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... Basel). Raeppli allows whole-tissue labeling such that the descendants of the majority of cells in a single organ are labeled and can be followed simultaneously relative to one another. We tested the use of Raeppli in the Drosophila melanogaster wing imaginal disc. Induction of Raeppli during larval ...
... Basel). Raeppli allows whole-tissue labeling such that the descendants of the majority of cells in a single organ are labeled and can be followed simultaneously relative to one another. We tested the use of Raeppli in the Drosophila melanogaster wing imaginal disc. Induction of Raeppli during larval ...
Membrane Trafficking During Plant Cytokinesis
... Formation of transport vesicles involves the assembly of distinct coat complexes that drive membrane budding and the selection of cargo proteins. This process is regulated by small GTPases such as ARF (ADP-Ribosylation Factor) that are required for the formation of TGN-derived clathrin coated vesicl ...
... Formation of transport vesicles involves the assembly of distinct coat complexes that drive membrane budding and the selection of cargo proteins. This process is regulated by small GTPases such as ARF (ADP-Ribosylation Factor) that are required for the formation of TGN-derived clathrin coated vesicl ...
Plant Cell Walls
... partial hydrolysis of wall polysaccharides, possess hormone-like or signalling activity, regulating growth, development and metabolism. Such oligosaccharides are termed oligosaccharins. For instance, xyloglucan-derived oligosaccharins can regulate cell expansion, and pectin-derived oligosaccharins c ...
... partial hydrolysis of wall polysaccharides, possess hormone-like or signalling activity, regulating growth, development and metabolism. Such oligosaccharides are termed oligosaccharins. For instance, xyloglucan-derived oligosaccharins can regulate cell expansion, and pectin-derived oligosaccharins c ...
Anticancer Effects of the Organosilicon Multidrug Resistance
... gene expression of SILA 421-treated HL-60 cells was employed to identify cellular pathways affected by the compound and revealed disturbance of DNA replication, transcription and production of apparently misfolded proteins. Endoplasmatic reticulum stress and downregulation of cell cycle, cellular re ...
... gene expression of SILA 421-treated HL-60 cells was employed to identify cellular pathways affected by the compound and revealed disturbance of DNA replication, transcription and production of apparently misfolded proteins. Endoplasmatic reticulum stress and downregulation of cell cycle, cellular re ...
Beyond apoptosis: nonapoptotic cell death in physiology and disease
... necrosis, which is a passive process. Such distinction is essential because both forms of cell death are phenotypically alike, but are distinct from apoptosis, and are probably caused by different mechanisms (Jaattela and Tschopp 2003). In particular, at late stages of both accidental necrosis and n ...
... necrosis, which is a passive process. Such distinction is essential because both forms of cell death are phenotypically alike, but are distinct from apoptosis, and are probably caused by different mechanisms (Jaattela and Tschopp 2003). In particular, at late stages of both accidental necrosis and n ...
Exploring How Zika Virus Attacks Cells in Developing Brains
... the early stages of infection. Collectively known as the secretome, these proteins include growth factors, infectionfighting proteins, and bioactive molecules critical for differentiating stem cells into specific cell types. In this study, over a period of seven days, researchers charted the genetic ...
... the early stages of infection. Collectively known as the secretome, these proteins include growth factors, infectionfighting proteins, and bioactive molecules critical for differentiating stem cells into specific cell types. In this study, over a period of seven days, researchers charted the genetic ...
Jeopardy
... -Ribosomes start to process the protein -Rough ER and ribosomes continue to process proteins -Protein passes through the smooth ER, and is Transported in a vesicle to the Golgi body -The Golgi finalizes the protein and modifies its structure, so it is now a functional protein, which is transported i ...
... -Ribosomes start to process the protein -Rough ER and ribosomes continue to process proteins -Protein passes through the smooth ER, and is Transported in a vesicle to the Golgi body -The Golgi finalizes the protein and modifies its structure, so it is now a functional protein, which is transported i ...
Permanent cell cycle exit in G2 phase after DNA damage in normal
... MEFs as models. We purposely did not use experimental systems based on tumour-derived cells lines, as many of them have acquired uncharacterized genetic abnormalities, which can confound the interpretation of the data obtained. Moreover, the cell lines we tested exhibited aberrant post-mitotic ®gure ...
... MEFs as models. We purposely did not use experimental systems based on tumour-derived cells lines, as many of them have acquired uncharacterized genetic abnormalities, which can confound the interpretation of the data obtained. Moreover, the cell lines we tested exhibited aberrant post-mitotic ®gure ...
