Harmonin Mutations Cause Mechanotransduction Defects in
... gated by tip links, the extracellular filaments that consist of cadherin 23 (CDH23) and protocadherin 15 (PCDH15) and connect the stereocilia of each hair cell. However, which molecules mediate cadherin function at tip links is not known. Here we show that the PDZ-domain protein harmonin is a compon ...
... gated by tip links, the extracellular filaments that consist of cadherin 23 (CDH23) and protocadherin 15 (PCDH15) and connect the stereocilia of each hair cell. However, which molecules mediate cadherin function at tip links is not known. Here we show that the PDZ-domain protein harmonin is a compon ...
Idh paper revised II - G3: Genes | Genomes | Genetics
... (Thermo Scientific), along with a freshly prepared ATP standard series. Three replicates were assayed for each sample and three readings were conducted for each experiment. ATP concentration was determined by comparison to the ATP standard curve and normalized to total protein. ...
... (Thermo Scientific), along with a freshly prepared ATP standard series. Three replicates were assayed for each sample and three readings were conducted for each experiment. ATP concentration was determined by comparison to the ATP standard curve and normalized to total protein. ...
Prevention of gastrointestinal tumors based on adenomatous
... and platelet dysfunction (10, 11). Recent advances in immunology and increasing knowledge about tumor immunity are encouraging the development of various immunotherapies to eradicate tumors or prevent tumor development. Tumor-associated antigens (TAAs) have been identified that are recognized by spe ...
... and platelet dysfunction (10, 11). Recent advances in immunology and increasing knowledge about tumor immunity are encouraging the development of various immunotherapies to eradicate tumors or prevent tumor development. Tumor-associated antigens (TAAs) have been identified that are recognized by spe ...
jxb.oxfordjournals.org
... AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased (Supplementary Fig. S1J), indicating that miR396 negatively regulates the cell proliferation in leaves by repressing the expression of AtGRF genes, consistent with reported results (Liu et al., 2009; Rodriguez et al., 2010). ...
... AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased (Supplementary Fig. S1J), indicating that miR396 negatively regulates the cell proliferation in leaves by repressing the expression of AtGRF genes, consistent with reported results (Liu et al., 2009; Rodriguez et al., 2010). ...
Article PDF
... of lactic acid from pyruvate (Shim et al. 1997). The relevance of LDHA function for tumourigenicity was recently demonstrated in an RNAi-approach. Repression of LDHA stimulated OXPHOS and compromised the ability to proliferate during hypoxia (Fantin et al. 2006). C-myc-transformed cells were found t ...
... of lactic acid from pyruvate (Shim et al. 1997). The relevance of LDHA function for tumourigenicity was recently demonstrated in an RNAi-approach. Repression of LDHA stimulated OXPHOS and compromised the ability to proliferate during hypoxia (Fantin et al. 2006). C-myc-transformed cells were found t ...
The endocytosis of cellulose synthase in
... membrane. Our results suggest that CESA represents a new class of CME cargo ...
... membrane. Our results suggest that CESA represents a new class of CME cargo ...
Ocular Delivery of peptides and proteins
... related macular degeneration, proliferative diabetic retinopathy, etc. Loss to systemic circulation must be minimized ...
... related macular degeneration, proliferative diabetic retinopathy, etc. Loss to systemic circulation must be minimized ...
Factor-1-Deficient Mice IL-15-Deficient and IFN Regulatory 3 Cells
... IL-7⫺/⫺ and IL-7R⫺/⫺ mice have major defects in T cell development, but NK cell development is not compromised (8, 9). IL-7 and IL-7R play a critical role in lymphopoiesis by inducing survival and proliferation of progenitor T lymphocytes (10). Studies in IL-15⫺/⫺ mice and IL-15R␣⫺/⫺ mice have confi ...
... IL-7⫺/⫺ and IL-7R⫺/⫺ mice have major defects in T cell development, but NK cell development is not compromised (8, 9). IL-7 and IL-7R play a critical role in lymphopoiesis by inducing survival and proliferation of progenitor T lymphocytes (10). Studies in IL-15⫺/⫺ mice and IL-15R␣⫺/⫺ mice have confi ...
