Unit 6B Learning Targets
... Transcription factors bind to specific DNA sequences and/or other regulatory proteins. b. Some of these transcription factors are activators (increase expression), while others are repressors (decrease expression). c. The combination of transcription factors binding to the regulatory regions at any ...
... Transcription factors bind to specific DNA sequences and/or other regulatory proteins. b. Some of these transcription factors are activators (increase expression), while others are repressors (decrease expression). c. The combination of transcription factors binding to the regulatory regions at any ...
File
... Some other regions act as binding sites for particular proteins, which in turn affect transcription of the nearby gene: • Enhancers are sequences that increase the rate of transcription (when a protein is bound to it) • Silencers inhibit transcription (when a protein is bound to it) ...
... Some other regions act as binding sites for particular proteins, which in turn affect transcription of the nearby gene: • Enhancers are sequences that increase the rate of transcription (when a protein is bound to it) • Silencers inhibit transcription (when a protein is bound to it) ...
Supplementary information
... The constitutive gene expression measurements from the NCI60 originate from three publicly available data sets, each independently generated on different experimental platforms. The Z-score normalized differential in constitutive gene expression across the NCI60 is treated in the same manner as GI50 ...
... The constitutive gene expression measurements from the NCI60 originate from three publicly available data sets, each independently generated on different experimental platforms. The Z-score normalized differential in constitutive gene expression across the NCI60 is treated in the same manner as GI50 ...
Biology 0200
... • tryptophan itself serves as a co-repressor for the operon • The five gene products of the operon (the proteins coded for by tryp E, tryp D, etc...) are enzymes in a pathway that converts chorismic acid to tryptophan. Answer, in a simple sentence or two, the following questions about the operon: A) ...
... • tryptophan itself serves as a co-repressor for the operon • The five gene products of the operon (the proteins coded for by tryp E, tryp D, etc...) are enzymes in a pathway that converts chorismic acid to tryptophan. Answer, in a simple sentence or two, the following questions about the operon: A) ...
Satellite Workshop: Information Processing in the Biological
... Understanding how the parts work is also important. But it is not enough. We need to know how they work together. This is the systems approach. ...
... Understanding how the parts work is also important. But it is not enough. We need to know how they work together. This is the systems approach. ...
Slide 1
... a. The signals that control gene expression are different and prokaryotic promoter regions must be added to the vector. b. The genetic code differs because prokaryotes substitute the base uracil for thymine. c. Prokaryotic cells cannot transcribe introns because their genes do not have them. d. The ...
... a. The signals that control gene expression are different and prokaryotic promoter regions must be added to the vector. b. The genetic code differs because prokaryotes substitute the base uracil for thymine. c. Prokaryotic cells cannot transcribe introns because their genes do not have them. d. The ...
THE CONTROL OF GENE EXPRESSION
... THE CONTROL OF GENE EXPRESSION • ATTEMPTING TO EXPLAIN THE PROCESS OF DIFFERENTATION LIES IN DISCOVERING HOW EUKARYOTIC CELLS CONTROL GENE EXPRESSION • THIS IS DIFFICULT, BECAUSE EUKARYOTIC GENOMES ARE LARGE AND COMPLEX; BUT WE SHALL DO OUR BEST!!! (VICTORY WILL BE OURS!!) ...
... THE CONTROL OF GENE EXPRESSION • ATTEMPTING TO EXPLAIN THE PROCESS OF DIFFERENTATION LIES IN DISCOVERING HOW EUKARYOTIC CELLS CONTROL GENE EXPRESSION • THIS IS DIFFICULT, BECAUSE EUKARYOTIC GENOMES ARE LARGE AND COMPLEX; BUT WE SHALL DO OUR BEST!!! (VICTORY WILL BE OURS!!) ...
Chapter 18 PPT
... • The repressor can be in an active or inactive form, depending on the presence of other molecules • A corepressor is a molecule that cooperates with a repressor protein to switch an operon off ...
... • The repressor can be in an active or inactive form, depending on the presence of other molecules • A corepressor is a molecule that cooperates with a repressor protein to switch an operon off ...
CH7 DNAtoProtein
... operon to be turned “on”? • No glucose • Increasing levels of cAMP • cAMP binds to CAP, then complex binds next to lactose operon promoter at the activator region • RNA polymerase binds to promoter • Lactose present • Allolactose binds to repressor, keeping it from binding to the operator • RNA poly ...
... operon to be turned “on”? • No glucose • Increasing levels of cAMP • cAMP binds to CAP, then complex binds next to lactose operon promoter at the activator region • RNA polymerase binds to promoter • Lactose present • Allolactose binds to repressor, keeping it from binding to the operator • RNA poly ...
posterexample2
... The unwounded leaves of both wild type and mutant had low expression levels of lipoxygease 2, allene oxide synthase, OPDA reductase 3 and low induction of the JA responsive gene, PDF1.2. The JAZ1 gene that encodes for a protein that repress genes in the JA pathway was elevated in the unwounded tissu ...
... The unwounded leaves of both wild type and mutant had low expression levels of lipoxygease 2, allene oxide synthase, OPDA reductase 3 and low induction of the JA responsive gene, PDF1.2. The JAZ1 gene that encodes for a protein that repress genes in the JA pathway was elevated in the unwounded tissu ...
