Molecular Identification of a SNAP-25
... were amplified by PCR using the PtSNAP-specific oligonucleotides (Table 1) and cloned into the double T7 promoter plasmid pL4440 (71) over the SpeI and XhoI restriction sites. Plasmids were introduced in the E. coli Ht115 strain, and Paramecium cells were fed with these strains as described in detai ...
... were amplified by PCR using the PtSNAP-specific oligonucleotides (Table 1) and cloned into the double T7 promoter plasmid pL4440 (71) over the SpeI and XhoI restriction sites. Plasmids were introduced in the E. coli Ht115 strain, and Paramecium cells were fed with these strains as described in detai ...
The Relationship between Methylmercury Dosages and Amount of
... of bright green shapes were apparent, especially in the glial cell regions determined by the images under transmitted light. While it was evident that endocytic activity had been occurring in these glial cells, the exact number of endosomes could not be quantified because they were often clumped tog ...
... of bright green shapes were apparent, especially in the glial cell regions determined by the images under transmitted light. While it was evident that endocytic activity had been occurring in these glial cells, the exact number of endosomes could not be quantified because they were often clumped tog ...
Virtual Cell Client
... Some of the new features of the revised VCell Client include: The client uses Java3D technology to display the 3D scene. It is designed to be browser independent. You can use almost any browser to run the VCell program. ...
... Some of the new features of the revised VCell Client include: The client uses Java3D technology to display the 3D scene. It is designed to be browser independent. You can use almost any browser to run the VCell program. ...
BOSTON UNIVERSITY SCHOOL OF MEDICINE Thesis THE ROLE
... a much lesser extent by the retina (Cahill 1992; Gothilf 1999). Its production and release are tightly coupled with the circadian clock. Light input of SCN neurons is complemented by a feedback loop involving the rhythmic release of melatonin from the pineal gland (Vallone 2012; Von Gall 2002; Gille ...
... a much lesser extent by the retina (Cahill 1992; Gothilf 1999). Its production and release are tightly coupled with the circadian clock. Light input of SCN neurons is complemented by a feedback loop involving the rhythmic release of melatonin from the pineal gland (Vallone 2012; Von Gall 2002; Gille ...
Physico-chemical characteristics of cell walls from Arabidopsis
... types of glycosyl residues giving type II rhamnogalacturonan (RGII) (Ridley et al., 2001; Vincken et al., 2003). RGI has a backbone of alternating galacturonic acid and rhamnose residues with 20–80% of the rhamnose residues substituted with side chains of (1,4)-b-D-galactans, (1,5)a-L-arabinans, or ...
... types of glycosyl residues giving type II rhamnogalacturonan (RGII) (Ridley et al., 2001; Vincken et al., 2003). RGI has a backbone of alternating galacturonic acid and rhamnose residues with 20–80% of the rhamnose residues substituted with side chains of (1,4)-b-D-galactans, (1,5)a-L-arabinans, or ...
B3 Homework and answers
... wound around one another in the form of a double _______________. Four chemical bases, represented by the letters A, _______________, C and T, make up the code. In groups of _______________, these bases determine the order in which amino acids are joined together to form the many tens of thousands o ...
... wound around one another in the form of a double _______________. Four chemical bases, represented by the letters A, _______________, C and T, make up the code. In groups of _______________, these bases determine the order in which amino acids are joined together to form the many tens of thousands o ...
Chapter 7 PPT
... Fluid Mosaic Model In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer ...
... Fluid Mosaic Model In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer ...
Adducin-1 is essential for mitotic spindle assembly through its
... cell–cell adhesion (Abdi and Bennett, 2008; Naydenov and Ivanov, 2010). The ADD family consists of three closely related genes: ...
... cell–cell adhesion (Abdi and Bennett, 2008; Naydenov and Ivanov, 2010). The ADD family consists of three closely related genes: ...
