Read about actions of formaldehyde on antigens
... can be detected only in unfixed cryosections; most are more easily detected after fixation. Paraffin embedding is usually preferable to either plastic embedding or cutting unembedded specimens with a vibrating microtome or in a cryostat. There are, however, plenty of exceptions to this generalizatio ...
... can be detected only in unfixed cryosections; most are more easily detected after fixation. Paraffin embedding is usually preferable to either plastic embedding or cutting unembedded specimens with a vibrating microtome or in a cryostat. There are, however, plenty of exceptions to this generalizatio ...
THE GENE THE EVIDENCE OF THE NUCLEOPROTEIN NATURE OF
... the specificities of the different regions as well. Actually what results is that in salivary gland chromosomes of Drosophila melanogaster, evidence is obtained for the presence of two and possibly three types of proteins; a protein resembling in its absorption characteristics the serum globulins, o ...
... the specificities of the different regions as well. Actually what results is that in salivary gland chromosomes of Drosophila melanogaster, evidence is obtained for the presence of two and possibly three types of proteins; a protein resembling in its absorption characteristics the serum globulins, o ...
Corticotropin-releasing hormone induces vascular endothelial
... either preformed mediators or cytokines. This effect was mediated through activation of CRH receptor-1 and adenylate cyclase with increased intracellular cAMP. However, the precise mechanism by which CRH induces VEGF secretion has not yet been defined. Here, we show that CRH-induced VEGF release was ...
... either preformed mediators or cytokines. This effect was mediated through activation of CRH receptor-1 and adenylate cyclase with increased intracellular cAMP. However, the precise mechanism by which CRH induces VEGF secretion has not yet been defined. Here, we show that CRH-induced VEGF release was ...
Inducible Gene Targeting in Postnatal Myocardium by Cardiac
... Cre/loxP system derived from bacteriophage P1.2,3 The 38kDa Cre protein functions as a site-specific recombinase, splicing DNA between specific 34-bp sequences known as loxP sites. Mating mice that contain a loxP-flanked gene with mice expressing Cre under the control of a heart-specific promoter re ...
... Cre/loxP system derived from bacteriophage P1.2,3 The 38kDa Cre protein functions as a site-specific recombinase, splicing DNA between specific 34-bp sequences known as loxP sites. Mating mice that contain a loxP-flanked gene with mice expressing Cre under the control of a heart-specific promoter re ...
Slide 1
... Source: Modeling Future Heroes, A Practical Application of Heroic Values, By Roger F. Cram Source: NAMI–Family to Family Course, Class 6, Handout 2–Basic Neuro-transmission at the Synapse–page 6.23 Paragraph 3 ...
... Source: Modeling Future Heroes, A Practical Application of Heroic Values, By Roger F. Cram Source: NAMI–Family to Family Course, Class 6, Handout 2–Basic Neuro-transmission at the Synapse–page 6.23 Paragraph 3 ...
Photoactivation of GFP reveals protein dynamics within the
... Received 31 May 2005; Accepted 15 August 2005 ...
... Received 31 May 2005; Accepted 15 August 2005 ...
Drug-Resistant Variants of Escherichia coli Thymidylate Synthase
... Inhibition of TS results in apoptotic cell death that is caused by intracellular thymidine depletion. Acquired resistance to TS inhibitors is a multifactorial process that can include amplification and/or mutation of the TS gene. In cells adapted to high concentrations of FdUrd, we showed that TS ge ...
... Inhibition of TS results in apoptotic cell death that is caused by intracellular thymidine depletion. Acquired resistance to TS inhibitors is a multifactorial process that can include amplification and/or mutation of the TS gene. In cells adapted to high concentrations of FdUrd, we showed that TS ge ...
Systematic comparison of single-chain Fv antibody
... review see [3-5]) which are not only easier to manipulate but which also yield ITs endowed with consistent physico-chemical properties. In particular, toxic enzymatic sequences can be directly genetically fused to sequences encoding the selected targeting domains (e.g. hormones, growth factors, anti ...
... review see [3-5]) which are not only easier to manipulate but which also yield ITs endowed with consistent physico-chemical properties. In particular, toxic enzymatic sequences can be directly genetically fused to sequences encoding the selected targeting domains (e.g. hormones, growth factors, anti ...
Identification, Expression and Characterization of Archaeal
... photoreceptors in natural and artificial systems” and Institute für Biochemie-I of university of Regensburg, Germany. I thank DFG and SFB for providing me fellowship for four years. I thank all my lab members, M. Fuhrmann, M. Heitzer, C. Griesbeck, A. Stahlberg, S. Ehlenbeck, P. Berthold, T. Schiere ...