Document
... transcribed unless turned off by the regulator protein. The repressor is a tetramer of identical subunits of 38 kD each. There are ~10 tetramers in a wild-type cell. The repressor functions by binding to an operator (formally denoted Olac) at the start of the lacZYA cluster. The operator lies be ...
... transcribed unless turned off by the regulator protein. The repressor is a tetramer of identical subunits of 38 kD each. There are ~10 tetramers in a wild-type cell. The repressor functions by binding to an operator (formally denoted Olac) at the start of the lacZYA cluster. The operator lies be ...
Engineered Communications for Microbial Robotics
... Intracellular Circuits: The Inverter • In-vivo digital circuits: – signal = concentration of a specific protein – computation = regulated protein synthesis + decay ...
... Intracellular Circuits: The Inverter • In-vivo digital circuits: – signal = concentration of a specific protein – computation = regulated protein synthesis + decay ...
Exam 2 Full KEY v1 Bio200 Sum12
... should be as different from each other as is possible while still being specific and correct. Example) This mutation is in a gene that encodes a signaling molecule to start apoptosis. A random DNA polymerase III error in a white blood cell’s signal receptor gene causes the loss of social control so ...
... should be as different from each other as is possible while still being specific and correct. Example) This mutation is in a gene that encodes a signaling molecule to start apoptosis. A random DNA polymerase III error in a white blood cell’s signal receptor gene causes the loss of social control so ...
Power Point
... Promoters of the GAL7, GAL10 and GAL1 genes contain multiple binding sites for the Gal4p transcriptional activator ...
... Promoters of the GAL7, GAL10 and GAL1 genes contain multiple binding sites for the Gal4p transcriptional activator ...
Final Exam KEY
... c. (2) Suppose you succeeded in increasing the yield of fragments compared to sample B. Draw in the results on the gel in lane "C". d. (3) On a different day, you realize you forgot to add one of the primers to the tube. You stop the PCR after cycle 13, add the second primer and then let the PCR fin ...
... c. (2) Suppose you succeeded in increasing the yield of fragments compared to sample B. Draw in the results on the gel in lane "C". d. (3) On a different day, you realize you forgot to add one of the primers to the tube. You stop the PCR after cycle 13, add the second primer and then let the PCR fin ...
Chapter 10 Version #2 - Jamestown School District
... lactose located next to each other on DNA (can turn them on or off) – Genes on: they’re ready to be transcribed and translated ...
... lactose located next to each other on DNA (can turn them on or off) – Genes on: they’re ready to be transcribed and translated ...
GENETICS 603 Exam 1, September 27, 2013 1. Which of the
... C) Would regulation of the Ara-‐BAD operon be subject to cAMP levels? Justify your answer. ...
... C) Would regulation of the Ara-‐BAD operon be subject to cAMP levels? Justify your answer. ...
April 3 lecture slides
... Structures like this involving DNA with bound activator proteins and RNA polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
... Structures like this involving DNA with bound activator proteins and RNA polymerase complex are names “enhanceosomes”. TBP stands for TATAbinding protein, a component of RNA polymerase II associated factor, TFIID ...
Unit 3: Chemistry of Life
... >Saturated – means all Carbons have 2 hydrogen atoms bonded to it. >Unsaturated – means that some Carbons have double bonds (less H atoms) ...
... >Saturated – means all Carbons have 2 hydrogen atoms bonded to it. >Unsaturated – means that some Carbons have double bonds (less H atoms) ...
Positive Gene Regulation
... Genes that are not being expressed have a tendency to be heavily methylated Removal of the extra methyl groups can turn on certain genes. Experiments have shown that deficient DNA methylation due to lack of a methylating enzyme leads to abnormal embryotic development. In these cases, DNA methylation ...
... Genes that are not being expressed have a tendency to be heavily methylated Removal of the extra methyl groups can turn on certain genes. Experiments have shown that deficient DNA methylation due to lack of a methylating enzyme leads to abnormal embryotic development. In these cases, DNA methylation ...
Lac operon
lac operon (lactose operon) is an operon required for the transport and metabolism of lactose in Escherichia coli and many other enteric bacteria. Although glucose is the preferred carbon source for most bacteria, the lac operon allows for the effective digestion of lactose when glucose is not available. Gene regulation of the lac operon was the first genetic regulatory mechanism to be understood clearly, so it has become a foremost example of prokaryotic gene regulation. It is often discussed in introductory molecular and cellular biology classes at universities for this reason.Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. LacY encodes lactose permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes galactoside O-acetyltransferase. Layout of the lac operon.It would be wasteful to produce the enzymes when there is no lactose available or if there is a more preferable energy source available, such as glucose. The lac operon uses a two-part control mechanism to ensure that the cell expends energy producing the enzymes encoded by the lac operon only when necessary. In the absence of lactose, the lac repressor halts production of the enzymes encoded by the lac operon. In the presence of glucose, the catabolite activator protein (CAP), required for production of the enzymes, remains inactive, and EIIAGlc shuts down lactose permease to prevent transport of lactose into the cell. This dual control mechanism causes the sequential utilization of glucose and lactose in two distinct growth phases, known as diauxie.