Inhibition of TCR Signaling by Herpes Simplex Virus
... gH-complemented cell line F6 (25). The HSV-1 (KOS) mutants deleted for gB (K082), originally obtained from N. A. DeLuca (University of Pittsburgh School of Medicine, Pittsburgh, PA) (28), gL (gL86) (29), and gC ⌬C2–3 (30) were provided by P. G. Spear (Northwestern University, Chicago, IL). Complemen ...
... gH-complemented cell line F6 (25). The HSV-1 (KOS) mutants deleted for gB (K082), originally obtained from N. A. DeLuca (University of Pittsburgh School of Medicine, Pittsburgh, PA) (28), gL (gL86) (29), and gC ⌬C2–3 (30) were provided by P. G. Spear (Northwestern University, Chicago, IL). Complemen ...
Plant Molecular Biology
... 1 and 2B). A very low percentage (less than 2%) of structure-less ghosts and cellular debris that are typical for necrotic cell death were also observed after 24 h DEX treatment (data not shown). However, even after 36 or 48 h treatment, the majority of cells belonged to categories x, y and z (Figur ...
... 1 and 2B). A very low percentage (less than 2%) of structure-less ghosts and cellular debris that are typical for necrotic cell death were also observed after 24 h DEX treatment (data not shown). However, even after 36 or 48 h treatment, the majority of cells belonged to categories x, y and z (Figur ...
POP-1 and Anterior–Posterior Fate Decisions in
... Despite the different molecular mechanisms that establish blastomere identities and the very different lineages that blastomeres execute, there is suggestive evidence that cells in all parts of the embryo share a mechanism for recognizing the division sequences by which they were born. Several mutan ...
... Despite the different molecular mechanisms that establish blastomere identities and the very different lineages that blastomeres execute, there is suggestive evidence that cells in all parts of the embryo share a mechanism for recognizing the division sequences by which they were born. Several mutan ...
Intracellular catalysis of disulfide bond formation by the human
... has been identified in human and avian QSOX sequences [8]. Thus, while the ER oxidation system requires interaction between Ero1/Erv2p and PDI, the QSOX enzymes have evolved by a fusion of these two proteins, providing them with the unique advantage of being able to introduce disulfide bonds into su ...
... has been identified in human and avian QSOX sequences [8]. Thus, while the ER oxidation system requires interaction between Ero1/Erv2p and PDI, the QSOX enzymes have evolved by a fusion of these two proteins, providing them with the unique advantage of being able to introduce disulfide bonds into su ...
PDF
... timed experiments. Identified cells in 285 cleavagestage embryos (stages l-6c) were examined; the lengths and compositions of their cell cycles are presented in Figs 3 and 4. Limitations to the accuracy of our data are as follows: (1) Embryos were fixed at 30min intervals; thus, if homologous cells ...
... timed experiments. Identified cells in 285 cleavagestage embryos (stages l-6c) were examined; the lengths and compositions of their cell cycles are presented in Figs 3 and 4. Limitations to the accuracy of our data are as follows: (1) Embryos were fixed at 30min intervals; thus, if homologous cells ...
Full text in pdf format
... in situ temperature (Zimmermann et al. 1978). Incubation was stopped by the addition of buffered formalin Bacterial abundance and biomass were highest at (final conc.: 2 O/O w/v), and bacterial cells containing red formazan crystals (400 to 600 cells) were quantified on the beginning and towards the ...
... in situ temperature (Zimmermann et al. 1978). Incubation was stopped by the addition of buffered formalin Bacterial abundance and biomass were highest at (final conc.: 2 O/O w/v), and bacterial cells containing red formazan crystals (400 to 600 cells) were quantified on the beginning and towards the ...
Optimization of ERK Activity Biosensors for both Ratiometric and
... phosphorylates and activates a third kinase (MAPK). The MAPK/ERK cascade successively enrolls MOS or Raf, MEK1/2, Extracellular Regulated Kinase (ERK) 1/2 that activates by phosphorylation a plethora of substrates within the cytoplasm and the nucleus, as effectors involved in the physiological respo ...
... phosphorylates and activates a third kinase (MAPK). The MAPK/ERK cascade successively enrolls MOS or Raf, MEK1/2, Extracellular Regulated Kinase (ERK) 1/2 that activates by phosphorylation a plethora of substrates within the cytoplasm and the nucleus, as effectors involved in the physiological respo ...
