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Databases_what_and_w..
Databases_what_and_w..

... Intra and Inter-species relationships • Phenotypes come from the proteins. Niche exploitation Ecosystems • Proteins come from the DNA via RNA. • Changes in DNA cause changes in proteins. • Changes in proteins cause changes in phenotypes. ...
2001_butterfield_THE SUGARCANE GENOME
2001_butterfield_THE SUGARCANE GENOME

... marker order in grasses has been demonstrated over large chromosomal segments (Devos and Gale, 1997), several disease resistance genes are not well conserved and may prove difficult to identify through comparative analysis (Keller and Feuillet, 2000). In polyploids, study of single genes may be comp ...
Gene and Genotype frequencies
Gene and Genotype frequencies

... The genes carried in a population have continuity from generation to generation but not the genotypes in which they appear The genetic constitution of a population referring to genes it carries is described by Gene frequencies Gene frequencies can be determined from knowledge of genotype frequenc ...
5` Flanking Sequences of the Rat Tyrosine Hydroxylase Gene Target
5` Flanking Sequences of the Rat Tyrosine Hydroxylase Gene Target

... transgenic mice, where fine mapping is very difficult due to the time and expense involved in maintaining transgenic lines. Although limited in number, previous experiments in which TH regulatory regions were used in transgenic mice have failed to demonstrate correct tissue-specific expression, alth ...
Monitoring BCR-ABL transcript levels by real-time
Monitoring BCR-ABL transcript levels by real-time

... important to ensure that the conversion by our model does not change the clinical assessment of patients. If a major molecular response was considered as a BCRABL/ABL ratio less than 0.10, there were 21 samples with such a response according to both the calculated and estimated ratios. Furthermore, ...
Differential impact of APOBEC3-driven mutagenesis on HIV
Differential impact of APOBEC3-driven mutagenesis on HIV

Adverse Effects of Excessive Leucine Intake Depend on Dietary
Adverse Effects of Excessive Leucine Intake Depend on Dietary

... Summary  The present study was conducted to identify reliable gene biomarkers for the adverse effects of excessive leucine (Leu) in Sprague-Dawley rats by DNA microarray. It has long been known that the adverse effects of excessive amino acid intake depend on dietary protein levels. Male rats were d ...
Protein Synthesis and Words - Hewlett
Protein Synthesis and Words - Hewlett

... In the first step leading to protein synthesis, the nucleotide sequence of the DNA is transcribed (the process is called transcription) into a long single-stranded molecule of RNA, termed messenger RNA (mRNA). The mRNA moves out of the nucleus and into the cytoplasm through small pores in the nuclea ...
General - Bioinformatics Research Group at SRI International
General - Bioinformatics Research Group at SRI International

... denotes score for P in O  where: ...
Gene Signal Estimates from Exon Arrays v1.0
Gene Signal Estimates from Exon Arrays v1.0

... content on the array allows the detection of novel alternative events, but presents a new challenge. This is because including alternative exons can negatively affect the estimate for the entire transcriptional unit. Additionally, much of the content is exploratory in nature, such as the GENSCAN sub ...
Speaker: Dr. Arnob Dutta Title: Multiple mechanisms to regulate the
Speaker: Dr. Arnob Dutta Title: Multiple mechanisms to regulate the

Standard Genetic Nomenclature - Iowa State University Digital
Standard Genetic Nomenclature - Iowa State University Digital

... serological or nucleotide methods. The HGNC guideline recommends that 'allele designation should be written on the same line as gene symbol separated by an asterisk e.g. PGM1 * 1, the allele is printed as * 1 '. The wild-type allele can be denoted with a+ (e.g. MSTN+). Neither+ nor - symbols should ...
A. FF B. Ff C. ff
A. FF B. Ff C. ff

... Master ID: 25916 Revision: 1 Correct: D Rationale: A. Student(s) may have mixed up the terms "dominant" and "recessive," incorrectly thinking recessive alleles are usually written with capitalized letters. Student(s) may have believed that two dominant alleles cancel each other out. B. Student(s) ma ...
Zebrafish (Danio rerio) - Repositorio Académico
Zebrafish (Danio rerio) - Repositorio Académico

