GATA-2 functions downstream of BMPs and CaM KIV in ectodermal
... Multicellular organisms begin with a fertilized egg (zygote), which gives rise to many different cell types that form the organism. The allocation of different cell-types into specific tissues and organs is governed by a mechanism called specification and differentiation. During the development of t ...
... Multicellular organisms begin with a fertilized egg (zygote), which gives rise to many different cell types that form the organism. The allocation of different cell-types into specific tissues and organs is governed by a mechanism called specification and differentiation. During the development of t ...
Sox3 - Prodinra
... first EMT event occurs at gastrulation, when a subset of initial epiblast cells moves to the primitive streak, delaminates and generates the mesoderm and the endoderm. Cells that remain in the epiblast keep their epithelial character and will contribute to the ectodermal derivatives, the epidermis, ...
... first EMT event occurs at gastrulation, when a subset of initial epiblast cells moves to the primitive streak, delaminates and generates the mesoderm and the endoderm. Cells that remain in the epiblast keep their epithelial character and will contribute to the ectodermal derivatives, the epidermis, ...
Cross-Presentation Proteases in MHC Class I Presentation and
... case; however, caution is warranted in interpreting these results because, at the concentrations used in these studies, most proteasome inhibitors do not block all of the proteasome’s active sites (18). Therefore, in the presence of these inhibitors, proteasomes may still be making cleavages that ge ...
... case; however, caution is warranted in interpreting these results because, at the concentrations used in these studies, most proteasome inhibitors do not block all of the proteasome’s active sites (18). Therefore, in the presence of these inhibitors, proteasomes may still be making cleavages that ge ...
- RichardWheeler.net
... 2.8.1 Important considerations in the preparation of cells for SBFSEM........ 530 2.8.2 Primary fixation of cells from a suspension culture ............................ 532 2.8.3 Postfixation, dehydration, and resin embedding................................. 532 2.8.4 Preparation of blocks for SBFSE ...
... 2.8.1 Important considerations in the preparation of cells for SBFSEM........ 530 2.8.2 Primary fixation of cells from a suspension culture ............................ 532 2.8.3 Postfixation, dehydration, and resin embedding................................. 532 2.8.4 Preparation of blocks for SBFSE ...
AMPK regulates ER morphology and function in
... mouse islets and clonal -cells We employed several imaging techniques to study ER morphology in ER stress. Primary mouse -cells were infected with a baculovirus expressing ER-Gfp and then treated with palmitate or the SERCA inhibitor thapsigargin for different periods of time followed by confocal ...
... mouse islets and clonal -cells We employed several imaging techniques to study ER morphology in ER stress. Primary mouse -cells were infected with a baculovirus expressing ER-Gfp and then treated with palmitate or the SERCA inhibitor thapsigargin for different periods of time followed by confocal ...
Antisickling Activity and Membrane Stabilizing Effect of
... NaCl by increasing the volume of the RBCs, reverting the sickling to produce biconcave cells, and thereby, maintaining membrane integrity. The sickling modifies the membrane flexibility, which would make it more fragile and would increase the precocious risk of hemolysis. But it is as possible as th ...
... NaCl by increasing the volume of the RBCs, reverting the sickling to produce biconcave cells, and thereby, maintaining membrane integrity. The sickling modifies the membrane flexibility, which would make it more fragile and would increase the precocious risk of hemolysis. But it is as possible as th ...
Gelatin fragments block adherence of Candida
... was apparent from results obtained in adherence experiments using the crude digests of gelatin obtained from digestion with trypsin and CNBr. Furthermore, when CNBr digests were further separated by column chromatography with Sephadex G-100 into peptides of differing molecular mass it was apparent t ...
... was apparent from results obtained in adherence experiments using the crude digests of gelatin obtained from digestion with trypsin and CNBr. Furthermore, when CNBr digests were further separated by column chromatography with Sephadex G-100 into peptides of differing molecular mass it was apparent t ...
Liver development
... factors such as Foxa2, Gata4–6 and Hhex, which have important roles in early foregut organogenesis. In vivo DNAbinding studies in the mouse embryonic endoderm suggest that Foxa and Gata factors bind to enhancer elements of the Albumin gene to enhance chromatin accessibility and increase the ability ...
... factors such as Foxa2, Gata4–6 and Hhex, which have important roles in early foregut organogenesis. In vivo DNAbinding studies in the mouse embryonic endoderm suggest that Foxa and Gata factors bind to enhancer elements of the Albumin gene to enhance chromatin accessibility and increase the ability ...
Lysosomes and Fas-mediated liver cell death
... The involvement of lysosomes in apoptosis as suggested by several studies is still highly debatable (for a review, see [1]). Lysosome-mediated apoptosis would result from a destabilization of the lysosomal membrane causing a release of cathepsins into the cytosol. These proteases would stimulate the ...
