocampal neuro ...
ChChd3, an Inner Mitochondrial Membrane Protein, Is
... cysteines arranged in CX9C or CX3C manner, and in the functional mature protein the cysteines are known to be oxidized forming two intramolecular disulfide bonds, essential for the proper folding and structural stabilization of the protein. The mitochondrial disulfide relay system mediated by Mia40 ...
... cysteines arranged in CX9C or CX3C manner, and in the functional mature protein the cysteines are known to be oxidized forming two intramolecular disulfide bonds, essential for the proper folding and structural stabilization of the protein. The mitochondrial disulfide relay system mediated by Mia40 ...
The human RNA-binding protein RBFA promotes the maturation of
... fractionation. The distribution of mt-SSU and mt-LSU protein components was visualized by western blot and RBFA was found to co-migrate with mt-SSU proteins (Fig 1D; mS29, mS40). To distinguish more accurately which mitoribosomal components RBFA associates with, a FLAG-tagged mitoribosomal protein ( ...
... fractionation. The distribution of mt-SSU and mt-LSU protein components was visualized by western blot and RBFA was found to co-migrate with mt-SSU proteins (Fig 1D; mS29, mS40). To distinguish more accurately which mitoribosomal components RBFA associates with, a FLAG-tagged mitoribosomal protein ( ...
15. Nervous System: Autonomic Nervous System
... NE to these alpha receptors causes vasoconstriction. Beta receptors are found in bronchioles of the lungs and blood vessels that serve the heart and skeletal muscles. Binding of NE to these beta receptors causes dilation. Beta receptors on cardiac muscle cause increases in heart rate and strength of ...
... NE to these alpha receptors causes vasoconstriction. Beta receptors are found in bronchioles of the lungs and blood vessels that serve the heart and skeletal muscles. Binding of NE to these beta receptors causes dilation. Beta receptors on cardiac muscle cause increases in heart rate and strength of ...
Localization of Low-sulfur Keratin Proteins in the Wool Follicle Using
... physical properties of keratin proteins have been defined in keratins extracted from wool fibers (13), almost all of the immunological studies have used either keratin extracted from epidermis (1, 15, 22, 29, 30) or cytokeratin (3, 4, 12, 15, 21). Therefore, there is a need to add an immunological a ...
... physical properties of keratin proteins have been defined in keratins extracted from wool fibers (13), almost all of the immunological studies have used either keratin extracted from epidermis (1, 15, 22, 29, 30) or cytokeratin (3, 4, 12, 15, 21). Therefore, there is a need to add an immunological a ...
Cholesterol and bile acids regulate xenosensor signaling in
... chicken, respectively (4-6,9). In mammals, CAR and PXR exhibit overlapping substrate and DNArecognition specificity and the exact contribution of these two receptors to drug-induction has not been fully elucidated (10-13). In chicken, only one xenobiotic-sensing orphan nuclear receptor has been iden ...
... chicken, respectively (4-6,9). In mammals, CAR and PXR exhibit overlapping substrate and DNArecognition specificity and the exact contribution of these two receptors to drug-induction has not been fully elucidated (10-13). In chicken, only one xenobiotic-sensing orphan nuclear receptor has been iden ...
Theoretical studies of Membrane Proteins
... this thesis. These proteins are present in the plasma membrane of prokaryotes and in all membranes of eukaryotic cells. In the rest of this thesis they will be referred to simply as membrane proteins. A helix bundle integral membrane protein is built up from α-helices that span the lipid bilayer, co ...
... this thesis. These proteins are present in the plasma membrane of prokaryotes and in all membranes of eukaryotic cells. In the rest of this thesis they will be referred to simply as membrane proteins. A helix bundle integral membrane protein is built up from α-helices that span the lipid bilayer, co ...
Molecular Chaperones - Cellular Machines for Protein Folding
... The low specificity of the hydrophobic interaction and the conformational flexibility of folding intermediates ensures that chaperones act promiscuously: they bind to a large variety of polypeptides that differ widely in amino acid sequence and in conformation. However, since most native proteins an ...
... The low specificity of the hydrophobic interaction and the conformational flexibility of folding intermediates ensures that chaperones act promiscuously: they bind to a large variety of polypeptides that differ widely in amino acid sequence and in conformation. However, since most native proteins an ...
So What`s New in the Field of Plant Cold
... phosphatase 2A activity that is dependent on calcium influx (17). Thus, low temperature may lead to an influx in calcium that inhibits phosphatase 2A activity that, in turn, leads to the phosphorylation of one or more proteins involved in inducing genes involved in cold acclimation. The protein kina ...
... phosphatase 2A activity that is dependent on calcium influx (17). Thus, low temperature may lead to an influx in calcium that inhibits phosphatase 2A activity that, in turn, leads to the phosphorylation of one or more proteins involved in inducing genes involved in cold acclimation. The protein kina ...
Expip is a cargo adaptor for Sec24p ... export the plasma membrane H+ ATPase from the
... Overview of the secretory pathway Translocation into the ER Cargo proteins of the secretory pathway enter the pathway at the ER, the surface of which is coated with ribosomes that allow coupling of protein translation with translocation into the ER. As newly synthesized proteins emerge from the rib ...
... Overview of the secretory pathway Translocation into the ER Cargo proteins of the secretory pathway enter the pathway at the ER, the surface of which is coated with ribosomes that allow coupling of protein translation with translocation into the ER. As newly synthesized proteins emerge from the rib ...
Cells as Tensegrity Structures: Architectural Basis of the Cytoskeleton
... this buckling could balance a substantial fraction of the contractile prestress. To investigate this possibility, we carried out an energetic analysis of buckling of microtubules [46]. The assumption was that energy stored in microtubules during compression is transferred to a flexible substrate upo ...
... this buckling could balance a substantial fraction of the contractile prestress. To investigate this possibility, we carried out an energetic analysis of buckling of microtubules [46]. The assumption was that energy stored in microtubules during compression is transferred to a flexible substrate upo ...
Differential Localization of G Protein βγ Subunits
... isoform specific antibodies. Studies examining synaptic ...
... isoform specific antibodies. Studies examining synaptic ...
Shock
... thus making them Gram positive bacteria – Staphylococcus and Streptococcus. • Some bacteria have a membrane outside the cell wall (thus they have a cell membrane, cell wall and outer membrane). The outer membrane is made up of Lipopolysaccharides. This does not fix the gram stain thus making them Gr ...
... thus making them Gram positive bacteria – Staphylococcus and Streptococcus. • Some bacteria have a membrane outside the cell wall (thus they have a cell membrane, cell wall and outer membrane). The outer membrane is made up of Lipopolysaccharides. This does not fix the gram stain thus making them Gr ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.