Identification of a novel population of muscle stem cells in mice
... centages of desmin-expressing cells were still observed between the populations. Unlike EP cells, most MDSC (60%) do not express the myogenic marker desmin, and a small population (10%) of these cells is Sca-1/CD34; this latter characteristic also is found in primary hematopoietic stem cells (Nak ...
... centages of desmin-expressing cells were still observed between the populations. Unlike EP cells, most MDSC (60%) do not express the myogenic marker desmin, and a small population (10%) of these cells is Sca-1/CD34; this latter characteristic also is found in primary hematopoietic stem cells (Nak ...
PAMAM Dendrimer Conjugates for Intracellular Delivery of N
... overdose cases. The determination of its antioxidant, radical-scavenging properties, gave rise to investigation of its other therapeutic applications. Even though NAC proved to be a very therapeutic drug, delivery problems remain. The stability of NAC is low which increases the effective dose requir ...
... overdose cases. The determination of its antioxidant, radical-scavenging properties, gave rise to investigation of its other therapeutic applications. Even though NAC proved to be a very therapeutic drug, delivery problems remain. The stability of NAC is low which increases the effective dose requir ...
OCTOPUS, a polarly localised membrane
... developing vasculature (Scarpella et al., 2006). However, not all vascular patterning mutants show altered auxin transport or response, indicating that auxin-independent factors also play a role in this process (Candela et al., 1999; Carland et al., 1999; Carland et al., 2002). Notably, vascular dev ...
... developing vasculature (Scarpella et al., 2006). However, not all vascular patterning mutants show altered auxin transport or response, indicating that auxin-independent factors also play a role in this process (Candela et al., 1999; Carland et al., 1999; Carland et al., 2002). Notably, vascular dev ...
silicone rubber in a parallel plate flow chamber in the absence and
... compared adhesion of Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli to orthopaedic implant polymers for organisms suspended in phosphate-buffered saline (PBS), tryptic soy broth and hyaluronic acid. They found that adhesion in whole broth was decreased compared to adhesion i ...
... compared adhesion of Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli to orthopaedic implant polymers for organisms suspended in phosphate-buffered saline (PBS), tryptic soy broth and hyaluronic acid. They found that adhesion in whole broth was decreased compared to adhesion i ...
silicone rubber in a parallel plate flow chamber in the
... compared adhesion of Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli to orthopaedic implant polymers for organisms suspended in phosphate-buffered saline (PBS), tryptic soy broth and hyaluronic acid. They found that adhesion in whole broth was decreased compared to adhesion i ...
... compared adhesion of Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli to orthopaedic implant polymers for organisms suspended in phosphate-buffered saline (PBS), tryptic soy broth and hyaluronic acid. They found that adhesion in whole broth was decreased compared to adhesion i ...
Calcium: a regulation system emerges in plant cells
... calcium to between 10~6 and 10~5 M that may follow plasma membrane perturbation and alteration of calcium channel activity, as signals, eliciting a variety of predetermined responses. The concentration of cytoplasmic calcium is sensed by calcium-binding proteins, most notably calmodulin, and the cal ...
... calcium to between 10~6 and 10~5 M that may follow plasma membrane perturbation and alteration of calcium channel activity, as signals, eliciting a variety of predetermined responses. The concentration of cytoplasmic calcium is sensed by calcium-binding proteins, most notably calmodulin, and the cal ...
Differential Expression Control and Polarized Distribution of Plasma
... cell (Fig. 1F, H, I). Although in non-dividing cells the fluorescence of GFP–SYP132 was only detected on the PM, in dividing cells its fluorescence was also observed in cytosolic punctate structures in addition to the cell plates (asterisks in Fig. 1F). It has been reported elsewhere that PM-localized ...
... cell (Fig. 1F, H, I). Although in non-dividing cells the fluorescence of GFP–SYP132 was only detected on the PM, in dividing cells its fluorescence was also observed in cytosolic punctate structures in addition to the cell plates (asterisks in Fig. 1F). It has been reported elsewhere that PM-localized ...
Nitrogen gas flushing can be bactericidal: the temperature
... milk (Huck et al., 2009; Ivy et al., 2012). B. weihenstephanensis was earlier distinguished from B. cereus by its ability to grow at 7 and not at 43◦ C (Lechner et al., 1998; Von Stetten et al., 1999) observed that B. weihenstephanensis was still able to grow at 38◦ C. The species comprises most of ...
