Pseudomonas aeruginosa Anaerobic Respiration in Biofilms
... the anaerobic ⌬rhlR biofilm bacteria were dead, consistent with the results described above. In contrast, the majority of the rhlRnirS double mutants, which lack the only enzyme that produces NO (nitrite reductase) in P. aeruginosa, were alive. In addition, other mutants deficient in nitrite reducta ...
... the anaerobic ⌬rhlR biofilm bacteria were dead, consistent with the results described above. In contrast, the majority of the rhlRnirS double mutants, which lack the only enzyme that produces NO (nitrite reductase) in P. aeruginosa, were alive. In addition, other mutants deficient in nitrite reducta ...
Characterization of Agouti-Related Protein
... Previous biochemical studies of Agouti protein have been complicated by the lack of a radiolabeled derivative. In contrast, AGRP has two tyrosine residues, both of which are present in its carboxyl-terminal sequence. We were therefore able to take advantage of ...
... Previous biochemical studies of Agouti protein have been complicated by the lack of a radiolabeled derivative. In contrast, AGRP has two tyrosine residues, both of which are present in its carboxyl-terminal sequence. We were therefore able to take advantage of ...
Clostridium difficile glutamate dehydrogenase is a
... after 10 s shaking of the plate. To measure the bacterial growth in the presence of H2O2, bacterial strains were grown in TY medium with 50 mM H2O2 and the OD600 was measured at regular intervals using a Bioscreen C plate reader. Bacterial survival upon exposure to H2O2. Bacterial cultures ...
... after 10 s shaking of the plate. To measure the bacterial growth in the presence of H2O2, bacterial strains were grown in TY medium with 50 mM H2O2 and the OD600 was measured at regular intervals using a Bioscreen C plate reader. Bacterial survival upon exposure to H2O2. Bacterial cultures ...
PDF - Oxford Academic - Oxford University Press
... ear are shorter than normal, whereas in low-frequency regions, dendrites of deprived neurons are longer than normal (Smith et al., 1983). Thus, dendritic structure is rapidly responsive to changes in afferent activity. Increasing afferent activity by raising either young or adult rats in enriched en ...
... ear are shorter than normal, whereas in low-frequency regions, dendrites of deprived neurons are longer than normal (Smith et al., 1983). Thus, dendritic structure is rapidly responsive to changes in afferent activity. Increasing afferent activity by raising either young or adult rats in enriched en ...
Auxin coordinates cell division and cell fate specification during
... of certain cell types might be enhanced at well-defined stages during plant development (Thomas et al., 2003). Whatever the real auxin receptor(s) might be, new data on the potential involvement of GTP binding proteins in controlling the auxin sensitivity towards cell division ...
... of certain cell types might be enhanced at well-defined stages during plant development (Thomas et al., 2003). Whatever the real auxin receptor(s) might be, new data on the potential involvement of GTP binding proteins in controlling the auxin sensitivity towards cell division ...
Auxin coordinates cell division and cell fate specification during
... of certain cell types might be enhanced at well-defined stages during plant development (Thomas et al., 2003). Whatever the real auxin receptor(s) might be, new data on the potential involvement of GTP binding proteins in controlling the auxin sensitivity towards cell division ...
... of certain cell types might be enhanced at well-defined stages during plant development (Thomas et al., 2003). Whatever the real auxin receptor(s) might be, new data on the potential involvement of GTP binding proteins in controlling the auxin sensitivity towards cell division ...
Sorting and convergence of primary olfactory axons are
... localised? Recent analyses of mice with a reduced complement of mitral cells have suggested that these postsynaptic neurons are not involved in the sorting and convergence of primary olfactory axons to their topographic targets (Bulfone et al., 1998). However, it was unclear whether cues present on ...
... localised? Recent analyses of mice with a reduced complement of mitral cells have suggested that these postsynaptic neurons are not involved in the sorting and convergence of primary olfactory axons to their topographic targets (Bulfone et al., 1998). However, it was unclear whether cues present on ...
General principles of cellular organization in the genome
... structural models for 484 proteins, single particle EM and cellular electron tomograms provided supporting structural details for this proteome organization. To understand basic principles of bacterial metabolism and its regulation, a manually curated metabolic network of 129 enzymes catalyzing 189 ...
