Novagen • pET System Manual • 11th Edition
... The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source ...
... The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source ...
Big_Idea_2.C.1
... Also, insulin encourages enzymes to convert glucose to glycogen for storage. If however, you have been doing a lot of exercise, and glucose is being used up, then alpha cells will produce glucagon, this causes the release of an enzyme that breaks glycogen to glucose ...
... Also, insulin encourages enzymes to convert glucose to glycogen for storage. If however, you have been doing a lot of exercise, and glucose is being used up, then alpha cells will produce glucagon, this causes the release of an enzyme that breaks glycogen to glucose ...
NF1X - BioMed Central
... consensus sequence TTGGC(N)5GCCAA [1], and has been shown to activate replication of adenoviral DNA [2]. It is highly conserved in vertebrates, with chicken and hamster orthologs showing 92% amino acid sequence identity [3]. NF1X is reported to control the expression of a number of different genes i ...
... consensus sequence TTGGC(N)5GCCAA [1], and has been shown to activate replication of adenoviral DNA [2]. It is highly conserved in vertebrates, with chicken and hamster orthologs showing 92% amino acid sequence identity [3]. NF1X is reported to control the expression of a number of different genes i ...
Transcription and the control of gene expression
... FIGURE 6.14. In eukaryotes, RNA polymerase II is guided to the promoter by TFII accessory proteins. (A) TBP binds to the TATA box. (B) The complete transcription preinitiation complex. (C) Phosphorylated RNA polymerase is active. ...
... FIGURE 6.14. In eukaryotes, RNA polymerase II is guided to the promoter by TFII accessory proteins. (A) TBP binds to the TATA box. (B) The complete transcription preinitiation complex. (C) Phosphorylated RNA polymerase is active. ...
Why We Need Systems Biology - Department of Computer Science
... amount of protein if efficiently transcribed (Meyer et al., 1978). However, this mRNA begins with the start codon for the lambda repressor protein and completely lacks a Shine-Dalgarno sequence, so it is very poorly translated (reviewed in Ptashne et al., 1980). The result is that there is much less ...
... amount of protein if efficiently transcribed (Meyer et al., 1978). However, this mRNA begins with the start codon for the lambda repressor protein and completely lacks a Shine-Dalgarno sequence, so it is very poorly translated (reviewed in Ptashne et al., 1980). The result is that there is much less ...
Symposium Poster - uospur
... Thank you to everyone in the Guillemin lab, my mentor Travis Wiles. Peter O’Day, Marilyn Drennan, all of the SPUR interns, and everyone else involved in SPUR. Thank you to the National Science foundation Research Experience for Undergraduates Site Program in Molecular Biosciences at the University o ...
... Thank you to everyone in the Guillemin lab, my mentor Travis Wiles. Peter O’Day, Marilyn Drennan, all of the SPUR interns, and everyone else involved in SPUR. Thank you to the National Science foundation Research Experience for Undergraduates Site Program in Molecular Biosciences at the University o ...
Gene Expression and Regulation
... replication and in transcription. Earlier we discussed how genes interact in transmission genetics, based on Mendelian principles. We will now address how gene expression is regulated, primarily at the level of transcription and translation and some of the ways in which we can use our current knowle ...
... replication and in transcription. Earlier we discussed how genes interact in transmission genetics, based on Mendelian principles. We will now address how gene expression is regulated, primarily at the level of transcription and translation and some of the ways in which we can use our current knowle ...
2081 Slc35a2 provides a novel role for glycosylation in glucose
... We found that Lec8 cells exhibit enhanced electrophoretic mobility of Glut1 protein, altered lectin binding, and reduced uptake of 2-NBDG, which was time- and concentration-dependent, and reduced whole cell ATP content. Importantly, we found that enhanced Glut1 electrophoretic mobility, altered lect ...
... We found that Lec8 cells exhibit enhanced electrophoretic mobility of Glut1 protein, altered lectin binding, and reduced uptake of 2-NBDG, which was time- and concentration-dependent, and reduced whole cell ATP content. Importantly, we found that enhanced Glut1 electrophoretic mobility, altered lect ...
pathologic-cplxs+operons - Bioinformatics Research Group at
... Compute log likelihood of two genes being WO or TUB pair based on intergenic distance. ...
... Compute log likelihood of two genes being WO or TUB pair based on intergenic distance. ...
Lactose Intolerance
... Lactose is broken into its monomer components by an enzyme called lactase. The polymers are broken into monomers during digestion and the monomers are then absorbed in the small intestine. Lactose intolerance is caused by missing or low levels of lactase enzyme in the small intestine where it is pro ...
... Lactose is broken into its monomer components by an enzyme called lactase. The polymers are broken into monomers during digestion and the monomers are then absorbed in the small intestine. Lactose intolerance is caused by missing or low levels of lactase enzyme in the small intestine where it is pro ...
Electronic supplementary material
... and in the direction of the reader. This helix is the only one which is not indicated by its number. On the right, the surface representations were made partially transparent in order to show the ribbon representations at the backbone. The comparison shows that the electrostatic surface potentials ...