... The involvement of lysosomes in apoptosis as suggested by several studies is still highly debatable (for a review, see [1]). Lysosome-mediated apoptosis would result from a destabilization of the lysosomal membrane causing a release of cathepsins into the cytosol. These proteases would stimulate the ...
RCAI Annual Report 2006 (PDF 5.6M)
... rather than competing with the universities, can complement the university research system. What is most significant with RCAI is the focus on young investigators at the beginning of their career. Well over two-thirds of the research laboratories at the Center are lead by young investigators, many o ...
... rather than competing with the universities, can complement the university research system. What is most significant with RCAI is the focus on young investigators at the beginning of their career. Well over two-thirds of the research laboratories at the Center are lead by young investigators, many o ...
Plastid degeneration in Tillandsia (Bromeliaceae) provides
... flowers. These stacks consisted of about 5-10 double membranes, situated at the periphery of the organelle (Al-Babili et al., 1999). In chromoplasts of Capsicum annuum fruit single circular thylakoid sheets were observed as well as configurations of sheets which were either linear or rolled-up (Spur ...
... flowers. These stacks consisted of about 5-10 double membranes, situated at the periphery of the organelle (Al-Babili et al., 1999). In chromoplasts of Capsicum annuum fruit single circular thylakoid sheets were observed as well as configurations of sheets which were either linear or rolled-up (Spur ...
NuMA assembles into an extensive filamentous structure when
... the localization of NuMA in the nucleus establishes an inherent asymmetry to its distribution which is necessary for its role in directing the microtubules of the developing mitotic spindle toward the chromosomes during assembly of the mitotic spindle (Compton and Luo, 1995). Given the strict locali ...
... the localization of NuMA in the nucleus establishes an inherent asymmetry to its distribution which is necessary for its role in directing the microtubules of the developing mitotic spindle toward the chromosomes during assembly of the mitotic spindle (Compton and Luo, 1995). Given the strict locali ...
Cyclic AMP-Mediated Inhibition of Cell Growth Requires the Small G
... Cell culturing conditions and treatments. Hek293 and NIH 3T3 cells were cultured in Dulbecco modified Eagle medium (DMEM) plus 10% fetal calf serum at 37°C in 5% CO2. Cells were maintained in serum-free DMEM for 16 h at 37°C in 5% CO2 prior to treatment with various reagents for coimmunoprecipitatio ...
... Cell culturing conditions and treatments. Hek293 and NIH 3T3 cells were cultured in Dulbecco modified Eagle medium (DMEM) plus 10% fetal calf serum at 37°C in 5% CO2. Cells were maintained in serum-free DMEM for 16 h at 37°C in 5% CO2 prior to treatment with various reagents for coimmunoprecipitatio ...
Risk Reduction in Drinking Water Distribution Systems by On
... Studies involving the blue-fluorescent strain Legionella bozemanii were initiated in the fourth quarter of this year (Objective 6). Biofilm formation was monitored on-line using the bacterial autofluorescence which was more sensitive than tryptophan fluorescence. Preliminary results demonstrated tha ...
... Studies involving the blue-fluorescent strain Legionella bozemanii were initiated in the fourth quarter of this year (Objective 6). Biofilm formation was monitored on-line using the bacterial autofluorescence which was more sensitive than tryptophan fluorescence. Preliminary results demonstrated tha ...
Oct-4 controls cell-cycle progression of embryonic stem cells
... derived ES cell lines used in the present study (Figure 2B, lanes 2–5). Consistent with previous reports [12,24,25], the Oct-4transfected ZHBTc4 ES cells were able to maintain their selfrenewal ability and stem-cell phenotype (Figure 2C, second panels). As a control, ZHBTc4 ES cells stably transfect ...
... derived ES cell lines used in the present study (Figure 2B, lanes 2–5). Consistent with previous reports [12,24,25], the Oct-4transfected ZHBTc4 ES cells were able to maintain their selfrenewal ability and stem-cell phenotype (Figure 2C, second panels). As a control, ZHBTc4 ES cells stably transfect ...
267 CHAPTER 6 Discussion of Results The aim of
... transplantation and ongoing epidermis of human immune deficiency virus (HIV) infections. This situation provided the momentum to the search for new antimicrobial substances from different source like medicinal plants. The use of plant extracts with known antimicrobial properties can be of vast signi ...
... transplantation and ongoing epidermis of human immune deficiency virus (HIV) infections. This situation provided the momentum to the search for new antimicrobial substances from different source like medicinal plants. The use of plant extracts with known antimicrobial properties can be of vast signi ...
Cloning, Expression, and Functional Characterization of TL1A-Ig
... 1.1 The immune system: innate and adaptive arms The immune system consists of molecules and cells that work together to protect the individual from microbial infection and maintain homeostasis. The immune system is comprised of the innate and adaptive immune responses. Together these responses fulfi ...
... 1.1 The immune system: innate and adaptive arms The immune system consists of molecules and cells that work together to protect the individual from microbial infection and maintain homeostasis. The immune system is comprised of the innate and adaptive immune responses. Together these responses fulfi ...