... photoreceptors in natural and artificial systems” and Institute für Biochemie-I of university of Regensburg, Germany. I thank DFG and SFB for providing me fellowship for four years. I thank all my lab members, M. Fuhrmann, M. Heitzer, C. Griesbeck, A. Stahlberg, S. Ehlenbeck, P. Berthold, T. Schiere ...
Emerging regulatory mechanisms in ubiquitin
... genomic material, correct mistakes made by sloppy DNA polymerases, repair damage caused by harsh environments and yet still distribute their chromosomes into identical daughter cells. Errors in this program can be deadly for the cell, or, if they result in transformation, have detrimental effects on ...
... genomic material, correct mistakes made by sloppy DNA polymerases, repair damage caused by harsh environments and yet still distribute their chromosomes into identical daughter cells. Errors in this program can be deadly for the cell, or, if they result in transformation, have detrimental effects on ...
Document
... Gel Filtration Resin. When starting protein purification, technicians sometimes use a gel-filtration (size-exclusion) column first. They know the molecular weight of their protein, so they can often eliminate several contaminant proteins by a quick run through a sizing column. ...
... Gel Filtration Resin. When starting protein purification, technicians sometimes use a gel-filtration (size-exclusion) column first. They know the molecular weight of their protein, so they can often eliminate several contaminant proteins by a quick run through a sizing column. ...
Conformational flexibility may explain multiple cellular roles of PEST
... motifs in eukaryotic proteomes. PEST motifs were found to be overrepresented in the proteins belonging to nucleic acid and protein binding, transcription regulation, and signal transduction classes.15 They were also found to be surface exposed, enriched in characterized disordered protein database, ...
... motifs in eukaryotic proteomes. PEST motifs were found to be overrepresented in the proteins belonging to nucleic acid and protein binding, transcription regulation, and signal transduction classes.15 They were also found to be surface exposed, enriched in characterized disordered protein database, ...
Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and
... organ size, as does ARGOS, we generated transgenic 35SOSR1 plants and examined their organ development. Compared with control lines, which contained an empty vector, all of 20 independently transgenic lines overexpressing OSR1 displayed visibly enlarged aerial organs, including leaves, cotyledons, a ...
... organ size, as does ARGOS, we generated transgenic 35SOSR1 plants and examined their organ development. Compared with control lines, which contained an empty vector, all of 20 independently transgenic lines overexpressing OSR1 displayed visibly enlarged aerial organs, including leaves, cotyledons, a ...
Dorsal–ventral patterning: a view from the top - ICB-USP
... dIs [19,20]. These studies have led to the idea that dIs remain plastic in their fates even after they have exited the cell cycle, a property that is shared by ventrally located motor neurons that depend on HD proteins for their specification [2]. They also raise the possibility that similar postmit ...
... dIs [19,20]. These studies have led to the idea that dIs remain plastic in their fates even after they have exited the cell cycle, a property that is shared by ventrally located motor neurons that depend on HD proteins for their specification [2]. They also raise the possibility that similar postmit ...
Hydraulic and chemical signalling in the control of stomatal
... stomatal pore is actually achieved by turgor regulation in the guard cell, these are generally actively controlled processes of osmotic regulation and not passive consequences of bulk tissue water potential (Buckley and Mott, 2001). Tremendous importance has therefore been placed on understanding th ...
... stomatal pore is actually achieved by turgor regulation in the guard cell, these are generally actively controlled processes of osmotic regulation and not passive consequences of bulk tissue water potential (Buckley and Mott, 2001). Tremendous importance has therefore been placed on understanding th ...
Full-Text PDF
... target them to the lysosomal pathway; the degradation of pathogens through the autophagy pathway is known as xenophagy [2]. However, most infectious pathogens have evolved strategies to escape autophagy or even to use some properties of this cellular mechanism to optimize their intracellular life cy ...
... target them to the lysosomal pathway; the degradation of pathogens through the autophagy pathway is known as xenophagy [2]. However, most infectious pathogens have evolved strategies to escape autophagy or even to use some properties of this cellular mechanism to optimize their intracellular life cy ...
A Tertiary Plastid Uses Genes from Two Endosymbionts
... within the host endomembrane system, so plastidtargeting leaders consist of a signal peptide (SP) followed by a TP. The SP directs the proteins to the endomembrane system via the signal recognition particle, and from there the TP directs them to the plastid.6 One algal group that contains a secondar ...
... within the host endomembrane system, so plastidtargeting leaders consist of a signal peptide (SP) followed by a TP. The SP directs the proteins to the endomembrane system via the signal recognition particle, and from there the TP directs them to the plastid.6 One algal group that contains a secondar ...
1495/Chapter 03
... membrane, separated by an intermembrane space, as shown in Figure 3.7. The inner membrane folds as shelf-like cristae and contains the matrix, an enzyme-rich fluid. The cristae and the matrix are the sites where ATP synthesis occurs. More mitochondria are found in cells that require more energy, suc ...