... milk (Huck et al., 2009; Ivy et al., 2012). B. weihenstephanensis was earlier distinguished from B. cereus by its ability to grow at 7 and not at 43◦ C (Lechner et al., 1998; Von Stetten et al., 1999) observed that B. weihenstephanensis was still able to grow at 38◦ C. The species comprises most of ...
Expression of Cell Adhesion Molecule E
... member of the cadherin gene family, is responsible for the Ca2+-dependent adhesion between cleavage stage Xenopus blastomeres. Detectable accumulation of E-cadherin started just before gastrulation at stage 91/2 and increased rapidly up to the end of gastrulation at stage 15. In stage 15 embryos, sp ...
... member of the cadherin gene family, is responsible for the Ca2+-dependent adhesion between cleavage stage Xenopus blastomeres. Detectable accumulation of E-cadherin started just before gastrulation at stage 91/2 and increased rapidly up to the end of gastrulation at stage 15. In stage 15 embryos, sp ...
Targeting Robo4-dependent slit signaling to survive the cytokine storm in sepsis and influenza. Science Trans Med 2, 1-9.
... The prominent role of cytokines in these pathologies has led to the testing of agents that reduce cytokine signaling as possible therapeutics (10). This clinical strategy has, however, been disappointing, often resulting in increased mortality (11–13). The converse strategy of boosting the immune sy ...
... The prominent role of cytokines in these pathologies has led to the testing of agents that reduce cytokine signaling as possible therapeutics (10). This clinical strategy has, however, been disappointing, often resulting in increased mortality (11–13). The converse strategy of boosting the immune sy ...
Uptake and presentation of hepatitis C virus–like
... structural proteins in insect cells and are characterized by morphologic, biophysical, and antigenic properties similar to putative virions from infected humans.27-29,32-34 In vivo, HCV-LPs induce a strong humoral and cellular immune response including HCVspecific helper T cells and cytotoxic T lymp ...
... structural proteins in insect cells and are characterized by morphologic, biophysical, and antigenic properties similar to putative virions from infected humans.27-29,32-34 In vivo, HCV-LPs induce a strong humoral and cellular immune response including HCVspecific helper T cells and cytotoxic T lymp ...
Salt-Induced Remodeling of Spatially Restricted
... Although the operation and physiological relevance of endocytosis have been demonstrated unequivocally in plants, the molecular components participating in the process are less well characterized than in animal systems. Simple, well-defined tissue organization makes the Arabidopsis thaliana root an i ...
... Although the operation and physiological relevance of endocytosis have been demonstrated unequivocally in plants, the molecular components participating in the process are less well characterized than in animal systems. Simple, well-defined tissue organization makes the Arabidopsis thaliana root an i ...
Ethanol Induces Heterotopias in Organotypic
... cells, are disorganized (Derer, 1985) and dysfunctional (D’Arcangelo et al., 1995). A small collection of lissencephaly cases in humans are linked to mutations in the reeler gene, ...
... cells, are disorganized (Derer, 1985) and dysfunctional (D’Arcangelo et al., 1995). A small collection of lissencephaly cases in humans are linked to mutations in the reeler gene, ...
Negative control of cell size in the cyanobacterium Synechococcus
... harvested by centrifugation and the pellet resuspended in 100 lL of lysis buffer: 25 mM Tris–HCl pH 8.0, 1 mM DTT, Protease inhibitor cocktail (23 mM AEBSF, 2 mM Aprotinin, 130 lM Bestatin, 100 mM EDTA, 0.3 mM E-64, 0.3 mM Pepstatin A) and PhosSTOP (Roche). Glass beads were added and the mixture hom ...
... harvested by centrifugation and the pellet resuspended in 100 lL of lysis buffer: 25 mM Tris–HCl pH 8.0, 1 mM DTT, Protease inhibitor cocktail (23 mM AEBSF, 2 mM Aprotinin, 130 lM Bestatin, 100 mM EDTA, 0.3 mM E-64, 0.3 mM Pepstatin A) and PhosSTOP (Roche). Glass beads were added and the mixture hom ...
Fungi
... by ____________ ____________, but by the processes of ________________, ___________ _______________ and ____________. Yeasts Yeasts usually reproduce asexually through a process called _____________. Budding is the _____________ of a cell to form 2 new cells, in which the new cells are always much _ ...
