γ-Tubulin Is Essential for Microtubule Organization and
... the sides of extant microtubules, resulting in branched structures (Murata et al., 2005). They also showed that g-tubulin is required for this process. Although, in such a cell-free system many parameters (e.g., local tubulin concentrations) may differ markedly from in vivo conditions, these results ...
... the sides of extant microtubules, resulting in branched structures (Murata et al., 2005). They also showed that g-tubulin is required for this process. Although, in such a cell-free system many parameters (e.g., local tubulin concentrations) may differ markedly from in vivo conditions, these results ...
Characterization of Citrate Transport through the Plasma Membrane
... Al-tolerant maize (Pellet et al. 1995), known as one of the highest values derived from crop plants. Both Al-tolerant lines in wheat and maize are known to contain an altered organic acid transport capacity in the plasma membrane. Thus, we speculated that IPG cells could involve a similar alteration ...
... Al-tolerant maize (Pellet et al. 1995), known as one of the highest values derived from crop plants. Both Al-tolerant lines in wheat and maize are known to contain an altered organic acid transport capacity in the plasma membrane. Thus, we speculated that IPG cells could involve a similar alteration ...
Zasp is required for the assembly of functional integrin adhesion sites
... the cell edge and streaks in areas of potentially increased local forces (Fig. 1 A). In contrast, S2 cells spread on concanavalin A do not exhibit these integrin adhesion sites (Fig. 1 B). Instead of distinct foci and streaks, βPS integrin is exclusively localized intracellularly, most likely becaus ...
... the cell edge and streaks in areas of potentially increased local forces (Fig. 1 A). In contrast, S2 cells spread on concanavalin A do not exhibit these integrin adhesion sites (Fig. 1 B). Instead of distinct foci and streaks, βPS integrin is exclusively localized intracellularly, most likely becaus ...
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... (aaRSs), a family of enzymes that catalyze the ATP-dependent esterification of amino acids to the 3′ acceptor end of cognate tRNAs (Ibba and Soll, 2000). Cells generally count with a set of twenty aaRSs, each one specific for its cognate amino acid. According to structural and conservation criteria, ...
... (aaRSs), a family of enzymes that catalyze the ATP-dependent esterification of amino acids to the 3′ acceptor end of cognate tRNAs (Ibba and Soll, 2000). Cells generally count with a set of twenty aaRSs, each one specific for its cognate amino acid. According to structural and conservation criteria, ...
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... if FITC-Con A fluorescence was restricted to 7 5 % or less of the cell surface (see Reeve & Ziomek, 1980). Numbers in parentheses represent additional cells in which there was a clear heterogeneous distribution of surface stain but it was not organized into a coherent pole. % Polarized includes all ...
... if FITC-Con A fluorescence was restricted to 7 5 % or less of the cell surface (see Reeve & Ziomek, 1980). Numbers in parentheses represent additional cells in which there was a clear heterogeneous distribution of surface stain but it was not organized into a coherent pole. % Polarized includes all ...
view as pdf - KITP Online
... Advantage No3 of zebrafish as a vertebrate model organism: Preparing embryos for imaging is very easy! Using upright microscope settings to image the hindbrain ...
... Advantage No3 of zebrafish as a vertebrate model organism: Preparing embryos for imaging is very easy! Using upright microscope settings to image the hindbrain ...
When to Start Antiretroviral Treatment: A Changing Equation
... among the most vexing puzzles in the field of HIV management. HIV begins killing CD4 T-cells—key players in the body’s immune response—from the time of initial infection, yet many HIV positive people go for years without experiencing clinical symptoms. Antiretroviral drugs effectively suppress viral ...
... among the most vexing puzzles in the field of HIV management. HIV begins killing CD4 T-cells—key players in the body’s immune response—from the time of initial infection, yet many HIV positive people go for years without experiencing clinical symptoms. Antiretroviral drugs effectively suppress viral ...
The Mormyromast Region of the Mormyrid Electrosensory Lobe. I
... mo, molecular layer; peet, preeminential-electrosensory tract; pf, plexiform layer; sgr, superficial granular layer. Bars: 50 m. Both of these cells, as well as the other cells shown in subsequent figures, were recorded and studied intracellularly before being injected with biocytin for morphologic ...
