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Sample Exam II
Sample Exam II

... types of questions you may expect, but realize that course content does change from year to year. Please review your lecture notes and also try the questions at the end of each chapter of your book. Answers are underlined. I sometimes make mistakes, so if something seems wrong feel free to contact m ...
DNA, RNA, and Protein Synthesis Notes 2006
DNA, RNA, and Protein Synthesis Notes 2006

... 3. Replication-the copying of DNA, occurs prior to cell division and is facilitated by DNA polymerases (special enzymes). 4. During replication a. the two strands of the double helix get unzipped b. matching bases are inserted c. sugar phosphate links are created 5. The scientists James Watson and ...
Discovery of the DNA molecule
Discovery of the DNA molecule

... nucleotide (GCTA) in the entire genomes of different species. Here’s what he found! ...
DNA Structure
DNA Structure

... Replication-The copying of DNA to make a new cell. DNA polymerase-the principle enzyme involved in replication which joins individual nucleotides to produce a DNA molecule (a polymer) Helicase- an enzyme that breaks the bonds between base pairs in DNA, leaving two rows of bases with free-ends, on wh ...
Lecture 9
Lecture 9

... Gene structure DNA replication ...
Microbial Genetics - University of Montana
Microbial Genetics - University of Montana

... • Lytic: anti-termination and early-middle-late genes • Lysogeny: repression of phage genes by cI protein • Lysis/lysogeny – Competition between regulators cII and Cro for operator sites » Host growth conditions ...
Slide 1
Slide 1

... Matthew Meselson and Franklin Stahl were a pair of scientists who decided to be unbiased, and focus on the 3 accepted, possible copying mechanisms at the time. The experiment they carried out is one of the most famous in Biology. ...
Questions - Humble ISD
Questions - Humble ISD

... 1. What is the shape of DNA? Who determined this shape? 2. What biomolecule does DNA belong to? 3. What is the monomer of DNA. 4. What are the 3 parts of the monomer? 5. A single-ringed N-base is called _____ & includes ________ & _______ 6. A double-ringed N-base is called ______ & includes _______ ...
Pipe cleaner DNA
Pipe cleaner DNA

... trogen bases, each attached to a 3 cm piece of tape. After separating the two DNA strands by unhooking the base pairs, complementary new bases are attached to each strand, ultimately forming two identical DNA molecules (Figure 3, p. 58). I encourage my students to make at least one error in this pr ...
Recombinant DNA Technology
Recombinant DNA Technology

... Basic ingredients needed for DNA Recombination: A. Know the gene of interest and the species carrying that gene B. Need a species that can rapidly replicate its DNA and divide C. Need a species containing many restriction sites on its DNA D. Need restriction enzymes—these enzymes are able to cut dou ...
DNA as Genetic Material
DNA as Genetic Material

... - Worked out DNA base pairing, explains Chargaff’s rule - Determined that DNA strands are antiparallel - finalized 3-d structure ...
File
File

... viruses called bacteriophages. (they infect bacteria!) •They knew viruses infect cells by injecting their own genetic material inside, but what is the genetic material? •Hershey and Chase radioactively marked the viral DNA. When the viruses infected bacteria, they saw that the bacteria now contained ...
dna
dna

... into fragments that can later be incorporated into another DNA strand; about 150 are commercially available Probe- A single strand of nucleic acid, much like RNA, that has been made in a way that its base sequence lines up to hybridize areas in an allele; usually labeled with radioactive material ...
DNA - Santa Susana High School
DNA - Santa Susana High School

... – bacteria have a single site while Eukaryotes have multiple sites – proteins recognize site and open up a replication bubble – as replication begins a replication forks form as replication proceeds in both directions • nucleoside triphosphates are added 1 at a time by DNA polymerase (~50/sec) in th ...
DNA Base Pairing Activity
DNA Base Pairing Activity

... 4. Depending on the size of the group, give them app. 5 minutes to pair up with another student.  a. If this is a group of students that are somewhat unfamiliar with each other, you could  also use this as an ice‐breaking activity. Have them introduce themselves to their pair  partner and tell them  ...
DNA structure
DNA structure

