Systemic methods for capturing protein–lipid interactions (PDF
... Competition consistent with known inhibitor selectivity ...
... Competition consistent with known inhibitor selectivity ...
The SR Protein SRp38 Represses Splicing in M Phase Cells
... of which approximately a dozen have been described in mammalian systems, are modular, containing one or two N-terminal RNP-type RNA binding domains (RBD) and a C-terminal region consisting largely of multiple Arg-Ser dipeptide repeats (RS domain). They play multiple roles in splicing and have the un ...
... of which approximately a dozen have been described in mammalian systems, are modular, containing one or two N-terminal RNP-type RNA binding domains (RBD) and a C-terminal region consisting largely of multiple Arg-Ser dipeptide repeats (RS domain). They play multiple roles in splicing and have the un ...
Diapositive 1
... were treated with the phosphatase inhibitor CalA (20 nM) and, at indicated time points, phosphorylated Par-4 (upward shift) was assessed by western blotting. (B) Cells were treated for 1h with CalA (20 nM) or okadaic acid (OA) both at a low (0.5 µM; shown to inhibit only phosphatase PP2) and at a hi ...
... were treated with the phosphatase inhibitor CalA (20 nM) and, at indicated time points, phosphorylated Par-4 (upward shift) was assessed by western blotting. (B) Cells were treated for 1h with CalA (20 nM) or okadaic acid (OA) both at a low (0.5 µM; shown to inhibit only phosphatase PP2) and at a hi ...
A new fusion hypothesis for the origin of Eukarya: better than
... archaebacteria) regain the primitive position that they have occupied in the tree of life from the XIX century (the Haeckel’s monera). In fusion scenarios, the three primary domains of Woese and colleagues are thus replaced by two primary “prokaryotic” domains, and “eukaryotes” therefore emerged onc ...
... archaebacteria) regain the primitive position that they have occupied in the tree of life from the XIX century (the Haeckel’s monera). In fusion scenarios, the three primary domains of Woese and colleagues are thus replaced by two primary “prokaryotic” domains, and “eukaryotes” therefore emerged onc ...
An Enhanced System for Unnatural Amino Acid
... three copies of tRNATyr CUA (Tyr) or tRNACUA (opt) and the pAcF-RS under the control of glnS′ (glnS'), araBAD (araB), or both (dual) were analyzed for their ability to suppress an amber mutant at position 151 in GFP in the presence (blue) or in the absence (red) of 1 mM pAcF. In one constructs, pAcF ...
... three copies of tRNATyr CUA (Tyr) or tRNACUA (opt) and the pAcF-RS under the control of glnS′ (glnS'), araBAD (araB), or both (dual) were analyzed for their ability to suppress an amber mutant at position 151 in GFP in the presence (blue) or in the absence (red) of 1 mM pAcF. In one constructs, pAcF ...
Calcium binding chaperones of the endoplasmic reticulum
... 2009). Calreticulin contains ER targeting signal sequence and it terminates with ER retrieval signal, Lys-Asp-Glu-LeuCOOH (KDEL) (Fliegel et al. 1989). The protein has been implicated to play a role in many diverse cellular processes (Michalak et al. 2009). Many of these functions are due to calreti ...
... 2009). Calreticulin contains ER targeting signal sequence and it terminates with ER retrieval signal, Lys-Asp-Glu-LeuCOOH (KDEL) (Fliegel et al. 1989). The protein has been implicated to play a role in many diverse cellular processes (Michalak et al. 2009). Many of these functions are due to calreti ...
Glycerolipid transfer for the building of membranes in plant cells.
... movement through a lipid bilayer takes less than one second for DAG but it takes hours for lipids with larger polar heads [12]. Lipid diffusion between different membranes is fast for single fatty acid chain lipids that easily partition into the aqueous phase, like lysoPC, or lipids containing two ...
... movement through a lipid bilayer takes less than one second for DAG but it takes hours for lipids with larger polar heads [12]. Lipid diffusion between different membranes is fast for single fatty acid chain lipids that easily partition into the aqueous phase, like lysoPC, or lipids containing two ...
Proteins RCA Gene Cluster and Functional RCA Locus in Chicken
... activation (RCA) locus. Each SCR consists of 60 –70 aa, including 4 highly conserved cysteines (3, 4). The cysteines form disulfide bonds, folding the SCRs into a rigid triple-loop structure (3, 4). Although the proteins in the RCA family vary in size, they share significantly similar in three-dimen ...
... activation (RCA) locus. Each SCR consists of 60 –70 aa, including 4 highly conserved cysteines (3, 4). The cysteines form disulfide bonds, folding the SCRs into a rigid triple-loop structure (3, 4). Although the proteins in the RCA family vary in size, they share significantly similar in three-dimen ...