Translation of Human-Induced Pluripotent€Stem Cells
... tional Institutes of Health (NIH; R01 HL126527, NIH R01 HL123968, NIH R01 HL128170, and NIH R01 HL130020) to Dr. Wu, and by an American Heart Association scientist development grant (13SDG17340025), NIH-National Heart, Lung, and Blood Institute Progenitor Cell Biology Consortium pilot grant (SR00003 ...
... tional Institutes of Health (NIH; R01 HL126527, NIH R01 HL123968, NIH R01 HL128170, and NIH R01 HL130020) to Dr. Wu, and by an American Heart Association scientist development grant (13SDG17340025), NIH-National Heart, Lung, and Blood Institute Progenitor Cell Biology Consortium pilot grant (SR00003 ...
Insulin-like Growth Factor I Receptor Signaling in
... epidermal growth factor or serum and differentiate to a neuronal phenotype in N2 medium supplemented with bFGF at the nonpermissive temperature of 39°C (12, 13). We infected H19-7 cells with a retroviral vector expressing the human IGF-IR, and after selection, the mixed population was tested by West ...
... epidermal growth factor or serum and differentiate to a neuronal phenotype in N2 medium supplemented with bFGF at the nonpermissive temperature of 39°C (12, 13). We infected H19-7 cells with a retroviral vector expressing the human IGF-IR, and after selection, the mixed population was tested by West ...
A High Throughput, Whole Cell Screen for Small
... or chemically induced defects in the mitotic spindle can lead to long term activation of the spindle checkpoint, whereupon cells arrest at the preanaphase stage for many hours. Many cancers show abnormal chromosome content, often being hyperdiploid. Certain lines derived from tumors have abnormal bu ...
... or chemically induced defects in the mitotic spindle can lead to long term activation of the spindle checkpoint, whereupon cells arrest at the preanaphase stage for many hours. Many cancers show abnormal chromosome content, often being hyperdiploid. Certain lines derived from tumors have abnormal bu ...
Differentiation of Mesenchymal Stem Cells Into Dopaminergic
... by IL-1β or GDNF, so the results about the two experimental groups were not discussed. The analysis of immunocytochemistry showed that there were various numbers of NSE, MAP-2a, b and TH-positive cells in the other experimental groups. The bodies of round or oval- shape positive cells were smaller t ...
... by IL-1β or GDNF, so the results about the two experimental groups were not discussed. The analysis of immunocytochemistry showed that there were various numbers of NSE, MAP-2a, b and TH-positive cells in the other experimental groups. The bodies of round or oval- shape positive cells were smaller t ...
Microinjection into plant cells of etiolated - Products
... incubated for various times in continuous darkness (dD) after germination (Sinapis alba 4 dD, Lycopersicon esculentum 6 dD, Arabidopsis thaliana 4 dD). Germination of Arabidopsis seedlings was induced by 2 days of cold treatment (4°C) and subsequent irradiation with white light for 6 h. Prior to mic ...
... incubated for various times in continuous darkness (dD) after germination (Sinapis alba 4 dD, Lycopersicon esculentum 6 dD, Arabidopsis thaliana 4 dD). Germination of Arabidopsis seedlings was induced by 2 days of cold treatment (4°C) and subsequent irradiation with white light for 6 h. Prior to mic ...
Understanding Our Environment
... - phospholipid bilayer with embedded proteins (transport proteins and receptor proteins) 2. Genetic material is enclosed within a nucleus or a nucleoid 3. Cytoplasm Raven - Johnson - Biology: 6th Ed. - All Rights Reserved - McGraw Hill Companies ...
... - phospholipid bilayer with embedded proteins (transport proteins and receptor proteins) 2. Genetic material is enclosed within a nucleus or a nucleoid 3. Cytoplasm Raven - Johnson - Biology: 6th Ed. - All Rights Reserved - McGraw Hill Companies ...
The Human Body in Health and Illness
... • Endoplasmic reticulum (ER) – Network of membranes in cytosol – Rough endoplasmic reticulum (RER): Fixed ribosomes on surface – Smooth endoplasmic reticulum (SER): No ribosomes; site of lipid and steroid synthesis Copyright © 2011, 2007 by Saunders, an imprint of Elsevier Inc. All rights reserved. ...