... mo, molecular layer; peet, preeminential-electrosensory tract; pf, plexiform layer; sgr, superficial granular layer. Bars: 50 m. Both of these cells, as well as the other cells shown in subsequent figures, were recorded and studied intracellularly before being injected with biocytin for morphologic ...
Apical constriction: A cell shape change that can
... 2002). Internalization of the endodermal precursors has been most thoroughly studied and is the focus of our discussion here. Cell movements associated with C. elegans gastrulation can occur in vitro, allowing mechanisms to be explored by cell manipulation experiments as in sea urchins and Xenopus ( ...
... 2002). Internalization of the endodermal precursors has been most thoroughly studied and is the focus of our discussion here. Cell movements associated with C. elegans gastrulation can occur in vitro, allowing mechanisms to be explored by cell manipulation experiments as in sea urchins and Xenopus ( ...
THE PLANT CELL CYCLE Walter Dewitte and James A.H. Murray
... certain cellular aspects, including the intracellular localization of proteins, can be studied in individual cells and therefore do not require consistent timing of division between cells, the biochemical and molecular analysis of cell division is predicated on the availability of cell systems that ...
... certain cellular aspects, including the intracellular localization of proteins, can be studied in individual cells and therefore do not require consistent timing of division between cells, the biochemical and molecular analysis of cell division is predicated on the availability of cell systems that ...
A parafusin-related Toxoplasma protein in Ca -regulated secretory organelles
... Apicomplexa, but T. gondii is the most widespread, both in geographical and host range distribution. T. gondii is associated with severe birth defects and with opportunistic infections in AIDS patients. T. gondii contains three types of secretory organelles: micronemes, rhoptries and dense granules ...
... Apicomplexa, but T. gondii is the most widespread, both in geographical and host range distribution. T. gondii is associated with severe birth defects and with opportunistic infections in AIDS patients. T. gondii contains three types of secretory organelles: micronemes, rhoptries and dense granules ...
An A/ENTH Domain-Containing Protein Functions
... Among a large number of ANTH domain proteins, AP180 is involved in endocytosis in both animal and plant cells (Mao et al., 2001; Barth and Holstein, 2004). Among the ENTH domain proteins, Epsin1, Epsin2, and Epsin3 in animal cells and ENT1 in yeast function in endocytosis (Chen et al., 1998; Aguilar ...
... Among a large number of ANTH domain proteins, AP180 is involved in endocytosis in both animal and plant cells (Mao et al., 2001; Barth and Holstein, 2004). Among the ENTH domain proteins, Epsin1, Epsin2, and Epsin3 in animal cells and ENT1 in yeast function in endocytosis (Chen et al., 1998; Aguilar ...
Evolutionary tradeoffs in cellular composition across
... One of the most important classic and contemporary interests in biology is the connection between cellular composition and physiological function. Decades of research have allowed us to understand the detailed relationship between various cellular components and processes for individual species, and ...
... One of the most important classic and contemporary interests in biology is the connection between cellular composition and physiological function. Decades of research have allowed us to understand the detailed relationship between various cellular components and processes for individual species, and ...
Viability and Cytotoxicity Assay Kits for Diverse Cell Types
... dead-cell population by simply adding the reagents, incubating for a brief period and observing the results without any wash steps required. These facile assays are ideal for high-throughput screening applications and, in most cases, for imaging, fluorometry and flow cytometry. Our unique LIVE/DEAD ...
... dead-cell population by simply adding the reagents, incubating for a brief period and observing the results without any wash steps required. These facile assays are ideal for high-throughput screening applications and, in most cases, for imaging, fluorometry and flow cytometry. Our unique LIVE/DEAD ...
That Impairs Th1-Type Immunity Down
... synthesis (19, 20). These data further support the supposition that the combined production of the immunosuppressants acts to downmodulate host anti-M. tuberculosis immunity and thereby allow uncontrolled bacterial replication and overt disease. We sought additional host mediators not previously eva ...
... synthesis (19, 20). These data further support the supposition that the combined production of the immunosuppressants acts to downmodulate host anti-M. tuberculosis immunity and thereby allow uncontrolled bacterial replication and overt disease. We sought additional host mediators not previously eva ...