Journal of Bacteriology
... nitrogen-fixing root nodules. The physical interaction between rhizobia and the growing root hair tips precedes the following infection stages in the nodulation process: marked root hair curling, infection thread formation, development of a root cortical meristem, and root nodule morphogenesis (7). ...
... nitrogen-fixing root nodules. The physical interaction between rhizobia and the growing root hair tips precedes the following infection stages in the nodulation process: marked root hair curling, infection thread formation, development of a root cortical meristem, and root nodule morphogenesis (7). ...
Steel Factor Induces Serine Phosphorylation of
... Tyrosine phosphorylation of Stat family proteins in factordependent cell lines. Tyrosine phosphorylation of Stat family proteins, presumably by Jak family tyrosine kinases, has been shown to be essential for their DNA binding and transcriptional activation.' We first examined the effect of various h ...
... Tyrosine phosphorylation of Stat family proteins in factordependent cell lines. Tyrosine phosphorylation of Stat family proteins, presumably by Jak family tyrosine kinases, has been shown to be essential for their DNA binding and transcriptional activation.' We first examined the effect of various h ...
Dynamics of the slowing segmentation clock reveal
... the basis of most somitogenesis models today (Lewis et al., 2009; Aulehla and Pourquié, 2010; Pourquié, 2011; Oates et al., 2012). Through experimental and theoretical data across a wide breadth of organisms, the periodicity and timing of somite formation is now thought to be controlled by a molecul ...
... the basis of most somitogenesis models today (Lewis et al., 2009; Aulehla and Pourquié, 2010; Pourquié, 2011; Oates et al., 2012). Through experimental and theoretical data across a wide breadth of organisms, the periodicity and timing of somite formation is now thought to be controlled by a molecul ...
Cell Fate Specification in the C. elegans Embryo
... and tissues. When a cell has been specified for a particular fate, it is capable of generating those tissues if cultured away from the remainder of the embryo (Wolpert et al., 2007). As all embryonic cells are derived from the zygote by mitosis, cells must acquire differences in gene expression over ...
... and tissues. When a cell has been specified for a particular fate, it is capable of generating those tissues if cultured away from the remainder of the embryo (Wolpert et al., 2007). As all embryonic cells are derived from the zygote by mitosis, cells must acquire differences in gene expression over ...
WRL3824.tmp
... 88. A lot of biochemical functions of water soluble vitamins are related to their ability to transform in cells in the proper coenzymes. Which from the below mentioned vitamins is needed for formation of FMN and FAD? A. Vitamin B6 B. Vitamin B1 C. Vitamin B5 D. Vitamin B3 E. * Vitamin B2 89. A poor ...
... 88. A lot of biochemical functions of water soluble vitamins are related to their ability to transform in cells in the proper coenzymes. Which from the below mentioned vitamins is needed for formation of FMN and FAD? A. Vitamin B6 B. Vitamin B1 C. Vitamin B5 D. Vitamin B3 E. * Vitamin B2 89. A poor ...
Journal of Cell Science • Advance article Rop, the
... and reside at the embryo surface, furrow formation, ingression, and abscission are easily imaged live. Such live-imaging reveals what stage cytokinesis fails at and detects phenotypes more subtle than failure, which are missed by a single time-point fixed analysis. Importantly, vesicle delivery to t ...
... and reside at the embryo surface, furrow formation, ingression, and abscission are easily imaged live. Such live-imaging reveals what stage cytokinesis fails at and detects phenotypes more subtle than failure, which are missed by a single time-point fixed analysis. Importantly, vesicle delivery to t ...
Ccbe1 regulates Vegfc-mediated induction of Vegfr3
... from vegfr3 and ccbe1 mutants at the level of gross embryo morphology (supplementary material Fig. S1A-D), and that displayed a severe reduction in secondary angiogenesis and block in lymphatic precursor sprouting from the cardinal vein (Fig. 1A-D; Fig. 2A-H; supplementary material Fig. S1E-K). At 5 ...
... from vegfr3 and ccbe1 mutants at the level of gross embryo morphology (supplementary material Fig. S1A-D), and that displayed a severe reduction in secondary angiogenesis and block in lymphatic precursor sprouting from the cardinal vein (Fig. 1A-D; Fig. 2A-H; supplementary material Fig. S1E-K). At 5 ...
Cardioprotective Effects of Erythropoietin in Rats Subjected to
... injury. In this study, we assessed the effect of EPO on 99mTcannexin V myocardial uptake and whether the accumulation of 99mTc-annexin V can predict cardiac remodeling and functional deterioration. Methods: Eighteen rats with left coronary artery (LCA) occlusion were randomized to receive either an ...
... injury. In this study, we assessed the effect of EPO on 99mTcannexin V myocardial uptake and whether the accumulation of 99mTc-annexin V can predict cardiac remodeling and functional deterioration. Methods: Eighteen rats with left coronary artery (LCA) occlusion were randomized to receive either an ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.