... development 24 and 48 h post inoculation (Fig. 4A). PL secretion during the colonization of watermelon seedling stems by Cm-PL-3 and Cm-PL-10 was detected 48 h post inoculation, for Cg-14 and L-2.5 only after 120 h (Fig. 4B). Inoculation of watermelon seedlings with Cm-PL-10 and Cm-PL-3 caused dampi ...
Bio 6 – DNA & Gene Expression Lab Overview
... The Genetic Code Before we look at translation, you need to understand the genetic code. While this may seem like yet another thing you need to learn in biology class, having knowledge of the genetic code is truly a remarkable privilege. Scientists and thinkers from the past would have given anythin ...
... The Genetic Code Before we look at translation, you need to understand the genetic code. While this may seem like yet another thing you need to learn in biology class, having knowledge of the genetic code is truly a remarkable privilege. Scientists and thinkers from the past would have given anythin ...
Germline Mutation in NLRP2 (NALP2) in a Familial
... KvDMR1, we analysed methylation levels at the TND (6q24), SNRPN (15q13) and PEG1 (7q32) DMRs. Both affected siblings (and all controls) had normal methylation levels at the TND and SNRPN DMRs but Child 2 demonstrated partial loss of methylation at the PEG1 DMR (Figure 3). ...
... KvDMR1, we analysed methylation levels at the TND (6q24), SNRPN (15q13) and PEG1 (7q32) DMRs. Both affected siblings (and all controls) had normal methylation levels at the TND and SNRPN DMRs but Child 2 demonstrated partial loss of methylation at the PEG1 DMR (Figure 3). ...
Webinar presentation (.ppt) - The Cholangiocarcinoma Foundation
... Cekanova and Rathore. Animal models and therapeutic molecular targets of cancer: utility and limitations. Drug Design, Development and Therapy. 2014,8: 1911-22 Gopinathan et al. GEMMs as preclinical models for testing pancreatic cancer therapies. Dis Model & Mech. 2015,8: 1185-1200 Harno et al. Meta ...
... Cekanova and Rathore. Animal models and therapeutic molecular targets of cancer: utility and limitations. Drug Design, Development and Therapy. 2014,8: 1911-22 Gopinathan et al. GEMMs as preclinical models for testing pancreatic cancer therapies. Dis Model & Mech. 2015,8: 1185-1200 Harno et al. Meta ...
The dystrophin / utrophin homologues in Drosophila and in sea urchin
... cDNA primer was from exon 4. The antisense PCR primer that consisted of exons 3±4 border sequences, and sense primers that were speci®c to each dmDLP isoform. The PCR reaction was stopped after 27 cycles. The products were size fractionated and transferred to nylon ®lters. After hybridization to int ...
... cDNA primer was from exon 4. The antisense PCR primer that consisted of exons 3±4 border sequences, and sense primers that were speci®c to each dmDLP isoform. The PCR reaction was stopped after 27 cycles. The products were size fractionated and transferred to nylon ®lters. After hybridization to int ...
Loss of Heterozygosity at 6q Is Frequent and Concurrent with 3p
... of 6q was detected in one of these, showing that allelic losses at 6q is not specific to cerebellar hemangioblastomas. Several experimental studies have provided strong evidence on the importance of 6q in the process of tumorigenicity. The transfer of a normal chromosome 6 into breast and ovarian ca ...
... of 6q was detected in one of these, showing that allelic losses at 6q is not specific to cerebellar hemangioblastomas. Several experimental studies have provided strong evidence on the importance of 6q in the process of tumorigenicity. The transfer of a normal chromosome 6 into breast and ovarian ca ...
Reactome Functional Interaction (FI) Network
... Experiment: Gene expression profiling of pancreatic tumour (vs normal tissue). Output: list of genes that show significant changes in expression. Downstream analysis: 1. Validation of the top genes sorted by fold-change (or p-value): artificial cut-off, information loss; 2. Manual reviewing and gene ...
... Experiment: Gene expression profiling of pancreatic tumour (vs normal tissue). Output: list of genes that show significant changes in expression. Downstream analysis: 1. Validation of the top genes sorted by fold-change (or p-value): artificial cut-off, information loss; 2. Manual reviewing and gene ...
Inferring causal relationships among intermediate phenotypes and
... But it has not yet been applied to genetic analysis. The reason is simple: the causal structure in genetic data is known, i.e. genotype is the cause, phenotype is the effect, and the reverse Lamarckian relationship has been proven to be unlikely. The situation is changed, however, when the two varia ...