... and in the direction of the reader. This helix is the only one which is not indicated by its number. On the right, the surface representations were made partially transparent in order to show the ribbon representations at the backbone. The comparison shows that the electrostatic surface potentials ...
Outline
... • Target ligands bear little resemblance to wild type and chemically different from each other. • To explore critical parameters of molecular recognition: – molecular shape, chirality, functional groups, internal flexibility,charge, and water solubility. ...
... • Target ligands bear little resemblance to wild type and chemically different from each other. • To explore critical parameters of molecular recognition: – molecular shape, chirality, functional groups, internal flexibility,charge, and water solubility. ...
Regulation & Mutations
... • Prokaryotes turn genes on and off by controlling transcription • Promoter • DNA segment that allows a gene to be transcribed • Helps RNA polymerase find where the gene starts • Operator • DNA segment that turns genes on or off • Operon • Region of DNA including the promoter, the operator, and gene ...
... • Prokaryotes turn genes on and off by controlling transcription • Promoter • DNA segment that allows a gene to be transcribed • Helps RNA polymerase find where the gene starts • Operator • DNA segment that turns genes on or off • Operon • Region of DNA including the promoter, the operator, and gene ...
problem set
... transcribed in a single polycistronic mRNA. In the E. coli trp operon, the five gene products all work together in the biosynthesis pathway for the amino acid tryptophan. It is advantageous to link genes together in an operon, so that only one promoter must be regulated to coordinate expression of t ...
... transcribed in a single polycistronic mRNA. In the E. coli trp operon, the five gene products all work together in the biosynthesis pathway for the amino acid tryptophan. It is advantageous to link genes together in an operon, so that only one promoter must be regulated to coordinate expression of t ...
Name three amino acids that are typically found at the
... normally difficult to break down and is a main part of the plant material used for bio-ethanol production. The genome of the bacterium is sequenced in the search of genes involved in cellulose breakdown, but no genes have homology (resemble) already known genes encoding proteins for cellulose breakd ...
... normally difficult to break down and is a main part of the plant material used for bio-ethanol production. The genome of the bacterium is sequenced in the search of genes involved in cellulose breakdown, but no genes have homology (resemble) already known genes encoding proteins for cellulose breakd ...
Sample Exam 3 answer key
... segregation for kan-resistance, since this is a dominant marker. Therefore, 3/4 will be kanamycin-resistant and 1/4 will be sensitive to kanamycin Plant B: Two independent insertions of the T-DNA occurred on different chromosomes. Therefore the two T-DNA insertions are unlinked. When plant B is self ...
... segregation for kan-resistance, since this is a dominant marker. Therefore, 3/4 will be kanamycin-resistant and 1/4 will be sensitive to kanamycin Plant B: Two independent insertions of the T-DNA occurred on different chromosomes. Therefore the two T-DNA insertions are unlinked. When plant B is self ...
2 C
... RNA polymerase has a low affinity for the promoter unless CAP (catabolite activator protein) is present When glucose levels are low cAMP concentration increases in prokaryotes ...
... RNA polymerase has a low affinity for the promoter unless CAP (catabolite activator protein) is present When glucose levels are low cAMP concentration increases in prokaryotes ...
RNA and Translation notes
... nucleus Structure polycistronic or monocistronic monocistronic Translation coupled separate from transcription ...
... nucleus Structure polycistronic or monocistronic monocistronic Translation coupled separate from transcription ...
Class VII Using cloned DNA fragments to study gene expression 1
... alleles or mutations) B. The “activity” of the same gene might be different in two different individuals (or tissues or organisms). This would cause differences in “gene expression”, and this in turn would cause differences in mRNA levels, which causes differences in protein quantities. Since protei ...
... alleles or mutations) B. The “activity” of the same gene might be different in two different individuals (or tissues or organisms). This would cause differences in “gene expression”, and this in turn would cause differences in mRNA levels, which causes differences in protein quantities. Since protei ...
Medical School Biochemistry
... The increased metabolic activity that occurs in muscle tissue during exercise causes hemoglobin to release more oxygen to the tissue for all of the following reasons EXCEPT: A. B. C. D. ...
... The increased metabolic activity that occurs in muscle tissue during exercise causes hemoglobin to release more oxygen to the tissue for all of the following reasons EXCEPT: A. B. C. D. ...
发现次级代谢途径特异性转录调控因子
... DNA failed to diminish the intensities of the pimJp retardation bands C:control reactions made with pure GST protein were negative in all cases, excluding a possible binding of this protein to the promoters ...
... DNA failed to diminish the intensities of the pimJp retardation bands C:control reactions made with pure GST protein were negative in all cases, excluding a possible binding of this protein to the promoters ...
The Biochemistry of Life
... hundred glucose residues (units). – amylopectin differs from amylose in being highly branched. At approximately every thirtieth residue along the chain, a short side chain is attached by a glycosidic bond to the #6 carbon atom (the carbon above the ring). ...
... hundred glucose residues (units). – amylopectin differs from amylose in being highly branched. At approximately every thirtieth residue along the chain, a short side chain is attached by a glycosidic bond to the #6 carbon atom (the carbon above the ring). ...
Lac operon
lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.