... structural models for 484 proteins, single particle EM and cellular electron tomograms provided supporting structural details for this proteome organization. To understand basic principles of bacterial metabolism and its regulation, a manually curated metabolic network of 129 enzymes catalyzing 189 ...
Identification and characterization of novel interaction
... Immobilization of CcpA via Avi-tag on an SPR chip ................................................................... 70 ...
... Immobilization of CcpA via Avi-tag on an SPR chip ................................................................... 70 ...
Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for
... Despite these recent advances, one area of active research is the identification of dynein-associated proteins contributing to its checkpoint function. One protein shown to physically interact with dynein is the WD40 repeat containing Lis1 protein (Faulkner et al., 2000; Smith et al., 2000; Tai et a ...
... Despite these recent advances, one area of active research is the identification of dynein-associated proteins contributing to its checkpoint function. One protein shown to physically interact with dynein is the WD40 repeat containing Lis1 protein (Faulkner et al., 2000; Smith et al., 2000; Tai et a ...
What recent ribosome structures have revealed
... the template and aminoacyl-transfer RNAs as substrates. Ribosomes from bacteria consist of a large (50S) and a small (30S) subunit, which together compose the 2.5-megadalton 70S ribosome; their eukaryotic counterparts are the 60S and 40S subunits and the 80S ribosome. The 50S subunit consists of 23S ...
... the template and aminoacyl-transfer RNAs as substrates. Ribosomes from bacteria consist of a large (50S) and a small (30S) subunit, which together compose the 2.5-megadalton 70S ribosome; their eukaryotic counterparts are the 60S and 40S subunits and the 80S ribosome. The 50S subunit consists of 23S ...
ASD v2.0: updated content and novel features
... Allostery is a fundamental process that regulates a protein’s functional activity through the induction of changes in its conformation and dynamics in response to the perturbation of an effector at a site distinct from the active site, also termed the allosteric site (1). The mechanisms of allosteri ...
... Allostery is a fundamental process that regulates a protein’s functional activity through the induction of changes in its conformation and dynamics in response to the perturbation of an effector at a site distinct from the active site, also termed the allosteric site (1). The mechanisms of allosteri ...
Cooperative and independent functions of FGF and Wnt signaling
... otic vesicles that are smaller than otic vesicles produced after inactivation of either wnt8b or fgf3 alone [22]. In mice, expression of Wnt8a in the hindbrain is reduced in Fgf3−/−; Fgf10−/− double mutant embryos and otic vesicles are reduced or absent [29]. Sprouty genes encode antagonists of rece ...
... otic vesicles that are smaller than otic vesicles produced after inactivation of either wnt8b or fgf3 alone [22]. In mice, expression of Wnt8a in the hindbrain is reduced in Fgf3−/−; Fgf10−/− double mutant embryos and otic vesicles are reduced or absent [29]. Sprouty genes encode antagonists of rece ...
concentration-dependent effects of neostigmine on the endplate
... the synaptic cleft of the motor endplate is subject to three main processes: (1) attachment to specific endplate receptors leading to a transient opening of ion gates, (2) attachment to acetylcholinesterase (AChE) leading to the hydrolysis of ACh, and (3) diffusional spread leading to escape from th ...
... the synaptic cleft of the motor endplate is subject to three main processes: (1) attachment to specific endplate receptors leading to a transient opening of ion gates, (2) attachment to acetylcholinesterase (AChE) leading to the hydrolysis of ACh, and (3) diffusional spread leading to escape from th ...
Characterization of Vincristine Transport by the Mr 190,000
... as leukotriene < ,. We also show that short-chain alkyl derivatives of GSH can stimulate drug uptake, which suggests that vincristine transport does not necessarily involve a change in redox state or glutathionylation of the protein. Furthermore, vincristine uptake is accompanied by ATP- and drug-de ...
... as leukotriene < ,. We also show that short-chain alkyl derivatives of GSH can stimulate drug uptake, which suggests that vincristine transport does not necessarily involve a change in redox state or glutathionylation of the protein. Furthermore, vincristine uptake is accompanied by ATP- and drug-de ...
The Major Component of the Paraflagellar Rod of Trypanosoma
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
... similarity to the intermediate filament proteins (solubility properties, amino acid composition, and high degree of helicity), the PFR protein does not belong in this class of cytoskeletal proteins. The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both gene ...