Antigen Export during Liver Infection of the Malaria - edoc
... which are presented by DCs in the priming phase and by infected hepatocytes to CD8⫹ effector T cells, which in turn eliminate liver-stage parasites (8, 23, 24). A recent study showed that presentation of CSP that contained the very potent H-2Kd ovalbumin (OVA) epitope to CD8⫹ T cells occurs by the t ...
... which are presented by DCs in the priming phase and by infected hepatocytes to CD8⫹ effector T cells, which in turn eliminate liver-stage parasites (8, 23, 24). A recent study showed that presentation of CSP that contained the very potent H-2Kd ovalbumin (OVA) epitope to CD8⫹ T cells occurs by the t ...
Arabidopsis RHD3 mediates the generation of the tubular ER
... the lumen of the ER at the C-terminus (Hu et al., 2009). It has been hypothesized that the atlastins and Sey1p might be required in the generation of local ER membrane curvatures that are necessary for either fission or fusion of ER tubules (Hu et al., 2009). In humans there are three atlastin isofo ...
... the lumen of the ER at the C-terminus (Hu et al., 2009). It has been hypothesized that the atlastins and Sey1p might be required in the generation of local ER membrane curvatures that are necessary for either fission or fusion of ER tubules (Hu et al., 2009). In humans there are three atlastin isofo ...
MyRIP, a novel Rab effector, enables myosin VIIa recruitment to
... melanosomes (Figure 3C and D). Myosin VIIa (Figure 3E; Liu et al., 1998) and Rab27A (Figure 3F) were also associated with retinal melanosomes. Since MyRIP was present also in the synaptic areas of retinal photoreceptor cells (Figure 4A) and inner ear hair cells, we used the PC12 neuroendocrine cell ...
... melanosomes (Figure 3C and D). Myosin VIIa (Figure 3E; Liu et al., 1998) and Rab27A (Figure 3F) were also associated with retinal melanosomes. Since MyRIP was present also in the synaptic areas of retinal photoreceptor cells (Figure 4A) and inner ear hair cells, we used the PC12 neuroendocrine cell ...
View Full Page PDF - The American Biology Teacher
... 1891). These cells (see Glossary) both replenish themselves and differentiate anew into tissue-specific cells that replace those lost to physiological wear and tear and/or injury (Smith et al., 1991). The presence and proliferation of highly potent stem cells in both vertebrates and invertebrates ar ...
... 1891). These cells (see Glossary) both replenish themselves and differentiate anew into tissue-specific cells that replace those lost to physiological wear and tear and/or injury (Smith et al., 1991). The presence and proliferation of highly potent stem cells in both vertebrates and invertebrates ar ...
Retinoic acid-mediated activation of HNF-3a
... Xenopus proteins are clearly involved in early developmental decision processes, confirming that members of the HNF-3 family play a critical role in early development and are not restricted to the regulation of tissue-specific gene expression [31]. In this article, we have focused on the development ...
... Xenopus proteins are clearly involved in early developmental decision processes, confirming that members of the HNF-3 family play a critical role in early development and are not restricted to the regulation of tissue-specific gene expression [31]. In this article, we have focused on the development ...
Nitric Oxide 9:
... [9,16], which becomes activated in cells exposed to H2 O2 or nitric oxide (NO) via oxidation or nitrosation, respectively, of an OxyR cysteine redox center [9,17–19]. H2 O2 and NO can also activate SoxR, another transcriptional global regulator of genes involved in oxidative stress tolerance, such a ...
... [9,16], which becomes activated in cells exposed to H2 O2 or nitric oxide (NO) via oxidation or nitrosation, respectively, of an OxyR cysteine redox center [9,17–19]. H2 O2 and NO can also activate SoxR, another transcriptional global regulator of genes involved in oxidative stress tolerance, such a ...
Virus entry into a polarized epithelial cell line (MDCK)
... fluid was harvested after incubation of the eggs for 3 days at 33 °C (Herrler & Klenk, 1987). This virus was stored at - 8 0 °C and used to infect MDCK cells. Strain L-9 of BCV was grown in MDCK I cells as described previously (Schultze et al., 1990). The kinetics of virus growth in MDCK I cells was ...
... fluid was harvested after incubation of the eggs for 3 days at 33 °C (Herrler & Klenk, 1987). This virus was stored at - 8 0 °C and used to infect MDCK cells. Strain L-9 of BCV was grown in MDCK I cells as described previously (Schultze et al., 1990). The kinetics of virus growth in MDCK I cells was ...
Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in
... catalyzing the committed step: the transfer of a fatty acyl moiety from acyl-CoA to the sn-3 position of diacylglycerol (Kennedy, 1961). As such, DGAT plays an essential role in controlling both the quantitative (Ichihara et al., 1988) and qualitative (Vogel and Browse, 1996; He et al., 2004a) flux ...
... catalyzing the committed step: the transfer of a fatty acyl moiety from acyl-CoA to the sn-3 position of diacylglycerol (Kennedy, 1961). As such, DGAT plays an essential role in controlling both the quantitative (Ichihara et al., 1988) and qualitative (Vogel and Browse, 1996; He et al., 2004a) flux ...
autophagy - Botanik in Bonn
... proteins and organelles such as the endoplasmic reticulum, mitochondria, peroxisomes, the nucleus and ribosomes [2,3] ...
... proteins and organelles such as the endoplasmic reticulum, mitochondria, peroxisomes, the nucleus and ribosomes [2,3] ...
Virus entry into a polarized epithelial cell line (MDCK)
... fluid was harvested after incubation of the eggs for 3 days at 33 °C (Herrler & Klenk, 1987). This virus was stored at - 8 0 °C and used to infect MDCK cells. Strain L-9 of BCV was grown in MDCK I cells as described previously (Schultze et al., 1990). The kinetics of virus growth in MDCK I cells was ...
... fluid was harvested after incubation of the eggs for 3 days at 33 °C (Herrler & Klenk, 1987). This virus was stored at - 8 0 °C and used to infect MDCK cells. Strain L-9 of BCV was grown in MDCK I cells as described previously (Schultze et al., 1990). The kinetics of virus growth in MDCK I cells was ...
Reversal of Physiological Stress
... Solid tumors often have irregular and inadequate vascularization because of the uncontrolled cellular growth associated with tumor formation. Inadequate blood flow creates cell subpopulations within tumors that are hypoxic and/or glucose-deprived (Vaupel et al., 1989). These physiological stress con ...
... Solid tumors often have irregular and inadequate vascularization because of the uncontrolled cellular growth associated with tumor formation. Inadequate blood flow creates cell subpopulations within tumors that are hypoxic and/or glucose-deprived (Vaupel et al., 1989). These physiological stress con ...
PDF with detailed project information
... binding. Importantly, our experimental approach is suitable not only for endogenous small molecules, but also for the identification of protein partners of exogenous compounds, such as drugs and herbicides. At present, the small molecule signalling group (established in February 2015) comprises two ...
... binding. Importantly, our experimental approach is suitable not only for endogenous small molecules, but also for the identification of protein partners of exogenous compounds, such as drugs and herbicides. At present, the small molecule signalling group (established in February 2015) comprises two ...
Circuits that build visual cortical receptive fields
... subfields [19,20]. This practice sometimes expanded to include all cells that had just one subregion, such that many cells are called complex (or C1) by some [2,18–23] and simple by others (e.g. [16,24]). Furthermore, many investigators classify simple and complex cells by response properties such a ...
... subfields [19,20]. This practice sometimes expanded to include all cells that had just one subregion, such that many cells are called complex (or C1) by some [2,18–23] and simple by others (e.g. [16,24]). Furthermore, many investigators classify simple and complex cells by response properties such a ...
7-2 Eukaryotic Cell Structure
... Lysosomes are small organelles filled with enzymes. Lysosomes break down lipids, carbohydrates, and proteins into small molecules that can be used by the rest of the cell. Lysosomes also break down organelles that have outlived their usefulness. Slide 23 of 49 Copyright Pearson Prentice Hall ...
... Lysosomes are small organelles filled with enzymes. Lysosomes break down lipids, carbohydrates, and proteins into small molecules that can be used by the rest of the cell. Lysosomes also break down organelles that have outlived their usefulness. Slide 23 of 49 Copyright Pearson Prentice Hall ...
Islet Amyloid Polypeptide Is a Target Antigen for
... mass spectrometric analysis of peak antigenic fractions. Similar to the other clones from the panel, BDC-5.2.9 responds in vitro to pancreatic islet cells or cell extracts of b-cell adenomas presented in the context of I-Ag7 (2). Our starting source of antigen was b-cell tumor tissue excised from NO ...
... mass spectrometric analysis of peak antigenic fractions. Similar to the other clones from the panel, BDC-5.2.9 responds in vitro to pancreatic islet cells or cell extracts of b-cell adenomas presented in the context of I-Ag7 (2). Our starting source of antigen was b-cell tumor tissue excised from NO ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.