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17-Gene to Protein
17-Gene to Protein

... • smallest units of uniform length to allow translation of all 20 amino acids • codon- triplet in mRNA ...
RNA and Protein Synthesis
RNA and Protein Synthesis

Genetic Controls in Eukaryotes
Genetic Controls in Eukaryotes

...  Regulation at post-transcriptional level - RNA processing o Alternative RNA splicing = different segments of RNA are treated as exons and introns = different mRNA o Controlled by regulatory proteins specific to each cell type o Consequence = a single gene can code for more than one polypeptide = ...
Document
Document

... • At the 5' end, a cap is added consisting of a modified GTP (guanosine triphosphate). • This occurs at the beginning of transcription. The 5' cap is used as a recognition signal for ribosomes to bind to the mRNA. • At the 3' end, a poly(A) tail of 150 or more adenine nucleotides is added. The tail ...
MATCH
MATCH

... b. _______________________ the  globin genes – embryonic, fetal, pseudo-, and adult c. _______________________ binding site for sister chromatids during mitosis d. _______________________ useful for DNA fingerprinting e. _______________________transcribed but not translated g. _____________________ ...
Chapter 17 - Denton ISD
Chapter 17 - Denton ISD

... 2) ____________- progresses in 5’-3’ direction a) Anticodon in tRNA bonds with the matching codon on mRNA at A site, using ____ for energy b) __________ bond forms between amino acids in A and P sites c) _______________- tRNA in A site moves to P site. Empty tRNA in P site moves to E site and then i ...
Expressing Genetic Information
Expressing Genetic Information

... 27. What are the cap and poly-A tail of the processed mRNA molecule? 28. Describe the process of translation. Where does it take place? What RNA’s are involved? 29. What are the three stages of translation? 30. What happens to the completed protein? 31. Read Focus On p. 253. What is the role of prot ...
Document
Document

... with 7-methylguanosine. This protects the transcript from degradation; capping is also necessary for translation of mature mRNA. ...
Reverse Transcription - St. Michael`s Hospital
Reverse Transcription - St. Michael`s Hospital

... activities: as a RNA‐dependent DNA polymerase, a DNA‐dependent DNA polymerase and ribonuclease  H. Many commercially available kits, such as Super Script® III (Invitrogen/Life Technologies) have  specifically engineered enzymes that possess reduced RNase H activity and provide increased thermal  sta ...
Lecture 4-5 Outline
Lecture 4-5 Outline

... Differential gene expression: e.g. of 30,000 genes in a typical mammalian cell, only about 5,000 are being transcribed at any time. Which 5000 depends on the cell type. Some are housekeeping genes, all cells transcribe them; some are specific to cell function, such as red blood cells where 95% of pr ...
Molecular Basis for Relationship between Genotype and Phenotype
Molecular Basis for Relationship between Genotype and Phenotype

The Molecular Genetics of Gene Expression
The Molecular Genetics of Gene Expression

... • Promoter = nucleotide sequence 20-200 bp long—is the initial binding site of RNA polymerase and transcription initiation factors ...
Ch 17 From Gene to Protei
Ch 17 From Gene to Protei

... 1. Transcription cannot begin in eukaryotic cells until a. the two DNA strands have completely separated and exposed the promoter. b. several transcription factors have bound to the promoter. c. the 5' caps are removed from the mRNA. d. the DNA introns are removed from the template. e. DNA nucleases ...
Chapter 10 Structure and Function of DNA
Chapter 10 Structure and Function of DNA

... How is DNA replication related to S- Phase? Primase Okazaki Fragments What is significant about the 3’-OH Why do chromosomes get shorter and shorter every round of replication? What are telomeres? What is telomerase? What happens if there is a mistake? What is the role of single-stranded binding pro ...
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Poly-Silicon

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The 11th lecture in molecular biology
The 11th lecture in molecular biology

Walk the Dogma - Nutley Public Schools
Walk the Dogma - Nutley Public Schools

... information is copied from DNA to RNA • DNA double-strand “unzips” • RNA polymerase (an enzyme) binds to a specific region on DNA called a promoter • RNA polymerase travels along the gene, creating a chain of mRNA that is complementary to the strand of DNA • RNA polymerase reaches the termination si ...
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... 1. Which of the following is not involved with initiation of transcription in human genes ...
Chapter 10 Protein Synthesis Test Study Guide THERE WILL BE 21
Chapter 10 Protein Synthesis Test Study Guide THERE WILL BE 21

... What is the relationship between a cell, DNA and protein? Explain. (p. 204) List the three types of RNA and their functions. (p. 205) List the four ways RNA differs from DNA. (p. 205) In RNA, the base adenine is complementary to the base ______________. (p. 205) How are DNA replication and transcrip ...
Three Types of RNA and Their Functions
Three Types of RNA and Their Functions

Crossword Puzzle: Protein Synthesis
Crossword Puzzle: Protein Synthesis

... 3. Sequence of nucleotides on DNA to with RNA polymerase will attach to start transcription 4. mRNA copying DNA's nucleotide sequence 5. 3 nucleotides on tRNA that match to a specific codon on mRNA 6. Type of RNA that helps make up ribosomes 7. Instructions for making proteins in cells 14. The numbe ...
Protein Synthesis - Biology Junction
Protein Synthesis - Biology Junction

... transcription 4. mRNA copying DNA's nucleotide sequence 5. 3 nucleotides on tRNA that match to a specific codon on mRNA 6. Type of RNA that helps make up ribosomes 7. Instructions for making proteins in cells 14. The number of amino acids that exist 16. Number of strands making up RNA 19. DNA to RNA ...
objective 3 - protein synthesis
objective 3 - protein synthesis

DNA Know your Molecules
DNA Know your Molecules

... Type of RNA made ...
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... 1. Which of the following is not involved with initiation of transcription in human genes ...
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Polyadenylation



Polyadenylation is the addition of a poly(A) tail to a messenger RNA The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.The process of polyadenylation begins as the transcription of a gene finishes, or terminates. The 3'-most segment of the newly made pre-mRNA is first cleaved off by a set of proteins; these proteins then synthesize the poly(A) tail at the RNA's 3' end. In some genes, these proteins may add a poly(A) tail at any one of several possible sites. Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing.The poly(A) tail is important for the nuclear export, translation, and stability of mRNA. The tail is shortened over time, and, when it is short enough, the mRNA is enzymatically degraded. However, in a few cell types, mRNAs with short poly(A) tails are stored for later activation by re-polyadenylation in the cytosol. In contrast, when polyadenylation occurs in bacteria, it promotes RNA degradation. This is also sometimes the case for eukaryotic non-coding RNAs.mRNA molecules in both prokaryotes and eukaryotes have polyadenylated 3'-ends, with the prokaryotic poly(A) tails generally shorter and less mRNA molecules polyadenylated.
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