FoxF is essential for FGF-induced migration of heart
... transcriptional target of the FGF/MAPK/Ets pathway in the B7.5 lineage. To test this hypothesis, we isolated and characterized FoxF cis-regulatory sequences. The genomic region upstream of the first FoxF exon is highly conserved between Ciona intestinalis and Ciona savignyi (Fig. 2A), which often po ...
... transcriptional target of the FGF/MAPK/Ets pathway in the B7.5 lineage. To test this hypothesis, we isolated and characterized FoxF cis-regulatory sequences. The genomic region upstream of the first FoxF exon is highly conserved between Ciona intestinalis and Ciona savignyi (Fig. 2A), which often po ...
Corresponding author: Dr. A. Lacey Samuels
... these levels were corrected to the mean white of the background, to get average density values in defined cell types (secondary cell wall/cortical cytoplasm of developing tracheids; cytoplasm of rays) under different inhibitory conditions (Figure 3). In developing tracheids, inhibition of C4H by 10 ...
... these levels were corrected to the mean white of the background, to get average density values in defined cell types (secondary cell wall/cortical cytoplasm of developing tracheids; cytoplasm of rays) under different inhibitory conditions (Figure 3). In developing tracheids, inhibition of C4H by 10 ...
A Simple 1-D Physical Model for the Crawling Nematode Sperm Cell
... Biochemical Regulation. The precise role played by pH in MSP turnover, bundling and adhesion is yet to be established. In the model, we use pH distribution as a marker for these processes. We assume that protons constantly appear at the cell body, and that protons leak out from the lamellipod, maint ...
... Biochemical Regulation. The precise role played by pH in MSP turnover, bundling and adhesion is yet to be established. In the model, we use pH distribution as a marker for these processes. We assume that protons constantly appear at the cell body, and that protons leak out from the lamellipod, maint ...
TGF beta signalling and its role in tumour pathogenesis
... TGF-β is a multifunctional cytokine that regulates cell proliferation, differentiation and extracellular matrix production (Jennings & Pietenpol, 1998; Verrecchia & Mauviel, 2002). Deregulation of TGFβ expression or signalling has been implicated in the pathogenesis of a variety of diseases, includin ...
... TGF-β is a multifunctional cytokine that regulates cell proliferation, differentiation and extracellular matrix production (Jennings & Pietenpol, 1998; Verrecchia & Mauviel, 2002). Deregulation of TGFβ expression or signalling has been implicated in the pathogenesis of a variety of diseases, includin ...
Chapter 2 - William Stallings, Data and Computer
... • takes ~2.8x10-6 seconds to insert single cell • time to traverse network depends on propagation delay and switching delay • assume propagation at two-thirds speed of light • if source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds • given implicit congestion control, ...
... • takes ~2.8x10-6 seconds to insert single cell • time to traverse network depends on propagation delay and switching delay • assume propagation at two-thirds speed of light • if source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds • given implicit congestion control, ...
Research Template - UMKC School of Medicine
... They are early in cell proliferation They express many important proteins (adhesion, extracellular matrix) Adventitia secretes chemokines, cytokines, angiogenic proteins that effect other cell walls In summary, the adventitia regulates vascular structure and function ...
... They are early in cell proliferation They express many important proteins (adhesion, extracellular matrix) Adventitia secretes chemokines, cytokines, angiogenic proteins that effect other cell walls In summary, the adventitia regulates vascular structure and function ...
Tensile Properties of Arabidopsis Cell Walls Depend on Both a
... composition to the mechanical properties of an organ, we must account for the contributions of turgor, cell-cell adhesion, the relative density of the foam (distribution of material between symplast and apoplast), and cell wall structure. The availability of Arabidopsis mutants with cell wall phenot ...
... composition to the mechanical properties of an organ, we must account for the contributions of turgor, cell-cell adhesion, the relative density of the foam (distribution of material between symplast and apoplast), and cell wall structure. The availability of Arabidopsis mutants with cell wall phenot ...
Archives of Microbiology
... allowing the microbes to scavenge nutrients during the colonization of animal hosts. However, the expression of putative virulence factors by bacterial cells exposed to hostile environments may be a strategy that microbes use to become successful commensal and pathogenic organisms. Thus, virulent de ...
... allowing the microbes to scavenge nutrients during the colonization of animal hosts. However, the expression of putative virulence factors by bacterial cells exposed to hostile environments may be a strategy that microbes use to become successful commensal and pathogenic organisms. Thus, virulent de ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.