Epidermal Pavement Cells of Arabidopsis Have
... autofluorescent mesophyll chloroplasts. Since pavement cell chloroplasts display considerably lower autofluorescence, their fluorescent signal may fall below the detection range in this circumstance. Another factor requiring consideration in the context of pavement cell chloroplasts is the intrinsic ...
... autofluorescent mesophyll chloroplasts. Since pavement cell chloroplasts display considerably lower autofluorescence, their fluorescent signal may fall below the detection range in this circumstance. Another factor requiring consideration in the context of pavement cell chloroplasts is the intrinsic ...
Supplementary Notes - Word file
... regions (Supplemental Fig. 2a-c) were not amenable to gel-digest LC-MS/MS analysis, most likely due to incompatibility with reverse-phase liquid chromatography (peptides which were too hydrophilic or too hydrophobic) or poor peptide fragmentation resulting in low-quality MS/MS spectra. The overall c ...
... regions (Supplemental Fig. 2a-c) were not amenable to gel-digest LC-MS/MS analysis, most likely due to incompatibility with reverse-phase liquid chromatography (peptides which were too hydrophilic or too hydrophobic) or poor peptide fragmentation resulting in low-quality MS/MS spectra. The overall c ...
Force is a signal that cells cannot ignore
... By definition, a signal can be transmitted, routed, and transduced, and each of these steps can be a point for regulation. The study of cellular signaling has traditionally rested on biochemical concepts, in which chemical signals are transmitted via diffusion, routed by specific binding interaction ...
... By definition, a signal can be transmitted, routed, and transduced, and each of these steps can be a point for regulation. The study of cellular signaling has traditionally rested on biochemical concepts, in which chemical signals are transmitted via diffusion, routed by specific binding interaction ...
localization of the succinic dehydrogenase system
... within the nuclear area (Figs. 6 and 9). In cases where the deposition of T N F was not heavy, it was possible to localize specifically a site of SDH activity on the plasma m e m b r a n e (x, Fig. 4). In addition, in cells having undergone partial plasmolysis with retraction of the cell membrane fr ...
... within the nuclear area (Figs. 6 and 9). In cases where the deposition of T N F was not heavy, it was possible to localize specifically a site of SDH activity on the plasma m e m b r a n e (x, Fig. 4). In addition, in cells having undergone partial plasmolysis with retraction of the cell membrane fr ...
DeadEnd Fluorometric TUNEL System Technical
... In many cell types, apoptosis is characterized by the generation of DNA fragments through the action of endogenous endonucleases (11–14). The DNA of apoptotic cells is cleaved into multimers of 180–200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typ ...
... In many cell types, apoptosis is characterized by the generation of DNA fragments through the action of endogenous endonucleases (11–14). The DNA of apoptotic cells is cleaved into multimers of 180–200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typ ...
The Antiglobulin Test
... Fetus must be antigen positive. Fetal cells become coated with maternal antibody & are cleared by the fetal RES. ...
... Fetus must be antigen positive. Fetal cells become coated with maternal antibody & are cleared by the fetal RES. ...
Promoter DNA methylation couples genome
... genes whose expression is primarily and causally regulated by promoter DNA methylation in different cell types, we generated and integrated gene expression datasets from multiple experimental conditions in which genome-wide loss of DNA methylation is induced in different ways. We initially developed ...
... genes whose expression is primarily and causally regulated by promoter DNA methylation in different cell types, we generated and integrated gene expression datasets from multiple experimental conditions in which genome-wide loss of DNA methylation is induced in different ways. We initially developed ...
Membrane dynamics of dividing cells imaged by lattice light
... emitted fluorescence signal is observed along an optical axis orthogonal to the illumination plane. Exposure (and consequent photodamage) is thus restricted to the thin slice of the cell being imaged at any moment, and the image is uncorrupted by out-of-focus signal. The thinness of the light sheet ...