... • Endoplasmic reticulum (ER) – Network of membranes in cytosol – Rough endoplasmic reticulum (RER): Fixed ribosomes on surface – Smooth endoplasmic reticulum (SER): No ribosomes; site of lipid and steroid synthesis Copyright © 2011, 2007 by Saunders, an imprint of Elsevier Inc. All rights reserved. ...
IP3R-mediated Ca2+ release regulates protein metabolism in
... and Hasan, 2009). Thus, it is possible that a signaling cascade connects SOCE to protein translation, via IP3R. Unlike NE cells, the rate of protein translation in itprku neurons was found to be no different from control neurons (Fig. S4A,B), suggesting that IP3R compensation of InR signaling is cel ...
... and Hasan, 2009). Thus, it is possible that a signaling cascade connects SOCE to protein translation, via IP3R. Unlike NE cells, the rate of protein translation in itprku neurons was found to be no different from control neurons (Fig. S4A,B), suggesting that IP3R compensation of InR signaling is cel ...
FYVE-dependent endosomal targeting of an arrestin-related
... important in vivo and mechanistic insights have been unveiled in alternative models such as flies, zebrafish, worms and more recently fungi [5–12]. The social soil amoeba Dictyostelium discoideum is an attractive model system for use in studying the regulation of membrane trafficking events: it is a ...
... important in vivo and mechanistic insights have been unveiled in alternative models such as flies, zebrafish, worms and more recently fungi [5–12]. The social soil amoeba Dictyostelium discoideum is an attractive model system for use in studying the regulation of membrane trafficking events: it is a ...
Primordial germ cell migration - The International Journal of
... narrowly defined track. PGCs lacking β1 integrin were unable to colonize the genital ridges demonstrating that interactions between the PGCs and the ECM are vital for this process (Anderson et al., 1999). However, transplantation experiments have recently shown that ectopically placed PGCs still mov ...
... narrowly defined track. PGCs lacking β1 integrin were unable to colonize the genital ridges demonstrating that interactions between the PGCs and the ECM are vital for this process (Anderson et al., 1999). However, transplantation experiments have recently shown that ectopically placed PGCs still mov ...
Muscle Cells - Circulation Research
... Downloaded from http://circres.ahajournals.org/ by guest on June 18, 2017 ...
... Downloaded from http://circres.ahajournals.org/ by guest on June 18, 2017 ...
Posters – Immunology and Oncology NAME OF THE PROJECT
... Key words : GvHD, T Lymphocyte, Diagnostic kit, Prediction kit, Transplantation, Cell culture, HSCT ...
... Key words : GvHD, T Lymphocyte, Diagnostic kit, Prediction kit, Transplantation, Cell culture, HSCT ...
Assays for Cell Enumeration, Cell Proliferation and Cell Cycle
... corresponding ribonucleotide (BrUTP, B21551) that has been microinjected into cells is incorporated into RNA of a nucleolar compartment, a process that should also be detectable with fluorescent anti-BrdU conjugates. ABSOLUTE-S SBIP Cell Proliferation Assay Kit Many conventional BrdU-based protocols ...
... corresponding ribonucleotide (BrUTP, B21551) that has been microinjected into cells is incorporated into RNA of a nucleolar compartment, a process that should also be detectable with fluorescent anti-BrdU conjugates. ABSOLUTE-S SBIP Cell Proliferation Assay Kit Many conventional BrdU-based protocols ...
Myeloid RelA regulates pulmonary host defense networks and R. Bals*
... myeloid NF-kB resulted in a significant increased number of viable bacterial in the lung homogenates compared with the wild-type (WT) mice (fig. 1a) with suppressed influx of inflammatory cells (fig. 1b). The relative number of individual cell types (macrophages, neutrophils and lymphocytes) was not ...
... myeloid NF-kB resulted in a significant increased number of viable bacterial in the lung homogenates compared with the wild-type (WT) mice (fig. 1a) with suppressed influx of inflammatory cells (fig. 1b). The relative number of individual cell types (macrophages, neutrophils and lymphocytes) was not ...
Cell Membrane Structure and Function
... 1.Transport. (left) A protein that spans the membrane may provide a hydrophilic channel across the membrane that is selective for a particular solute. (right) Other transport proteins shuttle a substance from one side to the other by changing shape. Some of these proteins hydrolyze ATP as an energy ...
... 1.Transport. (left) A protein that spans the membrane may provide a hydrophilic channel across the membrane that is selective for a particular solute. (right) Other transport proteins shuttle a substance from one side to the other by changing shape. Some of these proteins hydrolyze ATP as an energy ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.