Defects in mesoderm, neural tube and vascular development in
... phenol/chloroform, once with chloroform and ethanol-precipitated. Embryos in paraffin sections were genotyped by scraping embryonic tissue from sections. Scrapings from several sections were pooled in 60 µl H 2O, incubated with 20 µg Proteinase K at 55°C for 30 minutes, 95°C for 10 minutes. Separate ...
... phenol/chloroform, once with chloroform and ethanol-precipitated. Embryos in paraffin sections were genotyped by scraping embryonic tissue from sections. Scrapings from several sections were pooled in 60 µl H 2O, incubated with 20 µg Proteinase K at 55°C for 30 minutes, 95°C for 10 minutes. Separate ...
Cagnac, O., Leterrier, M., Yeager, M. and Blumwald, E. (2007).
... with Mntp1p, a Golgi-specific marker (11). Although Kha1p displays high amino acid sequence similarity to other Na⫹/H⫹ antiporters, its ability to mediate K⫹/H⫹ exchange has not been yet demonstrated. Nhx1p localizes to the pre-vacuolar compartment and function in the sequestration of sodium ions (K ...
... with Mntp1p, a Golgi-specific marker (11). Although Kha1p displays high amino acid sequence similarity to other Na⫹/H⫹ antiporters, its ability to mediate K⫹/H⫹ exchange has not been yet demonstrated. Nhx1p localizes to the pre-vacuolar compartment and function in the sequestration of sodium ions (K ...
Phosphorylation-Dependent Regulation of Septin
... regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis. Introduction In order to give rise to two viable progenies, cytokinesis must be both spatially and temporally coordinated with the partition of sister chromatids ...
... regulation of septin dynamics, possibly through the control of their phosphorylation state, is required for the completion of cytokinesis. Introduction In order to give rise to two viable progenies, cytokinesis must be both spatially and temporally coordinated with the partition of sister chromatids ...
G1 Phase-Dependent Expression of Bcl
... PBS and reculture in the growth medium. At each time point, onethird of the cells were harvested for Western blotting assay, onethird for cell cycle analysis, and one-third were treated for 3 or 24 h with either etoposide (50 M/ml), cisplatin (20 M/ml), or staurosporin (1 M/ml). After each treatm ...
... PBS and reculture in the growth medium. At each time point, onethird of the cells were harvested for Western blotting assay, onethird for cell cycle analysis, and one-third were treated for 3 or 24 h with either etoposide (50 M/ml), cisplatin (20 M/ml), or staurosporin (1 M/ml). After each treatm ...
Thesis titel 1.5 final - SciDok
... Chorio-Alantoic Membrane (CAM), which is crucial for embryonic growth and development. Conclusion: This thesis is the first step towards the overall aim t develop an artificial egg as in vitro cell culture system and gives a highly important insight into its feasibility. The results of this thesis i ...
... Chorio-Alantoic Membrane (CAM), which is crucial for embryonic growth and development. Conclusion: This thesis is the first step towards the overall aim t develop an artificial egg as in vitro cell culture system and gives a highly important insight into its feasibility. The results of this thesis i ...
Programmed cell death in C. elegans: the genetic framework
... C. elegans is a free living worm that feeds on bacteria and can be easily maintained in the laboratory (1). The rapid life cycle of C. elegans (about 2.5 days at 25oC), the self-fertilizing ability of its hermaphrodite, and its well-defined anatomy have made it an ideal organism for sophisticated ge ...
... C. elegans is a free living worm that feeds on bacteria and can be easily maintained in the laboratory (1). The rapid life cycle of C. elegans (about 2.5 days at 25oC), the self-fertilizing ability of its hermaphrodite, and its well-defined anatomy have made it an ideal organism for sophisticated ge ...
S3.03 The maximum degree of local invasion into or through the
... breached the serosal elastic lamina but not appear on the surface of the colon as it elicits a fibroblastic reaction that forms a cap over the tumour. This finding appears to have adverse significance and tumours showing this feature may have the significance of a pT4a stage. An elastic tissue stain ...
... breached the serosal elastic lamina but not appear on the surface of the colon as it elicits a fibroblastic reaction that forms a cap over the tumour. This finding appears to have adverse significance and tumours showing this feature may have the significance of a pT4a stage. An elastic tissue stain ...
The Regulation of S Phase Initiation by p27Kip1 in NIH3T3 Cells
... cyclin D1 levels are normally induced,1 while p27Kip1 (p27) levels are suppressed.2 These two molecular events then directly influence the activity of key cell cycle regulatory proteins. Cyclin D1 associated with cyclin dependent kinase (CDK) 4 or 6 inactivates the growth inhibitory retinoblastoma p ...
