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Biol 115 DNA, the Thread of Life
Biol 115 DNA, the Thread of Life

... The amino acids specified by each mRNA codon. Multiple codons can code for the same amino acid. The codons are written 5' to 3', as they appear in the mRNA. AUG is an initiation codon; UAA, UAG, and UGA are termination (stop) codons. Biol115_2014_Lecture 7 ...
Molecular Orbital Interactions in the Anticodon of Transfer RNA
Molecular Orbital Interactions in the Anticodon of Transfer RNA

... Transport - vehicle for particle movement (e.g., transmembrane) Receptor - cell recognition and particle reception ...
Human Genetics--BIOL 102 Summer Lab 2--The
Human Genetics--BIOL 102 Summer Lab 2--The

... b. How many exons are in the gene? c. How many introns are in the gene? To help you answer later questions, please highlight the three exons. 2. Transcription is initiated by the binding of transcription factors to the promoter region at the front end of the gene. The promoter region usually include ...
Gene Expression
Gene Expression

... 2. The white molecule is a double stranded DNA molecule. Use the chain marked with an “X” as a template to construct an mRNA molecule with the yellow bases. (This simulation does not include introns or other control elements.) 3. Get a codon chart from the prep table or use the one in your textbook. ...
Transcription Activity Guide
Transcription Activity Guide

... 1. How many possible triplet codes can be generated from these four base letters? 64, calculation: 4 different bases, in groups of three, 43 = 64. _______________________________________________________________________________ _________________________________________________________________________ ...
The S RNA segment of tomato spotted wilt virus has an ambisense
The S RNA segment of tomato spotted wilt virus has an ambisense

... corresponds to a protein of 465 amino acids and an Mr of 52.4K. The amino acid sequence of this putative protein does not contain hydropfiobic regions that might function as signal peptides or transmembrane domains, according to the hydropathy algorithms of Hopp & Woods (1981) and Kyte & Doolittle ( ...
loading control antibodies for western blotting
loading control antibodies for western blotting

No Slide Title
No Slide Title

... http://bmb-itservices.bmb.psu.edu/bryant/lab/Project/Hydrogen/index.html#secti ...
Slide 1
Slide 1

... which provide the molecular basis for phenotypic traits  The connections between genes and proteins – In the 1940’s Beadle and Tatum suggested a one gene–one enzyme hypothesis based on studies of inherited metabolic diseases – Their hypothesis is still accepted but with important changes: ...
Transcription and Translation
Transcription and Translation

... how is it different visually from DNA? – It is single stranded – It is shorter and able to leave the nucleus – The sugar is ribose – There is a different base • Uracil (U) takes the place of Thymine (T) ...
Chapter 7: Microbial Genetics 10/8/2015
Chapter 7: Microbial Genetics 10/8/2015

... Regulation of Transcription The focal point is whether or not RNA polymerase binds the promoter of a gene and initiates transcription which depends on: 1) Affinity of RNA polymerase for a given promoter • some promoters are “strong” and bind RNA polymerase with high affinity • some promoters are “we ...
to linear sequence of 20 amino acids.
to linear sequence of 20 amino acids.

... convert mRNA sequence to amino acid sequence How many bases must be read at one time in order to have a unique code for each amino acid? ...
Microsoft Word
Microsoft Word

... Piwi is expressed in the germline and in early embryos. It plays a role in transposon silencing in the germplasm with the association of a species of small regulatory RNA (Saito et al, 2006) but little is known about its role in the early embryos. Since PIWI poses an interconnecting link between TGS ...
In experiments with a 3 base codon system it was shown that the
In experiments with a 3 base codon system it was shown that the

... A change in the base configuration is called a tautomeric shift where a base goes from the keto to the enol form. For example, if a tautomeric shift occurs in a thymine, it will be paired to guanine instead of adenine. If adenine undergoes a tautomeric shift it will now be read as a guanine and be p ...
Activation of Transcription
Activation of Transcription

... A typical differentiated mammalian cell makes about 100,000 proteins from approximately 35,000 genes. Most of these are housekeeping proteins needed to maintain all cell types. Certain proteins can only be detected in specific cell types. How is gene expression regulated? Regulation of gene express ...
DNA and Proteins - Furman University
DNA and Proteins - Furman University

epigenetics - Gene Silencing
epigenetics - Gene Silencing

... that double stranded RNA triggers suppression of gene activity in a homology-dependent manner, a process named RNA interference (RNAi). This discovery revealed a new mechanism for gene regulation and the biochemical machinery involved plays a key role in many essential cellular processes. This contr ...
Chapter 6: Genetic Control: DNA and RNA
Chapter 6: Genetic Control: DNA and RNA

... always from “ 5’ – 3’ ”. What does this means??? • As you start reading the nucleotides of a leading strand, you will realize a FREE phosphate grp is always attached to C5 of the pentose sugar. This group is called as the 5’ end of the chain • As you reach the end of the chain, you will see the fina ...
Bio Unit 7b DNA packet
Bio Unit 7b DNA packet

... 16. tRNA brings amino acids to the nucleus or ribosome? 17. A polypeptide is a sequence of proteins or amino acids? 18. tRNA has codons or anti-codons? 19. tRNA transfers amino acids during translation or transcription? 20. Ribosomes are the site where translation or transcription takes place? ...
DNA Polymerase
DNA Polymerase

The Importance of DNA and RNA - Emmanuel Biology 12
The Importance of DNA and RNA - Emmanuel Biology 12

... The unwound DNA exposes two parental strands of DNA which are antiparallel. This means they are orientated in different directions and must be replicated by different mechanisms. The leading strand elongates towards the replication fork (in the direction of unwinding) by the simple addition of nucle ...
mRNA
mRNA

... – They seem to facilitate the export of mRNA – They protect mRNA from hydrolytic enzymes – They help ribosomes attach to the 5 end Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings ...
DNA replication
DNA replication

... sequence of nitrogenous bases. DNA synthesis involves the complementary pairing of nucleotide bases on 2 strands of DNA. Mechanism by which genetic info is decoded and used to direct cellular processes begins with the synthesis of RNA. RNA synthesis- complimentary pairing of ribonucleotide bases wit ...
Translation Notes 2015 - Liberty Union High School District
Translation Notes 2015 - Liberty Union High School District

... that lets it find its spot on the mRNA strand.  Anti-codon: A region of tRNA consisting of 3 bases complementary to the mRNA codon (A with U, G with C) ...
week9_DNA&geneExpression.bak
week9_DNA&geneExpression.bak

... Patterns of Gene Expression • Bacteria directly exposed to environment • Respond to changes in nutrient availability ...
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Polyadenylation



Polyadenylation is the addition of a poly(A) tail to a messenger RNA The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.The process of polyadenylation begins as the transcription of a gene finishes, or terminates. The 3'-most segment of the newly made pre-mRNA is first cleaved off by a set of proteins; these proteins then synthesize the poly(A) tail at the RNA's 3' end. In some genes, these proteins may add a poly(A) tail at any one of several possible sites. Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing.The poly(A) tail is important for the nuclear export, translation, and stability of mRNA. The tail is shortened over time, and, when it is short enough, the mRNA is enzymatically degraded. However, in a few cell types, mRNAs with short poly(A) tails are stored for later activation by re-polyadenylation in the cytosol. In contrast, when polyadenylation occurs in bacteria, it promotes RNA degradation. This is also sometimes the case for eukaryotic non-coding RNAs.mRNA molecules in both prokaryotes and eukaryotes have polyadenylated 3'-ends, with the prokaryotic poly(A) tails generally shorter and less mRNA molecules polyadenylated.
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