... – Complimentary DNA sequence – Carries DNA message from the nucleus to ribosomes ...
DNA damage and repair
DNA damage and repair

... of the DNA (e.g. G-C bp to methyl-G-C is DNA damage) •Mutation refers to a change in a base-pair (e.g. G-C bp to A-T bp is a mutation) •There are long term (inhertided) implications when DNA damage is converted to mutation ...
318 Conformational Elasticity Found to Facilitate TALE
318 Conformational Elasticity Found to Facilitate TALE

... experimentally determined apo and bound conformations. This elastic feature was also observed in the simulations starting from the apo form, which suggests low free energy barrier between the two conformations and small compensation required upon binding. To analyze the binding specificity, the rese ...
DNA REVIEW SHEET
DNA REVIEW SHEET

... 16. A codon that has no anticodon match would be called a ___________________. 17. What does DNA polymerase do? 18. Anything ending in –ase would be classified as an ____________________> 19. What 3 things make up DNA? 20. DNA is compared in structure to what? 21. What does DNA stand for? 22. How ma ...
Protein Synthesis: Transcription and Translation
Protein Synthesis: Transcription and Translation

... When a gene is expressed, DNA is transcribed to produce RNA and RNA is then translated to produce proteins. ...
SBI4U: DNA Replication - SBI4U with Ms. Taman!
SBI4U: DNA Replication - SBI4U with Ms. Taman!

... SBI4U: DNA Replication Why do we need to replicate our DNA? ____________________________ When does DNA replication happen in a Cell? _______________________ Background: Cell Division: ________________ + _________________ DNA is replicated in __________________ prior to mitosis Each _________________ ...
投影片 1
投影片 1

... nitrogenous bases and sugar and phosphate strands. Within the ladder model of DNA, the sugar and phosphate strands compose the sides of the DNA model, or molecule, while the actual rungs of the ladder are made up of the 4 nitrogen bases. ...
page 74-81
page 74-81

... together in the helical structure by the hydrogen bonds. page 77 1 Chargaff’s data showed that the proportions of guanine and cytosine are the same in DNA, as are the proportions of adenine and thymine. This must be the case if guanine forms complementary base pairs with cytosine and if adenine pair ...
Document
Document

... •Homologues of mutS and mutL genes exist so enzymes involved in eukaryotic mismatch repair likely to be similar to prokaryotic enzymes. •BUT, no homologue of MutH (protein that recognizes unmethylated newly synthesized strand) so recognition of newly synthesized strand does not appear to occur via a ...
交通大學特色研究計畫邀請 - 國立交通大學生物資訊研究所
交通大學特色研究計畫邀請 - 國立交通大學生物資訊研究所

... DNA molecule into distinct topological domains. Our approach is based on the successful construction of a series of plasmid DNA templates that contain many tandem copies of one or two DNA-binding sites in two different locations. With these approaches and atomic force microscopy, we discovered that ...
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Holliday junction



A Holliday junction is a branched nucleic acid structure that contains four double-stranded arms joined together. These arms may adopt one of several conformations depending on buffer salt concentrations and the sequence of nucleobases closest to the junction. The structure is named after the molecular biologist Robin Holliday, who proposed its existence in 1964.In biology, Holliday junctions are a key intermediate in many types of genetic recombination, as well as in double-strand break repair. These junctions usually have a symmetrical sequence and are thus mobile, meaning that the four individual arms may slide though the junction in a specific pattern that largely preserves base pairing. Additionally, four-arm junctions similar to Holliday junctions appear in some functional RNA molecules.Immobile Holliday junctions, with asymmetrical sequences that lock the strands in a specific position, were artificially created by scientists to study their structure as a model for natural Holliday junctions. These junctions also later found use as basic structural building blocks in DNA nanotechnology, where multiple Holliday junctions can be combined into specific designed geometries that provide molecules with a high degree of structural rigidity.
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