... cyclin D1 levels are normally induced,1 while p27Kip1 (p27) levels are suppressed.2 These two molecular events then directly influence the activity of key cell cycle regulatory proteins. Cyclin D1 associated with cyclin dependent kinase (CDK) 4 or 6 inactivates the growth inhibitory retinoblastoma p ...
TGLN Manual - Trillium Gift of Life Network
... Trillium Gift of Life Network (TGLN) is committed to creating a culture that enables every Ontarian to make an informed decision about organ and tissue donation and to support healthcare professionals in implementing those decisions. This resource manual was developed by TGLN as a tool to support he ...
... Trillium Gift of Life Network (TGLN) is committed to creating a culture that enables every Ontarian to make an informed decision about organ and tissue donation and to support healthcare professionals in implementing those decisions. This resource manual was developed by TGLN as a tool to support he ...
Bacterial cheating drives the population dynamics of
... death, and cost associated with resistance (Supplementary information). In particular, our analytic solution of the model predicts that the resistant fraction at equilibrium increases approximately linearly with the antibiotic concentration, a prediction borne out in experimental difference maps obt ...
... death, and cost associated with resistance (Supplementary information). In particular, our analytic solution of the model predicts that the resistant fraction at equilibrium increases approximately linearly with the antibiotic concentration, a prediction borne out in experimental difference maps obt ...
Distribution of polycyclic aromatic hydrocarbons in
... forms with minimum input of chemicals and energy [7,12-15]. Previous studies have shown the efficacy of plant uptake and metabolism of PAHs in removing PAHs from the environment [16-18]. In general, two primary processes are responsible for PAH transfer and distribution in plant tissues: (1) transfe ...
... forms with minimum input of chemicals and energy [7,12-15]. Previous studies have shown the efficacy of plant uptake and metabolism of PAHs in removing PAHs from the environment [16-18]. In general, two primary processes are responsible for PAH transfer and distribution in plant tissues: (1) transfe ...
antinuclear antibodies - Annals of the Rheumatic Diseases
... also show speckled IgG depVosits in the cytoplasm of epidermal cells (fig 2). In general, speckled ANA patterns were found significantly more often in skin than in serum, whereas the homogeneous pattern was found more commonly in serum than in skin. This may be the result of incomplete dilution of t ...
... also show speckled IgG depVosits in the cytoplasm of epidermal cells (fig 2). In general, speckled ANA patterns were found significantly more often in skin than in serum, whereas the homogeneous pattern was found more commonly in serum than in skin. This may be the result of incomplete dilution of t ...
Copyright Information of the Article Published Online
... © The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. Core tip: The levels of CD44 and miR-145 are related strongly to stemness properties in gastric cancer. The aim of this investigation was to determine the underlying molecular mechanism involved in this relation ...
... © The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. Core tip: The levels of CD44 and miR-145 are related strongly to stemness properties in gastric cancer. The aim of this investigation was to determine the underlying molecular mechanism involved in this relation ...
The effect of Centella asiatica, vitamins, glycolic acid and their
... The effect of Centella asiatica, vitamins, glycolic acid and their mixtures preparations hydroxylase, which are the two enzymes essential for collagen biosynthesis (Pinnel, 1985). As such in our study, vitamin C was used as a positive control as well as vitamin treatment to the cells. CA with the a ...
... The effect of Centella asiatica, vitamins, glycolic acid and their mixtures preparations hydroxylase, which are the two enzymes essential for collagen biosynthesis (Pinnel, 1985). As such in our study, vitamin C was used as a positive control as well as vitamin treatment to the cells. CA with the a ...
Effect of the turbot aquareovirus on fish macrophages using an in
... (Lupiani et al. 1989) we have focused on the characterization of this virus under a wide range of circumstances. One topic studied was the route of entry of the viral agent into the host and its dissemination throughout the fish organs and tissues. In the course of such studies it has been surprisin ...
... (Lupiani et al. 1989) we have focused on the characterization of this virus under a wide range of circumstances. One topic studied was the route of entry of the viral agent into the host and its dissemination throughout the fish organs and tissues. In the course of such studies it has been surprisin ...
PDF
... impregnated with media which had supported the growth of myoblasts or notochord cells. This constituted a slow-release, unilateral source of any substances released by these cells (see below). Culture medium was plated into the other half of the chamber and contacted the agar slab. One hour later, m ...
... impregnated with media which had supported the growth of myoblasts or notochord cells. This constituted a slow-release, unilateral source of any substances released by these cells (see below). Culture medium was plated into the other half of the chamber and contacted the agar slab. One hour later, m ...
Hopanoids Play a Role in Membrane Integrity and pH
... noted, FW medium was supplemented from sterile anoxic stock solutions with 2 mM sodium acetate for photoheterotrophic growth and 2 mM sodium thiosulfate for photoautotrophic growth. All anaerobic cultures were flushed and pressurized to 5 lb/in2 with N2/CO2 (80%/20%) and incubated at 30°C at 50 W/m2 ...
