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Extended PDF
Extended PDF

... cells is increasing over time. Larger fields of the same region are shown in Figure S1. The scale bar represents 1 mm. (D) Average fluorescence intensity of the gfp cells as a function of their distance from the gfp+ cells at t0 min (light blue bars) and at t60 min (dark blue bars) of the coincubat ...
Synaptic Regulation of the Light-Dependent Oscillatory Currents in
Synaptic Regulation of the Light-Dependent Oscillatory Currents in

... seven main dendritic branches that formed a proximal zone, which then divided into thinner intermediate segments that divided further into a plexus of distal branches showing numerous varicosities (Fig. 1A). As we have described previously, mouse starburst cells displayed a number of basic and stere ...
Vacuolar Function in the Phosphate Homeostasis of the Yeast
Vacuolar Function in the Phosphate Homeostasis of the Yeast

... NMR, we examined the changes of intracellular phosphorous metabolites both in the cytosol and the vacuole under the phosphate starvation. Wild-type (YW5-1B) cells were grown to exponential phase in SD medium, and transferred to SD(—P^ medium, then the amounts of intracellular phosphorous compounds w ...
Targeted disruption of fibroblast growth factor receptor
Targeted disruption of fibroblast growth factor receptor

... primitive streak and other axial structures (Deng et al., 1994; Yamaguchi et al., 1994). Similarly, targeted disruption of FGFR-2 leads to abortion of the egg cylinder and dominant negative homozygotes die early after implantation of blastocysts (Arman et al., 1998). In contrast, FGF-2-deficient mic ...
Terminal Cytokinesis Events Uncovered after an RNAi Screen
Terminal Cytokinesis Events Uncovered after an RNAi Screen

... separation of daughter cells. Rather, as first described by Flemming in 1891 (see [7]), a persistent intercellular bridge forms around the spindle remnant; this bridge is marked at its center by a darkly staining structure, or midbody (e.g., Figure 1A, insert). This bridge remains long after furrowi ...
Inducing Embryonic Stem Cells to Become
Inducing Embryonic Stem Cells to Become

... endothelial cells, veins, interstitial fibroblasts, nodal cells, conduction system cells, working cardiomyocytes, etc.). Advances in surgical and pharmacologic interventions, as well as the development of electrophysiologic and mechanical devices, have steadily advanced and currently provide a wide ...
Genetic transformation of HeLa cells by Agrobacterium - igem
Genetic transformation of HeLa cells by Agrobacterium - igem

... Agrobacterium toward wounded sites on the host plant, attachment of the bacteria to the plant cell surface, and activation of the T-DNA transfer machinery (5, 6). During the attachment, Agrobacterium first loosely binds to the plant surface, and then it produces cellulose fibrils that tighten the bi ...
Remapping by hippocampal place cells
Remapping by hippocampal place cells

... Taking a slightly different view, in their elegant discussion of spatial context, Nadel and Willner (1980) argue that context is paradoxical: it both “is made up of” and “contains” the same stimuli. In other words, a given stimulus could be either a discrete cue or part of the context, depending on ...
Invasion of bone cells by Staphylococcus epidermidis
Invasion of bone cells by Staphylococcus epidermidis

... relatively few antibiotics penetrate into the cytoplasm of these cells and kill intracellular S. aureus [15]. Invasion of bone cells by S. aureus occurs via a receptormediated pathway that requires the involvement of host cell cytoskeletal elements [13,16]. The receptor on the surface of bone cells ...
Cytokinin Functions
Cytokinin Functions

... associates (Miller et al., 1955). This compound was named kinetin because of its ability to promote cytokinesis. Hall and deRopp reported that kinetin could be formed from DNA degradation products in 1955 (Hall and deRopp, 1955). 1961: Miller isolated the first naturally occurring cytokinin from cor ...
Guidelines for Building Self-Organizing Maps
Guidelines for Building Self-Organizing Maps

... of observations.  Cells adjust their weights with each  placement by becoming more similar to cells in their  immediate vicinity which have had assignments. – Create a list of observations with values on a number of  variables (dimensions) – Compute the similarity among observations based on all  va ...
Cyclin Dependent Kinases and Cell Cycle Control
Cyclin Dependent Kinases and Cell Cycle Control