Endocytosis unplugged: multiple ways to enter the cell
... sequential action of various small actin-remodeling GTPases [12]. The two modes also differ in their requirements for Rho-GTPases, in that FcR-mediated phagocytosis relies on Rac1 and Cdc42 activities, whereas CR3mediated entry is specifically dependent on RhoA [12, 13]. The host phagocytic machiner ...
... sequential action of various small actin-remodeling GTPases [12]. The two modes also differ in their requirements for Rho-GTPases, in that FcR-mediated phagocytosis relies on Rac1 and Cdc42 activities, whereas CR3mediated entry is specifically dependent on RhoA [12, 13]. The host phagocytic machiner ...
The connection of cytoskeletal network with plasma membrane and
... spatially and temporarily regulating the transportation and deposition of cell wall components. This tight control is achieved by the dynamic behavior of the cytoskeletons, but also through the tethering of these structures to the plasma membrane. This tethering may also extend beyond the plasma mem ...
... spatially and temporarily regulating the transportation and deposition of cell wall components. This tight control is achieved by the dynamic behavior of the cytoskeletons, but also through the tethering of these structures to the plasma membrane. This tethering may also extend beyond the plasma mem ...
Control of organ shape - Development
... complex developmental process where multiple levels of regulation have to be coordinated spatially and temporally. Several questions remain unanswered regarding organ formation. (1) What early signaling events select the correct site within the primordial field for the initial cell shape changes? (2 ...
... complex developmental process where multiple levels of regulation have to be coordinated spatially and temporally. Several questions remain unanswered regarding organ formation. (1) What early signaling events select the correct site within the primordial field for the initial cell shape changes? (2 ...
ATM - dump.no
... types of traffic simultaneously, including realtime voice, video, and bursty TCP traffic Way in which data flow is handled depends on the characteristics of the traffic flow and requirements of the application (ex. Real-time video must be delivered within minimum variation in delay) Primary serv ...
... types of traffic simultaneously, including realtime voice, video, and bursty TCP traffic Way in which data flow is handled depends on the characteristics of the traffic flow and requirements of the application (ex. Real-time video must be delivered within minimum variation in delay) Primary serv ...
Tracing Hematopoietic Precursor Migration to Successive
... from mesoderm, in the yolk sac for most vertebrate species. The later hematopoietic sites give rise to more diverse progeny, including lymphoid cells. In the adult, the continuous production of all blood cell lineages relies on the existence of hematopoietic stem cells (HSCs). HSCs, i.e., cells with ...
... from mesoderm, in the yolk sac for most vertebrate species. The later hematopoietic sites give rise to more diverse progeny, including lymphoid cells. In the adult, the continuous production of all blood cell lineages relies on the existence of hematopoietic stem cells (HSCs). HSCs, i.e., cells with ...
DLG5 in Cell Polarity Maintenance and Cancer Development
... and interacting with other polarity proteins such as N-cadherin and β-catenin. DLG5 also participates in cell division and cell fate determination. DLG5 may serve as a tumor suppressor. Knockdown of DLG5 can induce loss of epithelial polarity and promote cancer development, cell proliferation, invas ...
... and interacting with other polarity proteins such as N-cadherin and β-catenin. DLG5 also participates in cell division and cell fate determination. DLG5 may serve as a tumor suppressor. Knockdown of DLG5 can induce loss of epithelial polarity and promote cancer development, cell proliferation, invas ...
Asymmetric cell division in C. elegans - Development
... and allowed to feed for 30 minutes before mounting for ablation. Thus, the staged animals were 0.5 to 1 hour old at the start of ablations. The animals were ablated over the course of the next 30 minutes, so that all ablations were complete by 1.5 hours after hatching. Individual cells were killed u ...
... and allowed to feed for 30 minutes before mounting for ablation. Thus, the staged animals were 0.5 to 1 hour old at the start of ablations. The animals were ablated over the course of the next 30 minutes, so that all ablations were complete by 1.5 hours after hatching. Individual cells were killed u ...