... emitted fluorescence signal is observed along an optical axis orthogonal to the illumination plane. Exposure (and consequent photodamage) is thus restricted to the thin slice of the cell being imaged at any moment, and the image is uncorrupted by out-of-focus signal. The thinness of the light sheet ...
From profiles to function in epigenomics
... cal limitations. Although more than 100 different RNA modifications are known33, new modifications are continuing to be discovered34–36 and they have not yet been comprehensively profiled across the transcriptome33,37. Further advanced is the systematic profiling of chromatin architecture, which onl ...
... cal limitations. Although more than 100 different RNA modifications are known33, new modifications are continuing to be discovered34–36 and they have not yet been comprehensively profiled across the transcriptome33,37. Further advanced is the systematic profiling of chromatin architecture, which onl ...
Slide 1
... • X-gal overlay assay is not suitable for spores, instead βgalactosidase activity assay using ONPG. • Transcription factors regulating late sporulation induced genes are not yet identified. Finding regulators of AQY1 will point to transcription factors that possibly regulates other late sporulation ...
... • X-gal overlay assay is not suitable for spores, instead βgalactosidase activity assay using ONPG. • Transcription factors regulating late sporulation induced genes are not yet identified. Finding regulators of AQY1 will point to transcription factors that possibly regulates other late sporulation ...
Dictyostelium cytokinesis: from molecules to mechanics
... different views of the mechanics of cytokinesis. In the equatorial force-generation model, the cleavage-furrow cortex ingresses through the active constriction of the actin contractile ring. The major generator of this force is myosin-II, an actin-activated, force-generating ATPase. This protein conv ...
... different views of the mechanics of cytokinesis. In the equatorial force-generation model, the cleavage-furrow cortex ingresses through the active constriction of the actin contractile ring. The major generator of this force is myosin-II, an actin-activated, force-generating ATPase. This protein conv ...
PDF
... the P. tepidariorum genome (Table 1), opens the possibility of investigating developmental processes in this spider on a genomewide scale. Key recent findings and their impact on the field Insights into anteroposterior axis formation and gastrulation ...
... the P. tepidariorum genome (Table 1), opens the possibility of investigating developmental processes in this spider on a genomewide scale. Key recent findings and their impact on the field Insights into anteroposterior axis formation and gastrulation ...
Euglena Coloring
... Euglenophyta. All euglena have chloroplasts and can make their own food by photosynthesis. They are not completely autotrophic though, euglena can also absorb food from their environment; euglena usually live in quiet ponds or puddles. Euglena move by a flagellum (plural ‚ flagella), which is a long ...
... Euglenophyta. All euglena have chloroplasts and can make their own food by photosynthesis. They are not completely autotrophic though, euglena can also absorb food from their environment; euglena usually live in quiet ponds or puddles. Euglena move by a flagellum (plural ‚ flagella), which is a long ...
the spider Parasteatoda tepidariorum
... the P. tepidariorum genome (Table 1), opens the possibility of investigating developmental processes in this spider on a genomewide scale. Key recent findings and their impact on the field Insights into anteroposterior axis formation and gastrulation ...
... the P. tepidariorum genome (Table 1), opens the possibility of investigating developmental processes in this spider on a genomewide scale. Key recent findings and their impact on the field Insights into anteroposterior axis formation and gastrulation ...
Endocytosis-like protein uptake in the bacterium Gemmata
... subcellular localization of different proteins after their internalization. Coincubation of Cy5-labeled fluorescent streptavidin or Cy3-labeled Ig with GFP resulted in colocalization of the two distinct proteins to the same compartment (Fig. S6). The proteins were found to be restricted to an outer r ...
... subcellular localization of different proteins after their internalization. Coincubation of Cy5-labeled fluorescent streptavidin or Cy3-labeled Ig with GFP resulted in colocalization of the two distinct proteins to the same compartment (Fig. S6). The proteins were found to be restricted to an outer r ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.