Profilin association with monomeric actin in
... antibody staining. Other than monomeric actin, no major profilin ligands are detected in crude extracts. Profilin-II labeled with rhodamine on cysteine at position 58 retains ...
... antibody staining. Other than monomeric actin, no major profilin ligands are detected in crude extracts. Profilin-II labeled with rhodamine on cysteine at position 58 retains ...
Macrophages from Vacuoles in Activated monocytogenes Listeria
... escapes into the macrophage cytoplasm. There it can grow, divide, and eventually nucleate host cell actin in a process that facilitates transfer to neighboring cells (1). However, if the macrophage is activated, by IFN-␥, bacterial products, and other cytokines produced during the immune response to ...
... escapes into the macrophage cytoplasm. There it can grow, divide, and eventually nucleate host cell actin in a process that facilitates transfer to neighboring cells (1). However, if the macrophage is activated, by IFN-␥, bacterial products, and other cytokines produced during the immune response to ...
REGENERATION OF AN IDENTIFIED CENTRAL NEURON IN THE
... duced beneath. With this procedure no nerves or trachnerves entering or leaving the terminal ganglion, such as eoles entering the ganglion were cut. For intracellular the cereal nerve or the central connectives. Within a few recordings from the MGI soma located on the lateral days post-axotomy, many ...
... duced beneath. With this procedure no nerves or trachnerves entering or leaving the terminal ganglion, such as eoles entering the ganglion were cut. For intracellular the cereal nerve or the central connectives. Within a few recordings from the MGI soma located on the lateral days post-axotomy, many ...
METABOLIC CUES AND REGULATORY PROTEINS
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
... macrophages to cause the severe pneumonia, Legionnaires’ disease. To persist within these diverse niches, L. pneumophila alternates between at least two distinct phenotypic phases: a non-infectious, replicative form required for intracellular growth and an infectious, transmissive form that enhances ...
presence of allhnase in isolated vacuoles and of alkyl
... alkyl cysteine sulphoxides. (The trace of alkyl cysteine sulphoxides was probably the result of the 1% or so contamination by intact protoplasts). The amount of alliinase in the vacuoles was sufficient to account for the alliinase in a similar number of intact protoplasts, suggesting that in the int ...
... alkyl cysteine sulphoxides. (The trace of alkyl cysteine sulphoxides was probably the result of the 1% or so contamination by intact protoplasts). The amount of alliinase in the vacuoles was sufficient to account for the alliinase in a similar number of intact protoplasts, suggesting that in the int ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
... In eukaryotic cells tRNAs are transcribed in the nucleus and exported to the cytoplasm for their essential role in protein synthesis. This export event was thought to be unidirectional. Surprisingly, several lines of evidence showed that mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm ...
Branching out: mechanisms of dendritic arborization
... Many transcription factors contribute to the specification of neuronal type-specific dendrite patterns. A distinct dendrite morphology can be achieved by varying the levels of a single transcription factor, the specific expression of a transcription factor in a single type of neuron and combinatoria ...
... Many transcription factors contribute to the specification of neuronal type-specific dendrite patterns. A distinct dendrite morphology can be achieved by varying the levels of a single transcription factor, the specific expression of a transcription factor in a single type of neuron and combinatoria ...
Auxin transport – shaping the plant
... underlies many adaptation processes including organogenesis, meristem patterning and tropisms. In executing its multiple roles, auxin displays some characteristics of both a hormone and a morphogen. Studies on auxin transport, as well as tracing the intracellular movement of its molecular components ...
... underlies many adaptation processes including organogenesis, meristem patterning and tropisms. In executing its multiple roles, auxin displays some characteristics of both a hormone and a morphogen. Studies on auxin transport, as well as tracing the intracellular movement of its molecular components ...
A storage form of ribosomes in mouse oocytes
... for making whole mount preparations or for of the beads averaged 212 A while the con embedding and sectioning. DNase had no nections between beads were approx. 1 25 A effect on the lattices, but they were readily in diameter. destroyed by either trypsin or RNase. In all Sometimes the chains existed ...
... for making whole mount preparations or for of the beads averaged 212 A while the con embedding and sectioning. DNase had no nections between beads were approx. 1 25 A effect on the lattices, but they were readily in diameter. destroyed by either trypsin or RNase. In all Sometimes the chains existed ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.