... membrane, separated by an intermembrane space, as shown in Figure 3.7. The inner membrane folds as shelf-like cristae and contains the matrix, an enzyme-rich fluid. The cristae and the matrix are the sites where ATP synthesis occurs. More mitochondria are found in cells that require more energy, suc ...
The Arabidopsis Callose Synthase Gene GSL8 Is
... functions in callose deposition after injury and pathogen treatment is GSL5 (Jacobs et al., 2003). Five other members of the Arabidopsis GSL family are required for microgametogenesis. GSL1 and GSL5 act redundantly to produce a callosic wall that prevents microspore degeneration, and both are needed ...
... functions in callose deposition after injury and pathogen treatment is GSL5 (Jacobs et al., 2003). Five other members of the Arabidopsis GSL family are required for microgametogenesis. GSL1 and GSL5 act redundantly to produce a callosic wall that prevents microspore degeneration, and both are needed ...
Integrating Optogenetic and Pharmacological Approaches to Study
... 2010; Yizhar et al., 2011b; Marin, 2012). More specifically, dysfunctional circuit mechanisms within the FS interneurons that selectively express the calciumbinding protein, parvalbumin, are hypothesized to underlie a range of symptoms of these neuropsychiatric diseases. Optogenetic manipulations ha ...
... 2010; Yizhar et al., 2011b; Marin, 2012). More specifically, dysfunctional circuit mechanisms within the FS interneurons that selectively express the calciumbinding protein, parvalbumin, are hypothesized to underlie a range of symptoms of these neuropsychiatric diseases. Optogenetic manipulations ha ...
Control of cell differentiation and morphogenesis in amphibian
... in unfertilized eggs and blastulae of Xenopus has been demonstrated (Asashima el al., 1991b) even before the 16-cell stage of early embryos (Fukui et al.. 1994). Activin homologs are, indeed, contained in an egg of Xenopus laevis in a considerable amount (about 1 pg/egg) as a maternal protein. These ...
... in unfertilized eggs and blastulae of Xenopus has been demonstrated (Asashima el al., 1991b) even before the 16-cell stage of early embryos (Fukui et al.. 1994). Activin homologs are, indeed, contained in an egg of Xenopus laevis in a considerable amount (about 1 pg/egg) as a maternal protein. These ...
The transcription factor AtDOF4.2 regulates shoot branching and
... DOF), HvDOF17 and HvDOF19 control seed germination by affecting the expression of different aleurone hydrolase genes [7–9]. DOF proteins from Arabidopsis have been found to be mediators of many biological processes, including the light response [10], flowering [11], plant growth [12,13], hormone res ...
... DOF), HvDOF17 and HvDOF19 control seed germination by affecting the expression of different aleurone hydrolase genes [7–9]. DOF proteins from Arabidopsis have been found to be mediators of many biological processes, including the light response [10], flowering [11], plant growth [12,13], hormone res ...
Heat stress-induced localization of small heat shock
... Lamin A/C speckles have earlier been shown to colocalize with mRNA splicing factors such as SC-35 [29] and mediate spatial organization of SFCs [39]. We have investigated whether aB-crystallin also colocalizes with SC-35 in normal and heat-stressed myoblasts. Fig. 3 (unstressed cells) shows that aB- ...
... Lamin A/C speckles have earlier been shown to colocalize with mRNA splicing factors such as SC-35 [29] and mediate spatial organization of SFCs [39]. We have investigated whether aB-crystallin also colocalizes with SC-35 in normal and heat-stressed myoblasts. Fig. 3 (unstressed cells) shows that aB- ...
Protein Kinase C inhibits Kv1.1 potassium channel function
... in ventricular cells (36). In the Xenopus oocyte expression system, several types of recombinant, voltagegated K channels have been shown to be modulated by PKC (18, 19, 21, 23, 29), and in some cases there is evidence that the K channel protein is the substrate for PKC phosphorylation (2, 3, 4, 8). ...
... in ventricular cells (36). In the Xenopus oocyte expression system, several types of recombinant, voltagegated K channels have been shown to be modulated by PKC (18, 19, 21, 23, 29), and in some cases there is evidence that the K channel protein is the substrate for PKC phosphorylation (2, 3, 4, 8). ...
Neisseria conserved hypothetical protein DMP12
... Figure 2. The crystal structure of DMP12 presents a DNA-like surface. (A) The secondary structural elements are shown above the amino acid sequence, with blue cylinders representing a-helices and green arrows representing b-sheets. (B) A ribbon diagram of two DMP12 monomers in an asymmetric unit. Th ...
... Figure 2. The crystal structure of DMP12 presents a DNA-like surface. (A) The secondary structural elements are shown above the amino acid sequence, with blue cylinders representing a-helices and green arrows representing b-sheets. (B) A ribbon diagram of two DMP12 monomers in an asymmetric unit. Th ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.