... by ____________ ____________, but by the processes of ________________, ___________ _______________ and ____________. Yeasts Yeasts usually reproduce asexually through a process called _____________. Budding is the _____________ of a cell to form 2 new cells, in which the new cells are always much _ ...
T Cells + Lymphotropic Virus (MTLV) Destroying CD4 Autoimmune
... To determine the role of the MTLV-induced T cell deficiency in the autoimmune development, the deficiency was sustained by removal of the thymus 3 wk after neonatal infection, and the mice were examined at 3 mo of age for the development of autoimmune disease (Table I, Fig. 5). The virus-infected an ...
... To determine the role of the MTLV-induced T cell deficiency in the autoimmune development, the deficiency was sustained by removal of the thymus 3 wk after neonatal infection, and the mice were examined at 3 mo of age for the development of autoimmune disease (Table I, Fig. 5). The virus-infected an ...
A matter of size: developmental control of organ size in plants
... (IFL1) gene suggest that the polar flow of auxin may act as a cue for organ-size control. In rev/ifl1 mutants, growth and cell proliferation persist longer than in the wildtype, resulting in larger leaves and flowers, and thicker stems [20,21]. REV/IFL1 function is required for the activity of auxin ...
... (IFL1) gene suggest that the polar flow of auxin may act as a cue for organ-size control. In rev/ifl1 mutants, growth and cell proliferation persist longer than in the wildtype, resulting in larger leaves and flowers, and thicker stems [20,21]. REV/IFL1 function is required for the activity of auxin ...
Meristem-Specific Suppression of Mitosis and a
... specific cell types their unique properties, the regulatory genes and receptors that control the process, or the signals that trigger it (Jacobs, 1994). Studies in our laboratory have confirmed an observation first documented in 1919: the sloughed root cap cells, which separate in large numbers from ...
... specific cell types their unique properties, the regulatory genes and receptors that control the process, or the signals that trigger it (Jacobs, 1994). Studies in our laboratory have confirmed an observation first documented in 1919: the sloughed root cap cells, which separate in large numbers from ...
Self-Replication Mechanism by Means of Self
... as to the capability of self-repair. Our new platform will therefore logically allow for cellular development and self-repair. The idea behind these two concepts is to let the reconfigurable part of the chip selforganize, and to potentially support fault-tolerance mechanisms. The developmental featu ...
... as to the capability of self-repair. Our new platform will therefore logically allow for cellular development and self-repair. The idea behind these two concepts is to let the reconfigurable part of the chip selforganize, and to potentially support fault-tolerance mechanisms. The developmental featu ...
A mitochondrial specific stress response in mammalian cells
... Transfection of COS-7 cells with cDNA encoding the precursor forms of wild-type or mutant OTC followed by pulse labelling and immunoprecipitation with anti-OTC antibodies showed that both were proteolytically processed by removal of the presequence in a membrane potential (Dy)-dependent manner, a pr ...
... Transfection of COS-7 cells with cDNA encoding the precursor forms of wild-type or mutant OTC followed by pulse labelling and immunoprecipitation with anti-OTC antibodies showed that both were proteolytically processed by removal of the presequence in a membrane potential (Dy)-dependent manner, a pr ...
The importance of foetal movement for co
... In the 1970s, newborns with joint contractures, pulmonary hypoplasia, facial deformities and overall growth retardation were suggested by some to suffer from specific autosomal-recessive mutations, whereas others argued that this phenotype resulted from related, though discrete, disorders.6-8 The di ...
... In the 1970s, newborns with joint contractures, pulmonary hypoplasia, facial deformities and overall growth retardation were suggested by some to suffer from specific autosomal-recessive mutations, whereas others argued that this phenotype resulted from related, though discrete, disorders.6-8 The di ...
Development of definitive endoderm from embryonic
... based on findings from studies using model systems such as Xenopus and zebrafish that provide easy access to early embryonic stages of development at a time when lineage commitment decisions are taking place. By contrast, the mouse embryo is much less amenable to such experimental approaches because ...
... based on findings from studies using model systems such as Xenopus and zebrafish that provide easy access to early embryonic stages of development at a time when lineage commitment decisions are taking place. By contrast, the mouse embryo is much less amenable to such experimental approaches because ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.