Endothelial cell response to different mechanical forces
... In our studies, only JNK was activated with a similar magnitude and temporal pattern by both shear stress and cyclic strain (both with peak at 30 minutes). This suggests that it is possible that the mechanoreceptor and upstream pathway leading to JNK activation for shear stress may be similar to tho ...
... In our studies, only JNK was activated with a similar magnitude and temporal pattern by both shear stress and cyclic strain (both with peak at 30 minutes). This suggests that it is possible that the mechanoreceptor and upstream pathway leading to JNK activation for shear stress may be similar to tho ...
The Role of Cyclin B in Meiosis I
... of the mitotic cell cycles (Masui and Clarke, 1979). However, in contrast to somatic ceils, most embryonic cells have abbreviated cell cycles consisting of rapid, alternating periods of DNA synthesis (interphase) and mitosis (M phase). The G1 and G2 periods are absent or very short in these "cleavag ...
... of the mitotic cell cycles (Masui and Clarke, 1979). However, in contrast to somatic ceils, most embryonic cells have abbreviated cell cycles consisting of rapid, alternating periods of DNA synthesis (interphase) and mitosis (M phase). The G1 and G2 periods are absent or very short in these "cleavag ...
Mapping the Synthetic Dosage Lethality Network of CDK1/CDC28
... an essential cellular process (Dixon et al. 2009). Therefore, synthetic lethality can be used to identify new regulators of specific cellular processes, or assign new functions to genes (Enserink 2012). For instance, we recently reported an SGA screen in which we used low doses of 1-NM-PP1 that part ...
... an essential cellular process (Dixon et al. 2009). Therefore, synthetic lethality can be used to identify new regulators of specific cellular processes, or assign new functions to genes (Enserink 2012). For instance, we recently reported an SGA screen in which we used low doses of 1-NM-PP1 that part ...
Document
... Phenylpropanoid biosynthesis in plants engenders a vast variety of aromatic metabolites critically important for their growth, development, and environmental adaptation. Some of these aromatic compounds have high economic value. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the ...
... Phenylpropanoid biosynthesis in plants engenders a vast variety of aromatic metabolites critically important for their growth, development, and environmental adaptation. Some of these aromatic compounds have high economic value. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the ...
Boundless Study Slides
... • biofilm a thin film of mucus created by and containing a colony of bacteria and other microorganisms • biofilm a thin film of mucus created by and containing a colony of bacteria and other microorganisms • cyclic adenosine monophosphate cAMP, a second messenger derived from ATP that is involved in ...
... • biofilm a thin film of mucus created by and containing a colony of bacteria and other microorganisms • biofilm a thin film of mucus created by and containing a colony of bacteria and other microorganisms • cyclic adenosine monophosphate cAMP, a second messenger derived from ATP that is involved in ...
Podosome-type adhesions and focal adhesions, so alike yet so
... sites so close in their composition can be structurally and functionally so different. The underlying question is how their respective physiological or pathological roles are related to their distinct organization. ...
... sites so close in their composition can be structurally and functionally so different. The underlying question is how their respective physiological or pathological roles are related to their distinct organization. ...
Production and Characterization of Islet Amyloid Polypeptide Using
... In previously conducted research, islets taken from T2DM patients have been found to secrete less insulin and could not normalize the level of glucose in diabetic animals into which these islets were transplanted; however, it is important to note that the response obtained from the glucagon hormone ...
... In previously conducted research, islets taken from T2DM patients have been found to secrete less insulin and could not normalize the level of glucose in diabetic animals into which these islets were transplanted; however, it is important to note that the response obtained from the glucagon hormone ...
The Ph1 Locus Suppresses Cdk2-Type Activity during Premeiosis
... sequence similarity to mammalian Cdk2 (Griffiths et al., 2006; AlKaff et al., 2008; Yousafzai et al., 2010a). Cdks are a group of Ser/ Thr protein kinases that control the progression through the various phases of the cell cycle and subsequent cell divisions in eukaryotic cells. Cdk2 has been shown ...
... sequence similarity to mammalian Cdk2 (Griffiths et al., 2006; AlKaff et al., 2008; Yousafzai et al., 2010a). Cdks are a group of Ser/ Thr protein kinases that control the progression through the various phases of the cell cycle and subsequent cell divisions in eukaryotic cells. Cdk2 has been shown ...
Bradykinin stimulates IL-6 and IL-8 production by human lung
... However, the intracellular signalling mechanisms by which BK induced proinflammatory cytokine production have not been fully elucidated. This study investigated the role of the extracellular signal-regulated protein kinase 1/2 (ERK 1/2) and p38 mitogen-activated protein kinase (p38 MAPK) in the BK-i ...