... But it has not yet been applied to genetic analysis. The reason is simple: the causal structure in genetic data is known, i.e. genotype is the cause, phenotype is the effect, and the reverse Lamarckian relationship has been proven to be unlikely. The situation is changed, however, when the two varia ...
CHAPTER 15 THE CHROMOSOMAL BASIS OF INHERITANCE
... are inherited from the mother or the father (imprinting) 3. Extranuclear genes exhibit a non-Mendelian pattern of inheritance ...
... are inherited from the mother or the father (imprinting) 3. Extranuclear genes exhibit a non-Mendelian pattern of inheritance ...
The Genetics of Beta-galactosidase
... wishes to test if the expression of a certain gene is controlled by environmental factors, a gene fusion with a promoter-less β-galactosidase can be used. This is especially useful if the gene product is uncharacterized or is not easily assayed. Along the same lines, the rate of gene expression can ...
... wishes to test if the expression of a certain gene is controlled by environmental factors, a gene fusion with a promoter-less β-galactosidase can be used. This is especially useful if the gene product is uncharacterized or is not easily assayed. Along the same lines, the rate of gene expression can ...
The Canine Genome: Discoveries, Applications - Encompass
... 46 chromosomes—is contained in all cells that have a nucleus. The mitochondrion, the site of ATP production in the cell, contains a circular chromosome that encodes approximately 17 mitochondrial proteins and several transfer RNA genes. In canines, the nuclear genome contains 2.8 billion base pairs ...
... 46 chromosomes—is contained in all cells that have a nucleus. The mitochondrion, the site of ATP production in the cell, contains a circular chromosome that encodes approximately 17 mitochondrial proteins and several transfer RNA genes. In canines, the nuclear genome contains 2.8 billion base pairs ...
IJBT 11(4) 412-415
... fresh leaf samples were ground in liquid nitrogen. Total genomic DNA was extracted from 2 g of powdered leaf tissue as described in Saghai-Maroof et al16. For PCR (polymerase chain reaction) amplification, DNA concentration of each sample was adjusted to 25 ng/µL by dissolving required quantities of ...
... fresh leaf samples were ground in liquid nitrogen. Total genomic DNA was extracted from 2 g of powdered leaf tissue as described in Saghai-Maroof et al16. For PCR (polymerase chain reaction) amplification, DNA concentration of each sample was adjusted to 25 ng/µL by dissolving required quantities of ...
Creating mutant flies
... Why would one mess around with this transposon, anyway? In the cross (= CROSS 3) you set up this week… what progeny phenotype would you want to pick for further analysis? Why? ...
... Why would one mess around with this transposon, anyway? In the cross (= CROSS 3) you set up this week… what progeny phenotype would you want to pick for further analysis? Why? ...
Protocadherin-1: epithelial barrier dysfunction in asthma and eczema Grissel Faura Tellez
... Caucasian subjects did not reveal strong evidence for LD between the various SNPs (fig. 1), indicating that these SNPs do not represent a single genetic signal but apparently make individual, independent contributions to disease susceptibility. Another explanation for the identification of multiple ...
... Caucasian subjects did not reveal strong evidence for LD between the various SNPs (fig. 1), indicating that these SNPs do not represent a single genetic signal but apparently make individual, independent contributions to disease susceptibility. Another explanation for the identification of multiple ...
The complete nucleotide sequence of the RNA coding for the
... was carried out as described by Rowlands et al (12). Briefly, a 5' P-labelled primer (Cell Tech. Ltd., UK) was used to direct the synthesis of cONA using reverse transcriptase and viral RNA as a template. The resulting 5' labelled cDNA was size fractionated and sequenced by the method of Maxam and G ...
... was carried out as described by Rowlands et al (12). Briefly, a 5' P-labelled primer (Cell Tech. Ltd., UK) was used to direct the synthesis of cONA using reverse transcriptase and viral RNA as a template. The resulting 5' labelled cDNA was size fractionated and sequenced by the method of Maxam and G ...
Loss of Function but No Gain of Function Caused by
... Homeodomain containing transcription factors of the Hox family play critical roles in patterning the anteroposterior embryonic body axis, as well as in controlling several steps of organogenesis. Several Hox proteins have been shown to cooperate with members of the Pbx family for the recognition and ...