... because these models for human diseases can be used as testing assays for a desired biological activity, eg, recovery of cuproenzyme function in the mutant background. Mutations can be efficiently recovered in fish with chemicals that induce single nucleotide substitutions, the traditional way for m ...
Chapter 20 Biotechnology Multiple-Choice Questions
Chapter 20 Biotechnology Multiple-Choice Questions

... hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different med ...
Standard Genetic Nomenclature - Iowa State University Digital
Standard Genetic Nomenclature - Iowa State University Digital

... serological or nucleotide methods. The HGNC guideline recommends that 'allele designation should be written on the same line as gene symbol separated by an asterisk e.g. PGM1 * 1, the allele is printed as * 1 '. The wild-type allele can be denoted with a+ (e.g. MSTN+). Neither+ nor - symbols should ...
Allele Frequency Lab
Allele Frequency Lab

... medium neck length and would only be able to feed on the old, tough lower branches that have been picked over by the other giraffes. 3. A giraffe receiving two “short” alleles (two white beans) would have a shorter neck than the medium necked giraffe. This giraffe would not be able to reach over the ...
embj201489478-sup-0013-fig
embj201489478-sup-0013-fig

... gene structure 0.5 kb scale is shown. Bar graphs represent ChIP levels of P-Ser5 RNA PolII for alternatively spliced regions. Arrows on gene structure show localization of primers used for splicing analysis by RT-PCR and capillary electrophoresis, which is shown in tables. Tables represent splicing ...
Mobile genetic elements in antibiotic resistance
Mobile genetic elements in antibiotic resistance

... carry similar resistance mechanisms. e.g. R388 and R46, highly conserved regions are found in sequences immediately surrounding the structural gene. Flanking the structural gene are GTTA sequences which mark the insertion points for the resistance gene and represent recombination hot-spots necessary ...
The eukaryotic genome: a system regulated at different hierarchical
The eukaryotic genome: a system regulated at different hierarchical

... observations may be just the tip of the iceberg, since it is likely that regulation at the level of gene clusters primarily represents switching of chromatin domains from a transcriptionally repressive state (i.e. epigenetically silenced) to a state that is permissive for transcription (see the sect ...
Sequences of flavivirus-related RNA viruses persist in DNA form
Sequences of flavivirus-related RNA viruses persist in DNA form

... Flavivirus-related sequences have been discovered in the dsDNA genome of Aedes albopictus and Aedes aegypti mosquitoes, demonstrating for the first time an integration into a eukaryotic genome of a multigenic sequence from an RNA virus that replicates without a recognized DNA intermediate. In the Ae ...
Distinct patterns of collagen gene expression are seen in normal
Distinct patterns of collagen gene expression are seen in normal

... Northern blot analysis revealed two mRNA species of proa1(I) and proa1(III) collagens. These bands changed in parallel in both culture systems. It is of interest that in three-dimensional culture keloid fibroblasts showed a relative increase in the upper band (5·8 kb) at the expense of the lower ban ...
Leukaemia Section t(1;14)(q21;q32) MUC1/IGH Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(1;14)(q21;q32) MUC1/IGH Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Located 8cM telomeric to BCL9; aliases of MUC1 are EMA and CD227. DNA/RNA 3.88 kb, 8 exons, 1721 bp, 2 transcripts. Protein 122.1 kDa (1255 aa) Highly glycosylated protein. The MUC1 protein can be expressed as a transmembrane or secretedprotein. May be playing a role in adhesive functions and in cel ...
MOLLECULAR BIOLOGY COURSE
MOLLECULAR BIOLOGY COURSE

... DNA is transferred to a similar molecule called RNA (ribonucleic acid) in the cell nucleus. Both RNA and DNA are made up of a chain of nucleotide bases, but they have slightly different chemical properties. The type of RNA that contains the information for making a protein is called messenger RNA (m ...
Evolutionary Genomics of Fast Evolving Tunicates
Evolutionary Genomics of Fast Evolving Tunicates

... way to genome compaction in O. dioica is that the majority of genes contain very small introns. In fact, most introns are very short, being on average less than 50 nt in length, and only 2.4% of them are larger than 1 kb (Denoeud et al. 2010). Still, some genes have relatively large introns and inte ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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