... noted, FW medium was supplemented from sterile anoxic stock solutions with 2 mM sodium acetate for photoheterotrophic growth and 2 mM sodium thiosulfate for photoautotrophic growth. All anaerobic cultures were flushed and pressurized to 5 lb/in2 with N2/CO2 (80%/20%) and incubated at 30°C at 50 W/m2 ...
Microtubule
... switch between two states. (Figure 2A) The switch from growth to shrinkage is called a catastrophe, and the switch from shrinkage to growth is called a rescue. Dynamic instability is due to the structural differences between the growing and the shrinking ends. If the nucleotide hydrolysis proceeds m ...
... switch between two states. (Figure 2A) The switch from growth to shrinkage is called a catastrophe, and the switch from shrinkage to growth is called a rescue. Dynamic instability is due to the structural differences between the growing and the shrinking ends. If the nucleotide hydrolysis proceeds m ...
Systematic identification of cell size regulators in
... START only when its size exceeds some threshold (Johnston et al, 1979). The real picture is, however, more complex. The compensation for size fluctuations is only partial, so that the size at START is not fixed but depends on cell size at birth and on its volume growth rate (Johnston et al, 1979; Fe ...
... START only when its size exceeds some threshold (Johnston et al, 1979). The real picture is, however, more complex. The compensation for size fluctuations is only partial, so that the size at START is not fixed but depends on cell size at birth and on its volume growth rate (Johnston et al, 1979; Fe ...
Extensive tissue-specific transcriptomic plasticity in maize primary
... into specialized zones of development, including from the terminal end the root cap, the apical meristem, the cell elongation zone, and the maturation zone (Ishikawa and Evans, 1995). The root cap covers and thus protects the root tip, secretes mucilage to facilitate root movement, and acts as a sen ...
... into specialized zones of development, including from the terminal end the root cap, the apical meristem, the cell elongation zone, and the maturation zone (Ishikawa and Evans, 1995). The root cap covers and thus protects the root tip, secretes mucilage to facilitate root movement, and acts as a sen ...
Pausing of Golgi Bodies on Microtubules Regulates
... intracellular compartments (Figure 3E) identical in appearance, behavior, and velocity to those observed in untreated basal hypocotyl cells and those induced by osmotic stress or cycloheximide treatment (cf. Figures 2D, 3A, 3B, and 3E; see Supplemental Figure 2G online). Mock treatment with an ident ...
... intracellular compartments (Figure 3E) identical in appearance, behavior, and velocity to those observed in untreated basal hypocotyl cells and those induced by osmotic stress or cycloheximide treatment (cf. Figures 2D, 3A, 3B, and 3E; see Supplemental Figure 2G online). Mock treatment with an ident ...
Full Text - International Journal of Livestock Research
... Fabricius, the primary site for B-lymphopoiesis which is responsible for humoral immunity (HI) and the ...
... Fabricius, the primary site for B-lymphopoiesis which is responsible for humoral immunity (HI) and the ...
Free Article - Crop Functional Genomics
... into specialized zones of development, including from the terminal end the root cap, the apical meristem, the cell elongation zone, and the maturation zone (Ishikawa and Evans, 1995). The root cap covers and thus protects the root tip, secretes mucilage to facilitate root movement, and acts as a sen ...
... into specialized zones of development, including from the terminal end the root cap, the apical meristem, the cell elongation zone, and the maturation zone (Ishikawa and Evans, 1995). The root cap covers and thus protects the root tip, secretes mucilage to facilitate root movement, and acts as a sen ...
Pausing of Golgi Bodies on Microtubules Regulates
... intracellular compartments (Figure 3E) identical in appearance, behavior, and velocity to those observed in untreated basal hypocotyl cells and those induced by osmotic stress or cycloheximide treatment (cf. Figures 2D, 3A, 3B, and 3E; see Supplemental Figure 2G online). Mock treatment with an ident ...
... intracellular compartments (Figure 3E) identical in appearance, behavior, and velocity to those observed in untreated basal hypocotyl cells and those induced by osmotic stress or cycloheximide treatment (cf. Figures 2D, 3A, 3B, and 3E; see Supplemental Figure 2G online). Mock treatment with an ident ...
Divergent mechanisms underlie Smad4
... Background: Functional inactivation of the tumor suppressor Smad4 in colorectal and pancreatic carcinogenesis occurs coincident with the transition to invasive growth. Breaking the basement membrane (BM) barrier, a prerequisite for invasive growth, can be due to tumor induced proteolytic tissue remo ...
... Background: Functional inactivation of the tumor suppressor Smad4 in colorectal and pancreatic carcinogenesis occurs coincident with the transition to invasive growth. Breaking the basement membrane (BM) barrier, a prerequisite for invasive growth, can be due to tumor induced proteolytic tissue remo ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.