... Homeodomain containing transcription factors of the Hox family play critical roles in patterning the anteroposterior embryonic body axis, as well as in controlling several steps of organogenesis. Several Hox proteins have been shown to cooperate with members of the Pbx family for the recognition and ...
PDF
... transferred to fresh ' M ' . The tentacle pattern of such animals was usually normal, i.e. 5-7 equal-length tentacles arranged radially around the hypostome. Occasionally 3 or 4 tentacles were shorter than normal but usually these increased in length to fit the normal pattern. None of these compound ...
... transferred to fresh ' M ' . The tentacle pattern of such animals was usually normal, i.e. 5-7 equal-length tentacles arranged radially around the hypostome. Occasionally 3 or 4 tentacles were shorter than normal but usually these increased in length to fit the normal pattern. None of these compound ...
Genetic Testing for Cancer Susceptibility
... Anthem UM Services, Inc., a separate company, is the licensed utilization review agent that performs utilization management services on behalf of your health benefit plan or the administrator of your health benefit plan. ...
... Anthem UM Services, Inc., a separate company, is the licensed utilization review agent that performs utilization management services on behalf of your health benefit plan or the administrator of your health benefit plan. ...
Integrated tRNA, transcript, and protein profiles in
... relied on organismal tRNA gene copy number as a proxy for tRNA abundance, and these results support this generalized relationship. We next asked whether tRNA levels are finely tuned toward codon usage, defined as the frequency in which a particular codon is used with respect to all codons. For most ...
... relied on organismal tRNA gene copy number as a proxy for tRNA abundance, and these results support this generalized relationship. We next asked whether tRNA levels are finely tuned toward codon usage, defined as the frequency in which a particular codon is used with respect to all codons. For most ...
Chapter 1
... what sort of molecules could have the kind of stability that the gene demanded, yet be capable of permanent, sudden change to the mutant forms that must provide the basis of evolution... It was generally assumed that genes would be composed of amino acids because, at that time, they appeared to be t ...
... what sort of molecules could have the kind of stability that the gene demanded, yet be capable of permanent, sudden change to the mutant forms that must provide the basis of evolution... It was generally assumed that genes would be composed of amino acids because, at that time, they appeared to be t ...
13.3 Mutations
... • There are four types of chromosomal mutations: deletion, duplication, inversion, and translocation. ...
... • There are four types of chromosomal mutations: deletion, duplication, inversion, and translocation. ...
The molecular epidemiology of iridovirus in Murray cod
... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
... arbitrarily chosen as a limit. If both primers in the pair had E-values of less than 1.0 for the same non-target DNA, they were excluded. Candidate primers for discriminatory assays, C50/C51 and C82/C83, were tested against DNA from MCIV, DGIV-2004, RSIV and EHNV. Primers (M68/ M69 and M151/M152) kn ...
Identification of Vietnamese Coptotermes pest species based on the
... The objectives of this study were to classify the Coptotermes found in certain provinces in Vietnam and assess the feasibility proposed PCR method by Szalanski et al., 2004 for identification of Coptotermes species. The proposed PCR method distinguishes species by the presence or absence of DNA frag ...
... The objectives of this study were to classify the Coptotermes found in certain provinces in Vietnam and assess the feasibility proposed PCR method by Szalanski et al., 2004 for identification of Coptotermes species. The proposed PCR method distinguishes species by the presence or absence of DNA frag ...
Biology Chapter 11 PRETEST
... c. the inheritance of traits. d. cross-pollination. 2. Offspring that result from crosses between true-breeding parents with different traits a. are true-breeding. b. make up the F2 generation. c. make up the parental generation. d. are called hybrids. 3. The chemical factors that determine traits a ...
... c. the inheritance of traits. d. cross-pollination. 2. Offspring that result from crosses between true-breeding parents with different traits a. are true-breeding. b. make up the F2 generation. c. make up the parental generation. d. are called hybrids. 3. The chemical factors that determine traits a ...
The Arabidopsis NAC Transcription Factor VNI2
... influenced by both developmental and environmental cues. Gene expression analysis using the GENEVESTIGATOR database (https://www.genevestigator.com/gv/index.jsp) revealed that VNI2, which is highly expressed in senescing plant tissues, is also induced by environmental stress conditions, suggesting t ...
... influenced by both developmental and environmental cues. Gene expression analysis using the GENEVESTIGATOR database (https://www.genevestigator.com/gv/index.jsp) revealed that VNI2, which is highly expressed in senescing plant tissues, is also induced by environmental stress conditions, suggesting t ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.