- Wiley Online Library
... which yielded Kd and coefficients (n) of 32 3.2 mM and 4.4 and 23.7 2.5 mM and 7 for rapid and slow endocytosis, respectively (Figure1D). Typically, a Hill equation is used for chemical reactions whose detailed intermediate steps are not known but for which co-operative behavior is suspected, an ...
... which yielded Kd and coefficients (n) of 32 3.2 mM and 4.4 and 23.7 2.5 mM and 7 for rapid and slow endocytosis, respectively (Figure1D). Typically, a Hill equation is used for chemical reactions whose detailed intermediate steps are not known but for which co-operative behavior is suspected, an ...
Synthesis and sorting of proteoglycans
... Particular sulphation patterns in the GAG chains allow interactions, normally of ionic nature, with growth factors, for example. Recent studies have identified ~30 PG protein cores. These cores are not just scaffolds for GAGs: they contain domains that have particular biological activities (Iozzo, 1 ...
... Particular sulphation patterns in the GAG chains allow interactions, normally of ionic nature, with growth factors, for example. Recent studies have identified ~30 PG protein cores. These cores are not just scaffolds for GAGs: they contain domains that have particular biological activities (Iozzo, 1 ...
Antimicrobial Agents and Chemotherapy
... the growth of B. cereus was different. An antibiotic concentration of 0.1 ,ug per ml decreased the growth rate after 30 min, as a result of lysis of part of the cells; resumption of growth followed, probably caused by growth of the cells that escaped the lysis. Gram-stained preparations showed that ...
... the growth of B. cereus was different. An antibiotic concentration of 0.1 ,ug per ml decreased the growth rate after 30 min, as a result of lysis of part of the cells; resumption of growth followed, probably caused by growth of the cells that escaped the lysis. Gram-stained preparations showed that ...
Targeting of Salmonella typhimurium to Vesicles Containing
... (SCV) upon entry into the host cell. In this study we have examined the intracellular trafficking route of S. typhimurium within epithelial cells. Indirect immunofluorescence analysis showed that bacteria initiated fusion with lysosomal membrane glycoprotein (lgp)-containing compartments "o15 min af ...
... (SCV) upon entry into the host cell. In this study we have examined the intracellular trafficking route of S. typhimurium within epithelial cells. Indirect immunofluorescence analysis showed that bacteria initiated fusion with lysosomal membrane glycoprotein (lgp)-containing compartments "o15 min af ...
... morphonuclear leucocytes (PMNLs) [8–10], and attenuated the production of interleukin-1 and tumor necrosis factor by human mononuclear cells [11]. In in vivo studies, ambroxol diminished the bleomycin-induced lung injury in rats [12], and decreased mortality after administration of paraquat, a herbi ...
A rapid and robust assay for detection of S
... effectively be determined by flow cytometry after isolation of nuclei. In animal cell cultures, high quality and intact nuclei preparations can be relatively easily obtained in hypotonic buffers with detergents. In plants, however, cell walls pose a significant barrier for nuclei isolation. To allev ...
... effectively be determined by flow cytometry after isolation of nuclei. In animal cell cultures, high quality and intact nuclei preparations can be relatively easily obtained in hypotonic buffers with detergents. In plants, however, cell walls pose a significant barrier for nuclei isolation. To allev ...
Evidence for inducible recruitment of Wiskott
... membranes is mediated by the SLP-76 protein.14 In addition, molecular imaging analysis also reveals the co-localization of WASp with TCR-associated protein tyrosine kinase ZAP-70 (ζ-chain-associated protein of 70 kDa) after T cell activation, indicating that WASp is recruited to the TCR activation s ...
... membranes is mediated by the SLP-76 protein.14 In addition, molecular imaging analysis also reveals the co-localization of WASp with TCR-associated protein tyrosine kinase ZAP-70 (ζ-chain-associated protein of 70 kDa) after T cell activation, indicating that WASp is recruited to the TCR activation s ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.