... However, the intracellular signalling mechanisms by which BK induced proinflammatory cytokine production have not been fully elucidated. This study investigated the role of the extracellular signal-regulated protein kinase 1/2 (ERK 1/2) and p38 mitogen-activated protein kinase (p38 MAPK) in the BK-i ...
Mercury, Cadmium, and Arsenite Enhance Heat Shock Protein
... Fig. 1. A: Dose-dependent changes in de novo stress protein synthesis induced in chick embryos exposed to metals. Representative SDSPAGE (12.5% minigels) profiles of 35S-methionine-labeled chick embryonic proteins 2 h after exposure to 0 (Con), 3, 10, 30, or 100 nmol/ embryo of arsenite (As), cadmiu ...
... Fig. 1. A: Dose-dependent changes in de novo stress protein synthesis induced in chick embryos exposed to metals. Representative SDSPAGE (12.5% minigels) profiles of 35S-methionine-labeled chick embryonic proteins 2 h after exposure to 0 (Con), 3, 10, 30, or 100 nmol/ embryo of arsenite (As), cadmiu ...
Regulation of tubulin heterodimer partitioning during interphase and
... counteracted by MAP4. These studies also established Op18 and MAP4 as the predominant regulators of tubulin subunit partitioning in all three human cell model systems studied. Moreover, consistent with phosphorylation-inactivation of these two proteins during mitosis, we found that the microtubule-r ...
... counteracted by MAP4. These studies also established Op18 and MAP4 as the predominant regulators of tubulin subunit partitioning in all three human cell model systems studied. Moreover, consistent with phosphorylation-inactivation of these two proteins during mitosis, we found that the microtubule-r ...
29.9 Tanaka
... Another intriguing possibility offered by macromolecular spacers is the attachment of large proteins or protein complexes to supported membranes. If the proteins are attached such that they retain lateral mobility while avoiding non-specific attractive van der Waals contact with the model membrane, ...
... Another intriguing possibility offered by macromolecular spacers is the attachment of large proteins or protein complexes to supported membranes. If the proteins are attached such that they retain lateral mobility while avoiding non-specific attractive van der Waals contact with the model membrane, ...
Translocation of Globin Fusion Proteins across the Endoplasmic
... K, some with 1% Triton (lanes G and J) added. Samples were immunoprecipitated either with anti-globin (lanes A, B, E-G) or antiprolactin (lanes C, D, H-J) antiserum and subjected to SDS PAGE. Gels were fluorographed, and bands were visualized by autoradiography. Large arrowheads indicate mature prol ...
... K, some with 1% Triton (lanes G and J) added. Samples were immunoprecipitated either with anti-globin (lanes A, B, E-G) or antiprolactin (lanes C, D, H-J) antiserum and subjected to SDS PAGE. Gels were fluorographed, and bands were visualized by autoradiography. Large arrowheads indicate mature prol ...
Structural studies on lysosomal proteins
... which is beneficial for the hydrolytic processes. In addition, since transport of molecules to lysosomes is highly controlled, cells can regulate what is hydrolysed and when the hydrolysis takes place. Thus, degradation can be used both for recycling and regulation purposes. The lysosomal lumen is n ...
... which is beneficial for the hydrolytic processes. In addition, since transport of molecules to lysosomes is highly controlled, cells can regulate what is hydrolysed and when the hydrolysis takes place. Thus, degradation can be used both for recycling and regulation purposes. The lysosomal lumen is n ...
consequences for amino acid starvation.
... Proteins form the basis of all biological processes within a cell, and one of the key characteristics of life is the ability of an organism to synthesize its own proteins. Understanding how a cell regulates this synthesis remains one of the key questions of modern science. In this paper we propose a ...
... Proteins form the basis of all biological processes within a cell, and one of the key characteristics of life is the ability of an organism to synthesize its own proteins. Understanding how a cell regulates this synthesis remains one of the key questions of modern science. In this paper we propose a ...
The Amino-terminal Domain of the Golgi Protein Giantin Interacts
... matrix protein 130; PAGE, polyacrylamide gel electrophoresis; kb, kilobase(s); PCR, polymerase chain reaction; MALDI, matrix-assisted laser desorption and ionization. This paper is available on line at http://www.jbc.org ...
... matrix protein 130; PAGE, polyacrylamide gel electrophoresis; kb, kilobase(s); PCR, polymerase chain reaction; MALDI, matrix-assisted laser desorption and ionization. This paper is available on line at http://www.jbc.org ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.