... thought only to act at the G1兾S boundary in budding yeast, although a cdc28ts mutant had been described which became blocked at mitosis (Piggott et al., 1982). We proposed that cdc2兾CDC28 acted at both the G1兾S and G2兾M transitions in both yeasts, but in budding yeast it had been difficult to detect ...
Downloaded - The Journal of Cell Biology
Downloaded - The Journal of Cell Biology

... 5 kD that we have previously purified from granules of bovine neutrophils (11). These peptides have been called bactenecins, from the Latin words bacterium and necare (to kill), and are designated Bac7 and Bac5 in an abbreviated form. The basic tools in our studies have been specific antibodies rais ...
Document
Document

... Supplementary Fig. S4. Canine macrophages respond to CD47-blocking therapies in vitro. CD14+ monocytes were purified from canine blood samples and differentiated ex vivo to macrophages by culture in 10% autologous canine serum. A Representative images of canine macrophages by phase contrast microsco ...
Immunity Hemopoiesis and Cellular Drosophila
Immunity Hemopoiesis and Cellular Drosophila

... necessary for hemocyte migration toward a wound. They also show that Pvr signaling is not involved in chemotaxis toward a wound. In mammals, PI3K can induce Rac leading to lamellipodia formation and is proposed to feedback to activate PI3K. It would be interesting to see whether a similar mechanism ...
Lecture 14, Secondary growth in stems and roots
Lecture 14, Secondary growth in stems and roots

... Fate of the epidermis and cuticle is to be sloughed off the outside of the stem as phellem (cork) is produced ...
Treatment of experimental arthritis by inducing immune tolerance
Treatment of experimental arthritis by inducing immune tolerance

... Treatment was begun after the onset of disease, when arthritis had become well established (arthritis score ⬎2) or severe (arthritis score ⬎4). Mice with CIA were injected intraperitoneally each day for 5 days with phosphate buffered saline (PBS; control) or with PBS containing 106 human AD-MSCs, al ...
Stomata lab data sheet
Stomata lab data sheet

... Lab Experiment for Section 5.2 Purpose: In this activity, you will be investigating the structure of the stomata within a spinach plant leaf and determine how it functions with regards to the process of photosynthesis. Objectives:  Students will be able to define the function of the stomata  Stude ...
769_2006_146_MOESEM1_ESM
769_2006_146_MOESEM1_ESM

as a PDF
as a PDF

... post-irradiation, respectively. M-CSF is a key regulator of proliferation, survival and differentiation of monocytes/macrophages, whereas MCP-1 functions in the attraction of monocytes, granulocytes and lymphocytes. Thus, both chemokines may promote the influx of immune cells, particularly macrophag ...
Nanoparticle Delivery of siRNA as a Novel Therapeutic for Human
Nanoparticle Delivery of siRNA as a Novel Therapeutic for Human

Suppression by Filarial Parasites Not IL
Suppression by Filarial Parasites Not IL

... the appropriate concentration of anti-macrophage (Clone F4/80, R-phycoerythrin-conjugated, Caltag Laboratories, San Francisco, CA) mAb, as determined by titration. F4/80-positive and -negative PEC were sorted using a FACStar (Becton Dickinson, San Jose, CA), with logarithmic amplification of fluores ...
Chapter 12 The Cell Cycle Multiple-Choice Questions
Chapter 12 The Cell Cycle Multiple-Choice Questions

... Skill: Knowledge/Comprehension ...
Saquinavir, an HIV Protease Inhibitor, Is Transported by P
Saquinavir, an HIV Protease Inhibitor, Is Transported by P

... al., 1995). Exposure of these cells to vinblastine or doxorubicin demonstrates a 27-fold resistance to vinblastine and an 11-fold resistance to doxorubicin (fig. 2), which is consistent with previous observations for these cells (Currier et al., 1992; Cardarelli et al., 1995). Western immunoblot ana ...
Phenotypic Modulation of Smooth Muscle Cells
Phenotypic Modulation of Smooth Muscle Cells

... genes encoding smooth muscle (SM)-specific contractile proteins and extracellular matrix proteins. Fully differentiated SMCs express the highest levels of SM contractile proteins such as SM α-actin, SM22α, SM myosin light chain kinase (SM-MLCK), SM-myosin heavy chain (SM-MHC), and SM-calponin, which ...
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Cell encapsulation



Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.
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